Article
Spectroscopy
Ewelina Matuszyk, Adriana Adamczyk, Basseem Radwan, Anna Pieczara, Piotr Szczesniak, Jacek Mlynarski, Katarzyna Kaminska, Malgorzata Baranska
Summary: Raman imaging with molecular reporters is a novel method for subcellular visualization, offering improved selectivity and sensitivity; this study aimed to visualize organelles in endothelial cells using established and new reporters, and to investigate changes in organelle distribution after induced dysfunction, highlighting drawbacks of established reporters.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Inorganic & Nuclear
Haitao Yu, Yan Guo, Wencheng Zhu, Kaden Havener, Xujun Zheng
Summary: This review highlights the recent advances in the imaging and tracking of organelles in living cells using 1,8-naphthalimide (NI) fluorophore, focusing on their labeling mechanism and applications for monitoring various aspects of the cell microenvironment. It also discusses the limitations of the reported NI-based organelle fluorescent probes and offers insights into future developments in this field. The review aims to serve as a reference for beginners and a guide for chemical and biological researchers in exploring more promising research topics.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Spectroscopy
Feng-Tao Yu, Ze Huang, Jia-Xiang Yang, Long-Mei Yang, Xian-Yun Xu, Jian-Yan Huang, Lin Kong
Summary: Two viscosity-sensitive fluorescent probes were designed and synthesized to localize in lysosome and mitochondria in living HeLa cells, respectively. The fluorescence quantum yield and two-photon absorption cross section of the probes were significantly increased with the increase of viscosity. Fluorescence lifetime imaging of the cells stained with the probes revealed the viscosity range of lysosome and mitochondria. This type of probe is of great importance for monitoring diseases associated with abnormal viscosity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Nicholas L. Turner, Thomas Macrina, J. Alexander Bae, Runzhe Yang, Alyssa M. Wilson, Casey Schneider-Mizell, Kisuk Lee, Ran Lu, Jingpeng Wu, Agnes L. Bodor, Adam A. Bleckert, Derrick Brittain, Emmanouil Froudarakis, Sven Dorkenwald, Forrest Collman, Nico Kemnitz, Dodam Ih, William M. Silversmith, Jonathan Zung, Aleksandar Zlateski, Ignacio Tartavull, Szi-chieh Yu, Sergiy Popovych, Shang Mu, William Wong, Chris S. Jordan, Manuel Castro, JoAnn Buchanan, Daniel J. Bumbarger, Marc Takeno, Russel Torres, Gayathri Mahalingam, Leila Elabbady, Yang Li, Erick Cobos, Pengcheng Zhou, Shelby Suckow, Lynne Becker, Liam Paninski, Franck Polleux, Jacob Reimer, Andreas S. Tolias, R. Clay Reid, Nuno Macarico da Costa, H. Sebastian Seung
Summary: This article describes a model reconstructed from electron microscopic images of mouse primary visual cortex, and investigates the relationship between cell organization and function. The study suggests that the connectivity pattern of pyramidal cells is associated with their visual response strength and reliability.
Article
Pharmacology & Pharmacy
Haifeng Hu, Peng Li, Jie Qiu, Meiji Zhao, Mingjie Kuang, Zhaoyan Zhang, Dachuan Wang
Summary: This study used two-photon laser confocal microscopy to analyze the uptake and subcellular distribution of red fluorescent graphene quantum dots (Red-GQDs) in HeLa cells and discussed their effects on cell metabolism. The results showed that Red-GQDs were well-absorbed by HeLa cells and distributed in lysosomes and mitochondria in a time-dependent manner. Additionally, Red-GQDs significantly affected cell functions, inducing autophagy at low concentrations and promoting cell death and exosome secretion at high concentrations.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Yujuan Fu, Yaxin Liu, Tanye Wen, Jie Fang, Yalong Chen, Ziying Zhou, Xinyi Gu, Hao Wu, Jinghao Sheng, Zhengping Xu, Wei Zou, Baohui Chen
Summary: This study developed a method called "LiveArt" to monitor the activity of RNA polymerase I (Pol I) in real time, using visualization and quantification of endogenous ribosomal RNA (rRNA) synthesis. The findings reveal the mitotic silencing and reactivation of rDNA transcription, as well as the transcriptional kinetics of interphase rDNA. Additionally, the study identifies SRFBP1 as a potential regulator of rRNA synthesis and shows that rDNA transcription occurs in bursts that can be modulated. The importance of this research lies in providing insights into the mechanisms underlying nucleolar functions.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Chemistry, Analytical
Yulong Jin, Kun Xu, Yanyan Huang, Huifei Zhong, Rui Zhao
Summary: The newly developed cell-permeable fluorescent probe can selectively exhibit a ratiometric response towards the apoptotic key enzyme caspase-3, offering potential for real-time quantification of caspase-3 activity to estimate apoptotic stages in living cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Kyeong-Im Hong, Kang Ho Chu, Woo-Dong Jang
Summary: In this study, a series of hydroxy thiophene-conjugated benzothiazole derivatives were prepared and their fluorescence emission behaviors and capability as selective subcellular organelle stains were investigated. The results showed that small structural changes in the derivatives significantly influenced their response to changes in the surrounding environment, resulting in distinct staining patterns in different organelles.
Article
Multidisciplinary Sciences
Hongbao Fang, Shanshan Geng, Mingang Hao, Qixin Chen, Minglun Liu, Chunyan Liu, Zhiqi Tian, Chengjun Wang, Takanori Takebe, Jun-Lin Guan, Yuncong Chen, Zijian Guo, Weijiang He, Jiajie Diao
Summary: The study introduced a new method (Zn-STIMO) for tracking zinc ions in multiple organelles using structured illumination microscopy and a single zinc ion fluorescent probe. By developing a new zinc ion fluorescent probe and combining it with SIM imaging technology, the study successfully achieved morphology-correlated organelle identification in living cells.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Hirohisa Iwaki, Mako Kamiya, Minoru Kawatani, Ryosuke Kojima, Tatsuya Yamasoba, Yasuteru Urano
Summary: This study presents two activatable fluorescence probes for detecting basic carboxypeptidase activity in living cells, which can be used for visualizing cancer tissues. 5ArgAF-FDA showed a stronger intracellular fluorescence increase compared to 5LysAF-FDA, and can differentiate cell lines with varying levels of basic carboxypeptidase activity.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Bochao Chen, Haotong Yuan, Wei Zhang, Jingjing Hu, Xiaoding Lou, Fan Xia
Summary: Organelles play a crucial role in cell function, and their damage and inactivation are closely associated with diseases. Fluorescence molecular tools allow for the observation of organelle activity. AIEgen-peptide bioprobes, which exhibit organelle-targeting ability, have garnered attention due to their biocompatibility, photostability, and diversity. This review summarizes the recent progress in AIEgen-peptide bioprobes targeting organelles such as the cell membrane, nucleus, mitochondria, lysosomes, and endoplasmic reticulum. The structural characteristics, biological applications, and future prospects of these bioprobes are discussed.
Review
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Guoyun Sun, Rodrigo Ledesma-Amaro, Long Liu
Summary: This review presents the pivotal role of microscopy imaging of living cells in metabolic engineering and its applications in industrial microorganisms. It analyzes the advantages and disadvantages of different microscopy technologies and their roles in synthetic biology. Lastly, it discusses the future perspectives of live-cell imaging and its potential in transforming microbial systems.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Robert W. de Gille, Julia M. McCoey, Liam T. Hall, Jean-Philippe Tetienne, E. Pascal Malkemper, David A. Keays, Lloyd C. L. Hollenberg, David A. Simpson
Summary: Research using quantum magnetic imaging techniques to characterize the magnetic properties of cuticulosomes in pigeon cochlea reveals the presence of superparamagnetic and ferrimagnetic domains within individual particles, with magnetic susceptibilities ranging from 0.029 to 0.22. These findings suggest that the physiological roles of cuticulosomes may be related to iron storage and stereocilia stabilization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Mitul Desai, Jitendra Sharma, Adrian L. Slusarczyk, Ashley A. Chapin, Robert Ohlendorf, Agata Wisniowska, Mriganka Sur, Alan Jasanoff
Summary: The study describes a potent approach for noninvasive mapping of cancer-associated enzyme activity using a molecular sensor that acts on the vasculature, providing a diagnostic readout via local changes in hemodynamic image contrast. The optimal FAP sensor variant was identified through screening prototypes and applied for quantitative neuroimaging of FAP activity in rats and nonhuman primates, demonstrating a potentially translatable strategy for ultrasensitive functional imaging of molecular targets in neuromedicine.
Article
Oncology
Guocheng Sun, Shitou Liu, Chaojing Shi, Xi Liu, Qianjin Guo
Summary: This study proposes a method called 3DCNAS to predict subcellular structures of fluorescence using unlabeled transmitted light microscope images. The method leverages deep learning techniques to analyze cell images and extract meaningful information.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bruna Benso, Daniel Bustos, Miguel O. Zarraga, Wendy Gonzalez, Julio Caballero, Sebastian Brauchi
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2019)
Article
Physiology
Lejie Zhang, Mei Zhang, Karl Bellve, Kevin E. Fogarty, Maite A. Castro, Sebastian Brauchi, William R. Kobertz
JOURNAL OF GENERAL PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Lisandra Flores-Aldama, Michael W. Vandewege, Kattina Zavala, Charlotte K. Colenso, Wendy Gonzalez, Sebastian E. Brauchi, Juan C. Opazo
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Laura Bernal, Pamela Sotelo-Hitschfeld, Christine Koenig, Viktor Sinica, Amanda Wyatt, Zoltan Winter, Alexander Hein, Filip Touska, Susanne Reinhardt, Aaron Tragl, Ricardo Kusuda, Philipp Wartenberg, Allen Sclaroff, John D. Pfeifer, Fabien Ectors, Andreas Dahl, Marc Freichel, Viktorie Vlachova, Sebastian Brauchi, Carolina Roza, Ulrich Boehm, David E. Clapham, Jochen K. Lennerz, Katharina Zimmermann
Summary: This study clarifies the molecular and cellular components of the dental cold sensing system, highlighting the role of TRPC5 and TRPA1 in cold sensing in teeth. Odontoblasts are shown to be the direct site of cold transduction. This expands our understanding of the mechanisms behind cold sensitivity in teeth.
Article
Gastroenterology & Hepatology
Gaspar Pena-Munzenmayer, Yusuke Kondo, Constanza Salinas, Jose Sarmiento, Sebastian Brauchi, Marcelo A. Catalan
Summary: This study revealed that Ae4 exchanger activity in secretory salivary gland acinar cells is enhanced upon b-adrenergic receptor stimulation, which can be blocked by H89. Protein sequence analysis identified S173 and S273 as potential targets of cAMP-dependent PKA. Experiments on CHO-K1 cells expressing S173A and S273A mutants showed that S173A, but not S273A, is not activated by PKA.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Biology
Deny Cabezas-Bratesco, Francisco A. Mcgee, Charlotte K. Colenso, Kattina Zavala, Daniele Granata, Vincenzo Carnevale, Juan C. Opazo, Sebastian E. Brauchi
Summary: Transient receptor potential (TRP) proteins are a diverse family of cation-selective channels, with detailed mechanisms of regulation still under debate. Through analysis, a highly conserved set of residues was discovered in TRP channels, positioned at equivalent locations in different subtypes, potentially influencing coupling strategies of various subgroups.
Article
Biochemistry & Molecular Biology
Shu-Hsien Sheu, Srigokul Upadhyayula, Vincent Dupuy, Song Pang, Fei Deng, Jinxia Wan, Deepika Walpita, H. Amalia Pasolli, Justin Houser, Silvia Sanchez-Martinez, Sebastian E. Brauchi, Sambashiva Banala, Melanie Freeman, C. Shan Xu, Tom Kirchhausen, Harald F. Hess, Luke Lavis, Yulong Li, Severine Chaumont-Dubel, David E. Clapham
Summary: This study discovers the existence of synapses between axons and primary cilia and reveals their role in neuronal communication. The cilia are enriched in serotonin receptors and can be stimulated by serotonin released from axons, altering the epigenetic state of the postsynaptic neuron.
Article
Biology
Alexandria N. Miller, Patrick R. Houlihan, Ella Matamala, Deny Cabezas-Bratesco, Gi Young Lee, Ben Cristofori-Armstrong, Tanya L. Dilan, Silvia Sanchez-Martinez, Doreen Matthies, Rui Yan, Zhiheng Yu, Dejian Ren, Sebastian E. Brauchi, David E. Clapham
Summary: This study found that the SARS-CoV-2 and SARS-CoV-1 accessory protein Orf3a colocalizes with markers of various cellular compartments but do not possess functional ion conducting pores. The observed conductances are attributed to contaminants and protein overexpression. Cryo-EM structures reveal a narrow constriction and the presence of a positively charged aqueous vestibule in both Orf3a proteins, which is not conducive to ion permeation. Additionally, SARS-CoV-2 Orf3a interacts with VPS39 and influences the enrichment of the late endosomal marker Rab7, while SARS-CoV-1 Orf3a does not have the same cellular phenotype or interaction with VPS39. The divergent, unstructured loop in SARS-CoV-2 Orf3a facilitates the binding with VPS39 and potentially enables viral exit or host immune evasion through host cellular trafficking mechanisms.
Article
Biochemistry & Molecular Biology
Lisandra Flores-Aldama, Daniel Bustos, Deny Cabezas-Bratesco, Wendy Gonzalez, Sebastian E. Brauchi
Summary: TRPV5 and TRPV6 are calcium-selective ion channels that play a crucial role in maintaining systemic calcium homeostasis. These channels are regulated by intracellular calcium, which induces inactivation. The inactivation of TRPV5 and TRPV6 can be divided into fast and slow phases, with the fast inactivation being unique to TRPV6. By utilizing various techniques, including structural analyses and mutagenesis, we have identified specific amino acids and interactions that govern the inactivation kinetics of mammalian TRPV5 and TRPV6 channels. Additionally, we propose that the association between the intracellular HLH domain and the TRP domain helix contributes to the faster inactivation kinetics observed in mammalian TRPV6 channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Daniel Bustos, Christian Galarza, Wilson Ordonez, Sebastian Brauchi, Bruna Benso
Summary: Transient receptor potential (TRP) channels are important membrane receptors associated with sensory pathways in vertebrates. TRPV1, a widely studied polymodal receptor, is highly sensitive and selective for capsaicin, which can detect heat and pungent compounds. Inhibition of nociceptive transduction through targeting TRPV1 offers a potential approach to pain relief. The development of compounds modulating TRPV1 activity has rapidly expanded, with most drugs interacting near capsaicin's binding site.
Editorial Material
Neurosciences
Sebastian E. Brauchi
Summary: A recent study reveals that two specific residues in the first ankyrin repeat of TRPV1 channels modulate the threshold for temperature activation, emphasizing the importance of considering natural diversity and comparative biology.
TRENDS IN NEUROSCIENCES
(2023)
Article
Multidisciplinary Sciences
Ximena Steinberg, Jason Galpin, Gibran Nasir, Romina Sepulveda, Ernesto Ladron de Guevara, Fernando Gonzalez-Nilo, Leon D. Islas, Christopher A. Ahern, Sebastian E. Brauchi
Article
Cell Biology
Sebastian E. Brauchi, Brad S. Rothberg
Meeting Abstract
Biophysics
Lisandra Flores Aldama, Daniel Bustos, Wendy Gonzalez, Sebastian E. Brauchi
BIOPHYSICAL JOURNAL
(2020)