Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM

A new coronavirus associated with human respiratory disease in China

(2020) Fan Wu et al.   NATURE

A pneumonia outbreak associated with a new coronavirus of probable bat origin

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Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry

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Cheap shots

(2020) Eric Hand   SCIENCE

Structural basis for RNA replication by the SARS-CoV-2 polymerase

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Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

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Stepwise Promoter Melting by Bacterial RNA Polymerase

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Structure of replicating SARS-CoV-2 polymerase

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Structure of V-ATPase from the mammalian brain

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Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir

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Quantifying the heterogeneity of macromolecular machines by mass photometry

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Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex

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Present and Emerging Methodologies in Cryo-EM Single-Particle Analysis

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Challenges and opportunities in cryo-EM single-particle analysis

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Frontiers in Cryo Electron Microscopy of Complex Macromolecular Assemblies

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Delicate structural coordination of the Severe Acute Respiratory Syndrome coronavirus Nsp13 upon ATP hydrolysis

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Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors

(2019) Robert N. Kirchdoerfer et al.   Nature Communications

E. coli TraR allosterically regulates transcription initiation by altering RNA polymerase conformation

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Cryo-electron microscopy for structural analysis of dynamic biological macromolecules

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MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra

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Structure of the core of the type III secretion system export apparatus

(2018) Lucas Kuhlen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quantitative mass imaging of single biological macromolecules

(2018) Gavin Young et al.   SCIENCE

Coronavirus Susceptibility to the Antiviral Remdesivir (GS-5734) Is Mediated by the Viral Polymerase and the Proofreading Exoribonuclease

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Structure of the core of the type III secretion system export apparatus

(2018) Lucas Kuhlen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Transcriptional Responses to ppGpp and DksA

(2018) Richard L. Gourse et al.   Annual Review of Microbiology

Single-particle cryo-EM—How did it get here and where will it go

(2018) Yifan Cheng   SCIENCE

Mechanisms of opening and closing of the bacterial replicative helicase

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6S RNA Mimics B-Form DNA to Regulate Escherichia coli RNA Polymerase

(2017) James Chen et al.   MOLECULAR CELL

TraR directly regulates transcription initiation by mimicking the combined effects of the global regulators DksA and ppGpp

(2017) Saumya Gopalkrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Architecture of eukaryotic mRNA 3′-end processing machinery

(2017) Ana Casañal et al.   SCIENCE

Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state

(2017) Joost Snijder et al.   SCIENCE

Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex

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A Robust Workflow for Native Mass Spectrometric Analysis of Affinity-Isolated Endogenous Protein Assemblies

(2016) Paul Dominic B. Olinares et al.   ANALYTICAL CHEMISTRY

Mass spectrometry guided structural biology

(2016) Idlir Liko et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Native Mass Spectrometry: What is in the Name?

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While the revolution will not be crystallized, biochemistry reigns supreme

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Bayesian Deconvolution of Mass and Ion Mobility Spectra: From Binary Interactions to Polydisperse Ensembles

(2015) Michael T. Marty et al.   ANALYTICAL CHEMISTRY

A Primer to Single-Particle Cryo-Electron Microscopy

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Molecular Basis of Transcription-Coupled Pre-mRNA Capping

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Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity

(2015) Eva Nogales et al.   MOLECULAR CELL

Crystal Structures of the E. coli Transcription Initiation Complexes with a Complete Bubble

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The revolution will not be crystallized: a new method sweeps through structural biology

(2015) Ewen Callaway   NATURE

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

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Structure of a bacterial RNA polymerase holoenzyme open promoter complex

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Continuous and Discontinuous RNA Synthesis in Coronaviruses

(2015) Isabel Sola et al.   Annual Review of Virology

Bacterial Sigma Factors: A Historical, Structural, and Genomic Perspective

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Boundaries of Mass Resolution in Native Mass Spectrometry

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One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

(2014) Lorenzo Subissi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Coliphage HK022 Nun protein inhibits RNA polymerase translocation

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Interactions between RNA polymerase and the "core recognition element" counteract pausing

(2014) I. O. Vvedenskaya et al.   SCIENCE

The Resolution Revolution

(2014) W. Kuhlbrandt   SCIENCE

Optimization of protein purification and characterization using Thermofluor screens

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High-sensitivity Orbitrap mass analysis of intact macromolecular assemblies

(2012) Rebecca J Rose et al.   NATURE METHODS

Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase

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Structural Basis of Transcription Initiation

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Mechanism of Bacterial Transcription Initiation: RNA Polymerase - Promoter Binding, Isomerization to Initiation-Competent Open Complexes, and Initiation of RNA Synthesis

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A novel method for the production ofin vivo-assembled, recombinantEscherichia coliRNA polymerase lacking the α C-terminal domain

(2011) Kelly-Anne Twist et al.   PROTEIN SCIENCE

RNA Polymerase I Contains a TFIIF-Related DNA-Binding Subcomplex

(2010) Sebastian R. Geiger et al.   MOLECULAR CELL

Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13

(2010) Na-Ra Lee et al.   NUCLEIC ACIDS RESEARCH

Allosteric control of Escherichia coli rRNA promoter complexes by DksA

(2009) S. T. Rutherford et al.   GENES & DEVELOPMENT

Native mass spectrometry: a bridge between interactomics and structural biology

(2008) Albert J R Heck   NATURE METHODS