期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 412, 期 5, 页码 754-771出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2011.01.018
关键词
RNA polymerase; Promoter; Sigma factor; Transcription initiation; Intermediate complexes
资金
- National Institutes of Health [GM23467]
Initiation of RNA synthesis from DNA templates by RNA polymerase (RNAP) is a multi-step process, in which initial recognition of promoter DNA by RNAP triggers a series of conformational changes in both RNAP and promoter DNA. The bacterial RNAP functions as a molecular isomerization machine, using binding free energy to remodel the initial recognition complex, placing downstream duplex DNA in the active site cleft and then separating the nontemplate and template strands in the region surrounding the start site of RNA synthesis. In this initial unstable open complex the template strand appears correctly positioned in the active site. Subsequently, the nontemplate strand is repositioned and a clamp is assembled on duplex DNA downstream of the open region to form the highly stable open complex, RPo. The transcription initiation factor, sigma(70), plays critical roles in promoter recognition and RPo formation as well as in early steps of RNA synthesis. (C) 2011 Elsevier Ltd. All rights reserved.
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