N-terminomics – its past and recent advancements

iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform

(2020) Shinyeong Ju et al.   ANALYTICAL CHEMISTRY

Exopeptidase Assisted N- and C-Terminal Proteome Sequencing

(2020) Andreas O. Helbig et al.   ANALYTICAL CHEMISTRY

Analysis and prediction of human acetylation using a cascade classifier based on support vector machine

(2019) Qiao Ning et al.   BMC BIOINFORMATICS

Sensitive determination of proteolytic proteoforms in limited microscale proteome samples

(2019) Samuel SH Weng et al.   MOLECULAR & CELLULAR PROTEOMICS

A rapid and efficient method for N-termini analysis in short-lived proteins

(2019) Lanting Li et al.   TALANTA

‘Shotgun’ proteomic analyses without alkylation of cysteine

(2019) Jacek R. Wiśniewski et al.   ANALYTICA CHIMICA ACTA

Comprehensive Analysis of Protein N-Terminome by Guanidination of Terminal Amines

(2019) Mingwei Sun et al.   ANALYTICAL CHEMISTRY

The benefits (and misfortunes) of SDS in top-down proteomics

(2018) Carolyn Kachuk et al.   Journal of Proteomics

Simple, scalable, and ultrasensitive tip-based identification of protease substrates

(2018) Gerta Shema et al.   MOLECULAR & CELLULAR PROTEOMICS

How many human proteoforms are there?

(2018) Ruedi Aebersold et al.   Nature Chemical Biology

Protein Extraction Methods Shape Much of the Extracted Proteomes

(2018) Liangjie Niu et al.   Frontiers in Plant Science

A Ubiquitous but Overlooked Side Reaction in Dimethyl Labeling of Peptides

(2018) Rijing Liao et al.   ANALYTICAL CHEMISTRY

Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway

(2018) Jeong-Mok Kim et al.   SCIENCE

Monitoring proteolytic processing events by quantitative mass spectrometry

(2017) Mariel Coradin et al.   Expert Review of Proteomics

Enrichment of Cross-Linked Peptides Using Charge-Based Fractional Diagonal Chromatography (ChaFRADIC)

(2017) Verena Tinnefeld et al.   JOURNAL OF PROTEOME RESEARCH

Two proteomic methodologies for defining N-termini of mature human mitochondrial aminoacyl-tRNA synthetases

(2017) Christine Carapito et al.   METHODS

Systematic Evaluation of Protein Reduction and Alkylation Reveals Massive Unspecific Side Effects by Iodine-containing Reagents

(2017) Torsten Müller et al.   MOLECULAR & CELLULAR PROTEOMICS

Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics

(2017) Suttipong Suttapitugsakul et al.   Molecular BioSystems

Engineering peptide ligase specificity by proteomic identification of ligation sites

(2017) Amy M Weeks et al.   Nature Chemical Biology

Targeting the N terminus for site-selective protein modification

(2017) Christian B Rosen et al.   Nature Chemical Biology

The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017 and a comparison with peptidases in the PANTHER database

(2017) Neil D Rawlings et al.   NUCLEIC ACIDS RESEARCH

A rapid and easy protein N-terminal profiling strategy using (N -Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP) labeling and StageTip

(2017) Yanchang Li et al.   PROTEOMICS

Comprehensive analysis of human protein N-termini enables assessment of various protein forms

(2017) Jeonghun Yeom et al.   Scientific Reports

A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin from Clostridium septicum

(2017) Lorena Vázquez-Iglesias et al.   PeerJ

Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis

(2016) Lingfan Chen et al.   ANALYTICAL CHEMISTRY

Progress in Top-Down Proteomics and the Analysis of Proteoforms

(2016) Timothy K. Toby et al.   Annual Review of Analytical Chemistry

Biological significance of co- and post-translational modifications of the yeast 26S proteasome

(2016) Hisashi Hirano et al.   Journal of Proteomics

Analyses of Histone Proteoforms Using Front-end Electron Transfer Dissociation-enabled Orbitrap Instruments

(2016) Lissa C. Anderson et al.   MOLECULAR & CELLULAR PROTEOMICS

Comparative top down proteomics of peripheral blood mononuclear cells from kidney transplant recipients with normal kidney biopsies or acute rejection

(2016) John P. Savaryn et al.   PROTEOMICS

Laser-assisted proteolysis for accelerating and enhancing protein N-termini analysis

(2016) Lanting Li et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Protein Termini and Their Modifications Revealed by Positional Proteomics

(2015) Giada Marino et al.   ACS Chemical Biology

A novel method to isolate protein N-terminal peptides from proteome samples using sulfydryl tagging and gold-nanoparticle-based depletion

(2015) Lanting Li et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry

(2015) E. Scheerlinck et al.   ANALYTICAL BIOCHEMISTRY

N-terminal protein modifications: Bringing back into play the ribosome

(2015) Carmela Giglione et al.   BIOCHIMIE

Protein amino-terminal modifications and proteomic approaches for N-terminal profiling

(2015) Zon W Lai et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Comprehensive Analysis of Low-Molecular-Weight Human Plasma Proteome Using Top-Down Mass Spectrometry

(2015) Dong Huey Cheon et al.   JOURNAL OF PROTEOME RESEARCH

The Human Dental Pulp Proteome and N-Terminome: Levering the Unexplored Potential of Semitryptic Peptides Enriched by TAILS to Identify Missing Proteins in the Human Proteome Project in Underexplored Tissues

(2015) Ulrich Eckhard et al.   JOURNAL OF PROTEOME RESEARCH

Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns

(2015) Andrey Tvardovskiy et al.   MOLECULAR & CELLULAR PROTEOMICS

One-step site-specific modification of native proteins with 2-pyridinecarboxyaldehydes

(2015) James I MacDonald et al.   Nature Chemical Biology

The application of terminomics for the identification of protein start sites and proteoforms in bacteria

(2015) Iain J. Berry et al.   PROTEOMICS

Enrichment of protein N-termini by charge reversal of internal peptides

(2015) Zon W. Lai et al.   PROTEOMICS

An improved workflow for quantitative N-terminal charge-based fractional diagonal chromatography (ChaFRADIC) to study proteolytic events inArabidopsis thaliana

(2015) A. Saskia Venne et al.   PROTEOMICS

N-terminome analysis of the human mitochondrial proteome

(2015) Alvaro Sebastian Vaca Jacome et al.   PROTEOMICS

Isolation of acetylated and free N-terminal peptides from proteomic samples based on tresyl-functionalized microspheres

(2015) Lanting Li et al.   TALANTA

Ultrasensitive proteome analysis using paramagnetic bead technology

(2014) C. S. Hughes et al.   Molecular Systems Biology

Formylation of Amines

(2014) Ciera Gerack et al.   MOLECULES

Label-Free Quantitative Proteomics and N-terminal Analysis of Human Metastatic Lung Cancer Cells

(2014) Hophil Min et al.   MOLECULES AND CELLS

Proteome TopFIND 3.0 with TopFINDer and PathFINDer: database and analysis tools for the association of protein termini to pre- and post-translational events

(2014) Nikolaus Fortelny et al.   NUCLEIC ACIDS RESEARCH

Solid-phase N-terminal peptide enrichment study by optimizing trypsin proteolysis on homoarginine-modified proteins by mass spectrometry

(2014) Saiful M. Chowdhury et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Resin-Assisted Enrichment of N-Terminal Peptides for Characterizing Proteolytic Processing

(2013) Jong-Seo Kim et al.   ANALYTICAL CHEMISTRY

Cascleave 2.0, a new approach for predicting caspase and granzyme cleavage targets

(2013) Mingjun Wang et al.   BIOINFORMATICS

Magnetic Immunoaffinity Enrichment for Selective Capture and MS/MS Analysis of N-Terminal-TMPP-Labeled Peptides

(2013) Céline Bland et al.   JOURNAL OF PROTEOME RESEARCH

An Improved Stable Isotope N-Terminal Labeling Approach with Light/Heavy TMPP To Automate Proteogenomics Data Validation: dN-TOP

(2013) Diego Bertaccini et al.   JOURNAL OF PROTEOME RESEARCH

Novel Highly Sensitive, Specific, and Straightforward Strategy for Comprehensive N-Terminal Proteomics Reveals Unknown Substrates of the Mitochondrial Peptidase Icp55

(2013) A. Saskia Venne et al.   JOURNAL OF PROTEOME RESEARCH

Proteolytic Post-translational Modification of Proteins: Proteomic Tools and Methodology

(2013) Lindsay D. Rogers et al.   MOLECULAR & CELLULAR PROTEOMICS

Proteoform: a single term describing protein complexity

(2013) Lloyd M Smith et al.   NATURE METHODS

Protein carbamylation: In vivo modification or in vitro artefact?

(2013) Laxmikanth Kollipara et al.   PROTEOMICS

Proteasix: A tool for automated and large-scale prediction of proteases involved in naturally occurring peptide generation

(2013) Julie Klein et al.   PROTEOMICS

CLIPPER: an add-on to the Trans-Proteomic Pipeline for the automated analysis of TAILS N-terminomics data

(2012) Ulrich auf dem Keller et al.   BIOLOGICAL CHEMISTRY

Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation

(2012) Hongqiang Qin et al.   CHEMICAL COMMUNICATIONS

Mass spectrometry-based proteomics: existing capabilities and future directions

(2012) Thomas E. Angel et al.   CHEMICAL SOCIETY REVIEWS

Evaluation of the Compact High-Field Orbitrap for Top-Down Proteomics of Human Cells

(2012) Dorothy R. Ahlf et al.   JOURNAL OF PROTEOME RESEARCH

Unbiased Selective Isolation of Protein N-terminal Peptides from Complex Proteome Samples Using Phospho Tagging (PTAG) and TiO2-based Depletion

(2012) Geert P. M. Mommen et al.   MOLECULAR & CELLULAR PROTEOMICS

Comparative Large Scale Characterization of PlantversusMammal Proteins Reveals Similar and IdiosyncraticN-α-Acetylation Features

(2012) Willy V. Bienvenut et al.   MOLECULAR & CELLULAR PROTEOMICS

PROSPER: An Integrated Feature-Based Tool for Predicting Protease Substrate Cleavage Sites

(2012) Jiangning Song et al.   PLoS One

On the Scalability and Requirements of Whole Protein Mass Spectrometry

(2011) Philip D. Compton et al.   ANALYTICAL CHEMISTRY

Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic identification of cleavage sites

(2011) Oliver Schilling et al.   BIOLOGICAL CHEMISTRY

MassSQUIRM

(2011) Lauren P. Blair et al.   Epigenetics

Sequence TTKF↓QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin ofMycoplasma hyopneumoniae

(2011) Daniel R. Bogema et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Intact protein separation by chromatographic and/or electrophoretic techniques for top-down proteomics

(2011) Anna Laura Capriotti et al.   JOURNAL OF CHROMATOGRAPHY A

TopFIND, a knowledgebase linking protein termini with function

(2011) Philipp F Lange et al.   NATURE METHODS

Selecting protein N-terminal peptides by combined fractional diagonal chromatography

(2011) An Staes et al.   Nature Protocols

The N-end rule pathway: emerging functions and molecular principles of substrate recognition

(2011) Shashikanth M. Sriram et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

neXtProt: a knowledge platform for human proteins

(2011) L. Lane et al.   NUCLEIC ACIDS RESEARCH

The N-end rule pathway and regulation by proteolysis

(2011) Alexander Varshavsky   PROTEIN SCIENCE

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment

(2010) Kazuhiro Sonomura et al.   ANALYTICAL BIOCHEMISTRY

Value of Using Multiple Proteases for Large-Scale Mass Spectrometry-Based Proteomics

(2010) Danielle L. Swaney et al.   JOURNAL OF PROTEOME RESEARCH

Parallel Proteomics to Improve Coverage and Confidence in the Partially AnnotatedOryctolagus cuniculusMitochondrial Proteome

(2010) Melanie Y. White et al.   MOLECULAR & CELLULAR PROTEOMICS

Profiling ofN-Acetylated Protein Termini Provides In-depth Insights into the N-terminal Nature of the Proteome

(2010) Andreas O. Helbig et al.   MOLECULAR & CELLULAR PROTEOMICS

Multiplex N-terminome Analysis of MMP-2 and MMP-9 Substrate Degradomes by iTRAQ-TAILS Quantitative Proteomics

(2010) Anna Prudova et al.   MOLECULAR & CELLULAR PROTEOMICS

Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products

(2010) Oded Kleifeld et al.   NATURE BIOTECHNOLOGY

Proteome-wide analysis of protein carboxy termini: C terminomics

(2010) Oliver Schilling et al.   NATURE METHODS

A proteome-scale study on in vivo protein Nα-acetylation using an optimized method

(2010) Xumin Zhang et al.   PROTEOMICS

Efficient and clean charge derivatization of peptides for analysis by mass spectrometry

(2010) Mingrui An et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

A method for terminus proteomics: Selective isolation and labeling of N-terminal peptide from protein through transamination reaction

(2009) Kazuhiro Sonomura et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

A proteomics approach to study in vivo protein Nα-modifications

(2009) Xumin Zhang et al.   Journal of Proteomics

Improved visualization of protein consensus sequences by iceLogo

(2009) Niklaas Colaert et al.   NATURE METHODS

Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics

(2009) Paul J Boersema et al.   Nature Protocols

Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini

(2009) G. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic nanoparticles and MS

(2009) Liyan Zhao et al.   PROTEOMICS

Tags for labeling protein N-termini with subtiligase for proteomics

(2008) Hikari A.I. Yoshihara et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Global Sequencing of Proteolytic Cleavage Sites in Apoptosis by Specific Labeling of Protein N Termini

(2008) Sami Mahrus et al.   CELL

Global Mapping of the Topography and Magnitude of Proteolytic Events in Apoptosis

(2008) Melissa M. Dix et al.   CELL

Ortho-proteogenomics: Multiple proteomes investigation through orthology and a new MS-based protocol

(2008) S. Gallien et al.   GENOME RESEARCH

FT-ICR MS optimization for the analysis of intact proteins

(2008) Aleksey V. Tolmachev et al.   INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

Metadegradomics

(2008) Alain Doucet et al.   MOLECULAR & CELLULAR PROTEOMICS

Improved recovery of proteome-informative, protein N-terminal peptides by combined fractional diagonal chromatography (COFRADIC)

(2008) An Staes et al.   PROTEOMICS