Article
Chemistry, Applied
Peter Sagmeister, Dainis Kaldre, Joerg Sedelmeier, Christian Moessner, Kurt Puntener, Dominique Kummli, Jason D. Williams, C. Oliver Kappe
Summary: Continuous flow processing is an effective method for the formation of 1,5-tetrazoles, achieving high yields with high concentration and short residence time. Two orthogonal PAT methods, NMR and FTIR, were utilized for real-time quantification of starting materials and products, facilitating successful technology transfer and process optimization.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Engineering, Civil
Justin R. Brown, Minghao Li, Alessandro Palermo, Stefano Pampanin, Francesco Sarti, Roger Nokes
Summary: Post-tensioned timber technology can enhance the strength and stiffness of mass timber seismic load resisting systems while providing energy dissipation and re-centering capabilities. This paper presents a study on PT CLT single and double wall systems, examining the effects of different connections and proposing improvements to the analytical prediction models.
ENGINEERING STRUCTURES
(2022)
Article
Chemistry, Analytical
Pamula Sri Sruthi, Sivasamy Balasubramanian, Ponnusamy Senthil Kumar, Ashish Kapoor, Muthamilselvi Ponnuchamy, Meenu Mariam Jacob, Sivaraman Prabhakar
Summary: This study developed a miniaturized paper-based pH detection platform using natural dye extracted from red cabbage. The device's performance at different pH values was quantitatively assessed through digital image analysis, and the a* parameter in the CIEL*a*b* color space model was used for quantifying pH variations. The simplicity of fabrication, eco-friendliness due to the use of paper and natural dye, and suitability for in situ pH measurements make this methodology promising for future chemical sensor technologies.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Debbie van der Burg, Leila Josefsson, Asa Emmer, Cari E. Sanger van de Griend
Summary: The biopharmaceutical market is rapidly growing, and effective monitoring of the biopharmaceutical process is crucial for affordable and reliable therapeutics. Capillary electrophoresis (CE) has proven to be a valuable technique for analyzing various aspects of the process, including product concentration, quality attributes, impurities, and nutrients. CE offers many benefits, such as handling complex matrices, high resolving power, minimal sample preparation, rapid analysis, and low solvent and sample consumption.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Abishek Kasturi, Jorge Gabitto, Costas Tsouris, Radu Custelcean
Summary: This paper investigates CO2 capture from powerplant-generated flue gas via a phase-changing process involving absorption with aqueous amino acids and bicarbonate crystallization with bis-iminoguanidines. The results show that the thermodynamic driving force for glycine regeneration mainly comes from the GBIGH(2)(2+) bicarbonate crystallization step, while the rate-limiting step is the protonation of GBIG. This research provides a fundamental basis for developing an effective carbon capture technology with phase-changing amino acid/guanidine absorbents.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Engineering, Chemical
Jiali Du, Feng Wu, Xiaoxun Ma
Summary: The development of intensification technology for spouted beds is a current research focus, and improving their efficiency by reforming their structure is an effective approach. This paper comprehensively reviews the role of intensification technology in various applications of spouted beds, such as hydrodynamics, drying, desulfurization, pyrolysis, coating, biomass and waste gasification, and biomass drying. The potential problems and challenges in current spouted-bed research are also summarized.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Liangliang Lin, Ziyi Zhang, Yuanping Min
Summary: This paper introduces the unique properties, synergistic advantages, and applications of microfluidic plasma, and prospecting its existing challenges.
GREEN PROCESSING AND SYNTHESIS
(2022)
Article
Biochemical Research Methods
Marko Simic, Christian Neuper, Ulrich Hohenester, Christian Hill
Summary: Manufacturers of nanoparticle-based products rely on detailed information about critical process parameters, but traditional offline characterization techniques lack temporal resolution. To address this, the recently introduced Optofluidic Force Induction (of2i) provides real-time counting with single particle sensitivity and high throughput. This study applies of2i to highly polydisperse and multi-modal particle systems, monitoring evolutionary processes over large time scales and introducing a novel process feedback parameter. Our results demonstrate the versatility of of2i in various applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Cintia Casado, Jose Moreno-SanSegundo, Irene De la Obra, Belen Esteban Garcia, Jose Antonio Sanchez Perez, Javier Marugan
Summary: This study focuses on the mechanistic modelling of wastewater disinfection using controlled solar simulated radiation in the solar photoFenton process for the first time. It was found that iron precipitates forming hydroxides at circumneutral pH significantly affect the optical properties of water, and a mechanism for bacterial inactivation was proposed.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Pharmacology & Pharmacy
Brigitta Nagy, Dorian Laszlo Galata, Attila Farkas, Zsombor Kristof Nagy
Summary: This paper assesses the potential of machine learning techniques in pharmaceutical manufacturing and identifies future directions. By evaluating the application of artificial neural networks in pharmaceutical production, it provides guidance for the implementation of intelligent manufacturing lines in the pharmaceutical industry.
Article
Environmental Sciences
Cheong Sheng Lee, Syamsul Rizal Abd Shukor
Summary: The controllable intensified process has received attention from researchers to achieve desired operation, reducing environmental impact and improving safety and efficiency. This paper proposes the EGPM method for controller design in intensified systems, demonstrating better performance and stability compared to other methods. Simulation results show that the EGPM method provides good setpoint tracking and disturbance rejection, and maintains overall stability and control performance as time delay increases.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Civil
Amir Karimi-Nobandegani, Hamid Valipour
Summary: This study evaluates the effect of web openings on the structural behavior of timber-timber composite beams. Through experimental and analytical methods, the influence of factors such as web opening shape, size, and others on the performance of TTC beams is investigated. A mechanistic model for predicting the load carrying capacity of TTC beams is also proposed.
ENGINEERING STRUCTURES
(2022)
Article
Engineering, Chemical
Shengzhe Jia, Wei Hong, Zhenguo Gao, Jiangkang Wang, Junbo Gong
Summary: This study designs different process intensification trajectories in layer melt crystallization (LMC) to enhance separation efficiency. Parameters like distribution coefficient, interfacial solute distribution factor, and mass transfer coefficient are proposed to investigate the intrinsic correlations between technical variables and target parameters. The first proposed strategy optimizes the operating curve through cooling process regulation and temperature cycling. These techniques eliminate fine crystals, promote crystal growth, and reduce impurity adsorption. The incorporation of stirring intensification technique effectively restricts burst nucleation by carefully designing the stirring parameters. Additionally, a coupling technique combining suspension and LMC is introduced, where crystal seeds from the suspension can flow into the tubular crystallizer and serve as growth sites. This coupling technique demonstrates excellent separation efficiency and significant potential in industrial production. Suggestions for further development of LMC technique are provided.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Setthapong Senarat, Pornsarp Pornsawad, Nutdanai Lertsuphotvanit, Jesper Ostergaard, Thawatchai Phaechamud
Summary: This research focuses on the mathematical modeling and experimental observation of drug release from zein-based in situ forming gels (ISG). The results show that 20% w/w zein-based ISG formulations have nearly complete drug release, while 25% w/w zein-based ISG formulations have lower drug release extents. Additionally, the rate of drug transport and the properties of the solvent play significant roles in drug release.
Article
Mechanics
Anton Kollner
Summary: This study introduces a novel analytical modelling approach for robust and efficient predictions of buckling-driven delamination propagation. The model considers various mechanical couplings and contact modes, significantly enhancing the capabilities of current analytical approaches. The findings help identify governing deformation characteristics of buckling-driven delamination growth and provide insights into damage tolerant design and the improvement of compression after impact (CAI) strength in composite structures.
COMPOSITE STRUCTURES
(2021)
Article
Biotechnology & Applied Microbiology
Michael Martinez, Gareth Mannall, Mari Spitali, Edith L. Norrant, Daniel G. Bracewell
Summary: This study established a micro-mixing model for monoclonal antibody precipitation in continuous tubular reactors, finding that increased micro-mixing led to smaller particle sizes and high antibody yields independent of mixing. Precipitates were easily clarified at small scale, but less so at pilot-scale.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Elizabeth Edwards, Maria Livanos, Anja Krueger, Anne Dell, Stuart M. Haslam, C. Mark Smales, Daniel G. Bracewell
Summary: Glycosylation plays a critical role in the quality and characteristics of therapeutic monoclonal antibodies in biologic manufacturing. Advances have been made in upstream processing, such as mammalian cell line engineering, to improve the predictability of glycosylation. Downstream processing strategies, including the use of novel technologies, are also being explored to control glycosylation. However, more research is needed to establish holistic and consistent manufacturing processes for antibody therapies.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Louisa J. Wilson, Will Lewis, Richard Kucia-Tran, Daniel G. Bracewell
Summary: Improvements in upstream processing have increased antibody productivity, but the recovery of harvest material has become more challenging. Integrating upstream and downstream process development can address product purification issues early in the optimization process, reducing the need for costly downstream clearance strategies. Longer cell culture durations were found to negatively impact product quality, with an increase in post-protein A HCPs and a decrease in product monomer percentage.
BIOTECHNOLOGY PROGRESS
(2022)
Article
Biotechnology & Applied Microbiology
Maria Parau, Thomas F. Johnson, James Pullen, Daniel G. Bracewell
Summary: Titer improvement in mAb manufacture has led to increased cell densities and associated process-related impurities. New depth filter materials offer the potential to remove these impurities at an early stage, but understanding their mechanism of impurity removal is essential.
BIOTECHNOLOGY PROGRESS
(2022)
Article
Biotechnology & Applied Microbiology
Amber R. Peswani, Jaraspim Narkpuk, Anja Krueger, Daniel G. Bracewell, Porntippa Lekcharoensuk, Stuart M. Haslam, Anne Dell, Peera Jaru-Ampornpan, Colin Robinson
Summary: This study presents a new production workflow for an updated and cost-effective PCV2d vaccine candidate, showing high yields of purified PCV2d-based antigen from E. coli fermentation systems and a chimeric PCV2d-PCV3 capsid construct. The recombinant protein effectively induced capsid-specific and PCV2d-neutralizing antibodies in immunized animals, suggesting significant potential as a new vaccine candidate that can be easily manufactured at commercial scale.
FOOD AND BIOPRODUCTS PROCESSING
(2022)
Review
Biochemical Research Methods
Haneen Alosert, James Savery, Jennifer Rheaume, Matthew Cheeks, Richard Turner, Christopher Spencer, Suzanne S. Farid, Stephen Goldrick
Summary: Ensuring data integrity is essential in the biopharmaceutical sector to meet regulatory standards and ensure patient safety. This paper discusses common data integrity violations, links them to regulatory principles, and highlights the role of validated computerized systems in mitigating data integrity risks.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemical Research Methods
Braulio Carrillo Sanchez, Matthew Hinchliffe, Daniel G. Bracewell
Summary: Extracellular vesicles (EVs) are considered as nano-scale molecular messengers due to their ability to deliver bioactive cargos. This study presents the use of GFP-tagged EVs for rapid quantification and monitoring of EVs production, highlighting the utility of this approach for accelerated process development.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemical Research Methods
Benjamin Barnes, Thomas Caws, Samantha Thomas, Alex P. Shephard, Randolph Corteling, Paul Hole, Daniel G. Bracewell
Summary: The purification of extracellular vesicles (EVs) is a major challenge in fundamental research and commercial applications. The study investigates the potential of heparin affinity chromatography (HAC) in purifying neural stem cell-derived EVs. HAC demonstrates high efficiency in removing residual protein and DNA, and it can also separate EVs into different subgroups.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Multidisciplinary Sciences
Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby
Summary: EKL variants with improved activity and solubility were obtained through error-prone PCR and DNA shuffling. Stability was found to be a major factor affecting enzyme activity, with higher melting temperatures enabling better expression. Codon optimization improved enzyme activity, but non-optimized codons and lower expression temperatures resulted in the highest activity due to improved protein quality.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
John M. Hickey, Shaleem I. Jacob, Andrew S. Tait, Fatemeh Dastjerdi Vahid, Joseph Barritt, Sarah Rouse, Alexander Douglas, Sangeeta B. Joshi, David B. Volkin, Daniel G. Bracewell
Summary: Adenovirus vectors have become important vaccines, and electron microscopy and mass spectrometry can be used to analyze virus morphology and proteins, which helps to understand the product heterogeneity and purity, and contributes to the monitoring of viral vaccine quality during the process.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Maria Parau, James Pullen, Daniel G. Bracewell
Summary: Upstream advances in biotechnology have resulted in higher monoclonal antibody (mAb) titers and cell densities in fed-batch cell cultures, but this has also led to increased process-related impurities such as DNA and Host Cell Protein (HCP). Depth filtration is commonly used for harvesting CHO cell cultures, but there is a need to understand its effectiveness in removing impurities. This study investigates the effect of depth filter material and loading on the Protein A resin lifetime and reveals the impact of loading on DNA breakthrough and foulant formation.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biochemical Research Methods
Sushobhan Bandyopadhyay, Vasos Pavlika, Daniel G. Bracewell, Darren N. Nesbeth
Summary: We constructed a three-input biological logic gate, S OR (G XNOR M), to optimize co-expression of two transgenes in Komagataella phaffii using batch-mode carbon source switching. This logic gate functionality enabled use of CSS to accumulate approximately 80% of total virus-like particle (VLP) yield before cells were burdened with lipase expression in 250 mL DasGip bioreactor cultivation.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemical Research Methods
Thomas F. Johnson, Mariachiara Conti, Francesco Iacoviello, Paul R. Shearing, James Pullen, Simone Dimartino, Daniel G. Bracewell
Summary: This study applied X-ray computed tomography to image and characterize 3D-printed gyroids for bioseparation. It visualized structures from the overall geometry down to individual nanopores and achieved digital representations across multiple length scales for geometric analysis and flow simulation. Comparisons between the imaged samples and the original designs were made to analyze printing fidelity, and parameters such as porosity, tortuosity factor, and pore size were measured.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
George Pamenter, Lee Davies, Carol Knevelman, James Miskin, Kyriacos Mitrophanous, Duygu Dikicioglu, Daniel G. Bracewell
Summary: The use of Lentiviral vectors (LVs) in clinical Cell and Gene Therapy applications is increasing, but there is a significant challenge of product loss during capture chromatography. A study demonstrates that the recovery of HIV-1-LV on quaternary-amine membrane adsorbents is dependent on the time in the adsorbed state. Kinetic data shows a rapid drop in functional recovery due to increased irreversible binding, and gradient elution reveals two distinct binding subpopulations.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Medicinal
Thomas B. Fanthom, Christopher Wilson, David Gruber, Daniel G. Bracewell
Summary: Peristaltic pumping during bioprocessing can cause therapeutic protein loss and aggregation, and the root cause of protein loss is the mechanism of solid-solid contact.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
