期刊
FOODS
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/foods9111691
关键词
durum wheat variety; genetic traceability; single nucleotide polymorphisms; colorimetric tests; authenticity; point of care test
资金
- Horizon 2020 INVITE project [817970]
- H2020 Societal Challenges Programme [817970] Funding Source: H2020 Societal Challenges Programme
The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties.
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