Article
Immunology
Dengke Qin, Huihui Xu, Zechuan Chen, Xujing Deng, Shan Jiang, Xiaoming Zhang, Shihua Bao
Summary: Flow cytometry was used to analyze the immune cell composition in the peripheral blood and decidual tissue of women with recurrent pregnancy loss (RPL). The results showed abnormalities in the immune cell profiles of RPL patients, but further verification is needed to establish correlations with previous miscarriages.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Feiyang Wang, Wentong Jia, Mengjie Fan, Xuan Shao, Zhilang Li, Yongjie Liu, Yeling Ma, Yu-Xia Li, Rong Li, Qiang Tu, Yan-Ling Wang
Summary: This study compared the cellular and molecular characteristics of decidual and peripheral leukocytes in normal and unexplained recurrent miscarriage (RM) pregnancies using single-cell RNA sequencing (scRNA-seq). The results revealed significant differences in immune cell subsets and molecular properties in RM cases, providing insights into the immune pathogenesis of early pregnancy loss.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Obstetrics & Gynecology
Shihua Bao, Zechuan Chen, Dengke Qin, Huihui Xu, Xujing Deng, Ruixiu Zhang, Jiaqiang Ma, Zhouping Lu, Shan Jiang, Xiaoming Zhang
Summary: Three distinct subpopulations of decidual stromal cells (DSCs) were identified, with inflammatory and glycolytic subpopulations significantly enriched in recurrent miscarriage (RM), suggesting their roles in driving decidual inflammation and immune dysregulation.
HUMAN REPRODUCTION
(2023)
Article
Biotechnology & Applied Microbiology
Felix J. Hartmann, Dunja Mrdjen, Erin McCaffrey, David R. Glass, Noah F. Greenwald, Anusha Bharadwaj, Zumana Khair, Sanne G. S. Verberk, Alex Baranski, Reema Baskar, William Graf, David Van Valen, Jan Van den Bossche, Michael Angelo, Sean C. Bendall
Summary: This antibody-based method allows profiling of metabolic protein expression and regulation in single cells using mass spectrometry while characterizing cellular phenotype. The approach was validated using mass cytometry and applied to clinical samples, revealing tissue-restricted, metabolically repressed immune cells in human colorectal carcinoma. The combination with multiplexed ion beam imaging unveiled the spatial organization of metabolic programs in human tissues.
NATURE BIOTECHNOLOGY
(2021)
Article
Obstetrics & Gynecology
Ren Hou, Renliang Huang, Yanling Zhou, Dan Lin, Jing Xu, Liuqing Yang, Xiaolan Wei, Zhuoming Xie, Qiaomiao Zhou
Summary: This study aimed to investigate the decidual microenvironment in euploid and aneuploid missed abortions and elective termination of pregnancies. The results showed that the microenvironment of the decidual tissue in the missed-abortion group was altered, with imbalances in stromal cells, natural killer cells, macrophages, and epithelial cells compared to the elective terminations of pregnancy group. Additionally, a correlation was found between the proportion of senescent stromal cells and chromosomal abnormalities in missed-abortion embryos. The findings suggest that targeting dysregulated genes in the decidual tissue and reversing the maternal microenvironment may improve the clinical outcome of missed abortions.
FERTILITY AND STERILITY
(2023)
Article
Biochemistry & Molecular Biology
Fengrun Sun, Liyuan Cui, Jinfeng Qian, Mengdie Li, Lanting Chen, Chunqin Chen, Dajin Li, Songcun Wang, Meirong Du
Summary: Iron is crucial for biological processes, but excessive iron can be toxic. In patients with recurrent pregnancy loss (RPL), low serum iron and iron deposition in the decidua were simultaneously observed. Mice fed with a low-iron diet also showed iron deposition in the decidua and adverse pregnancy outcomes. Decreased expression of ferroportin, an iron exporter, in decidual stromal cells (DSCs) may explain the local iron deposition. Iron supplementation reduced iron deposition and improved pregnancy outcomes. Excessive iron led to ferroptosis of DSCs, but supplementation of glutathione (GSH) and cystine reduced cell death. Ferroptosis inhibitors also attenuated DSC ferroptosis and reversed embryo loss, indicating a potential therapeutic target for RPL patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Meryam Cheloufi, Alaa Kazhalawi, Anne Pinton, Mona Rahmati, Lucie Chevrier, Laura Prat-ellenberg, Anne-Sophie Michel, Geraldine Dray, Arsene Mekinian, Gilles Kayem, Nathalie Ledee
Summary: The study found that personalized strategies based on immune dysregulations, particularly through uterine immune profiling, are associated with higher rates of live births in women with a history of repeated pregnancy loss (RPL). Meanwhile, the use of ART may have a negative impact on live birth rates.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Liling Wang, Huan Wang, Jing Luo, Ting Xie, Gil Mor, Aihua Liao
Summary: The study found that decorin derived from decidual stromal cells can polarize decidual macrophages towards the M1 phenotype by regulating mitochondrial metabolism, resulting in recurrent pregnancy loss.
Article
Cell Biology
Eliza Grbac, Teresa So, Swati Varshney, Nicholas Williamson, Evdokia Dimitriadis, Ellen Menkhorst
Summary: Research suggests that prednisolone affects the expression of cytokines and protein production in human endometrial stromal fibroblasts (hESF), altering their regulation of trophoblast cells. Decidualization of hESF in the presence of prednisolone enhances trophoblast cell growth and influences the expression of cell motility genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Song Han, Dongtao Fu, Gerik W. Tushoski, Lingsong Meng, Kelly M. Herremans, Andrea N. Riner, Thomas J. Geoge, Zhiguang Huo, Steven J. Hughes
Summary: This study explores the impact of tumor microenvironment heterogeneity on patient outcomes in pancreatic ductal adenocarcinoma (PDAC) at the single-cell level using mplxDSP technology. The results suggest that myofibroblasts adjacent to PDAC tumors overexpress certain genes, promoting adaptive immune response. The proximity of cancer-associated fibroblasts and leukocytes to the tumor significantly differs from those farther away, shedding light on the influence of microenvironment on immune tolerance.
Article
Immunology
Xing-Xing Zhen, Long Yang, Yan Gu, Qian Yang, Wen-Wen Gu, Ya-Ping He, Yan-Ling Wang, Jian Wang
Summary: Decidual macrophages (dM phi) play critical roles in maintaining pregnancy at the maternal-fetal interface, and aberrantly increased expression of MNSF beta in dM phi from patients with recurrent pregnancy loss (RPL) may promote TNF alpha production via its interaction with RC3H1, potentially disrupting the immune balance and leading to pregnancy loss.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yu Yan, Guorong Yan, Zhi Cao, Bo Wang, Qingyu Zeng, Lei Shi, Qihang Chang, Chengqian Chen, Linglin Zhang, Caihe Liao, Shengkai Jin, Xiaofei Sun, Guolong Zhang, Peiru Wang, Xiuli Wang
Summary: ALA-PDT improves the immune microenvironment in photoaged skin by enhancing T cell function, reducing the proportion of Langerhans cells, and promoting cell-cell communication among immune cells. These effects last for 6 months and provide an important immunological basis for further exploring strategies to reverse skin aging.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Developmental Biology
Jessica M. Toothaker, Oluwabunmi Olaloye, Blake T. McCourt, Collin C. McCourt, Tatiana N. Silva, Rebecca M. Case, Peng Liu, Dean Yimlamai, George Tseng, Liza Konnikova
Summary: Maintenance of a healthy pregnancy depends on the balance between the fetal and maternal immune systems. This study used advanced techniques to investigate the immune system in mid-gestation human placental villi and found multiple subtypes of fetal immune cells, including memory T cells. It was also discovered that T cells isolated from placental villi had enhanced proliferative capacity compared to adult T cells.
Review
Immunology
Xiuhong Zhang, Haiming Wei
Summary: Pregnancy is a unique immunological process, with NK cells playing a key role in embryonic development and placenta formation. dNK cells are critical in both normal and pathological pregnancies (such as preeclampsia, recurrent spontaneous abortion, endometriosis, and recurrent implantation failure).
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hsien-Ming Wu, Liang-Hsuan Chen, Le-Tien Hsu, Chyong-Huey Lai
Summary: Embryo-endometrial communication plays a critical role in successful embryo implantation and pregnancy establishment through the regulation of maternal immune responses. Recent research has highlighted the importance of extracellular vesicles (EVs) and microRNAs in this process, showing that they modulate maternal immune responses and promote cross-communication between the embryo and the endometrium. Exploring the functions and mechanisms of EVs in embryo implantation and pregnancy may provide valuable insights for reproductive medicine and infertility treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chuang Guo, Bin Li, Huan Ma, Xiaofang Wang, Pengfei Cai, Qiaoni Yu, Lin Zhu, Liying Jin, Chen Jiang, Jingwen Fang, Qian Liu, Dandan Zong, Wen Zhang, Yichen Lu, Kun Li, Xuyuan Gao, Binqing Fu, Lianxin Liu, Xiaoling Ma, Jianping Weng, Haiming Wei, Tengchuan Jin, Jun Lin, Kun Qu
NATURE COMMUNICATIONS
(2020)
Correction
Multidisciplinary Sciences
Qian Liu, Lisa C. Zaba, Ansuman T. Satpathy, Michelle Longmire, Wen Zhang, Kun Li, Jeffrey Granja, Chuang Guo, Jun Lin, Rui Li, Karen Tolentino, Gabriela Kania, Oliver Distler, David Fiorentino, Lorinda Chung, Kun Qu, Howard Y. Chang
NATURE COMMUNICATIONS
(2020)
Article
Immunology
Binqing Fu, Dongyao Wang, Xiaokun Shen, Chuang Guo, Yanyan Liu, Ying Ye, Rui Sun, Jiabin Li, Zhigang Tian, Haiming Wei
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Xianwen Ren, Wen Wen, Xiaoying Fan, Wenhong Hou, Bin Su, Pengfei Cai, Jiesheng Li, Yang Liu, Fei Tang, Fan Zhang, Yu Yang, Jiangping He, Wenji Ma, Jingjing He, Pingping Wang, Qiqi Cao, Fangjin Chen, Yuqing Chen, Xuelian Cheng, Guohong Deng, Xilong Deng, Wenyu Ding, Yingmei Feng, Rui Gan, Chuang Guo, Weiqiang Guo, Shuai He, Chen Jiang, Juanran Liang, Yi-min Li, Jun Lin, Yun Ling, Haofei Liu, Jianwei Liu, Nianping Liu, Shu-Qiang Liu, Meng Luo, Qiang Ma, Qibing Song, Wujianan Sun, GaoXiang Wang, Feng Wang, Ying Wang, Xiaofeng Wen, Qian Wu, Gang Xu, Xiaowei Xie, Xinxin Xiong, Xudong Xing, Hao Xu, Chonghai Yin, Dongdong Yu, Kezhuo Yu, Jin Yuan, Biao Zhang, Peipei Zhang, Tong Zhang, Jincun Zhao, Peidong Zhao, Jianfeng Zhou, Wei Zhou, Sujuan Zhong, Xiaosong Zhong, Shuye Zhang, Lin Zhu, Ping Zhu, Bin Zou, Jiahua Zou, Zengtao Zuo, Fan Bai, Xi Huang, Penghui Zhou, Qinghua Jiang, Zhiwei Huang, Jin-Xin Bei, Lai Wei, Xiu-Wu Bian, Xindong Liu, Tao Cheng, Xiangpan Li, Pingsen Zhao, Fu-Sheng Wang, Hongyang Wang, Bing Su, Zheng Zhang, Kun Qu, Xiaoqun Wang, Jiekai Chen, Ronghua Jin, Zemin Zhang
Summary: The study revealed the presence of immune response dysfunction in COVID-19 patients, with different peripheral immune subtype changes associated with clinical features such as age, sex, severity, and disease stages. Additionally, dramatic transcriptomic changes were observed within virus-infected cells, and upregulation of S100A8/A9 in peripheral blood may contribute to the cytokine storms frequently observed in severe patients.
Article
Biology
Dandan Zong, Beibei Huang, Young Li, Yichen Lu, Nan Xiang, Chuang Guo, Qian Liu, Qing Sha, Pengcheng Du, Qiaoni Yu, Wen Zhang, Pengfei Cai, Yanping Sun, Jinhui Tao, Xiaomei Li, Shanbao Cai, Kun Qu
Summary: This study revealed a CRP-induced pathogenic chromatin dysregulation signature in monocytes from RA patients, suggesting potential therapeutic targets for the disease.
Article
Cell Biology
Nianping Liu, Chen Jiang, Pengfei Cai, Zhuoqiao Shen, Wujianan Sun, Hao Xu, Minghao Fang, Xinfeng Yao, Lin Zhu, Xuyuan Gao, Jingwen Fang, Jun Lin, Chuang Guo, Kun Qu
Summary: The study reveals the inflammatory characteristics of peripheral blood mononuclear cells in COVID-19 patients through single-cell transcriptomic analysis, and identifies shared inflammatory characteristics with HIV infection and sepsis.
Correction
Biochemistry & Molecular Biology
Xianwen Ren, Wen Wen, Xiaoying Fan, Wenhong Hou, Bin Su, Pengfei Cai, Jiesheng Li, Yang Liu, Fei Tang, Fan Zhang, Yu Yang, Jiangping He, Wenji Ma, Jingjing He, Pingping Wang, Qiqi Cao, Fangjin Chen, Yuqing Chen, Xuelian Cheng, Guohong Deng, Xilong Deng, Wenyu Ding, Yingmei Feng, Rui Gan, Chuang Guo, Weiqiang Guo, Shuai He, Chen Jiang, Juanran Liang, Yi-min Li, Jun Lin, Yun Ling, Haofei Liu, Jianwei Liu, Nianping Liu, Shu-Qiang Liu, Meng Luo, Qiang Ma, Qibing Song, Wujianan Sun, GaoXiang Wang, Feng Wang, Ying Wang, Xiaofeng Wen, Qian Wu, Gang Xu, Xiaowei Xie, Xinxin Xiong, Xudong Xing, Hao Xu, Chonghai Yin, Dongdong Yu, Kezhuo Yu, Jin Yuan, Biao Zhang, Peipei Zhang, Tong Zhang, Jincun Zhao, Peidong Zhao, Jianfeng Zhou, Wei Zhou, Sujuan Zhong, Xiaosong Zhong, Shuye Zhang, Lin Zhu, Ping Zhu, Bin Zou, Jiahua Zou, Zengtao Zuo, Fan Bai, Xi Huang, Penghui Zhou, Qinghua Jiang, Zhiwei Huang, Jin-Xin Bei, Lai Wei, Xiu-Wu Bian, Xindong Liu, Tao Cheng, Xiangpan Li, Pingsen Zhao, Fu-Sheng Wang, Hongyang Wang, Bing Su, Zheng Zhang, Kun Qu, Xiaoqun Wang, Jiekai Chen, Ronghua Jin, Zemin Zhang
Article
Biochemical Research Methods
Bin Li, Wen Zhang, Chuang Guo, Hao Xu, Longfei Li, Minghao Fang, Yinlei Hu, Xinye Zhang, Xinfeng Yao, Meifang Tang, Ke Liu, Xuetong Zhao, Jun Lin, Linzhao Cheng, Falai Chen, Tian Xue, Kun Qu
Summary: This study compares 16 integration methods using 45 paired datasets and 32 simulated datasets, finding that Tangram, gimVI, and SpaGE perform best in predicting RNA transcript distribution, while Cell2location, SpatialDWLS, and RCTD are top-performing methods for cell type deconvolution. The benchmark pipeline provided in the study helps researchers in selecting optimal integration methods for their datasets. The comprehensive benchmarking analysis presented in this work evaluates computational methods integrating spatial and single-cell transcriptomics data for transcript distribution prediction and cell type deconvolution.
Article
Genetics & Heredity
Chuang Guo, Mingming Wu, Beibei Huang, Rui Zhao, Linlin Jin, Binqing Fu, Ping Wang, Dongyao Wang, Meijuan Zheng, Jingwen Fang, Haiming Wei, Kun Qu, Fang Ni
Summary: We have identified a unique memory-like NK cell subset (NK2) that accumulates with aging and correlates with disease severity in COVID-19. These memory-like NK2.1 cells exhibit proinflammatory characteristics and a type I interferon response state. Targeting NK2.1 cells may hold potential for developing immunotherapies for infectious diseases and addressing age-related dysfunctions of the immune system.
Article
Cell Biology
Chuang Guo, Qian Liu, Dandan Zong, Wen Zhang, Zuqi Zuo, Qiaoni Yu, Qing Sha, Lin Zhu, Xuyuan Gao, Jingwen Fang, Jinhui Tao, Quan Wu, Xiaomei Li, Kun Qu
Summary: In this study, we investigated the regulome heterogeneities of CD4(+) T cells in SLE patients and healthy controls. We found that the chromatin accessibility signatures of CD4(+) T cells were correlated with disease severity in SLE. Furthermore, we discovered transcriptional dysfunction of regulatory T (Treg) cells in SLE patients, particularly in the CCR7(low)CD74(hi) Treg subgroup which exhibited type I interferon-induced functional exhaustion. These findings provide important insights into the pathogenesis of SLE.
Article
Multidisciplinary Sciences
Qiaoni Yu, Xu Liu, Jingwen Fang, Huihui Wu, Chuang Guo, Wen Zhang, Nianping Liu, Chen Jiang, Qing Sha, Xiao Yuan, Zhikai Wang, Kun Qu
Summary: Although mitotic chromosomes are highly compacted and transcriptionally inert, some active chromatin features are retained during mitosis, referred to as mitotic bookmarking, to ensure proper postmitotic reestablishment of maternal transcriptional programs. Using single-cell transposase-accessible chromatin sequencing (scATAC-seq), this study found that chromatin accessibility changes throughout cell division, with a constant decrease until metaphase and a gradual increase as chromosomes segregate. Certain chromatin regions remain open throughout mitosis, and genes associated with these bookmarked regions are quickly reactivated upon mitotic exit. The nuclear transcription factor Y subunit alpha (NF-YA) plays a role in transcriptional reactivation after mitosis.
Article
Mathematical & Computational Biology
Zhuoqiao Shen, Minghao Fang, Wujianan Sun, Meifang Tang, Nianping Liu, Lin Zhu, Qian Liu, Bin Li, Ruoming Sun, Yu Shi, Chuang Guo, Jun Lin, Kun Qu
Summary: In this study, a user-friendly web database tool called IAAA was developed, which integrates a large amount of autoimmune disease transcriptome data and provides customizable analysis modules and result display formats, offering valuable data resources and convenience for researchers.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2022)
Article
Biochemistry & Molecular Biology
Dongyao Wang, Binqing Fu, Xiaokun Shen, Chuang Guo, Yanyan Liu, Junfei Zhang, Rui Sun, Ying Ye, Jiabin Li, Zhigang Tian, Haiming Wei
Summary: Patients with chronic hepatitis B often do not respond well to IFN-alpha therapy. However, sequential IL-2 therapy has shown efficacy in increasing the frequency and function of HBV-specific CD8(+) T cells, leading to improved clinical outcomes for non-responder CHB patients. This suggests a potential new therapeutic approach for refractory CHB patients.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Proceedings Paper
Biology
Jingwen Fang, Zhaohua Yin, Chuang Guo
2ND INTERNATIONAL CONFERENCE ON FRONTIERS OF BIOLOGICAL SCIENCES AND ENGINEERING (FSBE 2019)
(2020)
