Review
Plant Sciences
Filipe Azevedo-Nogueira, Cecilia Rego, Helena Maria Rodrigues Goncalves, Ana Margarida Fortes, David Gramaje, Paula Martins-Lopes
Summary: This review analyzes the current state of identification and detection of grapevine trunk diseases (GTDs) and emphasizes the urgent need for the development of new technologies. The current methods and protocols are relatively limited and further exploration and research are needed. New detection methods and practices will better control the spread of pathogens and improve vineyard management.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Mariana Patanita, Andre Albuquerque, Maria Doroteia Campos, Patrick Materatski, Carla M. R. Varanda, Joana A. Ribeiro, Maria do Rosario Felix
Summary: Grapevine trunk diseases (GTDs) are complex and challenging to identify and eradicate. This study investigated the fungal community structure associated with GTDs symptomatic and asymptomatic grapevines. The results showed that symptomatic plants had a lower abundance but higher diversity of GTDs-associated fungi. The composition of fungal endophytic communities was significantly different according to the symptomatology and not affected by the cultivar.
Article
Mycology
Y. Y. Zhou, W. Zhang, L. N. Wu, J. Zhang, H. Y. Tan, K. W. T. Chethana, I. S. Manawasinghe, M. Liu, X. H. Li, J. Y. Yan
Summary: This study investigated the diversity of fungal species in grapevine trunk disease samples in China and discovered several species that were reported for the first time in China and worldwide on grapevine. The results provide important insights for further understanding the interactions among fungal communities and strategies for managing grapevine trunk diseases.
Review
Microbiology
Maria-Doinita Muntean, Ana-Maria Dragulinescu, Liliana Lucia Tomoiaga, Maria Comsa, Horia-Silviu Racoare, Alexandra Doina Sirbu, Veronica Sanda Chedea
Summary: Grapevine trunk diseases (GTDs) have become a concern in wine producing countries, including Romania. This study reviews the scientific information on GTDs in Romania and finds that there is limited literature available on the subject. Three out of six fungal GTDs are well documented in Romanian viticultural zones, with management measures focusing on prevention and removal of affected grapevines.
Article
Plant Sciences
Juan L. Chacon-Vozmediano, David Gramaje, Maela Leon, Josep Armengol, Juan Moral, Pedro M. Izquierdo-Canas, Jesus Martinez-Gascuena
Summary: This study aimed to identify tolerant grapevine cultivars to GTD fungi based on external symptoms observed in the vineyard and to characterize the pathogenic mycoflora associated with symptomatic vines. Results showed that some grapevine cultivars displayed high susceptibility to GTDs while others were considered tolerant. The prevalent fungal species isolated from symptomatic vines were Phaeomoniella chlamydospora, Cryptovalsa ampelina, and Dothiorella sarmentorum.
Article
Genetics & Heredity
Bekris Fotios, Vasileiadis Sotirios, Papadopoulou Elena, Samaras Anastasios, Testempasis Stefanos, Gkizi Danae, Tavlaki Georgia, Tzima Aliki, Paplomatas Epaminondas, Markakis Emmanuel, Karaoglanidis George, Papadopoulou K. Kalliope, Karpouzas G. Dimitrios
Summary: The study revealed that grapevine trunk disease symptomatic plants support a wood fungal microbiome, showing cultivar and biogeography-dependent patterns that can be used as a proxy to distinguish between healthy and diseased vines. Furthermore, it highlighted strong interactions between the bacterial and fungal wood microbiome in asymptomatic vines.
ENVIRONMENTAL MICROBIOME
(2021)
Article
Microbiology
Asri Peni Wulandari, Erin Triani, Kartika Sari, Mila Prasetyani, Mohamad Nurzaman, Rully Dyah Purwati, Riksfardini A. Ermawar, Anne Nuraini
Summary: This study demonstrates the potential antifungal activity of endophytic fungi from ramie against plant pathogens. The Penicillium citrinum isolate MEBP0017 showed the highest inhibition against all the ramie pathogens.
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study demonstrates that plant endophytes play a significant role in stimulating stilbene production in grape cell suspension, with endophytic fungi showing a more pronounced induction effect on stilbene accumulation compared to endophytic bacteria. The increase in stilbene content was due to the activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. The findings suggest that grape endophytes could potentially serve as stimulators for stilbene biosynthesis in grapevine or grape cell cultures.
Review
Plant Sciences
Rebeca Cobos, Ana Ibanez, Alba Diez-Galan, Carla Calvo-Pena, Seyedehtannaz Ghoreshizadeh, Juan Jose R. Coque
Summary: This article reviews the recent advances in the study of grapevine microbiome, with a focus on the composition of microbial populations and etiology of plants affected by grapevine trunk diseases (GTDs). It also discusses the potential of grapevine microbiome as a source for new and more promising biocontrol agents (BCAs) for GTD treatment.
Article
Biochemistry & Molecular Biology
Laura Martin, Blanca Garcia-Garcia, Maria del Mar Alguacil
Summary: Esca is a complex and devastating Grapevine Trunk Disease caused by various fungal pathogens. Our study found that Phaeomoniella chlamydospora was the most abundant pathogen, and Aureobasidiaceae could be a potential biocontrol agent for Esca.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Marcelo Bustamante, Karina Elfar, Akif Eskalen
Summary: In this study, a large number of bacteria with inhibitory effects on grapevine trunk diseases were isolated from commercial vineyards in California. These bacteria were identified as Bacillus velezensis, Pseudomonas spp., and Serratia plymuthica, and they showed inhibition against various fungal pathogens in vitro. The results suggest that these bacteria may serve as potential biocontrol agents against grapevine trunk diseases.
Article
Plant Sciences
Loukas Kanetis, Demetris Taliadoros, Georgios Makris, Michalis Christoforou
Summary: This study described four species in the Sporocadaceae family, including two species in the genus Seimatosporium and two species in Sporocadus. Pathogenicity trials showed that Sporocadus isolates were more aggressive than Seimatosporium. Additionally, a newly described species and two species reported for the first time in Europe on Vitis vinifera were found, suggesting the potential importance of Sporocadaceae in grapevine trunk disease complex.
Article
Microbiology
Alejandro Moreno-Gavira, Fernando Dianez, Brenda Sanchez-Montesinos, Mila Santos
Summary: Research showed that Paecilomyces variotii has strong control effects on common phytopathogens in various crops, and it is compatible with most commercial fungicides.
Article
Genetics & Heredity
Yonghua Li, Xinghong Li, Wei Zhang, Jiao Zhang, Hui Wang, Junbo Peng, Xuncheng Wang, Jiye Yan
Summary: This study investigated the association between belowground fungal communities and grapevine trunk disease (GTD). The diversity and composition of the fungal community varied across different soil-plant compartments and sampling years. The presence of GTD symptoms was related to the enrichment of Fusarium spp., rather than the relative abundances of GTD pathogens. These findings provide new insights into the pathogenesis and potential control practices of GTDs.
ENVIRONMENTAL MICROBIOME
(2023)
Review
Microbiology
Marie Belair, Alexia Laura Grau, Julie Chong, Xubin Tian, Jiaxin Luo, Xin Guan, Flora Pensec
Summary: Botryosphaeriaceae is a family of fungi that cause decay in woody plants, particularly in viticulture. Advances in gene studies have helped us understand the pathogenicity mechanisms of these fungi, which can lead to the development of better control methods and higher grape yields.
Article
Plant Sciences
Luis Orduna, Miaomiao Li, David Navarro-Paya, Chen Zhang, Antonio Santiago, Pablo Romero, Ziva Ramsak, Gabriele Magon, Janine Holl, Patrick Merz, Kristina Gruden, Alessandro Vannozzi, Dario Cantu, Jochen Bogs, Darren C. J. Wong, Shao-Shan Carol Huang, Jose Tomas Matus
Summary: The stilbenoid pathway in grapevine was studied, and MYB14 and MYB15 were identified as regulators. The combination of DAP-Seq and GCNs helped determine the target genes of these regulators. The study also revealed that these regulators bind to multiple genes involved in the stilbenoid pathway and directly regulate various metabolic steps, leading to an increase in stilbenoid production.
Article
Biotechnology & Applied Microbiology
Simone Maestri, Giorgio Gambino, Giulia Lopatriello, Andrea Minio, Irene Perrone, Emanuela Cosentino, Barbara Giovannone, Luca Marcolungo, Massimiliano Alfano, Stephane Rombauts, Dario Cantu, Marzia Rossato, Massimo Delledonne, Luciano Calderon
Summary: The study utilized a multi-platform approach to assemble the genome of 'Nebbiolo' clones and analyze structural variations. The results highlight the variations in accumulation rate and functional implications of SVs in the 'Nebbiolo' genome at different organizational levels.
Article
Plant Sciences
Luca Nerva, Jadran F. Garcia, Francesco Favaretto, Gaetano Giudice, Loredana Moffa, Marco Sandrini, Dario Cantu, Alessandro Zanzotto, Massimo Gardiman, Riccardo Velasco, Giorgio Gambino, Walter Chitarra
Summary: This study investigates the influence of host health on the taxa profile and behavior of microbial communities associated with the wood of 20-year-old grapevine plants through metatranscriptomics. The results show that this approach can better define the microbial community and the use of total RNA enables the identification of bacteria with potential biocontrol activities against wood-degrading fungi. Additionally, a large number of new viral species associated with a single environment and with potential impact on the entire holobiont were discovered.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Genetics & Heredity
Aruna Varanasi, Margaret Worthington, Lacy Nelson, Autumn Brown, T. Mason Chizk, Renee Threlfall, Luke Howard, Patrick Conner, Rosa Figueroa-Balderas, Melanie Massonnet, Dario Cantu, John R. Clark
Summary: A study found that the bronze phenotype in muscadine grapes is regulated by the glutathione S-transferase4 gene, which is different from the MybA gene cluster responsible for berry color variation in Vitis vinifera.
G3-GENES GENOMES GENETICS
(2022)
Article
Genetics & Heredity
Andrea Minio, Noe Cochetel, Amanda M. Vondras, Melanie Massonnet, Dario Cantu
Summary: De novo genome assembly is crucial for genomic research, but high-quality assemblies often contain errors due to repeats, heterozygosity, or chosen assembly strategies. This study presents HaploSync, a suite of tools that produces fully phased, chromosome-scale diploid genome assemblies and performs extensive quality control, improving assembly accuracy and completeness.
G3-GENES GENOMES GENETICS
(2022)
Article
Genetics & Heredity
Melanie Massonnet, Amanda M. Vondras, Noe Cochetel, Summaira Riaz, Daniel Pap, Andrea Minio, Rosa Figueroa-Balderas, M. Andrew Walker, Dario Cantu
Summary: This study identified candidate resistance genes associated with grape powdery mildew resistance through haplotype analysis and found that heterozygous structural variations can affect the gene content and domain composition of nucleotide-binding leucine-rich repeat proteins.
G3-GENES GENOMES GENETICS
(2022)
Article
Plant Sciences
Christian Silva, Jaclyn A. Adaskaveg, Saskia D. Mesquida-Pesci, Isabel B. Ortega-Salazar, Sivakumar Pattathil, Lisha Zhang, Michael G. Hahn, Jan A. L. van Kan, Dario Cantu, Ann L. T. Powell, Barbara Blanco-Ulate
Summary: Postharvest fungal pathogen Botrytis cinerea actively induces ripening in tomato fruit to facilitate infection, depending on the presence of two fungal polygalacturonases. It accelerates external coloration, ethylene production, and softening in unripe fruit while upregulating host genes involved in ripening processes. Glycomic analysis also showed similar changes in cell wall polysaccharides between infected unripe fruit and ripe healthy fruit. The failure to accelerate ripening severely inhibits fungal survival on unripe fruit.
Article
Multidisciplinary Sciences
Andrea Minio, Noe Cochetel, Melanie Massonnet, Rosa Figueroa-Balderas, Dario Cantu
Summary: In this study, the genomes of three grape rootstocks derived from wild grape species were successfully assembled, providing valuable resources for studying the genetic basis of grape adaptation to stress and designing markers for rootstock breeding programs.
Article
Genetics & Heredity
Edwin Solares, Abraham Morales-Cruz, Rosa Figueroa Balderas, Eric Focht, Vanessa E. T. M. Ashworth, Skylar Wyant, Andrea Minio, Dario Cantu, Mary Lu Arpaia, Brandon S. Gaut
Summary: The domestication history and population genomics of the avocado were investigated using a reference genome created from a Gwen varietal. The study revealed three separate domestication events and identified potential candidate genes involved in fruit development and floral development. The findings contribute to our understanding of avocado domestication and provide valuable insights into the genetic basis of important traits in this crop.
G3-GENES GENOMES GENETICS
(2022)
Article
Plant Sciences
Melanie Massonnet, Summaira Riaz, Daniel Pap, Rosa Figueroa-Balderas, M. Andrew Walker, Dario Cantu
Summary: This study compared the defense mechanisms associated with different powdery mildew resistance loci in grapes, showing that these loci confer resistance by limiting infection at different stages. The defense mechanisms triggered by powdery mildew were found to be primarily associated with early transcriptional reprogramming. There was limited overlap of defense-related genes between different loci, with nearly half of the genes being specific to a single locus.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Plant Sciences
Michele Perazzolli, Aziz Aziz, David Gramaje, Ana Margarida Fortes, Dario Cantu
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Claudia Castro, Melanie Massonnet, Nancy Her, Biagio DiSalvo, Barbara Jablonska, Daniel R. Jeske, Dario Cantu, M. Caroline Roper
Summary: Priming is an adaptive mechanism in plants that enhances defense responses to pathogens. The lipopolysaccharide (LPS) MAMP isolated from the pathogenic bacterium Xylella fastidiosa acts as a priming stimulus in grapevines, reducing disease symptoms. Gene expression analysis revealed reprogramming and increased expression of defense-related genes in primed vines.
Article
Genetics & Heredity
Andrea Minio, Rosa Figueroa-Balderas, Stephen P. Cohen, Shahin S. Ali, Denny Carriel, Dahyana Britto, Conrad Stack, Indrani K. Baruah, Jean-Philippe Marelli, Dario Cantu, Bryan A. Bailey
Summary: By using long-read sequencing technology, we obtained 5 high-quality M. roreri genomes and discovered inversions, translocations, and duplications in the genomes. We also identified 3 new A locus alleles and 1 new B locus allele. Isolates at the expanding range tend to share lineage-specific structural variants. M. roreri in Costa Rica carries a chromosome segment duplication that includes secreted proteins and candidate effectors.
G3-GENES GENOMES GENETICS
(2023)
Article
Biology
Abraham Morales-Cruz, Jonas Aguirre-Liguori, Melanie Massonnet, Andrea Minio, Mirella Zaccheo, Noe Cochetel, Andrew Walker, Summaira Riaz, Yongfeng Zhou, Dario Cantu, Brandon S. Gaut
Summary: This study investigates the genes contributing to plant resistance against Xylella fastidiosa in wild grapevine species. The researchers identify candidate resistance genes using genome-wide association and find that resistance has evolved multiple times. They also observe that resistance is climate-dependent, with individuals from colder and wetter environments being more susceptible to infection. Climate is shown to be as effective a predictor of resistance phenotypes as genetic markers. The study predicts increased pathogen pressure on grapevines and almonds due to climate change.
COMMUNICATIONS BIOLOGY
(2023)
Article
Plant Sciences
Cheng Zou, Surya Sapkota, Rosa Figueroa-Balderas, Jeff Glaubitz, Dario Cantu, Brewster F. Kingham, Qi Sun, Lance Cadle-Davidson
Summary: Fine mapping of quantitative trait loci (QTL) is essential in modern breeding practice. This study presents a multitiered haplotypic marker system that improves fine mapping accuracy and successfully identifies a grapevine downy mildew resistance locus. The strategy combines different resolutions of genome sequencing and integrates high-density genetic information to pinpoint the genetic basis of QTLs.
