Baculovirus-free insect cell expression system for high yield antibody and antigen production

Human antibodies neutralizing diphtheria toxin in vitro and in vivo

(2020) Esther Veronika Wenzel et al.   Scientific Reports

Directed evolution of super-secreted variants from phage-displayed human Interleukin-2

(2019) Gertrudis Rojas et al.   Scientific Reports

Identifying parameters to improve the reproducibility of transient gene expression in High Five cells

(2019) Maren Bleckmann et al.   PLoS One

How introns enhance gene expression

(2017) Orit Shaul   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Efficient production of antibody Fab fragment by transient gene expression in insect cells

(2017) Keita Mori et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen

(2016) Maren Bleckmann et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells

(2016) Maren Bleckmann et al.   PLoS One

A simple plasmid-based transient gene expression method using High Five cells

(2015) Xiao Shen et al.   JOURNAL OF BIOTECHNOLOGY

Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda

(2015) Maren Bleckmann et al.   PLoS One

The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems

(2015) Tamer Z. Salem et al.   PLoS One

Thirty years of baculovirus-insect cell protein expression: from dark horse to mainstream technology

(2014) M. M. van Oers et al.   JOURNAL OF GENERAL VIROLOGY

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

(2013) Volker Jäger et al.   BMC BIOTECHNOLOGY

Utility of temporally distinct baculovirus promoters for constitutive and baculovirus-inducible transgene expression in transformed insect cells

(2013) Chi-Hung Lin et al.   JOURNAL OF BIOTECHNOLOGY

Virus-free transient protein production in Sf9 cells

(2013) Xiao Shen et al.   JOURNAL OF BIOTECHNOLOGY

A simple, robust and highly efficient transient expression system for producing antibodies

(2013) Tom Vink et al.   METHODS

Toward system-level understanding of baculovirus–host cell interactions: from molecular fundamental studies to large-scale proteomics approaches

(2012) Francisca Monteiro et al.   Frontiers in Microbiology

Optimizing the baculovirus expression vector system

(2011) Richard B. Hitchman et al.   METHODS

Industrialization of mAb production technology: The bioprocessing industry at a crossroads

(2010) Brian Kelley   mAbs