Bioconjugates – From a specialized past to a diverse future

Toward Next-Generation Biohybrid Catalyst Design: Influence of Degree of Polymerization on Enzyme Activity

(2020) Marina Kovaliov et al.   BIOCONJUGATE CHEMISTRY

Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

(2020) Alexis Theodorou et al.   Nature Communications

Proteins Conjugated with Sulfoxide-Containing Polymers Show Reduced Macrophage Cellular Uptake and Improved Pharmacokinetics

(2020) Ye Yu et al.   ACS Macro Letters

Molecular Sieving on the Surface of a Nano-Armored Protein

(2019) Bibifatima Kaupbayeva et al.   BIOMACROMOLECULES

Pharmacokinetics, excretion, distribution, and metabolism of 60-kDa polyethylene glycol used in BAY 94-9027 in rats and its value for human prediction

(2019) Andreas Baumann et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

In Situ Growth of a Cationic Polymer from the N-Terminus of Glucose Oxidase To Regulate H2O2 Generation for Cancer Starvation and H2O2 Therapy

(2019) Hanjun Hao et al.   ACS Applied Materials & Interfaces

Protein PEPylation: A New Paradigm of Protein–Polymer Conjugation

(2019) Yingqin Hou et al.   BIOCONJUGATE CHEMISTRY

Effect of Polymer Chain Density on Protein–Polymer Conjugate Conformation

(2019) Daniela Russo et al.   BIOMACROMOLECULES

A charge-maintaining ATRP initiator rescues the lost function of negatively charged protein-polymer conjugates

(2019) Stefanie L Baker et al.   BIOMACROMOLECULES

Structure determination using solution NMR: Is it worth the effort?

(2019) Koh Takeuchi et al.   JOURNAL OF MAGNETIC RESONANCE

Layered Co-Immobilization of β-Glucosidase and Cellulase on Polymer Film by Visible-Light-Induced Graft Polymerization

(2019) Yindian Wang et al.   ACS Applied Materials & Interfaces

Transforming protein-polymer conjugate purification by tuning protein solubility

(2019) Stefanie L. Baker et al.   Nature Communications

Modification of Lipase with Poly(4-acryloylmorpholine) Enhances Solubility and Transesterification Activity in Anhydrous Ionic Liquids

(2018) Garrett R. Chado et al.   BIOMACROMOLECULES

Grafting through Method for Implanting of Lysozyme Enzyme in Molecular Brush for Improved Biocatalytic Activity and Thermal Stability

(2018) Xue Wang et al.   MACROMOLECULES

Synthesis of lipase polymer hybrids with retained or enhanced activity using the grafting-from strategy

(2018) Marina Kovaliov et al.   POLYMER

Solid-phase synthesis of protein-polymers on reversible immobilization supports

(2018) Hironobu Murata et al.   Nature Communications

Intramolecular Interactions of Conjugated Polymers Mimic Molecular Chaperones to Stabilize Protein–Polymer Conjugates

(2018) Stefanie L. Baker et al.   BIOMACROMOLECULES

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation

(2018) Josefine Morgenstern et al.   BIOMACROMOLECULES

Azlactone-based heterobifunctional linkers with orthogonal clickable groups: efficient tools for bioconjugation with complete atom economy

(2018) Hien The Ho et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Grafting-from lipase: utilization of a common amino acid residue as a new grafting site

(2018) Marina Kovaliov et al.   Polymer Chemistry

PEG Analogs Synthesized by Ring-Opening Metathesis Polymerization for Reversible Bioconjugation

(2018) Emma M. Pelegri-O’Day et al.   BIOCONJUGATE CHEMISTRY

Site-Selective in Situ Growth-Induced Self-Assembly of Protein–Polymer Conjugates into pH-Responsive Micelles for Tumor Microenvironment Triggered Fluorescence Imaging

(2018) Pengyong Li et al.   BIOMACROMOLECULES

Utilizing functionalized bromomaleimides for fluorogenic conjugation and PEGylation of enzymes

(2018) Jonathan T Husband et al.   POLYMER INTERNATIONAL

Exploiting the Benefits of Homogeneous and Heterogeneous Biocatalysis: Tuning the Molecular Interaction of Enzymes with Solvents via Polymer Modification

(2018) Garrett R. Chado et al.   ACS Catalysis

Probing Membrane Hydration at the Interface of Self-Assembled Peptide Amphiphiles Using Electron Paramagnetic Resonance

(2018) Ian R. Smith et al.   ACS Macro Letters

Effect of PEG molecular weight and PEGylation degree on the physical stability of PEGylated lysozyme

(2017) Josefine Morgenstern et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Polymer Structure and Conformation Alter the Antigenicity of Virus-like Particle–Polymer Conjugates

(2017) Parker W. Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photoinduced Oxygen Reduction for Dark Polymerization

(2017) Sivaprakash Shanmugam et al.   MACROMOLECULES

Enzyme-Assisted Photoinitiated Polymerization-Induced Self-Assembly: An Oxygen-Tolerant Method for Preparing Block Copolymer Nano-Objects in Open Vessels and Multiwell Plates

(2017) Jianbo Tan et al.   MACROMOLECULES

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

(2017) Jia Niu et al.   Nature Chemistry

Oxygen tolerant photopolymerization for ultralow volumes

(2017) Jonathan Yeow et al.   Polymer Chemistry

Tyrosine-Triazolinedione Bioconjugation as Site-Selective Protein Modification Starting from RAFT-Derived Polymers

(2017) Stef Vandewalle et al.   ACS Macro Letters

Grafting-From Proteins Using Metal-Free PET–RAFT Polymerizations under Mild Visible-Light Irradiation

(2017) Bryan S. Tucker et al.   ACS Macro Letters

Tertiary Structure-Based Prediction of How ATRP Initiators React with Proteins

(2017) Sheiliza Carmali et al.   ACS Biomaterials-Science & Engineering

Tertiary Structure-Based Prediction of How ATRP Initiators React with Proteins

(2017) Sheiliza Carmali et al.   ACS Biomaterials Science & Engineering

Investigating the Impact of Polymer Functional Groups on the Stability and Activity of Lysozyme–Polymer Conjugates

(2016) Melissa Lucius et al.   BIOMACROMOLECULES

Quantification of PEGylated proteases with varying degree of conjugation in mixtures: An analytical protocol combining protein precipitation and capillary gel electrophoresis

(2016) Josefine Morgenstern et al.   JOURNAL OF CHROMATOGRAPHY A

Aqueous RAFT Photopolymerization with Oxygen Tolerance

(2016) Sivaprakash Shanmugam et al.   MACROMOLECULES

Polymer–protein hybrid scaffolds as carriers for CORM-3: platforms for the delivery of carbon monoxide (CO)

(2016) Diep Nguyen et al.   RSC Advances

Self-Assembly of Temperature-Responsive Protein–Polymer Bioconjugates

(2015) Dafni Moatsou et al.   BIOCONJUGATE CHEMISTRY

The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

(2015) Rebecca Falatach et al.   CHEMICAL COMMUNICATIONS

Structure of a PEGylated protein reveals a highly porous double-helical assembly

(2015) Giada Cattani et al.   Nature Chemistry

PEGylated Biopharmaceuticals

(2015) Inge A. Ivens et al.   TOXICOLOGIC PATHOLOGY

Protein ROMP: Aqueous Graft-from Ring-Opening Metathesis Polymerization

(2015) Sergey A. Isarov et al.   ACS Macro Letters

Cooperative, Reversible Self-Assembly of Covalently Pre-Linked Proteins into Giant Fibrous Structures

(2014) Saadyah Averick et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bridging Disulfides for Stable and Defined Antibody Drug Conjugates

(2014) George Badescu et al.   BIOCONJUGATE CHEMISTRY

Pharmacokinetics, metabolism and distribution of PEGs and PEGylated proteins: quo vadis?

(2014) Andreas Baumann et al.   DRUG DISCOVERY TODAY

Therapeutic Protein–Polymer Conjugates: Advancing Beyond PEGylation

(2014) Emma M. Pelegri-O’Day et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Thermoresponsive Self-Assembly of Nanostructures from a Collagen-Like Peptide-Containing Diblock Copolymer

(2014) Tianzhi Luo et al.   MACROMOLECULAR BIOSCIENCE

Reprogramming the specificity of sortase enzymes

(2014) Brent M. Dorr et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polymer-Based Protein Engineering Can Rationally Tune Enzyme Activity, pH-Dependence, and Stability

(2013) Hironobu Murata et al.   BIOMACROMOLECULES

Tailoring enzyme activity and stability using polymer-based protein engineering

(2013) Chad Cummings et al.   BIOMATERIALS

Sugar overcomes oxygen inhibition in photoinitiated free radical polymerization

(2013) Faruk Oytun et al.   JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

Molecular Sieving on the Surface of a Protein Provides Protection Without Loss of Activity

(2012) Mi Liu et al.   ADVANCED FUNCTIONAL MATERIALS

Highly efficient disulfide bridging polymers for bioconjugates from radical-compatible dithiophenol maleimides

(2012) Mathew W. Jones et al.   CHEMICAL COMMUNICATIONS

Modification of N-Terminal α-Amino Groups of Peptides and Proteins Using Ketenes

(2012) Anna On-Yee Chan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Protein–Polymer Hybrid Mediated By DNA

(2012) Saadyah E. Averick et al.   LANGMUIR

The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone

(2011) Sheetal S. Pai et al.   BIOCONJUGATE CHEMISTRY

Polymer therapeutics as nanomedicines: new perspectives

(2011) Ruth Duncan   CURRENT OPINION IN BIOTECHNOLOGY

Block copolymer conjugates prepared by sequentially grafting from proteins via RAFT

(2011) Hongmei Li et al.   Polymer Chemistry

FDA-approved poly(ethylene glycol)–protein conjugate drugs

(2011) Steevens N. S. Alconcel et al.   Polymer Chemistry

Toward the Fourth Dimension of Membrane Protein Structure: Insight into Dynamics from Spin-Labeling EPR Spectroscopy

(2011) Hassane S. Mchaourab et al.   STRUCTURE

ATRP under Biologically Relevant Conditions: Grafting from a Protein

(2011) Saadyah Averick et al.   ACS Macro Letters

Effect of hydrophilic polymer conjugation on heat-induced conformational changes in a protein

(2010) Ji-Hun Seo et al.   Acta Biomaterialia

In Situ Growth of Side-Chain PEG Polymers from Functionalized Human Growth Hormone—A New Technique for Preparation of Enhanced Protein−Polymer Conjugates

(2010) Johannes Pall Magnusson et al.   BIOCONJUGATE CHEMISTRY

Arginine-Specific Modification of Proteins with Polyethylene Glycol

(2010) Marc A. Gauthier et al.   BIOMACROMOLECULES

Biochemical and biophysical characterization of lysozyme modified by PEGylation

(2010) Débora da Silva Freitas et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Genetically Encoded Initiator for Polymer Growth from Proteins

(2010) Jennifer C. Peeler et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Direct evidence for a two-state protein unfolding transition from hydrogen-deuterium exchange, mass spectrometry, and NMR

(2010) Qian Yi et al.   PROTEIN SCIENCE

Monitoring Protein−Polymer Conjugation by a Fluorogenic Cu(I)-Catalyzed Azide−Alkyne 1,3-Dipolar Cycloaddition

(2009) A. (Ton) J. Dirks et al.   BIOCONJUGATE CHEMISTRY

Analytical and preparative separation of PEGylated lysozyme for the characterization of chromatography media

(2009) Anna Moosmann et al.   JOURNAL OF CHROMATOGRAPHY A

Trapping of Thiol-Terminated Acrylate Polymers with Divinyl Sulfone To Generate Well-Defined Semitelechelic Michael Acceptor Polymers

(2009) Gregory N. Grover et al.   MACROMOLECULES

In Situ ATRP-Mediated Hierarchical Formation of Giant Amphiphile Bionanoreactors

(2008) Benjamin Le Droumaguet et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Click Chemistry: A Powerful Tool to Create Polymer‐Based Macromolecular Chimeras

(2008) Benjamin Le Droumaguet et al.   MACROMOLECULAR RAPID COMMUNICATIONS

A Method for Obtaining Defined End Groups of Polymethacrylates Prepared by the RAFT Process during Aminolysis

(2008) Peter J. Roth et al.   MACROMOLECULES

Review of factors that influence the abundance of ions produced in a tandem mass spectrometer and statistical methods for discovering these factors

(2008) Sheila J. Barton et al.   MASS SPECTROMETRY REVIEWS

De Novo High-Resolution Protein Structure Determination from Sparse Spin-Labeling EPR Data

(2008) Nathan Alexander et al.   STRUCTURE