Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

The Architecture of SARS-CoV-2 Transcriptome

(2020) Dongwan Kim et al.   CELL

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

(2020) David E. Gordon et al.   NATURE

Mechanisms and Regulation of RNA Condensation in RNP Granule Formation

(2020) Devin Tauber et al.   TRENDS IN BIOCHEMICAL SCIENCES

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly

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Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites

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The Global Phosphorylation Landscape of SARS-CoV-2 Infection

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A proposed role for the SARS‐CoV‐2 nucleocapsid protein in the formation and regulation of biomolecular condensates

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Growth factor receptor signaling inhibition prevents SARS-CoV-2 replication

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Architecture and self‐assembly of the SARS‐CoV ‐2 nucleocapsid protein

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A molecular pore spans the double membrane of the coronavirus replication organelle

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Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein

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A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis

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Molecular Architecture of the SARS-CoV-2 Virus

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Liquid–liquid phase separation by SARS-CoV-2 nucleocapsid protein and RNA

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SR/RS Motifs as Critical Determinants of Coronavirus Life Cycle

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SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

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SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

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Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling

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Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Human Coronavirus: Host-Pathogen Interaction

(2019) To Sing Fung et al.   Annual Review of Microbiology

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles

(2019) Yuqin Zhou et al.   JOURNAL OF VIROLOGY

The Role of Post-Translational Modifications in the Phase Transitions of Intrinsically Disordered Proteins

(2019) Izzy Owen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle

(2019) Yingying Cong et al.   JOURNAL OF VIROLOGY

Coronavirus genomic RNA packaging

(2019) Paul S. Masters   VIROLOGY

An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence

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The Role of Phase Separation in Heterochromatin Formation, Function, and Regulation

(2018) Adam G. Larson et al.   BIOCHEMISTRY

Post-translational modifications of coronavirus proteins: roles and function

(2018) To Sing Fung et al.   Future Virology

Phase Transitions Drive the Formation of Vesicular Stomatitis Virus Replication Compartments

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New tools for automated high-resolution cryo-EM structure determination in RELION-3

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Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin

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Biomolecular condensates: organizers of cellular biochemistry

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Liquid phase condensation in cell physiology and disease

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Negri bodies are viral factories with properties of liquid organelles

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Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers

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Electron microscopy studies of the coronavirus ribonucleoprotein complex

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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

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Principles and Properties of Stress Granules

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CTFFIND4: Fast and accurate defocus estimation from electron micrographs

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Glycogen synthase kinase-3 (GSK3): Regulation, actions, and diseases

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SHAPE analysis of the RNA secondary structure of the Mouse Hepatitis Virus 5’ untranslated region and N-terminal nsp1 coding sequences

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The structure and functions of coronavirus genomic 3′ and 5′ ends

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Continuous and Discontinuous RNA Synthesis in Coronaviruses

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The Nucleocapsid Protein of Human Coronavirus NL63

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The SARS coronavirus nucleocapsid protein – Forms and functions

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PLAAC: a web and command-line application to identify proteins with prion-like amino acid composition

(2014) A. K. Lancaster et al.   BIOINFORMATICS

Nucleocapsid Phosphorylation and RNA Helicase DDX1 Recruitment Enables Coronavirus Transition from Discontinuous to Continuous Transcription

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Does form meet function in the coronavirus replicative organelle?

(2014) Benjamin W. Neuman et al.   TRENDS IN MICROBIOLOGY

Cytoplasmic RNA Granules and Viral Infection

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Characterization of a Critical Interaction between the Coronavirus Nucleocapsid Protein and Nonstructural Protein 3 of the Viral Replicase-Transcriptase Complex

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Solution Structure of Mouse Hepatitis Virus (MHV) nsp3a and Determinants of the Interaction with MHV Nucleocapsid (N) Protein

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Transient Oligomerization of the SARS-CoV N Protein – Implication for Virus Ribonucleoprotein Packaging

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Functional Transcriptional Regulatory Sequence (TRS) RNA Binding and Helix Destabilizing Determinants of Murine Hepatitis Virus (MHV) Nucleocapsid (N) Protein

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Single-molecule pull-down for studying protein interactions

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Biogenesis and Dynamics of the Coronavirus Replicative Structures

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Organelle-Like Membrane Compartmentalization of Positive-Strand RNA Virus Replication Factories

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Qualitative and quantitative ultrastructural analysis of the membrane rearrangements induced by coronavirus

(2010) Mustafa Ulasli et al.   CELLULAR MICROBIOLOGY

The Coronavirus Nucleocapsid Protein Is Dynamically Associated with the Replication-Transcription Complexes

(2010) M. H. Verheije et al.   JOURNAL OF VIROLOGY

An Interaction between the Nucleocapsid Protein and a Component of the Replicase-Transcriptase Complex Is Crucial for the Infectivity of Coronavirus Genomic RNA

(2010) K. R. Hurst et al.   JOURNAL OF VIROLOGY

Group-specific structural features of the 5′-proximal sequences of coronavirus genomic RNAs

(2010) Shih-Cheng Chen et al.   VIROLOGY

Coronavirus N Protein N-Terminal Domain (NTD) Specifically Binds the Transcriptional Regulatory Sequence (TRS) and Melts TRS-cTRS RNA Duplexes

(2009) Nicholas E. Grossoehme et al.   JOURNAL OF MOLECULAR BIOLOGY

Coronavirus Nucleocapsid Protein Facilitates Template Switching and Is Required for Efficient Transcription

(2009) S. Zuniga et al.   JOURNAL OF VIROLOGY

Identification of In Vivo-Interacting Domains of the Murine Coronavirus Nucleocapsid Protein

(2009) K. R. Hurst et al.   JOURNAL OF VIROLOGY

Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization

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Glycogen Synthase Kinase-3 Regulates the Phosphorylation of Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein and Viral Replication

(2008) Chia-Hsin Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Multiple Nucleic Acid Binding Sites and Intrinsic Disorder of Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein: Implications for Ribonucleocapsid Protein Packaging

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SARS-Coronavirus Replication Is Supported by a Reticulovesicular Network of Modified Endoplasmic Reticulum

(2008) Kèvin Knoops et al.   PLOS BIOLOGY

Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 Activation

(2008) Monique H. Verheije et al.   PLoS Pathogens

Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method

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