Article
Chemistry, Physical
Vince St Dollente Mesias, Hongni Zhu, Xiao Tang, Xin Dai, Wei Liu, Yusong Guo, Jinqing Huang
Summary: This study demonstrates the potential interactions between SARS-CoV-2 protein segments and neuronal proteins, suggesting a potential risk of post-infection neurodegeneration. The receptor-binding domain of the spike protein can inhibit the fibrillization of neuronal proteins, while a segment of the envelope protein can accelerate the formation of oligomers. These findings provide important molecular insights into the neurological effects of SARS-CoV-2 infection and its association with Parkinson's disease.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Multidisciplinary Sciences
Pengfei Li, Biyun Xue, Nicholas J. Schnicker, Lok- Yin Roy Wong, David K. Meyerholz, Stanley Perlman
Summary: The unique region (SUD) in the nonstructural protein Nsp3 of SARS-CoV-2 plays an important role in enhancing viral translation. The study identified a attenuated virus with a mutation in the linker region of Nsp3 and a compensatory mutation in the nucleocapsid protein (N) that restored virulence, revealing the impact of Nsp3-N interactions on virus replication and pathogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Dongbum Kim, Jinsoo Kim, Sangkyu Park, Minyoung Kim, Kyeongbin Baek, Mijeong Kang, Jun-Kyu Choi, Sony Maharjan, Madhav Akauliya, Younghee Lee, Hyung-Joo Kwon
Summary: The study team generated a monoclonal antibody specifically detecting the N protein of SARS-CoV-2, as well as developed an ELISA-based bait and prey system for diagnosing SARS-CoV-2 infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Pan Pan, Miaomiao Shen, Zhenyang Yu, Weiwei Ge, Keli Chen, Mingfu Tian, Feng Xiao, Zhenwei Wang, Jun Wang, Yaling Jia, Wenbiao Wang, Pin Wan, Jing Zhang, Weijie Chen, Zhiwei Lei, Xin Chen, Zhen Luo, Qiwei Zhang, Meng Xu, Geng Li, Yongkui Li, Jianguo Wu
Summary: This study reveals a unique mechanism by which SARS-CoV-2 N protein promotes NLRP3 inflammasome activation, leading to excessive inflammatory responses. The interaction between N protein and NLRP3 enhances the assembly of the NLRP3 inflammasome by facilitating binding with ASC.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Noe Juvenal Mendoza-Ramirez, Julio Garcia-Cordero, Sandra Paola Martinez-Frias, Daniela Roa-Velazquez, Rosendo Luria-Perez, Jose Bustos-Arriaga, Jesus Hernandez-Lopez, Carlos Cabello-Gutierrez, Joaquin Alejandro Zuniga-Ramos, Edgar Morales-Rios, Sonia Mayra Perez-Tapia, Martha Espinosa-Cantellano, Leticia Cedillo-Barron
Summary: This study analyzes the combination of two SARS-CoV-2 viral antigens and finds that immunization with a combination of S1 or RBD and the N protein can elicit a stronger immune response. The sera from immunized mice can recognize different variants of SARS-CoV-2. This study identifies potential antigens for the second generation of COVID-19 vaccines.
Article
Veterinary Sciences
Ruoying Li, Guanming Shao, Zi Xie, Zezhong Hu, Keyu Feng, Jiahui He, Hailong Wang, Jun Fu, Xinheng Zhang, Qingmei Xie
Summary: This study successfully constructed recombinant viruses expressing SARS-CoV-2-S and SARS-CoV-2-N proteins, which provides a reference for future research on live vector vaccines for domestic animals, pets, and wild animals.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Minghai Chen, Xian-En Zhang
Summary: The COVID-19 pandemic poses a serious threat to global public health and social stability, requiring urgent understanding of the nature and infection mechanism of the virus. Pseudotyped viruses lacking certain gene sequences provide a safer alternative for studying SARS-CoV-2 in biosafety level 2 laboratories, though there are limitations to their use.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wahyu Surya, Ernesto Tavares-Neto, Andrea Sanchis, Maria Queralt-Martin, Antonio Alcaraz, Jaume Torres, Vicente M. Aguilella
Summary: This study discusses the claim that the E protein in coronaviruses forms cation-selective channels, but found that the selectivity of these channels is very mild and depends on the lipid environment. The study also found no evidence that the E protein forms specific calcium channels or requires specific conditions for activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Tiancheng Xie, Jiaxue Yang, Chubin Fang, Jing Zhang, Hua Lin, Yalan Zhu, Tian Tang, Chuan Wang
Summary: The study investigated the viability of SARS-CoV-2 on different packaging materials at various temperatures (-40°C, -20°C, and 4°C) using the MHV-JHM strain of murine hepatitis virus as a model organism. It also evaluated the effectiveness of peracetic acid and sodium hypochlorite in eliminating MHV-JHM on plastic and cardboard surfaces.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Baoling Ying, Suzanne M. Scheaffer, Bradley Whitener, Chieh-Yu Liang, Oleksandr Dmytrenko, Samantha Mackin, Kai Wu, Diana Lee, Laura E. Avena, Zhenlu Chong, James Brett Case, LingZhi Ma, Thu T. M. Kim, Caralyn E. Sein, Angela Woods, Daniela Montes Berrueta, Gwo-Yu Chang, Guillaume Stewart-Jones, Isabella Renzi, Yen-Ting Lai, Agata Malinowski, Andrea Carfi, Sayda M. Elbashir, Darin K. Edwards, Larissa B. Thackray, Michael S. Diamond
Summary: The study showed that current vaccination can enhance protection against Omicron infections, but different vaccines have varying efficacy, which requires further investigation.
Article
Microbiology
Aissatou Aicha Sow, Felix Pahmeier, Yann Ayotte, Anais Anton, Clement Mazeaud, Tania Charpentier, Lena Angelo, Simon Woo, Berati Cerikan, Darryl Falzarano, Levon Abrahamyan, Alain Lamarre, Patrick Labonte, Mirko Cortese, Ralf Bartenschlager, Steven R. LaPlante, Laurent Chatel-Chaix
Summary: Dengue virus (DENV) is a common mosquito-borne virus and currently there are no available antiviral treatments. Researchers have identified two potential DENV inhibitors, N-phenylpyridine-3-carboxamide (NPP3C) and 6-acetyl-1H-indazole (6A1HI), which can inhibit viral replication and exhibit antiviral activity against DENV.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Vladimir R. Chechetkin, Vasily V. Lobzin
Summary: The genomic ssRNA of coronaviruses is packaged within a helical nucleocapsid, and specific assembly/packaging signals are formed through cooperative interaction with nucleocapsid proteins. These signals have been detected and reconstructed in the genomes of SARS-CoV and SARS-CoV-2, and they are closely related to the helical structure of the nucleocapsid. These findings have important implications for medical applications and basic research.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biology
J. Rafael Ciges-Tomas, Maria Luisa Franco, Marcal Vilar
Summary: This study presents the crystal structure of the SARS-CoV-2 nucleocapsid protein and its binding with guanine triphosphate. The research reveals a specific interaction between the protein and guanine, which may facilitate the viral assembly process. This finding provides a framework for designing viral assembly inhibitors.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Erica Normandin, Melissa Rudy, Nikolaos Barkas, Stephen F. Schaffner, Zoe Levine, Robert F. Padera, Mehrtash Babadi, Shibani S. Mukerji, Daniel J. Park, Bronwyn L. MacInnis, Katherine J. Siddle, Pardis C. Sabeti, Isaac H. Solomon
Summary: By analyzing tissue samples from fatal COVID-19 cases, we found that viral RNA is present in extrapulmonary tissues, and the viral variants identified in different tissues are mostly unique. Patients with a longer disease course had more high-frequency minor variants, including a tissuespecific high-frequency variant in the spike protein. These findings suggest the presence of adaptation and/or compartmentalized infection, providing insights into extrapulmonary COVID-19 symptoms and the potential for viral reservoirs.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Changhao Dai, Mingquan Guo, Yanling Wu, Ban-Peng Cao, Xuejun Wang, Yungen Wu, Hua Kang, Derong Kong, Zhaoqin Zhu, Tianlei Ying, Yunqi Liu, Dacheng Wei
Summary: A multiantibodies transistor assay has been developed for sensitive and highly precise antigen pool testing, addressing the longstanding issue of antigen pool testing. This innovation enables it to be a valuable tool for precise diagnoses and population-wide screening of COVID-19 or other epidemics in the future.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Chung-ke Chang, Tzong-Huah Wu, Chu-Ya Wu, Ming-hui Chiang, Elsie Khai-Woon Toh, Yin-Chih Hsu, Ku-Feng Lin, Yu-heng Liao, Tai-huang Huang, Joseph Jen-Tse Huang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2012)
Article
Environmental Sciences
Chih-Ching Chien, Ming-Hui Jiang, Meng-Ru Tsai, Chih-Ching Chien
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Chung-ke Chang, Ming-hui Chiang, Elsie Khai-Woon Toh, Chi-Fon Chang, Tai-huang Huang
Article
Biotechnology & Applied Microbiology
Cheng-Yi Chiang, Chia-Cheng Chou, Hsin-Yang Chang, Min-Feng Hsu, Po-Jung Pao, Ming-Hui Chiang, Andrew H-J Wang
ENZYME AND MICROBIAL TECHNOLOGY
(2020)