期刊
MARINE DRUGS
卷 19, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/md19020052
关键词
Thalassia testudinum; cytotoxicity; antitumor; anti-angiogenic; gene expression
资金
- (CITMA), Cuba [P211LH005-019]
- (CNPq), Brazil [PVE400768/2014-3]
- (Flemish VLIR collaboration program), Belgium [ZEIN2011PR383, ZEIN2016PR418]
In vitro and in vivo studies demonstrated the antitumor effects of TTE on colon tumor cell lines and a murine colorectal cancer model, including suppression of cell growth, migration, and angiogenesis, as well as promotion of antitumor immunogenic cell death. Transcription profiling and pathway analysis revealed that TTE triggers specific gene expression and autophagy stress pathways, leading to its antitumor effects.
Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 mu g/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NF kappa B specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.
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