Article
Developmental Biology
Rebecca K. Schiavo, Owen J. Tamplin
Summary: This study reveals the role of Vegfc in the emergence of HSPCs in the zebrafish dorsal aorta and suggests that it regulates HSPC fate by suppressing a myeloid cell fate.
Review
Cell Biology
Cristiana Barone, Roberto Orsenigo, Raffaella Meneveri, Silvia Brunelli, Emanuele Azzoni
Summary: Our understanding of the complexity of the developing hematopoietic system has greatly increased in recent decades. We now know that multiple HSC-independent progenitor populations during fetal development play vital roles that can affect adult physiology and lead to disease. Ontogeny has been shown to be a primary determinant of the function of critical cell types.
Review
Immunology
Serge A. van de Pavert
Summary: The ILC family, a recently described immune cell family, is involved in innate immune responses and tissue homeostasis. LTi cells, part of the ILC3 family, play a critical role in embryonic lymph node formation. Recent studies have provided insights into the origin of ILCs, including embryonic LTi cells being replaced by HSC derived progenitors in adults. This review focuses on the layered origin of the ILC3 family with an emphasis on the LTi cell lineage.
IMMUNOLOGICAL REVIEWS
(2023)
Review
Biology
Wade W. Sugden, Trista E. North
Summary: Specialized subsets of endothelial cells in different organs and regions of the vascular tree carry out unique functions, with a population of hemogenic endothelial cells transforming into hematopoietic stem cells in embryos. Recent advances in the zebrafish model have uncovered factors facilitating hemogenic commitment and the transition to blood stem cells, such as biomechanical influences and metabolic cues. Transcriptomic-based approaches have revealed key signals in the embryonic niche that regulate hematopoiesis.
Article
Multidisciplinary Sciences
Sara Menegatti, Bethany Potts, Roberto Paredes, Eva Garcia-Alegria, Syed Murtuza Baker, Valerie Kouskoff
Summary: During embryonic development, blood progenitors are derived from endothelial cells via an endothelial-to-hematopoietic transition. However, little is known about the molecular characteristics of the hemogenic endothelium and the molecular changes during this transition. In this study, we analyzed a population of human embryonic stem cell-derived endothelial cells and found that endothelial cells expressing RUNX1 exhibit enriched hemogenic potential and minimal molecular differences compared to their endothelial counterparts. Additionally, we identified CD82 as a marker of the endothelium-to-hematopoietic transition.
Article
Multidisciplinary Sciences
Wen Hao Neo, Yiran Meng, Alba Rodriguez-Meira, Muhammad Z. H. Fadlullah, Christopher A. G. Booth, Emanuele Azzoni, Supat Thongjuea, Marella F. T. R. de Bruijn, Sten Eirik W. Jacobsen, Adam J. Mead, Georges Lacaud
Summary: The study reveals the crucial role of Ezh2 in modulating Wnt signaling during the generation of EMPs from YS HE. Loss of EZH2 activity in HE leads to the generation of non-functional EMPs due to a lack of Wnt signaling downregulation, while the generation of primitive erythroid cells is not affected. EZH2 is essential for the generation of functional EMPs at the onset of the endothelial-to-hematopoietic transition but becomes dispensable later on.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Shizheng Zhao, Shachuan Feng, Ye Tian, Zilong Wen
Summary: Hematopoietic stem cells (HSCs) and conventional endothelial cells (cECs) in the dorsal aorta (DA) display spatial separation and arise from a common precursor. The Etv2 dosage plays a crucial role in governing their fate specification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
C. Biben, T. S. Weber, K. S. Potts, J. Choi, D. C. Miles, A. Carmagnac, T. Sargeant, C. A. de Graaf, K. A. Fennell, A. Farley, O. J. Stonehouse, M. A. Dawson, D. J. Hilton, S. H. Naik, S. Taoudi
Summary: By using single cell RNA-Sequencing and in vivo cellular barcoding, researchers have unraveled the ancestral relationships that give rise to the haematopoietic lineages of the yolk sac, the endothelium, and the mesenchyme. They found that blood and endothelial cells emerge from three distinct precursors: the haemangioblast, the mesenchymoangioblast, and a previously undescribed cell type called the haematomesoblast. This study is of great significance for a better understanding of embryonic development and the formation of the hematopoietic system.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yohko Kitagawa, Akihiro Ikenaka, Ryohichi Sugimura, Akira Niwa, Megumu K. Saito
Summary: Cell differentiation is achieved through the acquisition of a cell type-specific transcriptional program and epigenetic landscape. Research has shown that the patterning of cell type-specific enhancers occurs before cell fate decisions, but the mechanisms for initiating cell specification and properly restricting differentiation remain unclear. In this study, the activation of hematopoietic enhancers during arterialization of hemogenic endothelium, a key step in hematopoiesis, was observed. It was also found that the transcription factor ZEB2, involved in arterial endothelial cell regulation, and the hematopoietic regulator MEIS1 are independently required for activating these enhancers. Deficiency in ZEB2 or MEIS1 impairs hematopoietic cell development. These findings suggest that multiple regulators expressed during early development contribute non-redundantly to the establishment of the hematopoietic enhancer landscape, thereby restricting cell differentiation despite the unrestricted expression of these regulators in hematopoietic cells.
Review
Hematology
Vincenzo Calvanese, Hanna K. A. Mikkola
Summary: Developmental hematopoiesis is a complex process involving the generation of differentiated blood cells and the establishment of a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. Recent single-cell studies have identified rare human HSCs and traced their origin to a unique type of arterial endothelium in the aorta-gonad-mesonephros region. These studies provide new insights into HSC generation and may inform efforts to generate HSCs from pluripotent stem cells in vitro.
Article
Cell Biology
Elizabeth D. Howell, Amanda D. Yzaguirre, Peng Gao, Raphael Lis, Bing He, Melike Lakadamyali, Shahin Rafii, Kai Tan, Nancy A. Speck
Summary: The study highlights the role of RUNX1 in hematopoiesis, showing its ability to recruit TGFβ signaling effectors in the endothelial-to-hematopoietic transition. The efficiency of this transition is influenced by chromatin changes in endothelial cells at different developmental stages.
GENES & DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Leal Oburoglu, Els Mansell, Niels-Bjarne Woods
Summary: Glutamine metabolism plays an essential role in regulating the emergence and specification of hematopoietic cells during endothelial to hematopoietic transition (EHT), with different hematopoietic lineages requiring distinct derivatives of glutamine.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Cell Biology
Ann Sanoji Samarakkody, Alan B. Cantor
Summary: The study reveals the mechanism by which RUNX1 collaborates with TGF beta signaling transcription factors to drive chromatin accessibility changes that specify hemogenic endothelial cells (HECs). It also highlights interesting parallels between hematopoietic transition (EHT) and endothelial-to-mesenchymal transition (EndoMT) during cardiac development.
GENES & DEVELOPMENT
(2021)
Article
Cell & Tissue Engineering
Emmanuelle Julien, Katia Biasch, Reine El Omar, Jean-Noel Freund, Christian Gachet, Francois Lanza, Manuela Tavian
Summary: The study found that angiotensin-converting enzyme is expressed in hematopoietic progenitors in developing mouse embryos and various elements of the renin-angiotensin system are present in specific regions of human and mouse embryos. Experimentally, angiotensin II signaling is crucial for the generation of hematopoietic cells, with the peptide stimulating hematopoiesis in culture.
Article
Cell & Tissue Engineering
J. Philip Creamer, Stephanie A. Luff, Hao Yu, Christopher M. Sturgeon
Summary: The expression of CDX4+CD1d+ marks an early progenitor of hematopoietic endothelium with intra-embryonic-like potential in early mesoderm, providing important insights for the study of human hematopoietic development and potential improvements in hematopoietic differentiation conditions for regenerative medicine applications.
STEM CELL RESEARCH
(2022)
Review
Hematology
Charles Durand, Pierre Charbord, Thierry Jaffredo
CURRENT OPINION IN HEMATOLOGY
(2018)
News Item
Medicine, Research & Experimental
Gregoire Stik, Laurence Petit, Pierre Charbord, Thierry Jaffredo, Charles Durand
M S-MEDECINE SCIENCES
(2018)
Article
Multidisciplinary Sciences
Chloe S. Baron, Lennart Kester, Anna Klaus, Jean-Charles Boisset, Roshana Thambyrajah, Laurent Yvernogeau, Valerie Kouskoff, Georges Lacaud, Alexander van Oudenaarden, Catherine Robin
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Giuliano Ferrero, Christopher B. Mahony, Eleonore Dupuis, Laurent Yvernogeau, Elodie Di Ruggiero, Magali Miserocchi, Marianne Caron, Catherine Robin, David Traver, Julien Y. Bertrand, Valerie Wittamer
Letter
Hematology
Loic Maillard, Sandra Sanfilippo, Carine Domenech, Nassima Kasmi, Laurence Petit, Sebastien Jacques, Anne-Lise Delezoide, Fabien Guimiot, Soria Eladak, Delphine Moison, Nour Nicolas, Virginie Rouiller-Fabre, Stephanie Pozzi-Godin, Benoit Mennesson, Marie-Laure Brival, Franck Letourneur, Thierry Jaffredo, Christine Chomienne, Michele Souyri
Article
Cell Biology
Laurent Yvernogeau, Rodolphe Gautier, Laurence Petit, Hanane Khoury, Frederic Relaix, Vanessa Ribes, Helen Sang, Pierre Charbord, Michele Souyri, Catherine Robin, Thierry Jaffredo
NATURE CELL BIOLOGY
(2019)
Article
Biology
Katrina M. Morris, Matthew M. Hindle, Simon Boitard, David W. Burt, Angela F. Danner, Lel Eory, Heather L. Forrest, David Gourichon, Jerome Gros, LaDeana W. Hillier, Thierry Jaffredo, Hanane Khoury, Rusty Lansford, Christine Leterrier, Andrew Loudon, Andrew S. Mason, Simone L. Meddle, Francis Minvielle, Patrick Minx, Frederique Pitel, J. Patrick Seiler, Tsuyoshi Shimmura, Chad Tomlinson, Alain Vignal, Robert G. Webster, Takashi Yoshimura, Wesley C. Warren, Jacqueline Smith
Article
Hematology
Laurent Yvernogeau, Anna Klaus, Joris Maas, Ismael Morin-Poulard, Bart Weijts, Stefan Schulte-Merker, Eugene Berezikov, Jan Philipp Junker, Catherine Robin
Article
Multidisciplinary Sciences
Christophe Desterke, Laurence Petit, Nadir Sella, Nathalie Chevallier, Vincent Cabeli, Laura Coquelin, Charles Durand, Robert A. J. Oostendorp, Herve Isambert, Thierry Jaffredo, Pierre Charbord
Review
Developmental Biology
Laurent Yvernogeau, Giovanna Dainese, Thierry Jaffredo
Summary: Recent studies have shown that the aorta microenvironment plays a crucial role in the generation of the first haematopoietic stem cells (HSCs) from specialized haemogenic endothelial cells (HECs). However, there is still a need for a deeper understanding of the cellular and molecular events involved in aorta formation and polarization, which are essential for HEC specification and HSC competency. This review compares the early mechanisms of aorta formation in different vertebrate species and discusses how the molecular signals from the aorta environment result in its polarization, creating a unique entity for HSC generation.
Article
Mathematical & Computational Biology
Thanh Nam Nguyen, Jean Clairambault, Thierry Jaffredo, Benoit Perthame, Delphine Salort
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2019)