Article
Biochemistry & Molecular Biology
Eva Cifkova, Radana Brumarova, Magdalena Ovcacikova, Dana Dobesova, Katerina Micova, Ales Kvasnicka, Zuzana Vankova, Jiri Siller, Lukas Sakra, David Friedecky, Michal Holcapek
Summary: The study conducted lipidomic and metabolomic analysis on tumor and surrounding normal tissues of 23 patients with non-small cell lung cancer, identifying and quantifying 500 molecular species. Significant changes were observed in several biochemical pathways related to lung cancer, particularly involving glycerophospholipids, sphingolipids, and cholesteryl esters in lipid metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Toxicology
Jermaine Ford, Cal Riutta, Patricia A. Kosian, Katherine O'Shaughessy, Mary Gilbert
Summary: Numerous xenobiotics can affect thyroid hormone signaling. Measuring thyroid hormone in the brain is more directly linked to neurodevelopmental toxicity induced by thyroid system-disrupting chemicals. However, extracting and measuring thyroid hormone in the brain tissue is challenging. This study reports optimized analytical procedures for thyroid hormone extraction in rat brain tissue and reveals age-dependent increases in brain thyroid hormone levels.
TOXICOLOGICAL SCIENCES
(2023)
Article
Engineering, Environmental
Nguyen Hoang Anh, Young Jin Min, Truong Thi My Nhung, Nguyen Phuoc Long, Seunghyeon Han, Sun Jo Kim, Cheol Woon Jung, Young Cheol Yoon, Yun Pyo Kang, Sang Ki Park, Sung Won Kwon
Summary: This study conducted a gene pathway meta-analysis to identify common and divergent key events associated with silver nanoparticles (AgNPs) and explore their patterns across species. It found that oxidative stress, apoptosis, and metabolite and lipid metabolism pathways may be potentially conserved key events across species. Oxidative stress responses were confirmed as early key events in both Caenorhabditis elegans and HepG2 cells, and AgNPs preferentially impaired mitochondria. These findings provide insights into the development of adverse outcome pathways of AgNPs and regulatory toxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Agriculture, Multidisciplinary
Xianlin Han, Hongping Ye
Summary: Although triglycerides play important roles in cellular growth, metabolism, and function, accurately identifying their molecular species and quantities remains a challenge.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Xina Yu, Qingcheng Wang, Weibo Lu, Min Zhang, Kang Chen, Jing Xue, Qiaoling Zhao, Pingya Wang, Pei Luo, Qing Shen
Summary: An PreIS-HILIC-MS method was established to specifically and selectively screen PLEPA/DHA in different fish oil samples, including saury, grass carp, hairtail, and yellow croaker. The method demonstrated high sensitivity, good linearity, and suitability for quantitative analysis of PLEPA/DHA in fish oil samples, with the ability to identify and quantify 24 different compounds. The performance of this method was compared with traditional full-scan mode and showed unique superiority in targeted screening of PLEPA/DHA in fish oils.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Marine & Freshwater Biology
Mackenzie L. Morshead, Kathleen M. Jensen, Gerald T. Ankley, Sara Vliet, Carlie A. LaLone, Alejandro Vidales Aller, Karen H. Watanabe, Daniel L. Villeneuve
Summary: The aim of this study was to explore the potential mechanisms through which exposure to estrogen receptor agonists might lead to reproductive impairment in female fish. The results revealed that exposure to EE2 or BPA, as well as their mixtures with TRB, significantly decreased plasma concentrations of vitellogenin in female fish. In contrast, exposure to TRB alone caused a significant reduction in plasma vitellogenin. Based on these findings, it is hypothesized that the impaired oocyte maturation and ovulation, possibly due to negative feedback or direct inhibitory effects of membrane estrogen receptor activation, could be responsible for the adverse reproductive impacts in female fish exposed to estrogen receptor agonists.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cristina Manis, Antonio Murgia, Alessia Manca, Antonella Pantaleo, Giacomo Cao, Pierluigi Caboni
Summary: Our study using a hyphenated ion mobility mass spectrometric platform for lipid profiling of human erythrocytes expands the understanding of cellular processes and stress due to microgravity. We identified complex lipids associated with microgravity conditions, such as oxidized phosphocholines, phosphocholines bearing arachidonic in their moiety, as well as sphingomyelins and hexosyl ceramides. These findings provide insights into molecular alterations and erythrocyte lipidomics signatures associated with microgravity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Lauren M. Bishop, Oliver Fiehn
Summary: Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Fisheries
Pauline Jehannet, Arjan P. Palstra, Ignacio Gimenez Nebot, Henk Schipper, William Swinkels, Leon T. N. Heinsbroek, Hans Komen
Summary: The effects of eel-specific recombinant gonadotropins (rGTHs) on oocyte development in European eels were tested in this study. The results showed that treatment with recombinant luteinizing hormone (rLH) induced oocyte development in vitro, and treatment with recombinant follicle-stimulating hormone (rFSH) followed by rLH stimulated sexual maturation in vivo. Larvae were produced for the first time by rGTH treatment alone, but further optimization is needed for dosage and timing.
Article
Fisheries
Shany Cohen, Omer Ilouz, Rivka Manor, Amir Sagi, Isam Khalaila
Summary: In this study, silencing of the VIH gene alone or in combination with the MIH gene successfully induced ovarian maturation and development in females of Macrobrachium rosenbergii, with more pronounced effects in mature individuals. Additionally, the dsRNA used to silence VIH also affected the expression of MIH, albeit with a delay compared to the dsRNA targeting both VIH and MIH simultaneously.
Article
Biochemistry & Molecular Biology
Sin Man Lam, Jie Li, Huan Sun, Weining Mao, Zongmin Lu, Qingshuo Zhao, Chao Han, Xia Gong, Binhua Jiang, Gek Huey Chua, Zhenwen Zhao, Fanwei Meng, Guanghou Shui
Summary: The neural compartmentalization of lipid distribution and metabolism is associated with troglomorphic traits in Sinocyclocheilus cavefish. This includes reductions in DHA-phospholipids and accumulation of arachidonic acid-phospholipids in the brain, demyelination in serotonergic neurons, and systemic reductions in esterified DHAs in the liver. These adaptations serve to reduce energy expenditure and are crucial for the survival of cavefish in cave environments.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Agriculture, Dairy & Animal Science
Andrea Grana-Baumgartner, Venkata S. R. Dukkipati, Patrick J. Biggs, Paul R. Kenyon, Hugh T. Blair, Nicolas Lopez-Villalobos, Alastair B. Ross
Summary: This study evaluated the impact of short-term cold exposure on new-born lamb brown adipose tissue and plasma lipid composition. The results showed that cold exposure induced significant changes in the lipidome composition, potentially enhancing lipid metabolism and survival during cold adaptation. The study suggests that identifying potential biomarkers for brown adipose tissue activity could improve survival in new-born lambs.
Article
Chemistry, Applied
Lijun Ge, Huicheng Yang, Weibo Lu, Yiwei Cui, Shikai Jian, Gongshuai Song, Jing Xue, Xin He, Qingcheng Wang, Qing Shen
Summary: In this study, the content and composition of POA and POA lipids in six seafood by-products were analyzed using UHPLC-QE/MS. The results showed significant variations in the relative content of POA among different fish species, and the presence and ratio of POA-PL and POA-DG/TG could be used to identify different fish species.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Diana Lopes, Tania Melo, Felisa Rey, Elisabete Costa, Ana S. P. Moreira, Maria H. Abreu, Pedro Domingues, Ana Lillebo, Ricardo Calado, M. Rosario Domingues
Summary: The valorization of seaweeds has gained attention for their unique chemical characteristics, particularly in the differentiation between Chlorophyta, Rhodophyta, and Ochrophyta. This study analyzed polar lipidomes of six seaweeds to identify species-specific lipid signatures, with a small group of shared lipid species among all seaweed species. Understanding the specific lipidome of seaweeds can lead to new strategies for chemical tuning during farming to improve the yield of bioactive compounds for sustainable growth in a blue bioeconomy.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Cell Biology
Christopher L. Souders, Jasenka Zubcevic, Christopher J. Martyniuk
Summary: The colonic epithelium plays a crucial role in the production and transport of vasoactive metabolites and neurotransmitters that modulate the immune system, affect cellular metabolism, and regulate blood pressure. Inflammation in the colon and microbial metabolites have been highlighted in the pathology of hypertension, particularly the interaction between TNF alpha and SCFA metabolites. This review identifies novel pathways in the colonic epithelium related to hypertension and emphasizes the complexity of host-microbiota interactions in regulating blood pressure and metabolic function.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Environmental Sciences
Bernard Robaire, Geraldine Delbes, Jessica A. Head, Vicki L. Marlatt, Christopher J. Martyniuk, Stephane Reynaud, Vance L. Trudeau, Jan A. Mennigen
Summary: Studies have mainly focused on the effects of EDCs on adult males and females, but exposure of mature or developing gametes can lead to multigenerational and transgenerational effects. By synthesizing knowledge across vertebrate species, potential mechanisms of EDC effects are discussed, and recommendations for advancing the field are proposed.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
V. L. Marlatt, S. Bayen, D. Castaneda-Cortes, G. Delbes, P. Grigorova, V. S. Langlois, C. J. Martyniuk, C. D. Metcalfe, L. Parent, A. Rwigemera, P. Thomson, G. Van der Kraak
Summary: This review summarizes the current knowledge of the impacts of EDCs on reproductive success in wildlife and humans, with a focus on retrospective assessment and laboratory-based experiments. The evidence suggests that anthropogenic chemicals negatively impact reproduction by altering endocrine system function, particularly in aquatic species. Similar effects are observed in mammalian wildlife and humans in epidemiological studies. Further research is needed on the implications of chemical exposures during development and the potential for long-term reproductive effects. Field-based observations and long-term population level studies are key to addressing knowledge gaps.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Julie Robitaille, Nancy D. Denslow, Beate Escher, Hajime G. Kurita-Oyamada, Vicki Marlatt, Christopher J. Martyniuk, Laia Navarro-Martin, Ryan Prosser, Thomas Sanderson, Viviane Yargeau, Valerie S. Langlois
Summary: This paper discusses the hazards of endocrine disrupting chemicals (EDCs) in water resources, the potential bioassays for screening EDCs, advantages and limitations of sample preparation methods, and provides several key recommendations.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Jiye Zhang, Huihui Chen, Tianheng Tong, Ruimin Liu, Saihong Yan, Xuefang Liang, Christopher J. Martyniuk, Jinmiao Zha
Summary: Asian clams (Corbicula fluminea) exposed to different concentrations of Benzotriazole ultraviolet stabilizers (BUVSs) showed varying physiological and molecular toxic effects, including changes in filtration rate, histopathological changes, and induction of apoptosis. This study provides important insights into the toxicity and hazards of BUVSs in benthic organisms.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Emma Ivantsova, Isaac Konig, Christopher L. Souders II, David McNabney, Denina D. B. Simmons, Christopher J. Martyniuk
Summary: Blood pressure medication atenolol, a beta-blocker detected in wastewater systems, has negative effects on early stages of zebrafish development. It affects survival, heart rate, mitochondrial function, lipid and amino acid profiles, and locomotor activity. Atenolol decreases heart rate and alters lipid content in zebrafish at environmentally relevant levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Nazish Iftikhar, Isaac Konig, Cole English, Emma Ivantsova, Christopher L. L. Souders II, Imran Hashmi, Christopher J. J. Martyniuk
Summary: The broad-range bacteriostatic antibiotic sulfamethoxazole (SMX) has been found to have sub-lethal toxicity effects on developing zebrafish embryos and larvae, including decreased survivability, delayed hatch, and induced malformations. It also triggers an inflammatory response and alters the expression of genes related to apoptosis. These findings contribute to the understanding of antibiotic toxicity in aquatic organisms and provide a foundation for environmental risk assessment of SMX and other antibiotics.
Article
Multidisciplinary Sciences
Cole D. English, Kira J. Kazi, Isaac Konig, Emma Ivantsova, Christopher L. Souders Ii, Christopher J. Martyniuk
Summary: Zebrafish larvae were exposed to different concentrations of Ifosfamide, and RNA-seq analysis revealed 21 microRNAs that were associated with Ifosfamide exposure. This study provides valuable insights for the advancement of regulatory toxicology and biomarker discovery in aquatic environments.
Article
Environmental Sciences
Mohamed Hamed, Christopher J. Martyniuk, Rashad E. M. Said, Hamdy A. M. Soliman, Ahmed E. A. Badrey, Elhagag A. Hassan, Hani N. Abdelhamid, Alaa G. M. Osman, Alaa El -Din H. Sayed
Summary: This study reports the presence of pyrogallol in wastewater in Egypt for the first time. Acute and sub-acute toxicity experiments were conducted to evaluate the toxic effects of pyrogallol on catfish. The results showed that pyrogallol exposure caused morphological changes, hematological and biochemical alterations, indicating potential risks to aquatic species.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Wei Chi, Juanjuan Fu, Chris J. Martyniuk, Jiangyong Wang, Libin Zhou
Summary: This study investigates the collaboration of HIF-1 alpha A and HIF-1 alpha B in regulating mitophagy and apoptosis under hypoxic stress in cyprinid fish. The results show that HIF-1 alpha A induces apoptosis by promoting ROS generation and mitochondrial depolarization, while HIF-1 alpha B is primarily responsible for mitophagy induction, promoting ATP production to mitigate apoptosis.
JOURNAL OF MOLECULAR EVOLUTION
(2023)
Article
Endocrinology & Metabolism
Chunyu Lu, Di Peng, W. C. K. Udeesha Erandani, Kimberly Mitchell, Christopher J. Martyniuk, Vance L. Trudeau
Summary: The detection and quantification of hormones are crucial for assessing experimental models and diagnosing diseases. Traditional methods involve separate experiments, while the new approach allows simultaneous measurement of different categories of hormones. This study presents a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Sarah J. Patuel, Cole English, Victoria Lopez-Scarim, Isaac Konig, Christopher L. Souders II, Emma Ivantsova, Christopher J. Martyniuk
Summary: The study investigates the effects of Broflanilide, a novel pesticide, on zebrafish larvae. It reveals that exposure to different concentrations of Broflanilide can impact gene networks associated with various neurological disorders, including axonal injury, depression, neuroinflammation, brain infarction, and excitotoxicity. The study also identifies genes related to MPTP-induced neurotoxicity, which has relevance for Parkinson's disease.
Article
Environmental Sciences
David W. Bertolatus, Larry B. Barber, Christopher J. Martyniuk, Huajun Zhen, Timothy W. Collette, Drew R. Ekman, Aaron Jastrow, Jennifer L. Rapp, Alan M. Vajda
Summary: In order to evaluate the relationships between anthropogenic impacts, contaminant occurrence, and fish health, fish exposures were conducted across different land use sites in the Shenandoah River watershed. The study found adverse reproductive outcomes and increased mortality in fish exposed to wastewater treatment plant effluent and agricultural impacts. Molecular biomarkers and hepatic metabolomic and transcriptomic profiles indicated variations in land use and contaminant profiles. This study demonstrated the linkages between human impacts, contaminant occurrence, and exposure effects, highlighting the increased risk of adverse outcomes in fish exposed to complex mixtures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Isaac Konig, Nazish Iftikhar, Evelyn Henry, Cole English, Emma Ivantsova, Christopher L. Souders II, Silvana Marcussi, Christopher J. Martyniuk
Summary: This study compared the toxicity of carvacrol, acetylcarvacrol, and fipronil to early staged zebrafish. The results showed that carvacrol and its derivative have lower acute toxicity compared to fipronil.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Environmental Sciences
Sarah J. Patuel, Cole English, Victoria Lopez-Scarim, Isaac Konig, Christopher L. Souders II, Emma Ivantsova, Christopher J. Martyniuk
Summary: This study evaluates the sub-lethal toxicity potential of broflanilide in early staged zebrafish and finds that the insecticide has minimal impact on survival and biochemical parameters of the fish but can induce neurotoxicity and motor dysfunction.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)