Article
Cell Biology
Shoichiro Ono, Mario Lewis, Kanako Ono
Summary: This study demonstrates the mutual dependence of tropomodulin and tropomyosin in sarcomere assembly and regulation of actin filaments in the nematode Caenorhabditis elegans.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Chemistry, Applied
Chia-Lin Tsai, Kristy Perng, Yu-Chen Hou, Cheng-Jou Shen, I-Ning Chen, Yi-Tien Chen
Summary: This study investigated the effects of species, muscle location, food processing, and refrigerated storage on fish tropomyosin and compared it with the main fish allergen, parvalbumin. The results showed that tropomyosin is the most abundant and thermally stable protein in fish muscle. It also provides valuable information for the detection of major fish allergens.
Article
Biochemistry & Molecular Biology
Elumalai Pavadai, Michael J. Rynkiewicz, Zeyu Yang, Ian R. Gould, Steven B. Marston, William Lehman
Summary: This study reveals the phosphorylation regulation mechanism of cardiac-specific tropomyosin by utilizing protein modeling and molecular dynamics simulation. The results suggest that phosphorylated residues may interact closely with tropomyosin and the N-lobe of TnC, thereby affecting cardiac muscle contraction and relaxation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Chanchanok Chaichim, Tamara Tomanic, Holly Stefen, Esmeralda Paric, Lucy Gamaroff, Alexandra K. Suchowerska, Peter W. Gunning, Yazi D. Ke, Thomas Fath, John Power
Summary: The study found that, despite being enriched in dendritic spines, overexpression of Tpm3.1 does not significantly alter the morphology or function of dendritic spines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Yi-Tien Chen, Yun-Hwa Peggy Hsieh
Summary: The 36 kDa antigenic protein of a fish-specific monoclonal antibody, known as 8F5, was characterized as tropomyosin and its fish-specific epitope was identified. This research has significant implications for the immunodetection of fish in cooked food.
Article
Biochemistry & Molecular Biology
Natalia A. Koubassova, Andrey K. Tsaturyan
Summary: Contraction of cardiac muscle is regulated by Ca2+ ions via regulatory proteins, troponin (Tn), and tropomyosin (Tpm) associated with the thin (actin) filaments in myocardial sarcomeres. Recent cryo-electron microscopy (cryo-EM) models of the complex allow one to study the dynamic and mechanical properties of the complex using molecular dynamics (MD). However, further refinement of the models is needed to improve the protein-protein interaction in some regions of the complex, as suggested by problems revealed from the MD simulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Olof Regnell, Sylvie V. M. Tesson, Nikolay Oskolkov, Michelle Nerentorp
Summary: A study found a positive correlation between selenium and mercury in freshwater fish. Selenium mitigates the toxicity of mercury but can divert from biosynthetic pathways. The study also observed a surplus of selenium in fish and found that it decreased with fish weight. This suggests that selenium accumulation is subject to homeostatic control and is related to the burden of mercury and selenium requirement.
WATER AIR AND SOIL POLLUTION
(2022)
Article
Cell Biology
Brian K. Haarer, Morgan L. Pimm, Ebbing P. de Jong, David C. Amberg, Jessica L. Henty-Ridilla
Summary: Biochemical studies of human actin and its binding partners have mainly used a-actin from skeletal muscle, which is abundant and easily purified. However, there is concern that these proteins may behave differently from actin in non-muscle cells. To address this, researchers developed Saccharomyces cerevisiae strains that express beta- or.-actin as their sole source of actin, allowing for the study of cytoplasmic actins. These purified beta- or.-actin isoforms polymerize and interact with various binding partners, including profilin, mDia1, fascin, and thymosin-beta 4 (T beta 4). The higher affinity of T beta 4 and profilin for beta- or.-actin compared to a-actin highlights the importance of testing actin ligands with specific isoforms.
JOURNAL OF CELL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Dmitri Tolkatchev, Carol C. Gregorio, Alla S. Kostyukova
Summary: Leiomodin is an important emerging regulator of thin filaments, and structural and cellular studies have helped elucidate its function mechanisms, with many domains still remaining unstudied. Future investigations may provide evidence that leiomodin is a multifunctional protein.
Article
Medicine, Research & Experimental
Jennifer McAdow, Shuo Yang, Tiffany Ou, Gary Huang, Matthew B. Dobbs, Christina A. Gurnett, Michael J. Greenberg, Aaron N. Johnson
Summary: Nemaline myopathy, as well as other musculoskeletal disorders, is caused by pathogenic variants in the Tropomyosin 2 gene, leading to muscle development and function issues. Through experiments in Drosophila, mice, and zebrafish models, the pathomechanisms of several TPM2 variants were revealed, and it was found that these variants can cause musculoskeletal defects. These assays suggest that our myogenic experiments can predict the clinical severity of TPM2 variants.
Article
Chemistry, Physical
Anthony P. Baldo, Jil C. Tardiff, Steven D. Schwartz
Summary: This study explores how tropomyosin movement on actin filaments regulates the cross-bridge cycle of cardiac muscle, with experimental information lacking on how myosin binds to the thin filament and influences tropomyosin's transition. Using metadynamics, the researchers found differences in required work due to cardiac troponin T's influence, leading to preferential myosin binding sites and determining the mechanism of further myosin head recruitment.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Multidisciplinary Sciences
Theresia Reindl, Sven Giese, Johannes N. Greve, Patrick Y. Reinke, Igor Chizhov, Sharissa L. Latham, Daniel P. Mulvihill, Manuel H. Taft, Dietmar J. Manstein
Summary: This article describes the effects of N-terminal acetylation of various tropomyosin isoforms on the actin affinity and thermal stability of actin-tropomyosin cofilaments. Additionally, it explores how the exchange of cytoskeletal tropomyosin isoforms and their acetylation impacts the kinetic and chemomechanical properties of cytoskeletal actin-tropomyosin-myosin complexes. The results highlight the extent of differences in kinetic and functional properties among different actin-tropomyosin-myosin complexes.
Review
Biochemistry & Molecular Biology
Vera B. Dugina, Galina S. Shagieva, Anton S. Shakhov, Irina B. Alieva
Summary: Endothelial cells (EC) line the inner surface of vessels and regulate vascular permeability, with their actin cytoskeleton crucial for maintaining endothelial barrier function. Non-muscle cells express two actin isoforms, beta and gamma, which have different roles in cell survival, cell division, and neoplastic transformation depending on the cell type. The spatial organization of cytoplasmic actins in different cell types, particularly in endothelial cells, is closely linked to their functional activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tsering Stobdan, Pritesh P. Jain, Mingmei Xiong, Vineet Bafna, Jason X-J Yuan, Gabriel G. Haddad
Summary: This study reveals the potential functional role of TMOD3 in high-altitude pulmonary hypertension, suggesting that lower levels of TMOD3 play an important role in the maintenance or neo-vascularization of pulmonary arteries under chronic hypoxia.
HUMAN MOLECULAR GENETICS
(2022)
Review
Physiology
J. M. Chalovich, L. Zhu, D. Johnson
Summary: Striated muscle contraction is inhibited by several actin associated proteins, and the binding of Ca2+ can relieve this inhibition. Tropomyosin can be positioned in three different states on actin, which are associated with different functional states of the contractile system. Mutants of troponin can stabilize different states and provide tools for studying individual states. The C-terminal region of troponin T plays a unique role in limiting Ca2+ activation.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Environmental Sciences
Haowen Jiang, Ying Wang, Zhiqiang Tan, Ligang Hu, Jianbo Shi, Guangliang Liu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: This study developed an online technique that combines hollow fiber ultrafiltration (HFUF) with single particle-inductively coupled plasma mass spectrometry (SP-ICP-MS) to improve the accuracy and size detection limit of metal-containing nanoparticles (MCNs) by removing metal ions. Through systematic optimization of conditions, HFUF can completely remove metal ions while retaining the particles, allowing accurate size determination.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Environmental Sciences
Dingyi Wang, Tingting Zhang, Xudong Guo, Dayi Ling, Ligang Hu, Guibin Jiang
Summary: Reducing fossil fuel usage is crucial for achieving carbon neutrality, but current energy policies may hinder economic development and people's livelihood. Three-dimensional printing (3DP) is an environmentally friendly manufacturing technology with the potential to contribute to carbon neutrality. It can reduce greenhouse gas emissions in manufacturing and construction sectors, enable the development of novel energy-related devices and technologies, and promote the production of cultured meat.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Chemistry, Analytical
Xiangwei Tian, Haowen Jiang, Meng Wang, Wenbin Cui, Yingying Guo, Lingna Zheng, Ligang Hu, Guangbo Qu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: To meet the demand for multi-element/isotope analysis at the single nanoparticle (NP) or cell level, different types of inductively coupled plasma mass spectroscopy (ICP-MS) have been used to simultaneously monitor multiple mass-to-charge ratios in single-particle/cell ICP-MS (SP/SC-ICP-MS) analysis. Systematic evaluation and comparison of the performance of these techniques are urgently required.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Qing Yang, Mengzhu Cheng, Qing Zhou, Lihong Wang, Yunlong Jiao, Yongqiang Liu, Shuya Zhang, Li Tan, Zhenhong Gu, Hong Zhu, Hongli Luo, Daozhe Lin, Nian Liu, Xiaohua Huang, Ligang Hu
Summary: The accumulation of various pollutants in leaf cells of different plants in Nanjing was significantly higher than those grown in Haikou, especially for BaP, Cd, and REEs. The activation of endocytosis in leaf cells by REEs led to the co-accumulation of BaP, Cd, and REEs, magnifying their toxic effects on plants. Moreover, the co-accumulation of pollutants in human cells was also increased by REEs.
Article
Environmental Sciences
Yaqian Xu, Yu Hu, Xin Wang, Xianping Wei, Qingqing Zhu, Ligang Hu, Chunyang Liao, Guibin Jiang
Summary: The aim of this study was to investigate the exposure of novel high-molecular-weight synthetic antioxidants in children's urine from different regions in China. The study found that the analytes were detected in children's urine, and significant differences were observed in the concentrations of certain synthetic antioxidants between the eastern and western regions of China. The study also assessed the health risk of the synthetic antioxidants and found that the estimated daily intakes were lower than the tolerable daily intake values, indicating a low health risk to children.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Shiyu Li, Lin Yang, Jialong Wu, Linlin Yao, Deming Han, Yong Liang, Yongguang Yin, Ligang Hu, Jianbo Shi, Guibin Jiang
Summary: Developing practical and cost-effective adsorbents for mercury (Hg) remediation is crucial for protecting the aquatic environment and public health. In this study, a recyclable hierarchical MoS2/Fe3O4 nanocomposite was developed for the selective removal of Hg(II) from aquatic samples. The nanocomposite exhibited high adsorption capacity, fast kinetics, broad working pH range, excellent selectivity, and great reusability. It showed promising efficiency in removing Hg(II) from natural water and industrial effluents. The study provides insights into the mechanism of Hg(II) removal and highlights the potential of MoS2/Fe3O4 for sustainable water treatments.
Article
Multidisciplinary Sciences
Haibo Wang, Ligang Hu, Hongyan Li, Yau-Tsz Lai, Xueying Wei, Xiaohan Xu, Zhenkun Cao, Huiming Cao, Qianya Wan, Yuen-Yan Chang, Aimin Xu, Qunfang Zhou, Guibin Jiang, Ming-Liang He, Hongzhe Sun
Summary: In this study, the researchers identified the Cr(III)-proteome in cells and found that ATP synthase plays a crucial role in relieving hyperglycemia stress.
NATURE COMMUNICATIONS
(2023)
Article
Environmental Sciences
Ke Yuan, Lan Lin, Xiuqin Xie, Zhiqiang Zhao, Ligang Hu, Tiangang Luan, Baowei Chen
Summary: In this study, metagenomic approaches were used to analyze water samples collected from the Pearl River and Pearl River Estuary for the presence of pathogenic bacteria. A total of 71 putative pathogenic bacterial species were identified, with Bacteroidetes being the predominant phylum. The concentration of pathogenic bacteria ranged from 1.59 x 107 to 1.46 x 108 cells/L, with a decreasing trend from the Pearl River to the Pearl River Estuary. Pathogenic particle-attached bacteria accounted for a significant proportion of the total pathogenic bacteria, and various virulence factor genes were detected, with those related to bacterial adherence being the most abundant. This study highlights the importance of suspended particulate matters in the transport of pathogenic bacteria and suggests that particle-attached bacteria may contribute to potential health risks.
Review
Chemistry, Analytical
Junpeng Zhang, Dingyi Wang, Yingying Li, Lihong Liu, Yong Liang, Bin He, Ligang Hu, Guibin Jiang
Summary: The latest progress in 3D printing technology in the field of environmental analysis is comprehensively reviewed in this paper. By optimizing printing materials, enhancing equipment structure, and integrating multidisciplinary technology, 3D printing offers opportunities to solve the challenges faced by traditional processing techniques and promote the high performance and versatility of environmental analysis devices.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Lei Li, Anthony S. Wexler, Xue Li, Ligang Hu, Guibin Jiang
Summary: Bioaerosol transmission is an important pathway for COVID-19 and other infectious respiratory diseases caused by viral infection. In situ and real-time detection of bioaerosols and their encapsulated pathogens is crucial for early warning and monitoring of epidemics and pandemics. However, the lack of a powerful analytical tool for distinguishing between bioaerosols and nonbioaerosols, as well as identifying pathogen species, is a major challenge in this field.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Hongzhe Dong, Lihong Liu, Qinfei Zhou, Yinyin Tang, Huiling Wang, Yongguang Yin, Jianbo Shi, Bin He, Yanbin Li, Ligang Hu, Guibin Jiang
Summary: This study investigated the formation, distribution, and excretion of mercury nanoparticles in diatom Chaetoceros curvisetus, and found that these nanoparticles play crucial roles in understanding mercury fate in the ocean and its burial in sediments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Yuping Xiang, Yingying Guo, Guangliang Liu, Yanwei Liu, Maoyong Song, Jianbo Shi, Ligang Hu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: In this study, a whole-cell fluorescent biosensor based on Escherichia coli was developed to investigate the bioavailability and microbial activation process of mercury sulfide nanoparticles (HgSNPs). The results showed that HgSNPs had high bioavailability and were partially dissolved inside E. coli cells. The findings highlight the importance of microbial uptake and dissolution of HgSNPs.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Ying Wang, Guangliang Liu, Yingying Fang, Peng Liu, Yanwei Liu, Yingying Guo, Jianbo Shi, Ligang Hu, Yong Cai, Yongguang Yin, Guibin Jiang
Summary: This study verifies the importance of mercurous species (Hg(I)) in natural water contaminated by liquid elemental mercury droplet (Hg(0)l) and in simulating the dark oxidation process of Hg(0)l. The formation and transformation of Hg(I) control the overall oxidation of Hg(0)l and are influenced by environmental factors. The conversion of Hg(0) to Hg(I) is the rate-limiting step in the oxidation process, and ligands like OH-, Cl-, and natural organic matter can enhance the formation of Hg(I).
Article
Engineering, Environmental
Zelin Shen, Guangliang Liu, Yingying Guo, Tao Jiang, Yanwei Liu, Jianbo Shi, Ligang Hu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: The aging kinetics of divalent mercury (Hg(II)) mediated by dissolved organic matter (DOM) play a significant role in controlling Hg(II) transformation and bioavailability in natural aquatic environments. However, the differential behaviors of new and aged Hg(II) in the same reaction system remain unknown. This study used multi-isotope tracing to investigate the impacts of binding site and sulfidation during DOM-mediated Hg(II) aging processes on Hg(II) reduction and microbial methylation in the same reaction system.
Article
Engineering, Environmental
Xiaoyan Zhang, Yingying Guo, Guangliang Liu, Yanwei Liu, Jianbo Shi, Ligang Hu, Lixia Zhao, Yanbin Li, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: The widely distributed marine bacterium Alteromonas sp. KD01 is capable of extracellularly reducing Hg(II) through a superoxide-dependent mechanism. The generation of superoxide by Alteromonas sp. KD01 and the role of NADH in Hg(II) reduction were demonstrated. This finding provides insights into Hg(II) reduction by superoxide in various aerobic environments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)