Article
Chemistry, Multidisciplinary
Jialu Yao, Ke Huang, Dashuai Zhu, Tan Chen, Yufeng Jiang, Junyi Zhang, Lijie Mi, He Xuan, Shiqi Hu, Junlang Li, Yafeng Zhou, Ke Cheng
Summary: Myocardial infarction is the most common cause of death worldwide, with heart failure becoming a major determinant of adverse prognosis in survivors. Exosome spray (EXOS) based on MSC exosomes and biomaterials shows promise in improving heart function, reducing fibrosis, and promoting angiomyogenesis for heart repair, making it a feasible and safe strategy for future therapeutic delivery.
Article
Biophysics
Laszlo Kekedy-Nagy, James M. Perry, Samuel R. Little, Oriol Y. Llorens, Steve C. C. Shih
Summary: We have developed a new on-off, cost-effective, and rapid electrochemical aptasensor combined with a microfluidics cartridge system for the detection of Delta(9)-THC in human saliva. The sensor showed a high sensitivity to Delta(9)-THC and can detect it at a concentration as low as 1 nM in PBS. However, the presence of raw human saliva significantly increased the limit of detection due to interfering elements. With saliva filtration, the limit of detection improved to 5 nM, which is lower than the concentrations associated with impairment.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Hai Jiang, Zhikang Sun, Qian Guo, Xuan Weng
Summary: The thread-based microfluidic electrochemical aptasensor is designed for rapid and highly sensitive detection of Vibrio parahaemolyticus in seafood. It has higher detection sensitivity and shorter assay time compared to traditional methods.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Chemistry, Analytical
Yongqi Yu, Qunqun Guo, Wenli Jiang, Hui Zhang, Chenxin Cai
Summary: An AND logic gate assisted by dual aptamers was developed for sensitive electrochemical detection of tumor-derived sEVs based on a cyclic enzymatic signal amplification strategy. The detection for CCRF-CEM sEVs showed a good linear response with a potential application in accurate cancer diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Min Ni, Xinyue Wu, Yueyue Gui, Yue Huang, Feng Wang, Chao Li
Summary: Exosomes are emerging cancer biomarkers and this study presents a dual metal-organic framework (MOF)- based electrochemical biosensor for capturing and detecting cancerous exosomes. The biosensor consists of a pH-sensitive ZIF-8-engineered screen-printed carbon electrode for signal transduction, a pH-insensitive magnetic nanoparticle-modified copper-based MOF (MNP/Cu-BTC MOF) for exosome collection, and a DNA logic system for DNA computation.
ACS APPLIED NANO MATERIALS
(2023)
Article
Electrochemistry
Brenda M. de C. Costa, Sophie Griveau, Fethi Bedioui, Fanny d' Orlye, Jose Alberto F. da Silva, Anne Varenne
Summary: We demonstrate the concept of a reusable 3D printed separation device with integrated electrodes for electrochemical detection. The device was optimized for easy and low-cost fabrication and showed good repeatability and linear response in the tested concentration range.
ELECTROCHIMICA ACTA
(2022)
Article
Oncology
Elisabeth Rackles, Patricia Hernandez Lopez, Juan M. Falcon-Perez
Summary: The analysis of extracellular vesicles (EVs) as a source of cancer biomarkers is a promising field, providing low-invasive biomarkers. EVs, present in most body fluids and released by all cell types, including cancer cells, can serve as a potential source of reliable biomarkers for cancer. EV biomarkers in liquid biopsy can complement current medical technologies for cancer diagnosis, prognosis, and therapy monitoring, particularly for glioblastoma.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Chemistry, Physical
Costanza Culmone, Kirsten Lussenburg, Joost Alkemade, Gerwin Smit, Aimee Sakes, Paul Breedveld
Summary: Additive manufacturing technology offers benefits in the medical instruments field by reducing component count, enhancing functionalities, and enabling personalized customization. The concept of a fully 3D-printed handheld steerable instrument for laparoscopic surgery showcases the integration of ergonomic principles and additive manufacturing, resulting in improved control and comfort during surgical procedures.
Article
Biochemistry & Molecular Biology
Yu Xing, Juntao Liu, Shuai Sun, Tao Ming, Yang Wang, Jinping Luo, Guihua Xiao, Xinrong Li, Jingyu Xie, Xinxia Cai
Summary: A paper-based microfluidic aptasensor was developed for label-free electrochemical detection of PD-L1 in liquids, showing good performance such as low cost, portability, and high sensitivity.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Antonios Keirouz, Yasemin L. L. Mustafa, Joseph G. G. Turner, Emily Lay, Ute Jungwirth, Frank Marken, Hannah S. S. Leese
Summary: Conductive polymeric microneedle arrays have the potential to be used as minimally invasive monitoring tools in biodevices and wearables. However, previous efforts on microneedle electrodes have been limited to metallic substrates, which lack biological stability and are costly to manufacture. In this study, additive manufacturing is used to fabricate acrylic-based microneedle devices, which are then coated with conductive polypyrrole and poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) films. These entirely polymer-based microneedle arrays act as dry conductive electrodes without the need for a metallic seed layer. Two coating methods, in situ polymerization and drop casting, are utilized to achieve conductivity. The fabricated microneedles penetrate porcine skin grafts without compromising their conductivity or morphology, and the coatings demonstrate durability over multiple penetration cycles. The non-cytotoxic nature of the conductive microneedles is confirmed using human fibroblast cells. This fabrication strategy offers a promising approach to manufacturing polymer-based conductive microneedle surfaces for biosensing applications.
Article
Chemistry, Analytical
Ayemeh Bagheri Hashkavayi, Byung Seok Cha, Eun Sung Lee, Ki Soo Park
Summary: Breast cancer is a leading cause of cancer-related death, and an effective diagnostic system for early cancer detection is required. In this study, an ultrasensitive electrochemical aptasensor based on metal ion signals was developed for the multiplex detection of exosome biomarkers. The sensor can simultaneously and specifically detect EpCAM and HER-2 positive exosomes with a low detection limit.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Yuanyuan Zou, Zhengkang Chu, Jiuchuan Guo, Shan Liu, Xing Ma, Jinhong Guo
Summary: Diabetes and its complications are a major threat to the health and well-being of millions of people. Continuous glucose monitoring (CGM) technology, particularly based on electrochemical sensing principles, has great potential to improve the quality of life of diabetics by overcoming the limitations of self-monitoring blood glucose (SMBG). However, the application of minimally invasive electrochemical CGM sensors is still limited due to issues such as invasiveness, short lifespan, biocompatibility, and calibration and prediction. Recent developments in materials and technologies have significantly improved the performance of these sensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Peng Zhao, Jilin Zheng, Yi Liang, Fengchun Tian, Lan Peng, Danqun Huo, Changjun Hou
Summary: The fabrication of an integrated microfluidic electrochemical platform allows for sensitive detection of cancer biomarker CEA. The platform shows superior performance with user-friendly operation and promising application prospects for precise determination of CEA in serum.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Analytical
John A. Tetteh, Elizabeth A. Hayter, R. Scott Martin, Istvan Z. Kiss
Summary: In this study, we investigated the kinetic features of complex charge transfer chemical reactions using a microelectrode integrated in a flow cell made with 3D printing technology. Through experimentation and numerical simulation, we discovered new phenomena and conditions, and compared them with traditional approaches.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Aneesh Koyappayil, Sachin Ganpat Chavan, Mohsen Mohammadniaei, Anna Go, Sei Young Hwang, Min-Ho Lee
Article
Biophysics
Mohsen Mohammadniaei, Aneesh Koyappayil, Yi Sun, Junhong Min, Min-Ho Lee
BIOSENSORS & BIOELECTRONICS
(2020)
Review
Chemistry, Analytical
Maryam Naseri, Mohsen Mohammadniaei, Yi Sun, Jon Ashley
Article
Engineering, Biomedical
Ming Zhang, Wentao Wang, Fan Wu, Tao Zheng, Jon Ashley, Mohsen Mohammadniaei, Qicheng Zhang, Mingqian Wang, Li Li, Jian Shen, Yi Sun
Review
Biotechnology & Applied Microbiology
Changhong Zhao, Xuebin Song, Ya Liu, Yifeng Fu, Lilei Ye, Nan Wang, Fan Wang, Lu Li, Mohsen Mohammadniaei, Ming Zhang, Qiqing Zhang, Johan Liu
JOURNAL OF NANOBIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jon Ashley, Anna-Lisa Schaap-Johansen, Mohsen Mohammadniaei, Maryam Naseri, Paolo Marcatili, Marta Prado, Yi Sun
Summary: The TdT enzyme plays a crucial role in V(D)J recombination in lymphocytes, allowing for diverse expression of immunoglobulins and T-cell receptors. A new screening method was demonstrated using TdT to form vsDNA libraries for binding to protein targets, offering the possibility of rational aptamer design and controlled reaction conditions for improved screening outcomes. This approach allows for screening based on both size and sequence in a single round, reducing PCR bias and resulting in high affinity and selective aptamers.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Food Science & Technology
Subhankar Mukherjee, Soumyadeb Bhattacharyya, Koustuv Ghosh, Souvik Pal, Arnab Halder, Maryam Naseri, Mohsen Mohammadniaei, Subrata Sarkar, Alokesh Ghosh, Yi Sun, Nabarun Bhattacharyya
Summary: In recent decades, heavy metal contamination has adversely affected the environment and human health. Intensive research and development have been conducted in heavy metal detection technologies, including laboratory-based analytical instruments and innovative sensor devices.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ming Zhang, Wentao Wang, Mohsen Mohammadniaei, Tao Zheng, Qicheng Zhang, Jon Ashley, Shunjie Liu, Yi Sun, Ben Zhong Tang
Summary: The study introduces a new type of nanoparticles, BK@AIE NPs, which can enhance transportation and accumulation inside tumors by activating BTB adenosine receptors. These nanoparticles can be used in photothermal therapy to treat deep-seated tumors and inhibit tumor progression, resulting in an extended survival span for mice.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Ming Zhang, Xuefeng Jiang, Qicheng Zhang, Tao Zheng, Mohsen Mohammadniaei, Wentao Wang, Jian Shen, Yi Sun
Summary: The study introduces a novel strategy using multifunctional nanoparticles for synergistic immunotherapy, which enhances intratumoral infiltration of lymphocytes and sensitizes tumors to PDL1 blockade therapy through photodynamic therapy. This approach effectively suppresses GBM growth in mouse models.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Applied
Wentao Wang, Qicheng Zhang, Ming Zhang, Xintong Lv, Zihan Li, Mohsen Mohammadniaei, Ninglin Zhou, Yi Sun
Summary: A novel therapeutic nanocomposite was developed for wound bacterial infections and tumor recurrence, utilizing released reactive oxygen species to kill bacteria and inhibit tumor cell regeneration, as well as utilizing photothermal properties to penetrate the blood tumor barrier for brain tumor treatment. The combination of the nanocomposite with immunotherapy showed potential for improving postoperative therapy and multi-tumor treatments.
CARBOHYDRATE POLYMERS
(2021)
Article
Electrochemistry
Maryam Naseri, Arnab Halder, Mohsen Mohammadniaei, Marta Prado, Jon Ashley, Yi Sun
Summary: This study introduces a novel electrochemical aptasensor with a multivalent aptamer immobilized on the electrode surface for label-free detection of lactoferrin with high sensitivity and wide detection range. The biosensor shows excellent selectivity and reproducibility in buffer solution, and is successfully applied for quantifying lactoferrin concentrations in urine samples.
ELECTROCHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Mohsen Mohammadniaei, Ming Zhang, Jon Ashley, Ulf Bech Christensen, Lennart Jan Friis-Hansen, Rasmus Gregersen, Jan Gorm Lisby, Thomas Lars Benfield, Finn Erland Nielsen, Jens Henning Rasmussen, Ellen Botker Pedersen, Anne Christine Rye Olinger, Laerke Torring Kolding, Maryam Naseri, Tao Zheng, Wentao Wang, Jan Gorodkin, Yi Sun
Summary: The study introduces a novel non-enzymatic isothermal amplification method for rapid detection of SARS-CoV-2 RNA, without the need for reverse transcription, with high specificity and sensitivity. The method is simple, fast, cost-effective, stable, and suitable for a wide range of testing needs.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Yutian Su, Fan Wu, Qiuxian Song, Mengjie Wu, Mohsen Mohammadniaei, Taiwei Zhang, Baolei Liu, Shishan Wu, Ming Zhang, Ao Li, Jian Shen
Summary: Nanozyme-based catalytic therapy plays an important role in tumor therapy by generating lethal reactive oxygen species. However, most nanozymes have mono catalytic performances in the tumor microenvironment, which lowers their therapeutic efficiency. In this study, a newly-developed nanozyme with dual enzyme-mimic catalytic activity is designed, which can achieve an enhanced nanocatalytic therapy effect through photothermal augmentation.
Article
Chemistry, Multidisciplinary
Tao Zheng, Wentao Wang, Mohsen Mohammadniaei, Jon Ashley, Ming Zhang, Ninglin Zhou, Jian Shen, Yi Sun
Summary: This study developed a targeted delivery system for miRNA-21 using ligand-modified nanoparticles, which temporarily opened the blood-tumor barrier to achieve effective transportation and controlled release in glioblastoma treatment, leading to the inhibition of tumor growth and angiogenesis.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)