Article
Chemistry, Analytical
Jiang Li, Lingli Qu, Huitong Li, Lu Zhao, Tongdan Chen, Jiaying Liu, Yuting Gao, Hongzhi Pan
Summary: The excessive use of antibiotics has led to water contamination and posed threats to human health. This study successfully synthesized a hollow PCN-222 MOF with a large surface area and abundant functional groups, particularly the porphyrin cores. Gold particles were incorporated into the porphyrin core to enhance the electrical conductivity of the hollow PCN-222. This modified composite demonstrated exceptional efficacy in immobilizing the aptamer and achieved the detection of chloramphenicol with high selectivity and reproducibility.
Article
Chemistry, Multidisciplinary
Yu Xing, Juntao Liu, Jinping Luo, Tao Ming, Gucheng Yang, Shuai Sun, Shengwei Xu, Xinrong Li, Enhui He, Fanli Kong, Shi Yan, Yue Yang, Xinxia Cai
Summary: In this study, a dual-channel electrochemical platform was designed and fabricated for rapid detection of the important proteins PD-1 and PD-L1 in cancer immunotherapy. The platform utilized nano-composites and specific antibodies, demonstrating high detection sensitivity and range. This provides a low-cost and portable method for detection.
Article
Chemistry, Analytical
Sanaz Akbarzadeh, Habibollah Khajehsharifi, Saeedeh Hajihosseini
Summary: A novel, rapid, and label-free aptamer-based electrochemical biosensor was designed for the detection of oxytetracycline (OTC), utilizing a newly synthesized nanocomposite to enhance electrical conductivity of the electrode surface. The aptasensor showed high sensitivity, low detection limit, wide linear range, and good repeatability for measuring OTC content in milk samples. The results demonstrated the effectiveness of the aptasensor in real sample analysis.
Article
Chemistry, Analytical
Xueying Yang, Wenjuan Guo, Ahmad Umar, Hassan Algadi, Ahmed A. Ibrahim, Chengxian Zhao, Zhe Ren, Luyan Wang, Meishan Pei
Summary: A highly sensitive electrochemical aptasensor based on gold nanoparticle decorated Ti3C2Tx nanocomposites was developed for the detection of chloramphenicol. The prepared composite exhibited high surface area, good conductivity, and thermal stability, and provided abundant binding sites for aptamers, resulting in high sensitivity and selectivity for chloramphenicol determination. The aptasensor showed a low detection limit of 13.18 fg mL(-1), a linear range of 1 to 700 pg mL(-1), and excellent reproducibility, stability, and selectivity. Real milk samples were successfully analyzed with recoveries ranging from 98.93% to 101.93%.
Article
Chemistry, Analytical
Cem Erkmen, Gozde Aydogdu Tig, Bengi Uslu
Summary: A sandwich-type electrochemical aptasensor was developed for sensitive detection of leptin in biological samples. The synergistic effect of zinc oxide and gold nanoparticles improved the electrocatalytic activity, allowing for a wide linear concentration range and a low detection limit. The aptasensor demonstrated good selectivity, reproducibility, and stability, making it suitable for practical applications.
Article
Chemistry, Analytical
Yue Zhang, Shuyi Chen, Jie Ma, Xiaobin Zhou, Xinchen Sun, Hongyun Jing, Mei Lin, Chenglin Zhou
Summary: This study presents a novel enzyme-catalyzed electrochemical aptasensing platform for highly specific and ultrasensitive detection of sPD-L1, a protein involved in breast cancer recurrence and metastasis. The developed method demonstrates high sensitivity and a wide detection range, offering potential for early diagnosis and disease surveillance of breast cancer.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Reza Zaimbashi, Somayeh Tajik, Hadi Beitollahi, Masoud Torkzadeh-Mahani
Summary: An electrochemical aptasensor was developed to detect the amino acid homocysteine (HMC) using a high-specificity HMC aptamer. The sensor was fabricated by immobilizing the aptamer on an Au nanostructured/carbon paste electrode (Au-NS/CPE) with high affinity to HMC. The aptasensor displayed high specificity as no clear current alteration was observed in the presence of common interferants. It successfully sensed HMC in a range of 0.1-30 μM with a low limit of detection (LOD) of 0.03 μM.
Review
Immunology
Lin Zhang, Bo Hao, Zhihua Geng, Qing Geng
Summary: Toripalimab, a selective anti-PD-1 monoclonal antibody, has shown anti-tumor effects in melanoma, nasopharyngeal carcinoma, and urothelial carcinoma, and could be a valuable choice for future tumor treatment decision-making.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Taisuke Kaiho, Hidemi Suzuki, Atsushi Hata, Hiroki Matsumoto, Kazuhisa Tanaka, Yuichi Sakairi, Shinichiro Motohashi, Ichiro Yoshino
Summary: Immune checkpoint molecules, such as PD-1 and PD-L1, have been shown to be important in lung cancer treatment. This study explored the role of these proteins in acute rejection in a mouse model of tracheal transplantation and found that PD-L1 Fc recombinant protein could decrease inflammatory cytokines and reduce the proportion of CD4+ T cells, suggesting a potential novel target for immunotherapy in lung transplantation.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andrzej Kowalski, Katarzyna Malinowska, Jurek Olszewski, Hanna Zielinska-Blizniewska
Summary: The interaction of PD-1 and PD-L1 in laryngeal tumors allows cancer cells to escape from the immune system. Gene expression of PD-1 and PD-L1 was significantly higher in tumor tissue compared to unchanged mucosa, with higher expression levels associated with larger tumor sizes. These findings suggest potential for targeted immunotherapy with anti-PD1 and anti-PD-L1 monoclonal antibodies in treating laryngeal tumors.
Article
Nanoscience & Nanotechnology
Guiyin Li, HaiMei Li, Wei Chen, Huijiang Chen, Guanxiong Wu, Mingxiong Tan, Jintao Liang, Zhide Zhou
Summary: By combining different nanoparticles, an electrochemical aptasensor was developed for quantitatively detecting GPC3. The sensor showed a linear response range and a low detection limit, making it promising for clinical diagnosis of hepatocellular carcinoma.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Clinical Neurology
Christopher M. Jackson, John Choi, Denis Routkevitch, Ayush Pant, Laura Saleh, Xiaobu Ye, Justin M. Caplan, Judy Huang, Cameron G. McDougall, Drew M. Pardoll, Henry Brem, Rafael J. Tamargo, Michael Lim
Summary: The study aimed to evaluate the role of programmed death-1 (PD-1) in cerebral vasospasm using a mouse model. Results showed that PD-1+ monocytes were identified as mediators of cerebral vasospasm, supporting PD-1 agonism as a novel therapeutic strategy. Human correlative studies confirmed the presence of PD-1+ monocytes in the peripheral blood of patients with ruptured aneurysms.
Review
Oncology
Chia-Jung Li, Li-Te Lin, Ming-Feng Hou, Pei-Yi Chu
Summary: Significant progress has been made in understanding the role of PD-L1 in breast cancer, especially in TNBC. Early clinical trials of PD-L1/PD-1 inhibitors have shown efficacy in refractory metastatic breast cancer patients, particularly in TNBC. Mechanisms and factors influencing the immunoediting process have been summarized and analyzed in detail.
Article
Immunology
Suxia Geng, Ruohao Xu, Xin Huang, Minming Li, Chengxin Deng, Peilong Lai, Yulian Wang, Ping Wu, Xiaomei Chen, Jianyu Weng, Xin Du
Summary: PD-1 expression significantly higher in high-risk MDS patients, and its increase during initial HMA treatment cycles may serve as an independent negative prognostic factor predicting AML transformation and survival. Monitoring PD-1 expression levels during HMA treatment cycles could help identify patients who may benefit from combined therapy with HMA and PD-1 inhibitors.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Huizhen Cao, Tianyu Wu, Xue Zhou, Shuyang Xie, Hongfang Sun, Yunxiao Sun, Youjie Li
Summary: Leukemia cells induce immunosuppression of the bone marrow microenvironment, preventing the immune system from clearing tumor cells. The PD-1/PD-L1 axis acts as a significant mechanism for tumor cells to evade immune surveillance. The development of drugs targeting PD-1/PD-L1 in leukemia is still at the clinical-trial stage.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)