Review
Engineering, Biomedical
Preety Rawal, Dinesh M. Tripathi, Seeram Ramakrishna, Savneet Kaur
Summary: Three-dimensional bioprinting technology is emerging to address limitations in current organoid technologies, showing success in printing stem cells and organoids. Vascularization strategies for bioprinted organoids are critical for achieving complex tissue organization. Harnessing and directing advancements in 3D bioprinting is essential for realizing the translational applications of organoids in disease modeling and regenerative medicine.
BIO-DESIGN AND MANUFACTURING
(2021)
Review
Pharmacology & Pharmacy
Maritza Londono-Berrio, Cristina Castro, Ana Canas, Isabel Ortiz, Marlon Osorio
Summary: Tumor organoids, three-dimensional assemblies of heterogeneous cells derived from patient samples, have emerged as an ideal model for evaluating cancer therapies due to their ability to mimic the key characteristics of original tumors. However, there is limited research on the techniques and material development of tumor organoids, as well as the physicochemical and biological properties of materials that biomimic the tumor extracellular matrix. Additionally, tools to identify the correspondence between native tumors and tumor organoids are scarce. Despite these challenges, efforts have been made to standardize the process of obtaining organoids that resemble patient-derived tumor tissue. Organoids have shown promise in drug evaluation for various types of tumors, and their use is expected to increase in clinical practice for studying cancer.
Review
Biotechnology & Applied Microbiology
Sara Abdollahi
Summary: Initially considered as junk carriers, extracellular vesicles (EVs) have been found to play a crucial role in cell communication by transporting RNA, DNA, and other bioactive components. Recent studies are utilizing 3D cell culture models to analyze EVs in physiological and pathological contexts, although standard and reproducible methods for EV analysis in these models are lacking.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Gianmarco Concilia, Austin Lai, Peter Thurgood, Elena Pirogova, Sara Baratchi, Khashayar Khoshmanesh
Summary: The study utilizes a unique dynamic gravity pump to generate transient flows in microfluidic systems for investigating mechanotransduction of shear stress in sensitive cells. Customized flow patterns can be generated by adjusting the attributes of the cam. The pump features controllable, simple, low-cost, compact, and modular design, making it suitable for studying shear sensitive cells under transient flows.
Review
Chemistry, Physical
Bauer L. LeSavage, Riley A. Suhar, Nicolas Broguiere, Matthias P. Lutolf, Sarah C. Heilshorn
Summary: The translation discusses how organotypic models of patient-specific tumors are changing our understanding of cancer heterogeneity and personalized medicine. These models can preserve genetic, proteomic, morphological, and pharmacotypic features of the parent tumor in vitro, and offer genomic and environmental manipulation. However, current cancer organoid culture techniques are uncontrolled and irreproducible, hindering the acceleration of insights into patient care.
Article
Chemistry, Multidisciplinary
Daniel R. Hunt, Katarina C. Klett, Shamik Mascharak, Huiyuan Y. Wang, Diana Gong, Junzhe Lou, Xingnan Li, Pamela C. Cai, Riley A. Suhar, Julia Y. Co, Bauer L. LeSavage, Abbygail A. Foster, Yuan Guan, Manuel R. Amieva, Gary Peltz, Yan Xia, Calvin J. Kuo, Sarah C. Heilshorn
Summary: The designer matrix hyaluronan elastin-like protein (HELP) enables the formation, differentiation, and passaging of adult primary tissue-derived intestinal organoids, supporting the proliferation and formation of enteroids with polarized internal lumens. This material allows for fundamental studies of organoid-matrix interactions and potential patient-specific optimization, offering a promising in vitro model for understanding intestinal development and disease in humans.
Article
Chemistry, Multidisciplinary
Lin Bao, Xuejing Cui, Xiaoyu Wang, Junguang Wu, Mengyu Guo, Na Yan, Chunying Chen
Summary: Engineered carbon nanotubes have a beneficial effect on the intestinal organoid system, promoting development through modulation of extracellular matrix viscoelasticity and intracellular energy metabolism. They also alter the metabolic profile of intestinal organoids, leading to increased mitochondrial activity, respiration, and nutrient absorption. The promotional effect of carbon nanotubes is dependent on the number of graphene layers, with multiwalled CNTs showing greater efficacy than single-walled CNTs.
Article
Biochemistry & Molecular Biology
Muxin Yue, Yunsong Liu, Ping Zhang, Zheng Li, Yongsheng Zhou
Summary: The study investigates the interaction between stem cell transcriptional and metabolic signals in extracellular matrices with different stiffnesses. It reveals that the stiffness of the matrix plays a role in determining the fate of bone marrow mesenchymal stem cells, with different stiffnesses leading to different differentiation pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Dongzhi Wang, Yibing Guo, Jiacheng Zhu, Fang Liu, Yan Xue, Yan Huang, Biwen Zhu, Di Wu, Haopeng Pan, Tiancheng Gong, Yuhua Lu, Yumin Yang, Zhiwei Wang
Summary: In this study, a 3D-printed islet organoid was developed by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA), which improved the efficacy of islet transplantation. The 3D-printed islet maintained cell adhesion and morphology, enhancing islet function and activity. The designed structure also promoted the formation of new blood vessels and the construction of islet organoids.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Jin Won Kim, Sun Ah Nam, Jawoon Yi, Jae Yun Kim, Jong Young Lee, Seo-Yeon Park, Tugce Sen, Yoo-mi Choi, Jae Yeon Lee, Hong Lim Kim, Hyung Wook Kim, Jiwhan Park, Dong-Woo Cho, Yong Kyun Kim
Summary: Using a kidney decellularized extracellular matrix hydrogel to culture hPSC-derived kidney organoids can promote vascularization and maturation of the organoids, leading to a pattern more similar to human kidneys.
Article
Biochemistry & Molecular Biology
Serena Calamaio, Marialaura Serzanti, Jennifer Boniotti, Annamaria Fra, Emirena Garrafa, Manuela Cominelli, Rosanna Verardi, Pietro Luigi Poliani, Silvia Dotti, Riccardo Villa, Giovanna Mazzoleni, Patrizia Dell'Era, Nathalie Steimberg
Summary: The process of developing a new drug is time-consuming and expensive, and hepatotoxicity is a major concern. Animal models have limitations due to interspecies differences, but human induced pluripotent stem cells (hiPSCs) offer new possibilities for drug testing. We miniaturized the process of generating hepatic organoids using hiPSC-derived mesenchymal stem cells, hepatocytes, and endothelial cells. These organoids were cultivated in vitro using static and dynamic cultures, and could be maintained for up to 1 week. This hiPSC-derived liver organoid model could potentially replace animal testing in pharmacological and toxicological studies.
Article
Engineering, Biomedical
Can Wang, Qiannan Zhao, Xiaohong Zheng, Shenglan Li, Jinyi Chen, Hanyun Zhao, Feng Chen, Lei Cui, Wenbin Li
Summary: Decellularized extracellular matrix (dECM) is used to generate three-dimensional in vitro tumor models that recapitulate tumor-ECM interactions. We developed a decellularized brain extracellular matrix slice (dBECMS) glioblastoma (GBM) culture model, which accurately recapitulated the transcriptional profile of GBM and enhanced GBM cell resistance to temozolomide. We also demonstrated the feasibility of the dBECMS-GBM model as a platform for drug response modeling.
ACTA BIOMATERIALIA
(2023)
Article
Gastroenterology & Hepatology
Jie Hong, Fangfang Guo, Shi-Yuan Lu, Chaoqin Shen, Dan Ma, Xinyu Zhang, Yile Xie, Tingting Yan, TaChung Yu, Tiantian Sun, Yun Qian, Ming Zhong, Jinxian Chen, Yanshen Peng, Cheng Wang, Xiang Zhou, Jianjun Liu, Qiang Liu, Xiong Ma, Ying-Xuan Chen, Haoyan Chen, Jing-Yuan Fang
Summary: The study revealed a positive correlation between Fusobacterium nucleatum and high glucose metabolism in patients with colorectal cancer (CRC), suggesting a potential role of gut microbiota in CRC development. Mechanistically, Fusobacterium nucleatum activated the lncRNA ENO1-IT1 pathway to regulate CRC cell glucose metabolism and biological functions. Targeting the ENO1 pathway may have therapeutic implications for CRC patients with elevated Fusobacterium nucleatum levels.
Article
Oncology
Yanhua Fang, Shanshan Liang, Jianong Gao, Zhe Wang, Cheng Li, Ruoyu Wang, Weiting Yu
Summary: This study compares the tumor biological behaviors of nasopharyngeal carcinoma (NPC) cells in 2D and 3D systems and investigates the correlation between radioresistance and extracellular matrix (ECM) stiffness. The results show that NPC cells cultured in 3D systems have a morphology more similar to in vivo tumor cells and exhibit stronger radioresistance. Additionally, NPC cells cultured in softer ECM systems show significant resistance to ionizing radiation.
CANCER CELL INTERNATIONAL
(2022)
Review
Oncology
Heming Ge, Mengxiang Tian, Qian Pei, Fengbo Tan, Haiping Pei
Summary: Recent in-depth studies have highlighted the significant role of extracellular matrix stiffness in various cellular processes such as growth, proliferation, migration, and immunity. Understanding the relationship between extracellular matrix stiffness and metabolism could lead to the discovery of novel therapeutic targets and inform the development of clinical approaches against a range of diseases.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
M. T. Ciubuc-Batcu, N. J. C. Stapelberg, J. P. Headrick, G. M. C. Renshaw
Summary: The nervous system relies on mitochondria, and impaired mitochondrial function is associated with major depressive disorder. Modulating mitochondrial function may be a therapeutic target for treating MDD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Correction
Biochemistry & Molecular Biology
Saowaluk Saisomboon, Ryusho Kariya, Piyanard Boonnate, Kanlayanee Sawanyawisuth, Ubon Cha'on, Vor Luvira, Yaovalux Chamgramol, Chawalit Pairojkul, Wunchana Seubwai, Atit Silsirivanit, Sopit Wongkham, Seiji Okada, Sarawut Jitrapakdee, Kulthida Vaeteewoottacharn
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Pavan Thapak, Zhe Ying, Victoria Palafox-Sanchez, Guanglin Zhang, Xia Yang, Fernando Gomez-Pinilla
Summary: Traumatic brain injury (TBI) impairs cellular energy demand, compromising neuronal function and plasticity. This study demonstrates that the mitochondrial activator humanin (HN) can counteract the reduction in mitochondrial bioenergetics caused by TBI, restore memory function and synaptic protein levels, and suppress inflammation and astrocyte proliferation. HN plays an integral role in normalizing fundamental aspects of TBI pathology.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Paul Murphy, Valeria A. Buzinova, Carrie E. Johnson
Summary: Progress has been made in the treatment of Alzheimer's disease through the development of anti-A beta therapeutics, which have shown modest efficacy in slowing the progression of the disease. However, the puzzling issue remains as to why completely removing A beta does not fully stop the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yang Zhang, Mengqiu Hao, Xuyang Yang, Su Zhang, Junhong Han, Ziqiang Wang, Hai-Ning Chen
Summary: Colorectal cancer often requires adjuvant therapies to reduce tumor burden, and the efficacy of these therapies is significantly influenced by reactive oxygen species (ROS). ROS-mediated colorectal cancer adjuvant therapies involve multiple mechanisms, and preliminary clinical trials have shown the potential of ROS-manipulating therapy in enhancing treatment outcomes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Mengxin Li, Xuanzhong Wang, Xuyang Chen, Jinghui Hong, Ye Du, Dong Song
Summary: Pancreatic adenocarcinoma (PAAD) is a common digestive malignant tumor with limited treatment options. This study demonstrates that TGM2 may serve as a marker for treatment and prognosis in pancreatic cancer patients. Co-treatment of low dose cisplatin (DDP) and the TGM2 inhibitor GK921 effectively inhibits PAAD cell viability and proliferation in vitro and in vivo, by inhibiting epithelial-to-mesenchymal transition (EMT) induced by TGM2 and enhancing cell cycle arrest and apoptosis caused by DDP. These findings suggest that the combination of GK921 and DDP holds promise as a treatment for PAAD patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Liaoran Niu, Qi Wang, Fan Feng, Wanli Yang, Zhenyu Xie, Gaozan Zheng, Wei Zhou, Lili Duan, Kunli Du, Yiding Li, Ye Tian, Junfeng Chen, Qibin Xie, Aqiang Fan, Hanjun Dan, Jinqiang Liu, Daiming Fan, Liu Hong, Jian Zhang, Jianyong Zheng
Summary: This review provides a comprehensive summary of the interaction between cancer cells and macrophages in the tumor microenvironment, and discusses the role of small extracellular vesicles (sEVs) in this process. It also explores the various effects of macrophage-secreted sEVs on tumor malignant transformation, and addresses the therapeutic advancements and challenges associated with these vesicles.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Neha Sawant, Sudhir Kshirsagar, P. Hemachandra Reddy, Arubala P. Reddy
Summary: Depression is a common neuropsychiatric comorbidity in Alzheimer's disease (AD) and other Tauopathies. Selective serotonin reuptake inhibitor (SSRI) treatment, such as Citalopram, not only has anti-depressive and anxiolytic effects, but also helps improve neurogenesis, reduce amyloid burden & Tau pathologies, and neuroinflammation in AD. In this study, Citalopram was found to reduce pathologically pTau level, increase synaptic gene expression and cytoskeletal structure, as well as improve cell survival, mitochondrial respiration, and mitochondrial morphology in cells expressing mutant APP and Tau. These findings suggest that Citalopram could be a promising therapeutic drug for treating depression and AD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Chen, Jiulin Tan, Chuan Yang, Zhiguo Ling, Jianzhong Xu, Dong Sun, Fei Luo
Summary: Bone is a self-healing organ that undergoes continuous regeneration through the cooperation of osteoclasts and osteoblasts. This study used ATAC-seq and RNA-Seq techniques to investigate the chromatin accessibility and transcriptomic landscape of osteoblast differentiation and mineralization. The results showed that global chromatin accessibility was extensively improved during osteoblastogenesis. Additionally, several transcription factors including MEF2A, PRRX1, Shox2, and HOXB13 were found to modulate the promoter accessibility of target genes during osteoblast differentiation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Zi-Ran Kang, Shanshan Jiang, Ji-Xuan Han, Yaqi Gao, Yile Xie, Jinxian Chen, Qiang Liu, Jun Yu, Xin Zhao, Jie Hong, Haoyan Chen, Ying-Xuan Chen, Huimin Chen, Jing-Yuan Fang
Summary: The study demonstrates that BCAA metabolism is involved in the development of colorectal cancer (CRC). BCAT2 deficiency promotes CRC progression by inhibiting BCAA metabolism and chronically activating the mTORC1 pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Chao Zheng, Lingling Liu, Caiyun Liu, Fengna Chu, Yue Lang, Shan Liu, Yan Mi, Jie Zhu, Tao Jin
Summary: Inducing tolerogenic dendritic cells (tDCs) with low RelB expression could effectively alleviate symptoms and reduce immune cell infiltration and demyelination in experimental autoimmune encephalomyelitis (EAE) mouse model.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Hang Lam Li, Simei Go, Jung-Chin Chang, Arthur Verhoeven, Ronald Oude Elferink
Summary: This review highlights the distinct characteristics and crucial role of soluble adenylyl cyclase (sAC) in cellular processes, as well as recent significant advancements in the field of sAC research.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Seco-Cervera, D. Ortiz-Masia, D. C. Macias-Ceja, S. Coll, L. Gisbert-Ferrandiz, J. Cosin-Roger, C. Bauset, M. Ortega, B. Heras-Moran, F. Navarro-Vicente, M. Millan, J. V. Esplugues, S. Calatayud, M. D. Barrachina
Summary: The study revealed the presence of resistance to apoptosis in complicated ileal Crohn's disease, with PDGFB inducing an ETS1-mediated resistance to apoptosis associated with an inflammatory and fibrogenic pattern of expression in intestinal fibroblasts. Potential targets against ileal fibrosis include PDGFRB, IL1R1, or MCL1.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yunmeng Wang, Ping Cheng
Summary: Oncolytic viruses (OVs) are emerging as therapeutically relevant anticancer agents, especially when combined with genetically modified bispecific T cell engagers (BiTEs). This combination strategy can overcome the limitations of BiTEs alone and provide targeted cytotoxicity to solid tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Stephanie Tannous, Hassan Y. Naim
Summary: Congenital sucrase-isomaltase deficiency (CSID) is an autosomal recessive disorder caused by variants in the SI gene. A frameshift mutation called c.273_274delAG (p.Gly92Leufs*8) has been identified in CSID patients in Greenlandic population, which leads to loss of digestive function of SI. Surprisingly, the truncated mutant can still be located on the cell surface and interacts with wild type SI, negatively affecting its enzymatic function. Furthermore, heterozygote carriers of this mutation may also exhibit CSID symptoms.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)