Article
Fisheries
Xianliang Zhao, Dong Yang, Mingqi Zhong, Zhongyang Lin, Defu Yao, Yueling Zhang
Summary: In this study, the researchers identified the outer membrane protein LamB of V. parahaemolyticus and its interaction with shrimp hemocyanin. They also found that LamB could down-regulate the expression of Toll2, LGBP, and hemocyanin, and increase bacterial loading in shrimp hemolymph.
Article
Microbiology
Haipeng Guo, Xuezhi Fu, Jikun He, Ruoyu Wang, Mengchen Yan, Jing Wang, Pengsheng Dong, Lei Huang, Demin Zhang
Summary: This study reveals the important role of gut bacterial community induced by a biofloc system (BFS) in protecting shrimp from bacterial pathogens. The study also provides a micro-ecological regulation strategy to improve aquatic animal diseases.
Article
Biochemistry & Molecular Biology
Chuanqi Wang, Defu Yao, Mingming Zhao, Kaiyu Lu, Zhongyang Lin, Xiuli Chen, Yongzhen Zhao, Yueling Zhang
Summary: This study found that Vp (AHPND) infection in cultured shrimp increased the expression of lipid droplet protein LvPerilipin and promoted the formation of lipid droplets. Knockdown of LvPerilipin increased shrimp survival rate and inhibited Vp (AHPND) proliferation. Furthermore, depletion of LvPerilipin increased the production of reactive oxygen species, which may be responsible for the decreased Vp (AHPND) proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Shuang Liu, Wei Wang, Tianchang Jia, Lusheng Xin, Ting-ting Xu, Chong Wang, Guosi Xie, Kun Luo, Jun Li, Jie Kong, Qingli Zhang
Summary: This study identified the key virulence factor VHVP-2 of Vibrio parahaemolyticus (Vp (TPD)) that causes translucent post-larvae disease (TPD) in shrimp. The findings shed light on the pathogenic mechanism of Vp (TPD) and contribute to the development of early diagnostic strategies for TPD in shrimp hatcheries.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
Thao Nguyen, Andrea Alfaro, Bonny Bayot Arroyo, Jenny Antonia Rodriguez Leon, Stanislaus Sonnenholzner
Summary: The study used a metabolomics approach to measure molecular responses of shrimp exposed to Vibrio parahaemolyticus, revealing an increase in metabolic rate, elevated TCA cycle, and gluconeogenesis activities in infected shrimp. This provides important new findings about the pathogenic mechanisms of V. parahaemolyticus invasion in shrimp at the metabolic level.
Article
Fisheries
Xue Zhang, Jingfeng Sun, Zhuoran Han, Feng Chen, Aijun Lv, Xiucai Hu, Xueliang Sun, Hongli Qi, Yongjun Guo
Summary: Homeostasis of intestinal microbiota is crucial to host health, and pathogen invasion leads to dynamic changes in gut microbes. Vibrio parahaemolyticus is an important pathogen of Pacific white shrimp, causing high mortality. The TJA114 strain lacks the pir gene but showed strong pathogenicity, altering bacterial composition in shrimp gut. Metabolites involved in various pathways were identified, providing new insights into vibriosis pathogenesis in shrimp.
Article
Biotechnology & Applied Microbiology
Qian Yang, Qingyao Wang, Junmin Wu, Yixiang Zhang, Dawei Wei, Baocheng Qu, Ying Liu, Songzhe Fu
Summary: The study showed that RAS farming systems maintained stable Vibrio parahaemolyticus population structure, while traditional farming systems exhibited annual replacement of populations. Whole-genome sequencing of 50 isolates divided them into five clusters, covering different genotypes.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Dunhua Zhang, Troy J. Bader, Miles D. Lange, Craig A. Shoemaker, Benjamin H. Beck
Summary: The study identified PirA and PirB as the major virulence factors causing AHPND and found that their interactions enhanced the toxic effect.
MICROBIAL PATHOGENESIS
(2021)
Article
Gastroenterology & Hepatology
Daniel Tchelet, Kinga Keppel, Eran Bosis, Dor Salomon
Summary: In this study, proteomics was used to identify antibacterial effectors in the secretome of Vibrio parahaemolyticus strains. The results revealed two conserved effectors and other effectors that are only present in subsets of strains, suggesting a core and accessory effector repertoire of the T6SS2 system. A conserved Rhs effector was also discovered to be required for T6SS2 activity.
Article
Environmental Sciences
Yunato Kuroyanagi, Jiro Tsuchiya, Chunqi Jiang, Sayaka Mino, Hisae Kasai, Daisuke Motooka, Tetsuya Iida, Masataka Satomi, Tomoo Sawabe
Summary: Light is an important stimulus for living organisms, but the effects of blue light on non-phototrophic prokaryotes are not well understood. This study investigated the molecular mechanisms of Vibrio parahaemolyticus cells in response to blue light exposure and found that it caused changes in reactive oxygen species and up-regulation of certain genes. Red and orange light had different effects on bacterial virulence and gene expression. These findings enhance our understanding of light responses in non-phototrophic marine prokaryotes.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Food Science & Technology
Vashti M. Campbell, Alexander Chouljenko, Steven G. Hall
Summary: Consuming raw oysters can increase health risks due to the concentration of potentially pathogenic bacteria such as Vibrio vulnificus and Vibrio parahaemolyticus. Depuration is a method that can reduce bacterial contamination in live oysters, but it is challenging to eliminate Vibrios completely.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Food Science & Technology
Xiangpeng Han, Qingying Chen, Xingguo Zhang, Xiaolan Chen, Dongsheng Luo, Qingping Zhong
Summary: This study found that Lactiplantibacillus plantarum 2057 can effectively inhibit the formation of Vibrio parahaemolyticus biofilm and interfere with its adhesion ability. In addition, the extract of L. plantarum Z057 significantly reduces the biofilm biomass, metabolic activity, and various important components of V. parahaemolyticus. These findings suggest that L. plantarum 2057 could be a biocontrol strategy alternative to chemical methods against foodborne pathogens.
Article
Immunology
Min-Young Jeon, Jee Eun Han, Dong Gwang Lee, Young-Lai Cho, Ju-Hong Jang, Jangwook Lee, Jong-Gil Park, Do Hyung Kwon, Seon Young Park, Wantae Kim, Kyunglee Lee, Ji Hyung Kim, Nam-Kyung Lee
Summary: The study developed a sandwich ELISA-based immunoassay for detecting PirAB(Vp) by utilizing single-chain variable fragment (scFv) antibodies selected from an antibody library. The developed immunoassay showed high affinities and could detect the toxin in AHPND-causing Vibrio parahaemolyticus strains, suggesting its potential use in diagnosing and determining virulence in the global shrimp culture industry.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Monchai Pumkaew, Thanut Taweephitakthai, Penpicha Satanwat, Patchari Yocawibun, Parichat Chumtong, Wiboonluk Pungrasmi, Sorawit Powtongsook
Summary: The study found that a residual ozone concentration (ROC) dosage of 0.3 mg L-1 effectively controlled Vibrio parahaemolyticus while minimizing negative effects on nitrifying bacteria activity, striking a balance between pathogen control and biofilter functionality in a recirculating aquaculture system.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Immunology
Mediha Yildirim-Aksoy, Rashida Eljack, Eric Peatman, Benjamin H. Beck
Summary: This study evaluated the changes in hematology, immunology, and biochemical values in the hemolymph of shrimp infected with Vibrio parahaemolyticus. The results showed that shrimp's immune response was altered, and the hemolymph parameters could be used to assess the health status of shrimp.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemical Research Methods
Watcharachai Meemetta, Jose A. Domingos, Ha Thanh Dong, Saengchan Senapin
JOURNAL OF VIROLOGICAL METHODS
(2020)
Article
Biotechnology & Applied Microbiology
A. Mangott, J. Nappi, A. Delli Paoli Carini, P. Goncalves, K. Hua, J. A. Domingos, R. de Nys, T. Thomas
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Fisheries
Quyen Q. T. Banh, Jose A. Domingos, Ricardo C. C. Pinto, Kien T. Nguyen, Dean R. Jerry
Summary: This study found that E-2 delivered through feed is more suitable than EE2 for inducing precocious feminization of barramundi, and it has a dose-dependent feminizing effect.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Jose A. Domingos, Julie A. Goldsbury, Giana Bastos Gomes, Brett G. Smith, Christopher Tomlinson, Tim Bade, Corey Sander, Justin Forrester, Dean R. Jerry
Summary: Barramundi, a commercially important tropical aquaculture species, shows adaptability to different salinities, but selective breeding programs should consider the impact of genotype by environment interactions. This study reveals weak to moderate re-ranking of genotypes for harvest growth traits in L. calcarifer farmed in marine and freshwater conditions, suggesting the importance of considering GxE interactions in breeding programs.
Article
Fisheries
Quyen Q. T. Banh, Jarrod L. Guppy, Jose A. Domingos, Alyssa M. Budd, Ricardo C. C. Pinto, Adrien F. Marc, Dean R. Jerry
Summary: Through the treatment with a higher dose of E2, 78% of the barramundi underwent complete sex change to females, showing downregulation of male-biased genes and upregulation of female-biased genes, accompanied by decreased DNA methylation in the female gene cyp19a1a compared to the lower dose treatment group.
Article
Genetics & Heredity
Jose A. Domingos, Xueyan Shen, Celestine Terence, Saengchan Senapin, Ha Thanh Dong, Marie R. Tan, Susan Gibson-Kueh, Dean R. Jerry
Summary: Marine farming of barramundi in Southeast Asia is currently affected by viral diseases, with scale drop disease virus and L. calcarifer herpes virus being detected in all sampled fish. Histopathology showed abnormal fat accumulation in the livers of sick fish, inflammation and necrosis in the kidneys, and severe necrosis in the spleen. RNA degradation in the kidney and spleen of sick fish indicated organ failure in advanced stages of scale drop disease.
FRONTIERS IN GENETICS
(2021)
Article
Biodiversity Conservation
Hin Hung Tsang, Jose A. Domingos, Jacob A. F. Westaway, Maximilian H. Y. Kam, Roger Huerlimann, Giana Bastos Gomes
Summary: The use of environmental DNA (eDNA) technology to monitor parasites in fish farms revealed positive associations between rainfall and temperature with the abundance of Cryptocaryon irritans, a ciliate parasite. Additionally, the bacterial genus Coraliomargarita showed a significant negative relationship with the parasite abundance in water, indicating its potential role in controlling ciliate parasites in marine fish farms.
Article
Fisheries
Celestine Terence, Jose A. Domingos, Joseph Angelo Uichanco, Yann Monteil, Loic Monteil, Emmanuel De Braux, Quyen Q. T. Banh, Susan Gibson-Kueh, Dean R. Jerry
Summary: The study in Singapore found that barramundi farmed in sea cages are undergoing precocious sex change, with a decrease in male individuals and an increase in females as early as 2-3 years old. This phenomenon challenges hatcheries in selecting and maintaining male fish for breeding purposes, and further investigation is needed to understand the causes of this early sex change.
AQUACULTURE REPORTS
(2021)
Article
Environmental Sciences
Nattakarn Kruatong, Soydoa Vinitnantharat, Hai The Pham, Pham Thai Giang, Nguyen Huu Nghia, Nutchanat Ninlaor, Kasame Thepnoo, Hsu Lei Myint, Pavinee Pattanachan, Rattikan Neamchan, Phuong Ha Vu, Siriwan Wongsod
Summary: The research explored the long-term relationships between climate parameters and coastal water quality near shrimp farms in Thailand and Northern Vietnam. It was found that aquaculture farming in Northern Vietnam is more vulnerable to climate impacts compared to Thailand, but Vietnamese farmers have a certain level of adaptability to climate variability.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2021)
Article
Fisheries
Edwin Ong Jun Kiat, Thiviya Nair, M. Sobana, Ng Tze Hann, Jose A. Domingos, Giana Bastos Gomes
Summary: Scale drop disease virus (SDDV) is an emerging virus in Asian countries that infects barramundi, resulting in huge economic losses in aquaculture industries. The traditional methods of diagnosing viral diseases in aquaculture are inefficient, while the environmental DNA (eDNA) approach offers an innovative way to detect specific pathogens before animals become infected. This study tested the feasibility of using eDNA to detect SDDV and found that ddPCR is a more efficient technique for low-level detection of SDDV in aquatic environments.
Article
Fisheries
Alyssa M. Budd, Jarrod L. Guppy, Valentin Thepot, Jose A. Domingos, Dean R. Jerry
Summary: This study reveals that temperature treatment has an effect on gonadal DNA methylation and primary sexual development in sequential hermaphroditic fish, particularly with a delayed effect on the gonads of juvenile barramundi.
Article
Fisheries
Zhi Weng Josiah Poon, Xueyan Shen, Joseph Angelo Uichanco, Celestine Terence, Shi Wei Gavin Chua, Jose A. Domingos
Summary: This study reveals the molecular mechanisms behind the enhancement of red coloration in Malabar snappers by supplementing feed with astaxanthin, and provides a foundation for breeding and aquaculture practices to produce fish with desired traits favored by consumers.
Article
Economics
Maria Teresa Medeiros Garcia, Jose Amilcar Neves Domingos
JOURNAL OF ECONOMIC STUDIES
(2020)