Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Biophysics
Xinxin Ke, Yangjing Ou, Yu Lin, Tao Hu
Summary: This study presents the first CRISPR/Cas12a-based chemiluminescence enhancement biosensor using HCR amplifying strategy for nucleic acids detection, showing high sensitivity and specificity, low-cost, and visual imaging advantages. The platform exhibits excellent sensitivity in detecting synthetic DNA targets, and achieves superior sensitivity and specificity in the practical application with HPV clinical samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Xinxin Ke, Yangjing Ou, Yu Lin, Tao Hu
Summary: In this study, a chemiluminescence enhancement biosensor based on CRISPR/Cas12a with HCR amplifying strategy was developed for nucleic acids detection. The biosensor showed high sensitivity and specificity, low cost, and visual imaging. The detection limit for synthetic DNA target was 3 pM. The biosensor also exhibited excellent sensitivity and specificity when applied to HPV clinical samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Hai-Yan Jia, Hong-Li Zhao, Ting Wang, Pin-Ru Chen, Bin-Cheng Yin, Bang-Ce Ye
Summary: A versatile and sensitive miRNA detection platform based on CRISPR/Cas12a system and hybridization chain reaction (HCR) circuit was developed. The platform converts miRNA into activators through the HCR circuit, enabling sensitive detection of different miRNAs. The platform demonstrated consistency with RT-qPCR results and potential for clinical diagnosis of cancers and therapy monitoring.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Keyi Long, Gaihua Cao, Yue Qiu, Nannan Yang, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: In this study, we developed an HCR-Cas12a controller for sensitive detection of miR-21. The method exhibited a wide detection range and a low limit of detection. It also eliminates the need for expensive instruments, making it suitable for low-resource settings.
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Agriculture, Multidisciplinary
Yayun Jiang, Chaochuan Zheng, Ming Jin, Ruolan Zhou, Qiaoli Wu, Fuyuan Huang, Yongliang Lou, Laibao Zheng
Summary: We developed a novel CRISPR/Cas12a mediated strand displacement/hybridization chain reaction (CSDHCR) nucleic acid assay for colorimetric detection of foodborne pathogens. The CSDHCR biosensor demonstrated satisfactory linear detection and high sensitivity for nucleic acid targets in the range of 10 fM to 1 nM, with a limit of detection of 4.54 fM. The method also showed promising specificity and sensitivity for the detection of Vibrio vulnificus, a foodborne pathogen, making it a potential alternative for ultrasensitive and visual detection of nucleic acids and foodborne pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Wang, Yaqi Liu, Jixian Yan, Yunqing Liu, Chaomin Gao, Shenguang Ge, Jinghua Yu
Summary: This study proposed a new ultrasensitive miRNA detection method based on CRISPR/Cas12a, utilizing multiple mechanisms such as a 3D DNA walker and 3D-rGO/AuPd nanoparticles to achieve high sensitivity and specificity in detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Wanhe Wang, Jingqi Liu, Xiaoxia Li, Chuankai Lin, Xueliang Wang, Jianhua Liu, Liansheng Ling, Jing Wang
Summary: CRISPR/Cas12a-based biosensors have been widely studied for nucleic acid detection, but this study demonstrates their use in colorimetric detection of serum PSA using an enzyme-free HCR-powered system. The assay shows sensitive and selective detection capabilities in both visual and quantitative modes, highlighting its potential for protein cancer biomarker detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Multidisciplinary
Qiqi Cai, Hanxing Shi, Mengni Sun, Na Ma, Rui Wang, Wenge Yang, Zhaohui Qiao
Summary: A sensitive detection method for Salmonella was developed by coupling immunomagnetic separation with the CRISPR-Cas12a system and the tetrahedral DNA nanostructure-mediated hyperbranched hybridization chain reaction (TDN-hHCR). Salmonella as low as 8 CFU/mL could be easily detected, and it showed potential application in real samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiong Liu, He Zhang, Sainan Gao, Wenjie Ma, Xin Fu, Mei Yang
Summary: A novel colorimetric sensor combining CRISPR/Cas12a with hyperbranched hybridization chain reaction (HB-HCR) was developed for highly sensitive and specific diagnosis of Mycobacterium bovis. The sensor showed a very low limit of detection for Mycobacterium bovis target and demonstrated good stability and accuracy in real biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Min Zhong, Jinbo Liu, Jie Wu, Jinqian Li, Nini Luo, Chuanlong Zhu, Rui Liu, Qianfeng Xia, Huangxian Ju
Summary: This study developed a dual signal amplification strategy for sensitive fluorescent detection of DENV RNA. The strategy utilized a target triggered hybridization chain reaction (HCR) and multiplex proximity ligation activated CRISPR-Cas12a to achieve dual signal amplification. The proposed assay demonstrated high sensitivity and accuracy, making it promising for clinical disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Feng Qiu, Xiaoyan Gan, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a sensitive detection method for nuclear factor-kappa B p50 (NF-KB p50) was proposed by integrating hybridization chain reaction (HCR)-activated Cas12a enzyme with bio-responsive DNA hydrogels. The method achieved highly enhanced electrochemical signals for detecting target molecules at a low detection limit and successfully detected NF-KB p50 in lysate of HeLa cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xuelan Wu, Ting Ju, Zeyang Li, Jingwen Li, Xingwei Zhai, Kun Han
Summary: This study designed and optimized a target-independent fluorescent signal system, Hybridization chain reaction Fluorescence resonance energy transfer (HCR-FRET) system. Combined with CRISPR/Cas12a system, a fluorescent biosensing protocol was developed for the detection of T790 M. The HCR-FRET system showed a detection range from 1 pM to 400 pM with a detection limit of 316 fM. The target-independent property of the HCR-FRET system indicates its potential for parallel detection of other DNA targets.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Rujian Zhao, Chunxu Yu, Baiyang Lu, Bingling Li
Summary: We have reported a universal and signal-on detection platform by coupling the CRISPR-Cas12a system with HCR. By changing the crRNA, different targets can be detected using the CRISPR-HCR pathway. The CRISPR-HCR platform, when coupled with an upstream amplifier, achieves a practical sensitivity as low as similar to aM of ASFV gene in serum.
Article
Biochemistry & Molecular Biology
Nidhi Nandu, Christopher W. Smith, Mahera J. Kachwala, Mehmet Yigit
Summary: Research has shown that two dimensional nanoparticles possess nanozyme activity and can be identified and distinguished using statistical analysis tools for different metal cations' interference effects. Experimental results demonstrate that Ti2+, Co2+, Cu2+, Ni2+, Ca2+, Zn2+, and Mn2+ have minimal impact on nanozyme activity, while Fe2+, Ag+, Hg2+, and Pb2+ ions significantly interfere with the nanozyme reaction.
Article
Biochemical Research Methods
Christopher W. Smith, Mahera J. Kachwala, Nidhi Nandu, Mehmet Yigit
Summary: Researchers have discovered a new type of fluorescent reporter based on a dsDNA template, which can increase the flexibility and efficiency of CRISPR-Cas12a in genetic diagnostics.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alexandre Bernier, Tanner Tobias, Hoang Nguyen, Shreshth Kumar, Beza Tuga, Yusha Imtiaz, Christopher W. Smith, Rajesh Sunasee, Karina Ckless
Summary: The study investigated the impact of cationic cellulose nanocrystals on human blood and endothelial cells, finding that these nanoparticles did not cause significant adverse effects at certain concentrations, which may be beneficial for potential biomedical applications.
Article
Chemistry, Analytical
Christopher W. Smith, Mustafa Salih Hizir, Nidhi Nandu, Mehmet Yigit
Summary: This study presents a biomarker-free detection method for various biological targets using machine learning algorithms and automated selection processes. By using cost-efficient 2D nanoparticles and fluorescently labeled DNA components, nanosensors can be selected and predicted with 100% accuracy, enabling scaled-up data collection for machine learning modeling. This approach minimizes manpower, material cost, computational resources, instrumentation usage, and time, while providing greater predictive accuracy for optical nanosensor arrays.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Mahera J. Kachwala, Christopher W. Smith, Nidhi Nandu, Mehmet Yigit
Summary: A new detection assay called RACECAR has been developed, which can directly detect hepatitis B virus genomic targets in serum samples without an extraction step. The assay is highly specific and sensitive, with a limit of detection of 3 copies of HBV genome.
Article
Food Science & Technology
Christopher W. Smith, Mahera J. Kachwala, Rachel L. Cole, Mehmet Yigit
Summary: A biomarker-free detection assay using an optical nanosensor array was developed to enhance the food safety of citrus juices. By combining machine learning with fluorescence data analysis, the study achieved over 90% accuracy in predicting adulteration of various citrus juices.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)
