Article
Nanoscience & Nanotechnology
Alexander L. Danesi, Dimitra Athanasiadou, Abdulmateen O. Aderinto, Prakash Rasie, Leo Y. T. Chou, Karina M. M. Carneiro
Summary: The study demonstrates that DNA nanostructures can promote site-specific calcium phosphate formation under mineralizing conditions, resulting in amorphous calcium phosphate or hydroxyapatite. This finding sheds light on the mechanisms of crystal nucleation and growth in mineralized tissues, providing insights for mineralized tissue regeneration strategies.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Anastasia Yu. Teterina, Igor V. Smirnov, Irina S. Fadeeva, Roman S. Fadeev, Polina V. Smirnova, Vladislav V. Minaychev, Margarita I. Kobyakova, Aleksandr Yu. Fedotov, Sergey M. Barinov, Vladimir S. Komlev
Summary: The study presented the synthesis of low-temperature calcium phosphate compounds with strontium substitution to improve bioactive properties, indicating the effectiveness of using strontium in OCP for enhancing biocompatibility in composite materials intended for bone repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ahmed Salama
Summary: Researchers are increasingly focusing on the development of unique materials from sustainable and renewable resources, with chitosan and its derivatives being versatile candidates for preparing attractive materials. Various preparation strategies for chitosan/calcium phosphate composites have been discussed and are widely applied in the biomedical and environmental fields.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Lian-Hua Fu, Yilin Wan, Chunying Li, Chao Qi, Ting He, Chen Yang, Yifan Zhang, Jing Lin, Peng Huang
Summary: This study presents a novel nanoplatform GMCD for tumor microenvironment-specific activatable cascade catalytic reactions-augmented PDT, which can effectively accumulate at tumor sites, promote GOx catalytic reaction, alleviate tumor hypoxia, enhance production of cytotoxic singlet oxygen, and amplify oxidative damage of cancer cells. GMCD demonstrates superior therapeutic effects on 4T1-tumor bearing mice through a long-term cascade catalytic reactions-augmented PDT.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Joschka Hellmeier, Rene Platzer, Vanessa Muehlgrabner, Magdalena C. Schneider, Elke Kurz, Gerhard J. Schuetz, Johannes B. Huppa, Eva Sevcsik
Summary: DNA origami structures provide flexible scaffolds for organizing biomolecules with nanometer precision, but functionalizing them with proteins while maintaining stoichiometry and protein functionality remains challenging. This study systematically evaluated different strategies for site-specific decoration of DNA origami structures, with a focus on efficiency, stoichiometry, and protein functionality. The study found that using charge-neutral peptide nucleic acid emerged as the best strategy for achieving high yield and preserving protein functionality.
Article
Engineering, Biomedical
Lotem Gotnayer, Dina Aranovich, Merav Fraenkel, Uri Yoel, Netta Vidavsky
Summary: Thyroid nodules are common findings in neck ultrasonography, but only a small percentage of them are malignant. Fine needle aspiration for cytology is indicated when ultrasound characteristics suggest TN malignancy. However, indeterminate results are common, requiring further evaluation through repeated FNAC or expensive molecular testing. This study suggests that the presence of zinc in microscopic calcifications may serve as a biomarker for TN malignancy.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Michael Erkelenz, Richard Kosinski, Oliver Sritharan, Helene Giesler, Barbara Sacca, Sebastian Schlucker
Summary: In this study, bare gold nanocubes and nanospheres of different sizes were incorporated into a rationally designed 3D DNA origami box. The encapsulated particles exhibit a gold surface that can be functionalized for specific applications in molecular plasmonics such as SERS or SEF.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Susrut Akkineni, Gregory S. Doerk, Chenyang Shi, Biao Jin, Shuai Zhang, Stefan Habelitz, James J. De Yoreo
Summary: The study demonstrates how the supramolecular structures of matrix proteins can be synthetically directed into predetermined patterns while retaining their functionality. They used block copolymer lamellar patterns to direct the assembly of peptide nanoribbons, which template calcium phosphate nucleation by creating a low-energy interface. The results show that the patterned nanoribbons maintain their structure and function, and successfully direct the formation of calcium phosphate with high fidelity.
Article
Chemistry, Multidisciplinary
Tianqing Zhang, Bryan Wei
Summary: Sequence complementarity is crucial in designing specific recognition among DNA nanostructure units through sticky-end cohesion. Binding orthogonality is usually achieved through sticky-end pairs with different sequences. This study explores orthogonal recognition of synthetic DNA constructs solely based on configurational match, by restricting the diversity of sticky-end sequences to a fixed C-G pair. Comprehensive investigations of 2D tessellation and 3D crystallization demonstrate the ability of this new configuration-specific sticky-end cohesion to program specific and precise molecular recognition for high-order DNA self-assembly.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Jihye Lee, Adama Konate, Clement Guibert, Jessem Landoulsi
Summary: In this study, a biomimetic method for growing a hybrid calcium phosphate (CaP) - collagen coating is described. The versatility of layer-by-layer (LbL) method is explored to incorporate collagen and alkaline phosphatase (ALP), and control their organization and bioactivity. The presence of collagen fibrils in the LbL film plays a crucial role in the growth mechanism of the biomimetic mineral layer. The approach provides a straightforward way for designing biomimetic hybrid coatings in biomedical applications.
SURFACES AND INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Elmar Weinhold, Banani Chakraborty
Summary: The recent focus of DNA nanotechnology has been on functionalizing DNA origami for multiple applications. A novel DNA enzyme nano-factory has been constructed which significantly enhances local concentration and can potentially be expanded to a multi-enzyme system controlled by simple to complex molecules made of DNA, RNA or proteins.
Review
Biochemistry & Molecular Biology
Clement Guibert, Jessem Landoulsi
Summary: Biomineralization is the process by which organisms produce hard inorganic matter from soft tissues, with sophisticated toolkit and biological system regulation in place. Two main views have emerged regarding the mechanism of CaP biomineralization, with one focusing on in vivo systems and the other on physicochemistry research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Yuchen Zhang, Shaoyang Ma, Jiaming Nie, Zhongbo Liu, Faming Chen, Ang Li, Dandan Pei
Summary: Bone mineralization is a complex process involving the interplay between organic and inorganic components of bone tissues. This review focuses on the evolution of mineral precursors in the bone mineralization process and highlights the tight control organisms have over each step. The connection between biomineralization and biomaterial design strategies is discussed, along with the detailed implementation of mineral precursor-based biomaterials in various bone graft materials.
Article
Polymer Science
Sunny Skaria, Kenneth J. Berk
Summary: This study aimed to synthesize and characterize a novel methacrylate-functionalized calcium phosphate for use in innovative dental composites. The results showed that adding MCP improved the flexural strength and translucency of the composites, as well as promoted the surface precipitation of hydroxyapatite.
Article
Chemistry, Multidisciplinary
Min-juan Shen, Chen-yu Wang, Dong-xiao Hao, Jia-xin Hao, Yi-fei Zhu, Xiao-xiao Han, Lige Tonggu, Ji-hua Chen, Kai Jiao, Franklin R. Tay, Li-na Niu
Summary: The idea that RNA can play nonbiological roles in the nanomaterial world is revolutionary. RNA molecules can self-assemble into various nanostructures and have the potential to replicate bone healing. The development of RNA-biomineral nanomachines can mimic bone formation and create an environment conducive to rapid biomineralization and osteogenesis.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Dandan Li, Jiang Li, Jiao Hu, Mingjie Tang, Peng Xiu, Yunchang Guo, Tunan Chen, Ning Mu, Lihua Wang, Xuehua Zhang, Guizhao Liang, Huabin Wang, Chunhai Fan
Summary: Understanding how A beta 42 oligomers affect neurons from a mechanobiological perspective is crucial for studying neurodegenerative diseases. Using atomic force microscopy (AFM), we quantitatively investigate the nanomechanical properties of neurons exposed to A beta 42 oligomers. We establish a method called heterogeneity-load-unload nano mechanics (HLUN) which allows us to comprehensively profile the mechanical properties of living neurons and correlate them with biological effects.
Article
Chemistry, Multidisciplinary
Lulu Huang, Xiuhai Mao, Jie Li, Qian Li, Jianlei Shen, Mengmeng Liu, Chunhai Fan, Yang Tian
Summary: By designing dual-emissive gold nanoparticles with varying spikiness, the interactions between spiky nanoparticles and cells were investigated. It was found that the nanospikes regulated myosin IIA recruitment at the cell membrane, enhancing cellular uptake efficiency. Moreover, the spiky nanoparticles facilitated endocytosis dynamics. These findings provide insights into the intracellular fate of nanoparticles and the development of theranostic nanocarriers.
Article
Chemistry, Multidisciplinary
Daoxia Guo, Xiaoyuan Ji, Hui Xie, Jia Ma, Chunchen Xu, Yanfeng Zhou, Nan Chen, Hui Wang, Chunhai Fan, Haiyun Song
Summary: Cancer-associated fibroblasts (CAFs) play a role in chemoresistance and immunosuppression, but direct depletion may lead to increased invasiveness and metastasis. Gemini-like targeting nanoparticles (NPs) have been designed to transform CAFs and eliminate cancer cells, enhancing drug susceptibility and immune cell infiltration. In mouse models of various resistant cancers, a single dose of these NPs resulted in improved chemosensitivity, immune activation, tumor regression, and long-term protection against tumor rechallenge.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Chunchen Xu, Xiaoyuan Ji, Yanfeng Zhou, Yuchun Cheng, Daoxia Guo, Qian Li, Nan Chen, Chunhai Fan, Haiyun Song
Summary: A study introduces a novel approach to revitalize tumor-associated dendritic cells (TADCs) and enhance their anti-tumor response. This is achieved by using multilevel lipid rewiring nanoparticles to promote TADC maturation and restore their inflammatory cytokine production, cytotoxic T cell recruitment, and tumor inhibition.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Shuangshuang Wang, Huaibin Yu, Longjie Li, Meizhou Zhang, Yu Fu, Zi'an Lin, Jinsheng Li, Fangrui Zhong, Hongmei Liu, Yuzhou Wu
Summary: We report the development of inhibitory and noninhibitory fluorescent turn-on probes for specific labeling of GPx4 in live cells. These probes allow visualization of GPx4 expression levels and degradation kinetics, and reveal the real-time response of GPx4 to cellular selenium availability. They also have potential applications in predicting the sensitivity of GPx4-related ferroptosis drugs.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Haipei Zhao, Xuehao Xiu, Mingqiang Li, Shaobo Dai, Mingyang Gou, Leyang Tao, Xiaolei Zuo, Chunhai Fan, Zhongqun Tian, Ping Song
Summary: Dynamic interactions of enzymes are important in regulating cellular metabolism. A super DNA-enzymes molecule was constructed for controlling and detecting metabolism. The programmable SDEM shows improved detection limit and reaction rate compared to free enzymes. The SDEM can be assembled and disassembled using a hairpin structure and a displacement DNA strand for multiple cycles. An entropically driven catalytic assembly enables quick switching between different SDEMs for detecting different metabolisms on demand.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
News Item
Chemistry, Multidisciplinary
Ping Song, Chunhai Fan
Summary: A new high-dimensional microfluidic approach allows for the quantitative isolation of aptamers with customizable binding affinities, presenting a challenging yet promising method for constructing aptamers with desired target-binding affinities.
Article
Nanoscience & Nanotechnology
Fangfei Yin, Haipei Zhao, Shasha Lu, Juwen Shen, Min Li, Xiuhai Mao, Fan Li, Jiye Shi, Jiang Li, Baijun Dong, Wei Xue, Xiaolei Zuo, Xiurong Yang, Chunhai Fan
Summary: The article introduces a DNA-encoded molecular classifier that can classify multidimensional molecular clinical data and has been tested in prostate cancer patients.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Guang-Jing Feng, Yang-Fan Guo, Yuming Tang, Min Li, Yufei Jia, Zhimeng Li, Shuangshuang Wang, Hongmei Liu, Yuzhou Wu, Hai Dong
Summary: The potential of thioglucoside analogues as antidiabetic drugs was investigated in this study. The analogues showed good stability, low toxicity, and high inhibitory activity, making them promising candidates for new gliflozin drugs. Additionally, the synthesis of these analogues is simple, efficient, and cost-effective.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Kun Dai, Yang Xu, Yang Yang, Jianfeng Shen, Xiaoguo Liu, Xinyi Tu, Lu Yu, Xiaodong Qi, Jiang Li, Lihua Wang, Xiaolei Zuo, Yingbin Liu, Hao Yan, Chunhai Fan, Guangbao Yao
Summary: This study utilizes single-stranded RNA (ssRNA) origami as ligands for nucleic acid-sensing receptors, achieving improved stability, targeting, and immunogenicity prediction. The ssRNA self-folds into compact nanoparticles with defined shapes and exhibits resistance against degradation in cells. It can activate macrophages and neutrophils in the tumor microenvironment, leading to anti-tumoral immune response and tumor growth retardation in a mouse model. This ssRNA origami strategy provides a new solution for ligand design and biomedical applications of nucleic acid sensors.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Kun Dai, Chen Gong, Yang Xu, Fei Ding, Xiaodong Qi, Xinyi Tu, Lu Yu, Xiaoguo Liu, Jiang Li, Chunhai Fan, Hao Yan, Guangbao Yao
Summary: This study presents a transcription-based approach to quantitatively integrate nucleoside analogue molecules into RNA nanostructures. The integrated RNA origami not only maintains its morphology and biostability, but also gains new biomedical functions, such as innate immune recognition after integrating epigenetic nucleoside analogues, and synergistic effects on tumor cell killing after integrating therapeutic nucleoside analogues. This research provides a promising method for the quantitative integration of functional nucleoside analogues into RNA nanostructures, offering valuable insights for the development of multifunctional RNA origamis.
Article
Multidisciplinary Sciences
Hui Lv, Nuli Xie, Mingqiang Li, Mingkai Dong, Chenyun Sun, Qian Zhang, Lei Zhao, Jiang Li, Xiaolei Zuo, Haibo Chen, Fei Wang, Chunhai Fan
Summary: We demonstrate a general-purpose DNA integrated circuit (DIC) system by integrating multilayer DNA-based programmable gate arrays (DPGAs), which can be reconfigured and programmed to implement a wide range of circuits. We also show that DNA origami registers can control the asynchronous execution of cascaded DPGAs, and the integration of a DPGA with an analog-to-digital converter can be used for disease classification.
Article
Chemistry, Multidisciplinary
Qinqin Hu, Zongxuan Tong, Ayimukedisi Yalikong, Li-Ping Ge, Qiang Shi, Xinyu Du, Pu Wang, Xi-Yu Liu, Wuqiang Zhan, Xia Gao, Di Sun, Tong Fu, Dan Ye, Chunhai Fan, Jie Liu, Yun-Shi Zhong, Yi-Zhou Jiang, Hongzhou Gu
Summary: Biomarker discovery is crucial for understanding and diagnosing malignant diseases, but the lack of sensitive methods to identify rare disease-specific molecules remains a challenge. This study presents MORAC, a method that utilizes DNAzymes to identify candidate biomarkers with low abundance. By exploiting the catalysis and affinity of DNAzymes, previously unknown biomarkers with clear clinical value can be identified.
Review
Chemistry, Multidisciplinary
Longjiang Ding, Xiaoliang Chen, Wenhe Ma, Jiang Li, Xiaoguo Liu, Chunhai Fan, Guangbao Yao
Summary: How far we can push chemical self-assembly is one of the most important scientific questions of the century. Colloidal self-assembly is a bottom-up technique for the rational design of functional materials with desirable collective properties. The regioselective control over the formation of DNA bonds on the particle surface has enabled unprecedented complexity in colloidal self-assembly, thanks to the advances in surface chemistry and structural DNA nanotechnology. This review summarizes the recent advances in DNA-mediated regioselective surface encoding of colloids, with a focus on its introduction and its crucial role in the self-assembly of colloidal structures.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Chemistry, Physical
Wenjing Jiang, Jingrou Li, Zi'an Lin, Jing Guo, Jiaxin Ma, Zhen Wang, Meizhou Zhang, Yuzhou Wu
Summary: In recent years, the development of DNA self-assembly technology has fueled the advancement of DNA origami technology. This technology enables the construction of DNA nanostructures with different shapes, which find wide applications in nanomaterial preparation and drug delivery. These structures possess accurate addressability, excellent programmability, and good biocompatibility, providing a highly precise platform for nanomaterial fabrication.