Activity profiling and crystal structures of inhibitor-bound SARS-CoV-2 papain-like protease: A framework for anti–COVID-19 drug design

Profiling of flaviviral NS2B-NS3 protease specificity provides a structural basis for the development of selective chemical tools that differentiate Dengue from Zika and West Nile viruses

(2020) Wioletta Rut et al.   ANTIVIRAL RESEARCH

Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China

(2020) Chaolin Huang et al.   LANCET

A novel coronavirus outbreak of global health concern

(2020) Chen Wang et al.   LANCET

Characterization and noncovalent inhibition of the deubiquitinase and deISGylase activity of SARS-CoV-2 papain-like protease

(2020) Brendan T Freitas et al.   ACS Infectious Diseases

Disulfiram can inhibit MERS and SARS coronavirus papain-like proteases via different modes

(2018) Min-Han Lin et al.   ANTIVIRAL RESEARCH

Selective Substrates and Activity-Based Probes for Imaging of the Human Constitutive 20S Proteasome in Cells and Blood Samples

(2018) Wioletta Rut et al.   JOURNAL OF MEDICINAL CHEMISTRY

Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes

(2018) David S. Hewings et al.   Nature Communications

Emerging challenges in the design of selective substrates, inhibitors and activity-based probes for indistinguishable proteases

(2017) Paulina Kasperkiewicz et al.   FEBS Journal

Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity

(2017) Marcin Poreba et al.   Nature Protocols

Structural basis of the specificity of USP18 toward ISG15

(2017) Anja Basters et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of the specificity of USP18 toward ISG15

(2017) Anja Basters et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease

(2016) Miklós Békés et al.   MOLECULAR CELL

SARS and MERS: recent insights into emerging coronaviruses

(2016) Emmie de Wit et al.   NATURE REVIEWS MICROBIOLOGY

The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds

(2015) Yahira M. Báez-Santos et al.   ANTIVIRAL RESEARCH

SARS hCoV papain-like protease is a unique Lys48linkage-specific di-distributive deubiquitinating enzyme

(2015) Miklós Békés et al.   BIOCHEMICAL JOURNAL

A Native Chemical Ligation Handle that Enables the Synthesis of Advanced Activity-Based Probes: Diubiquitin as a Case Study

(2014) Monique P. C. Mulder et al.   CHEMBIOCHEM

From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design

(2014) Rolf Hilgenfeld   FEBS Journal

Structural Basis for the Ubiquitin-Linkage Specificity and deISGylating Activity of SARS-CoV Papain-Like Protease

(2014) Kiira Ratia et al.   PLoS Pathogens

The Ubiquitin-Conjugating System: Multiple Roles in Viral Replication and Infection

(2014) Arianna Calistri et al.   Cells

On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases

(2013) Reggy Ekkebus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

MERS-CoV papain-like protease has deISGylating and deubiquitinating activities

(2013) Anna M. Mielech et al.   VIROLOGY

Ubiquitin-Based Probes Prepared by Total Synthesis To Profile the Activity of Deubiquitinating Enzymes

(2012) Annemieke de Jong et al.   CHEMBIOCHEM

Activity, Specificity, and Probe Design for the Smallpox Virus Protease K7L

(2012) Alexander E. Aleshin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia

(2012) Ali M. Zaki et al.   NEW ENGLAND JOURNAL OF MEDICINE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin

(2010) Farid El Oualid et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Deubiquitinating and Interferon Antagonism Activities of Coronavirus Papain-Like Proteases

(2010) M. A. Clementz et al.   JOURNAL OF VIROLOGY

Emerging principles in protease-based drug discovery

(2010) Marcin Drag et al.   NATURE REVIEWS DRUG DISCOVERY

Severe Acute Respiratory Syndrome Coronavirus Papain-Like Protease Ubiquitin-Like Domain and Catalytic Domain Regulate Antagonism of IRF3 and NF- B Signaling

(2009) M. Frieman et al.   JOURNAL OF VIROLOGY

Positional-scanning fluorigenic substrate libraries reveal unexpected specificity determinants of DUBs (deubiquitinating enzymes)

(2008) Marcin Drag et al.   BIOCHEMICAL JOURNAL

A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication

(2008) K. Ratia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA