Article
Chemistry, Multidisciplinary
Xian Xie, Zhuo Li, Xuefeng Yang, Boguang Yang, Zhixian Zong, Xuemei Wang, Liting Duan, Sien Lin, Gang Li, Liming Bian
Summary: In this study, a peptide fiber-reinforced hydrogel was developed, where the stiff β-sheet peptide fiber acted as a multivalent cross-linker to enhance the macroscopic stability of the hydrogel. The dynamic imine cross-link between the peptide fiber and polymer network endowed the hydrogel with a microscopically dynamic network. The resulting fibrillar nanocomposite hydrogel, with its cell-adaptable dynamic network, significantly enhanced cell-matrix and cell-cell interactions, promoting mechanotransduction, metabolic energetics, and osteogenesis of encapsulated stem cells. Additionally, the hydrogel could deliver a fiber-attached inductive drug to further enhance bone regeneration. This work provides valuable guidance for the design of cell-adaptive and bioactive biomaterials for therapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Katarina D. Kovacevic, Stefan Greisenegger, Agnes Langer, Georg Gelbenegger, Nina Buchtele, Ingrid Pabinger, Karin Petroczi, Shuhao Zhu, James C. Gilbert, Bernd Jilma
Summary: BT200, a third generation anti-VWF aptamer, showed potential for secondary stroke prevention in patients with large artery atherosclerosis stroke by reducing VWF activity, VWF-dependent platelet function and prolonging closure times. Its target concentration-dependent manner and favorable safety profile in phase I trials make it an interesting drug candidate.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Ruihong Li, Juan Liu, Jie Ma, Xuer Sun, Yi Wang, Jiexin Yan, Qunfang Yu, Jinmei Diao, Chun Yang, Lola M. Reid, Yunfang Wang
Summary: The liver functions depend on interactions between parenchymal cells and the microenvironment, with biological macromolecules in the matrix playing a key role. Rat liver biomatrix scaffold prepared from adult rats is more effective in enhancing hepatic spheroid function. Fibronectin and fibrinogen show potential for improving hepatocyte function, promoting cell aggregation and activating Wnt/beta-catenin pathway.
Article
Hematology
Nikolett Wohner, Silvie Sebastian, Vincent Muczynski, Dana Huskens, Bas de Laat, Philip G. de Groot, Peter J. Lenting
Summary: The study reveals that VWF strings secreted from endothelial cells are only partially covered with platelets, and osteoprotegerin plays a role in regulating platelet adhesion to VWF. This regulatory mechanism may be more relevant under inflammatory conditions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Chiara Motta, Angelica Pellegrini, Stefano Camaione, Joan Geoghegan, Pietro Speziale, Giulia Barbieri, Giampiero Pietrocola
Summary: In this study, the interaction between the secreted von Willebrand factor-binding protein (vWbp) from Staphylococcus aureus and coagulation factors prothrombin and fibrinogen (Fbg) was investigated. It was found that vWbp-activated Factor XIII (FXIII) catalyzes the incorporation of amino-donor dansylcadaverine into region A of fibronectin-binding protein A (FnBPA). The study suggests a novel mechanism by which transglutaminase action of FXIII and TG2 contributes to the entrapment and persistence of S. aureus in blood and host tissues.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Noel G. Tsyu, Aleksey V. Belyaev
Summary: A multi-subunit glycoprotein called Von Willebrand factor (VWF) in blood plasma is responsible for mediating platelet adhesion to damaged blood vessel walls. The adhesion of VWF to collagen is crucial for platelet hemostasis and thrombosis, acting as a molecular bridge between injury and platelet adhesion receptors. This study proposes a simulation framework for VWF-mediated platelet adhesion under the influence of shear flow, using particles representing VWF multimers and platelets immersed in a viscous fluid. The model considers the flattened platelet shape and balances detail of description with computational complexity.
INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING
(2023)
Article
Hematology
Maurice Swinkels, Sophie Hordijk, Petra E. Buergisser, Johan A. Slotman, Tom Carter, Frank W. G. Leebeek, A. J. Gerard Jansen, Jan Voorberg, Ruben Bierings
Summary: This study investigated the release of VWF and VWFpp from individual alpha-granules in response to different physiological stimuli. The results showed that the secretion of alpha-granule cargo increased in a dose-dependent manner upon activation of PAR-1 and GPVI signaling pathways. However, there were differential release patterns for VWF and VWFpp.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Sergey Okhota, Sergey Kozlov, Yuliya Avtaeva, Ivan Melnikov, Olga Saburova, Konstantin Guria, Evgeny Matroze, Zufar Gabbasov
Summary: This study examined platelet adhesion mediated by von Willebrand factor (VWF) at high shear rates in patients with premature coronary artery disease (CAD). The researchers found that patients with premature CAD exhibited a greater GPIb-mediated component of platelet adhesion compared to control patients. This suggests a potential role of excessive VWF-platelet interactions in the development of premature CAD.
Article
Hematology
Catarina Monteiro, Ana Goncalves, Monica Pereira, Catarina Lau, Sara Morais, Rosario Santos
Summary: Platelet-type von Willebrand disease (PT-VWD) is a rare autosomal dominant bleeding disorder characterized by increased platelet aggregation and enhanced affinity of GPIba to VWF. A novel heterozygous variant of GP1BA, likely causing the PT-VWD phenotype, was identified in the patient through genetic sequencing.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Cell Biology
Thorsten Kragh, Isabell Pekrul, Helmut W. Ott, Michael Spannagl, Patrick Moehnle
Summary: The interaction of platelets with von Willebrand factor is crucial for primary hemostasis. The study proposes a method for laboratory assessment and reporting of platelet von Willebrand factor, providing estimates of activity and concentration of platelet von Willebrand factor in healthy individuals.
Article
Hematology
Daniel A. Keesler, Tricia L. Slobodianuk, Caroline E. Kochelek, Chad W. Skaer, Sandra L. Haberichter, Veronica H. Flood
Summary: The study found that fibronectin can bind to VWF with a strong affinity similar to that of collagen IV. Under specific conditions, VWF serves as a common factor linking platelet adhesion to various ECM proteins and facilitating hemostasis.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Johana Hrdinova, Delia I. Fernandez, Bogac Ercig, Bibian M. E. Tullemans, Dennis P. L. Suylen, Stijn M. Agten, Kerstin Jurk, Tilman M. Hackeng, Karen Vanhoorelbeke, Jan Voorberg, Chris P. M. Reutelingsperger, Kanin Wichapong, Johan W. M. Heemskerk, Gerry A. F. Nicolaes
Summary: This study designed and synthesized stable cyclic peptides to interfere with the interaction between VWF A1 domain and GPIb alpha. The selected peptides showed low binding free energy and retained their interference in the binding of VWF to GPIb-V-IX, as confirmed by flow cytometry. These peptides phenotypically mimicked the changes seen with the anti-VWF A1 domain antibody CLB-RAg35, although they were less potent. The improved peptide opt-mono-ORbIT demonstrated increased inhibitory activity under flow.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Rameesa D. Syed Mohammed, Francis D. O. Ablan, Nicholas M. McCann, Mohammed M. Hindi, Muriel C. Maurer
Summary: Factor XIII (FXIII) catalyzes the formation of crosslinks between reactive glutamines (Q) and lysines (K). FXIII can be activated proteolytically (FXIII-A*) or nonproteolytically (FXIII-A degrees). The activity and specificity of FXIII-A* and FXIII-A degrees towards different substrates were investigated to gain a better understanding of how FXIII-A targets different substrates.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Justine H. Ryu, Kenneth A. Bauer, Sol Schulman
Summary: This report describes the successful perioperative management of a patient with type 2N VWD using the recombinant FVIII product efanesoctocog alfa. By decoupling the FVIII-VWF interaction, efanesoctocog alfa achieves prolonged FVIII circulation and mitigates potential cardiovascular risks.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Critical Care Medicine
Xue D. Manz, Robert Szulcek, Xiaoke Pan, Petr Symersky, Chris Dickhoff, Jisca Majolee, Veerle Kremer, Elisabetta Michielon, Ekaterina S. Jordanova, Teodora Radonic, Irene Bijnsdorp, Sander R. Piersma, Thang Pham, Connie R. Jimenez, Anton Vonk Noordegraaf, Frances S. de Man, Reinier A. Boon, Jan Voorberg, Peter L. Hordijk, Jurjan Aman, Harm Jan Bogaard
Summary: This study identifies the role of von Willebrand factor (vWF) in chronic thromboembolic pulmonary hypertension (CTEPH). Increased vWF levels in plasma and pulmonary endothelium of CTEPH patients contribute to elevated platelet adhesion to CTEPH endothelium. The increased expression of nuclear factor (NF)-kappa B2 and epigenetic modifications of the vWF promoter play a critical role in driving vWF transcription and promoting a prothrombotic phenotype in CTEPH-pulmonary artery endothelial cells.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Hematology
Li Guo, Rick Kapur, Rukshana Aslam, Edwin R. Speck, Anne Zufferey, Yajing Zhao, Michael Kim, Alan H. Lazarus, Heyu Ni, John W. Semple
Review
Biochemistry & Molecular Biology
Yiming Wang, Heyu Ni
CELLULAR AND MOLECULAR LIFE SCIENCES
(2016)
Review
Medical Laboratory Technology
Xiaohong Ruby Xu, Dan Zhang, Brigitta Elaine Oswald, Naadiya Carrim, Xiaozhong Wang, Yan Hou, Qing Zhang, Christopher Lavalle, Thomas McKeown, Alexandra H. Marshall, Heyu Ni
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2016)
Article
Endocrinology & Metabolism
Alison Cameron-Vendrig, Adili Reheman, M. Ahsan Siraj, Xiaohong Ruby Xu, Yiming Wang, Xi Lei, Talat Afroze, Eric Shikatani, Omar El-Mounayri, Hossein Noyan, Ralph Weissleder, Heyu Ni, Mansoor Husain
Review
Pediatrics
Darko Zdravic, Issaka Yougbare, Brian Vadasz, Conglei Li, Alexandra H. Marshall, Pingguo Chen, Jens Kjeldsen-Kragh, Heyu Ni
SEMINARS IN FETAL & NEONATAL MEDICINE
(2016)
Article
Immunology
Xavier Clemente-Casares, Siyavash Hosseinzadeh, Iulia Barbu, Sarah A. Dick, Jillian A. Macklin, Yiming Wang, Abdul Momen, Crystal Kantores, Laura Aronoff, Maylis Farno, Tiffany M. Lucas, Joan Avery, Dorrin Zarrin-Khat, Heidi J. Elsaesser, Babak Razani, Kory J. Lavine, Mansoor Husain, David G. Brooks, Clinton S. Robbins, Myron Cybulsky, Slava Epelman
Article
Hematology
Xiaohong Ruby Xu, George M. Yousef, Heyu Ni
Article
Multidisciplinary Sciences
Louise J. Eltringham-Smith, Ruoying Yu, Syed M. Qadri, Yiming Wang, Varsha Bhakta, Edward L. Pryzdial, Jeffrey R. Crosby, Heyu Ni, William P. Sheffield
SCIENTIFIC REPORTS
(2019)
Article
Hematology
Alexander Leatherdale, D'Andra Parker, Subia Tasneem, Yiming Wang, Dominique Bihan, Arkadiusz Bonna, Samir W. Hamaia, Peter L. Gross, Heyu Ni, Bradley W. Doble, David Lillicrap, Richard W. Farndale, Catherine P. M. Hayward
Summary: Multimerin 1 enhances platelet adhesion synergistically with other triple-helical collagen peptides by binding to highly conserved GPAGPOGPX motifs in fibrillar collagens. Moreover, Mmrn1-deficient mice showed impaired platelet adhesion and thrombus formation in the ferric chloride injury model.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Peter A. A. Norris, Gurleen Kaur, Ramsha Khan, Guangheng Zhu, Heyu Ni, Alan H. Lazarus
Summary: Anti-CD44 exhibits anti-inflammatory effects in murine ITP by inhibiting macrophage phagocytosis through blockade of Fc gamma R, potentially serving as an alternative to IVIg. The specific Fc gamma R blockade mechanism of anti-CD44 contributes to its therapeutic efficacy in ITP models, highlighting its potential as a treatment option.
Article
Biochemistry & Molecular Biology
Ming Liu, Gan Wang, Runjia Xu, Chuanbin Shen, Heyu Ni, Ren Lai
Summary: Soy isoflavones genistein and daidzein were found to inhibit thrombosis formation, particularly under high shear force. They interact with 14-3-3ζ and suppress multiple platelet activation pathways and outside-in signaling transduction. These findings suggest that 14-3-3ζ is a novel target for genistein and daidzein in platelet inhibition.
Article
Chemistry, Medicinal
Wenjing Ma, Zackary Rousseau, Sladjana Slavkovic, Chuanbin Shen, George M. M. Yousef, Heyu Ni
Summary: This study found that Doxorubicin induced platelet activation and increased macrophage phagocytosis of platelets, leading to accelerated platelet clearance. Salvianolic acid C (SAC), derived from Danshen root, was found to inhibit Doxorubicin-induced platelet activation more effectively than current anti-platelet drugs. Doxorubicin also enhanced platelet-cancer cell interaction, which was alleviated by SAC.
Article
Hematology
Guangheng Zhu, Qing Zhang, Emily C. Reddy, Naadiya Carrim, Yunfeng Chen, Xiaohong Ruby Xu, Miao Xu, Yiming Wang, Yan Hou, Li Ma, Yan Li, Min Rui, Tania N. Petruzziello-Pellegrini, Christopher Lavalle, Tyler W. Stratton, Xi Lei, Reheman Adili, Pingguo Chen, Cheng Zhu, John A. Wilkins, Richard O. Hynes, John Freedman, Heyu Ni