Article
Biochemistry & Molecular Biology
Valentina Russo, Mohammad El Khatib, Giuseppe Prencipe, Annunziata Mauro, Oriana Di Giacinto, Arlette A. Haidar-Montes, Fanny Pulcini, Beatrice Dufrusine, Adrian Cervero-Varona, Melisa Faydaver, Chiara Di Berardino, Enrico Dainese, Paolo Berardinelli, Matthias Schnabelrauch, Barbara Barboni
Summary: This study fabricated biomimetic scaffolds with aligned fibers, resembling native tendon tissue, and demonstrated their ability to guide the differentiation of amniotic epithelial stem cells (AECs) and enhance their immunomodulatory potential. These findings support the biomimicry of PLGA 3D scaffolds and suggest their potential for promoting tendon regeneration.
Article
Biochemistry & Molecular Biology
Valentina Russo, Annunziata Mauro, Alessia Peserico, Oriana Di Giacinto, Mohammad El Khatib, Maria Rita Citeroni, Emanuela Rossi, Angelo Canciello, Eleonora Mazzotti, Barbara Barboni
Summary: This research demonstrates the potential of amniotic epithelial stem cells (AECs) in promoting tendon regeneration. The study found that AECs can trans-differentiate into cell phenotypes with varying abilities to facilitate regeneration. The findings suggest that AECs with an epithelial phenotype have a greater impact on shifting the inflammatory response to a pro-regenerative response, while AECs with a tendon-like phenotype mainly affect the proliferative phase of regeneration. These results provide support for using AECs as a feasible and effective strategy for the treatment of acute tendinopathies.
Review
Cell & Tissue Engineering
Amirhesam Babajani, Kasra Moeinabadi-Bidgoli, Farnaz Niknejad, Hamidreza Rismanchi, Sepehr Shafiee, Siavash Shariatzadeh, Elham Jamshidi, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: This review highlights the potential of human amniotic epithelial cells (hAECs) and their exosomes as a multi-aspect therapeutic approach in COVID-19 to alleviate complications and consequences of the disease. These cells possess immunomodulatory properties that can aid in viral clearance, regulate immune responses, and promote organ regeneration. Additionally, the application of preconditioned and genetically modified hAECs, as well as their use as a drug delivery system, is discussed.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Shaghayegh Doudi, Mansoureh Barzegar, Elham Angouraj Taghavi, Mohammad Eini, Arian Ehterami, Karen Stokes, Jonathan S. Alexander, Majid Salehi
Summary: The human amniotic membrane (hAM) has wide applications in regenerative medicine and shows advantages in multiple fields. This review summarizes the recent applications of hAM in various domains and discusses the pros and cons of different methods for hAM preparation.
Review
Biotechnology & Applied Microbiology
Jose R. Munoz-Torres, Sidney B. Martinez-Gonzalez, Alan D. Lozano-Lujan, Maria C. Martinez-Vazquez, Perla Velasco-Elizondo, Idalia Garza-Veloz, Margarita L. Martinez-Fierro
Summary: The amniotic membrane (AM) is a functional tissue with properties such as immunomodulation, anti-scarring, anti-microbial, pro or anti-angiogenic effects, and tissue growth promotion. It has been used therapeutically in various surgical specialties to enhance surgical outcomes. This review summarizes the use of AM and its mechanisms of action in different surgical areas, aiming to improve the understanding of its therapeutic targets and emphasize its surgical utility in regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Alexandra Muntiu, Andrea Papait, Federica Vincenzoni, Alberto Vitali, Wanda Lattanzi, Pietro Romele, Anna Cargnoni, Antonietta Silini, Ornella Parolini, Claudia Desiderio
Summary: This study characterized the protein composition of the hAMSCs secretome using the FASP digestion and LC-MS approach. Gene ontology classification and functional protein interaction analysis were performed on the data using bioinformatics tools.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Article
Biotechnology & Applied Microbiology
Ambra Costa, Carolina Balbi, Patrizia Garbati, Maria Elisabetta Federica Palama, Daniele Reverberi, Antonella De Palma, Rossana Rossi, Dario Paladini, Domenico Coviello, Pierangela De Biasio, Davide Ceresa, Paolo Malatesta, Pierluigi Mauri, Rodolfo Quarto, Chiara Gentili, Lucio Barile, Sveva Bollini
Summary: Cardiomyocyte renewal is a clinical need for cardiac regeneration, and stem cell paracrine therapy has been shown to provide cardiac protection and enhance repair. In this study, it was found that extracellular vesicles released by fetal amniotic fluid-derived stem cells could promote cardiomyocyte renewal, possibly through the mediation of factors such as Agrin.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Dennis M. L. W. Kruk, Marissa Wisman, Jacobien A. Noordhoek, Mehmet Nizamoglu, Marnix R. Jonker, Harold G. de Bruin, Karla Arevalo Gomez, Nick H. T. ten Hacken, Simon D. Pouwels, Irene H. Heijink
Summary: The study suggests that lung-derived mesenchymal stromal cells (LMSCs) from COPD patients have a cell-repairing effect on alveolar epithelial cell damage, but exhibit abnormalities in the process of alveolar organoid formation, reflecting impaired regenerative responses of lung cells in COPD patients.
Article
Biochemistry & Molecular Biology
Naiyou Liu, Charles M. Bowen, Mohammadali M. Shoja, Karen Larissa Castro de Pereira, Laxmi Priya Dongur, Antonio Saad, William K. Russell, Thomas Christopher Broderick, Jeffrey H. Fair, William Samuel Fagg
Summary: Myofibroblast activation is a cellular response to physiological or pathological insults. In this study, co-cultured amniotic cell conditioned media (coACCM) was found to activate myofibroblast, while amniotic fluid (AF) represses it. These findings suggest that different biologics can elicit different cellular responses and may have broad utility in the clinic and the lab.
Article
Engineering, Biomedical
Zeming Hu, Yang Luo, Renhao Ni, Yiwei Hu, Fang Yang, Tianyu Du, Yabin Zhu
Summary: This article reviews the potential and characteristics of the human amniotic membrane (hAM) in regenerative medicine applications. Firstly, the biological properties and stemness markers of hAM-derived cells are discussed, highlighting hAM as a promising source of stem cells. Then, current knowledge on the collection, preparation, preservation, and decellularization of hAM is systematically summarized, along with their roles in tissue engineering applications. Finally, the recent advances and modifications of hAM in regenerative medicine are highlighted, emphasizing the need for further research to enhance therapeutic effectiveness.
MATERIALS TODAY BIO
(2023)
Review
Biochemistry & Molecular Biology
Ambra Costa, Rodolfo Quarto, Sveva Bollini
Summary: Studies have shown that small extracellular vesicles (EVs) obtained from human amniotic fluid stem cells (hAFSC) and human amniotic fluid (hAF) have regenerative effects and can be used for tissue recovery and treatment of various diseases. However, the isolation, characterization, and quantification methods of EVs remain challenging for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Carlo Ratto, Ornella Parolini, Angelo Alessandro Marra, Valentina Orticelli, Angelo Parello, Paola Campenni, Veronica De Simone, Diletta Trojan, Francesco Litta
Summary: This study reports the successful implantation of amniotic membrane in a patient with cryptoglandular anal fistula. The procedure was performed safely and the patient experienced a smooth recovery without any complications. Follow-up visits showed no pain and inflammation at the previous fistula opening.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Eva Querdel, Marina Reinsch, Liesa Castro, Deniz Kose, Andrea Bahr, Svenja Reich, Birgit Geertz, Barbel Ulmer, Mirja Schulze, Marc D. Lemoine, Tobias Krause, Marta Lemme, Jascha Sani, Aya Shibamiya, Tim Stuedemann, Maria Koehne, Constantin von Bibra, Nadja Hornaschewitz, Simon Pecha, Yusuf Nejahsie, Ingra Mannhardt, Torsten Christ, Hermann Reichenspurner, Arne Hansen, Nikolai Klymiuk, M. Krane, C. Kupatt, Thomas Eschenhagen, Florian Weinberger
Summary: The transplantation of engineered heart tissue (EHT) patches resulted in partial remuscularization of the injured heart and improved left ventricular function in a dose-dependent manner in guinea pig and pig models. Human-scale patches were successfully transplanted onto pigs in a proof-of-principle study.
Article
Cell & Tissue Engineering
Qiuwan Zhang, Shixia Bu, Junyan Sun, Minhua Xu, Xiaofen Yao, Kunyan He, Dongmei Lai
STEM CELL RESEARCH & THERAPY
(2017)
Article
Multidisciplinary Sciences
Minhua Xu, Junyan Sun, Qian Wang, Qiuwan Zhang, Chunsheng Wei, Dongmei Lai
Article
Cell Biology
Qian Wang, Shixia Bu, Dedong Xin, Boning Li, Lan Wang, Dongmei Lai
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Public, Environmental & Occupational Health
Junyan Sun, Ying Guo, Li Ma, Zhaoxia Qian, Dongmei Lai
Article
Cell & Tissue Engineering
Yating Huang, Zhijie Ma, Xiaojun Kuang, Qiuwan Zhang, Haiyan Li, Dongmei Lai
Summary: The study demonstrates that SA-BG-encapsulated hAECs or CM can treat chemotherapy-induced POF by protecting granulosa cell function and enhancing ovarian vascularization, providing a novel strategy for delivering hAECs for POF treatment.
STEM CELL RESEARCH & THERAPY
(2021)
