Article
Biochemistry & Molecular Biology
Mingda Li, Jie Jia, Shanshan Li, Baocheng Cui, Jiao Huang, Zhaoming Guo, Kun Ma, Li Wang, Changhao Cui
Summary: This study explored the effects of tendon stem cell-derived exosomes (TSC-exo) on tendon stem cells (TSCs), finding that TSC-exo were enriched in transforming growth factor beta (TGF beta) which accelerated the proliferation and migration of TSCs. Additionally, TGF beta carried by TSC-exo activated the TGF beta-Smad2/3 and ERK1/2 signaling pathways in TSCs, ultimately regulating the downstream molecule MMP2.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Hua Shen, Ryan A. Lane
Summary: Achilles tendon rupture, a common sports-related injury, often leads to long-term pain and functional deficits. Previous studies have shown that extracellular vesicles from inflammation-primed adipose-derived stem cells (iEVs) can alleviate the early inflammatory response to tendon injury. This study aimed to investigate the mechanistic roles of iEVs in regulating the injury response of tendons and promoting regeneration.
Article
Cell Biology
Mingzhao Zhang, Hengchen Liu, Manyu Shi, Tingting Zhang, Wenjun Lu, Shulong Yang, Qingbo Cui, Zhaozhu Li
Summary: The study showed that hepatocyte growth factor (HGF)-expressing tendon stem cells (TSCs + HGF) have a significant promoting effect on tendon healing in rats, mainly by reducing inflammation and fibrosis. This provides a novel therapeutic strategy for tendon injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Hengchen Liu, Mingzhao Zhang, Manyu Shi, Tingting Zhang, Wenjun Lu, Shulong Yang, Qingbo Cui, Zhaozhu Li
Summary: The study demonstrated that ADSC-Exos promoted the proliferation, migration, and tenogenic differentiation of TSCs, possibly through the activation of the SMAD signaling pathways. Additionally, ADSC-Exos inhibited early inflammatory reactions and promoted tendon healing in vivo.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Yang An, Shuyan Lin, Xiaojie Tan, Shiou Zhu, Fangfei Nie, Yonghuan Zhen, Luosha Gu, Chunlei Zhang, Baicheng Wang, Wei Wei, Dong Li, Junhao Wu
Summary: ADSCs-EXOs modulate immune responses and inflammation, promote angiogenesis, accelerate proliferation and re-epithelization of skin cells, and regulate collagen remodelling to inhibit scar hyperplasia in wounds. Compared with ADSCs therapeutics, ADSCs-EXOs have high stability, easy storage, immune system non-rejection, homing effect, and easily controlled dosage. In patients with diabetes mellitus, ADSCs-EXOs can improve fat grafting and promote wound healing.
CELL PROLIFERATION
(2021)
Review
Medicine, Research & Experimental
Li Jiang, Jingwei Lu, Yixuan Chen, Kexin Lyu, Longhai Long, Xiaoqiang Wang, Tianzhu Liu, Sen Li
Summary: Tendon injury is a common disorder caused by overuse or trauma. Mesenchymal stem cells (MSCs), known for their proliferative capacity and ability to regenerate, have shown promise in treating various diseases including tendon injury. They can differentiate into specific cells and secrete biologically active molecules to promote tissue repair and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Enzo Manchon, Nell Hirt, Jean-David Bouaziz, Nabila Jabrane-Ferrat, Reem Al-Daccak
Summary: Endosome-derived small extracellular vesicles (EVs), also known as exosomes, play a critical role in intercellular communication and have therapeutic potential in regenerative medicine. Stem cell-derived EVs are effective in repair and regeneration, with the human placenta offering a promising allogeneic stem cell source. Advancements in cell-free therapeutics provide new hope and perspectives for safe and effective treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Wenwen Zhang, Tingyu Wang, Yuanye Xue, Bingbing Zhan, Zengjie Lai, Wenjie Huang, Xinsheng Peng, Yanfang Zhou
Summary: There is increasing evidence that mesenchymal stem cell-derived extracellular vesicles and exosomes can effectively improve the treatment of oxidative stress-related diseases. These vesicles and exosomes are rich in bioactive molecules and have various biological regulatory functions. They can reduce oxidative stress and inflammation markers in systemic diseases and treat apoptosis and vascular injury caused by oxidative stress.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.
Article
Biochemistry & Molecular Biology
Shih-Heng Chen, Zhi-Yu Chen, Ya-Hsuan Lin, Shih-Hsien Chen, Pang-Yun Chou, Huang-Kai Kao, Feng-Huei Lin
Summary: This study demonstrated that extracellular vesicles (EVs) derived from adipose-derived stem cells (ADSCs) significantly enhanced tendon regeneration by promoting proliferation and migration of tenocytes. Treatment with ADSC-EVs also improved the mechanical strength of repaired tendons, suggesting a potentially highly potent therapeutic strategy for tendon injuries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Kai Song, Tao Jiang, Pin Pan, Yao Yao, Qing Jiang
Summary: This study investigates the effects of exosomes from tendon derived stem cells (TDSC-Exos) on tendon repair and explores the underlying mechanism involving microRNAs (miRNAs). The results show that TDSC-Exos can enhance tendon repair through the regulation of miR-144-3p, which promotes tenocyte proliferation and migration. This suggests that TDSC-Exos could be a promising strategy for treating tendon injuries.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Xinchi Qin, Jia He, Xiaoxiang Wang, Jingru Wang, Ronghua Yang, Xiaodong Chen
Summary: Wound repair is a complex problem and current approaches have limitations. Cell-free therapy, particularly MSC-Exos, has shown advancements in wound healing. MSC-Exos, small vesicles discharged by MSCs, contain bioactive constituents that regulate biological functions and promote wound healing. This article provides a comprehensive survey on the function and mechanisms of MSC-Exos in wound healing, emphasizing their beneficial impact on various processes.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Jiaxuan Zou, Weinan Yang, Wushi Cui, Congsun Li, Chiyuan Ma, Xiaoxiao Ji, Jianqiao Hong, Zihao Qu, Jing Chen, An Liu, Haobo Wu
Summary: Tendon-bone insertion (TBI) injuries, such as anterior cruciate ligament injury and rotator cuff injury, often require surgical treatment. Mesenchymal stem cells (MSCs) have shown therapeutic potential in promoting tissue repair and regeneration. MSCs exert their effects through the release of extracellular vesicles called exosomes, which transfer bioactive molecules to recipient cells, influencing their physiological and pathological processes. In this article, the prospective applications of MSC-derived exosomes in TBI injuries are discussed, along with the roles and underlying mechanisms of MSC-extracellular vesicles in promoting tendon-bone healing. The current challenges and future research directions are also explored.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Orthopedics
Zhuo Chen, Mingchao Jin, Hongyi He, Jiabao Dong, Jing Li, Jiangbo Nie, Zichen Wang, Juntao Xu, Fengfeng Wu
Summary: Tendon-bone insertion injuries (TBI) such as ACL and rotator cuff injuries have a negative impact on daily life and cause economic losses. The healing process is complex and involves macrophages and MSCs. MSCs respond to inflammation and can differentiate into different tissues, promoting the reconstruction of the enthesis. This review discusses the roles of macrophages and MSCs in TBI injury and healing.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Biotechnology & Applied Microbiology
Lifeng Ge, Kangyan Wang, Hang Lin, Endong Tao, Weijie Xia, Fulin Wang, Cong Mao, Yongzeng Feng
Summary: The tissue reconstruction of diabetic wounds is challenging due to severe inflammation and other complications caused by diabetes. In this study, engineered exosomes derived from miR-132-overexpressing adipose stem cells were used to promote diabetic wound healing. The miR-132-exo significantly enhanced skin flap survival and improved wound healing by reducing inflammation, promoting angiogenesis, and stimulating M2-macrophage polarization mediated by the NF-kappa B signaling pathway.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)