Article
Chemistry, Medicinal
Luigi Russo, Fabiola Mascanzoni, Biancamaria Farina, Amalia Mihaela Dolga, Alessandra Monti, Andrea Caporale, Carsten Culmsee, Roberto Fattorusso, Menotti Ruvo, Nunzianna Doti
Summary: Blocking the interaction between AIF and CypA using AIF fragment AIF(370-394) or its shorter variant AIF(381-389) is protective against glutamate-induced neuronal cell death and brain injury. The structural characterization of AIF(381-389) reveals a beta-hairpin conformation with reduced flexibility, leading to improved antiapoptotic activity and enhanced proteolytic stability compared to the parent peptide. This study provides insights for designing AIF/CypA inhibitors with better pharmacokinetic and pharmacodynamics features based on the 3D model of the AIF(381-389)/CypA complex obtained through NMR.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Dinah Farhanah Jamal, Quratul Ain Rozaimee, Nadila Haryani Osman, Atikah Mohd Sukor, Marjanu Hikmah Elias, Nor Aripin Shamaan, Srijit Das, Nazefah Abdul Hamid
Summary: This systematic review examines the role of human papillomavirus type 16 (HPV-16) early-transcribed gene product E2 protein as an apoptosis-inducer agent. All studies concluded that HPV 16-E2 protein could induce cell death in transfected cells, but the underlying mechanism remains unclear and further research is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Nicole Brimer, Scott Vande Pol
Summary: This study reveals that the enhanced competitiveness of HPV16-infected cells is primarily due to the expression of the E6 oncoprotein, rather than the E7 or E5 oncoproteins. E6 can induce a super-competitor phenotype, which has important implications for understanding the mechanism of cell competition.
Article
Biochemistry & Molecular Biology
Xiaoxu Cui, Chengyu Hao, Lijing Gong, Naoko Kajitani, Stefan Schwartz
Summary: This study reveals that hnRNP D acts as a splicing inhibitor of HPV16 E1 and E6 mRNAs, resulting in the generation of intron-containing E1 and E6 mRNAs, respectively. HnRNP D interacts with splicing machinery components and HPV16 RNA to suppress the translation of HPV16 E1 and E6 mRNAs. The association between hnRNP D and HPV16 mRNA in HPV16-driven cancer cells suggests that hnRNP D plays significant roles in the regulation of HPV gene expression and HPV-associated cancer development.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Medicine, Research & Experimental
Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, Michael Renard, Yves Jacob, Jean-Claude Twizere, Raphael Peiffer, Olivier Peulen, Philippe Delvenne, Pascale Hubert, Alison McBride, Nicolas Gillet, Murielle Masson, Michael Herfs
Summary: HPV-positive cancers are usually associated with a favorable outcome due to high sensitivity to radiation therapy. However, the impact of viral E6/E7 oncoproteins on cellular radiosensitivity and host DNA repair is still largely speculative.
Review
Biochemistry & Molecular Biology
Kakali Bhadra
Summary: This article reviews the alternative pathways of cancer cell death targeted by anticancer drug molecules, providing a molecular basis for new strategies in cancer treatment. Many drugs can effectively induce cell death through targeting the caspase-independent cell death pathway, which is significant for cancer therapy.
Review
Cardiac & Cardiovascular Systems
Liang Zong, Zhou Liang
Summary: This article summarizes the important role of apoptosis-inducing factor (AIF) in metabolic diseases, including diabetes, obesity, metabolic syndrome, and tumor metabolism. AIF mediates metabolic regulation through apoptosis. The article reviews the literature and concludes that AIF has significant functions and potential therapeutic targets in metabolic regulation.
CARDIOVASCULAR DIAGNOSIS AND THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Xuan-zhong Wang, Shi-peng Liang, Xi Chen, Zhen-chuan Wang, Chen Li, Chun-sheng Feng, Shan Lu, Chuan He, Yu-bo Wang, Guang-fan Chi, Peng-fei Ge
Summary: This study investigates the mechanism of DPT-induced parthanatos in glioma cells. The results show that over-activated PARP1 induces nuclear translocation of AIF through the downstream signal of TAX1BP1 in DPT-triggered glioma cell parthanatos.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Biochemistry & Molecular Biology
Seo Jeong Jeon, Kwang Chul Chung
Summary: This study demonstrates that tAIF is a target of ISGylation, which increases its stability and nuclear translocation, promoting apoptotic cell death. It highlights the novel role of posttranslational ISG15 modification in regulating tAIF-mediated apoptosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yan Hu, Ya-Ping Liang, Hang-Yu Tian, Cui-Xian Xu, Dan Yu, Pan Zhang, Hui Ye, Ming Li
Summary: This study investigates the role of cyclophilin A (CypA) and apoptosis-inducing factor (AIF) in the apoptosis induced by Microplitis bicoloratus bracovirus (MbBV). The findings demonstrate that the CypA-AIF interaction plays a crucial role in MbBV-induced innate immune suppression and provide insights into the understanding of insect immunological mechanisms.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Microbiology
Wen Yin, Nagayasu Egawa, Ke Zheng, Heather Griffin, Pu Tian, Ademola Aiyenuro, Jacob Bornstein, John Doorbar
Summary: Human papillomaviruses (HPV) cause persistent infections by modulating epithelial homeostasis. E6AP and NHERF1 play regulatory roles in governing epithelial homeostasis. Depletion of E6AP or expression of HPV11 or 16E6 increases keratinocyte cell density and delays differentiation. In HPV11 condyloma tissue, E6AP and NHERF1 are reduced. The preserved functions of Alpha group HPV E6 proteins modulate epithelial homeostasis via E6AP activity, altering downstream pathways.
Review
Medicine, General & Internal
Lena Wischhof, Enzo Scifo, Dan Ehninger, Daniele Bano
Summary: Apoptosis-inducing factor (AIF) is a flavoprotein located in the mitochondrial intermembrane space, playing diverse roles in cellular physiology. This review focuses on AIF's contribution to chromatin condensation during cell death and its involvement in pathogenic mechanisms of inherited metabolic diseases. The study highlights AIF's role in promoting mitochondrial oxidative phosphorylation (OXPHOS) system for cell survival and discusses its potential therapeutic implications.
Article
Oncology
John Pang, Nam Nguyen, Jens Luebeck, Laurel Ball, Andrey Finegersh, Shuling Ren, Takuya Nakagawa, Mitchell Flagg, Sayed Sadat, Paul S. Mischel, Guorong Xu, Kathleen Fisch, Theresa Guo, Gabrielle Cahill, Bharat Panuganti, Vineet Bafna, Joseph Califano
Summary: The study revealed that circular oncogenic DNA is a dominant feature in HPVOPC, with a significant portion containing human and human-viral hybrid ecDNA. The expression of these ecDNA has a specific viral oncogene expression pattern that plays a crucial role in driving oncogenic transformation and immune evasion.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Wenjun Yuan, Shuying Li, Jun Jia, Li Wang, Yuchao Huang, Minglian Wang, Fei Xie, Jintao Li, Yanzhe Hao
Summary: This study found that HPV infection, especially HPV16 and HPV18, may be a risk factor for esophageal cancer in the Chinese population. The E6 protein may play a key role in HPV-associated malignancies. These findings are important for the prevention and treatment of HPV-positive esophageal cancer in China.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Jin Bo Tang, Ya Fang Wu, Yi Cao, Chuan Hao Chen, You Lang Zhou, Bella Avanessian, Masaru Shimada, Xiao Tian Wang, Paul Y. Liu
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Masaki Shoji, Shinji Yoshizaki, Hiroyuki Mizuguchi, Kenji Okuda, Masaru Shimada
Article
Multidisciplinary Sciences
Masaru Shimada, Shinya Abe, Toru Takahashi, Kazumasa Shiozaki, Mitsue Okuda, Hiroaki Mizukami, Dennis M. Klinman, Keiya Ozawa, Kenji Okuda
Article
Immunology
Masaru Shimada, Shinji Yoshizaki, Motohide Ichino, Dennis M. Klinman, Kenji Okuda
Review
Immunology
Takehiro Ura, Akio Yamashita, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
Summary: The pandemic has accelerated vaccine development; new vaccine platforms and immunological endpoints are crucial for vaccine efficacy evaluation; updated information on individual immunological features guides effective vaccine development strategies.
Article
Immunology
Saori Ito, Jutaro Nakamura, Michiko Fukuta, Takehiro Ura, Takeshi Teshigawara, Jun Fukushima, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
Summary: This study demonstrated that vaccination with P. aeruginosa MVs and topical application of P. aeruginosa MV-specific immune sera can attenuate P. aeruginosa keratitis, significantly reducing corneal clinical score and bacterial load.
Article
Immunology
Michiko Fukuda, Jutaro Nakamura, Saori Ito, Kenji Kawazoe, Yoshitaka Miyanaga, Takeshi Teshigawara, Kenji Okuda, Nobuhisa Mizuki, Masaru Shimada
Summary: By vaccinating mice with an Ad5 vector, an adaptive immune response was successfully induced to prevent Ad transduction in corneal and conjunctiva-associated tissues. Both cellular and humoral immunity play important roles in preventing Ad transduction.
Review
Immunology
Takehiro Ura, Kenji Okuda, Masaru Shimada
Review
Immunology
Kenji Okuda, Yoshiyuki Wada, Masaru Shimada