Article
Oncology
Aleksei A. Stepanenko, Anastasiia O. Sosnovtseva, Marat P. Valikhov, Anastasia A. Chernysheva, Sergey A. Cherepanov, Gaukhar M. Yusubalieva, Zsolt Ruzsics, Anastasiia Lipatova, Vladimir P. Chekhonin
Summary: Ad5-delta-24-RGD is the most clinically advanced recombinant adenovirus for glioma therapy. Different fiber-modified rAds were constructed and compared, showing that Ad5/35-delta-24 and Ad5/3-delta-24 exhibited superior infectivity and cytolytic efficacy compared to Ad5-delta-24-RGD. The expression of adenoviral receptors/coreceptors and integrins did not predict the cytolytic efficacy of the fiber-modified rAds. CT-2A and GL261 glioma cells were identified as murine cell models for studying fiber chimeric rAds. Ad5/35-delta-24 armed with the immune costimulator OX40L demonstrated potential for glioblastoma treatment.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Microbiology
Kamilla Wiull, Preben Boysen, Katarzyna Kuczkowska, Lars Fredrik Moen, Harald Carlsen, Vincent G. H. Eijsink, Geir Mathiesen
Summary: Despite the long history of BCG vaccine, tuberculosis remains a deadly disease. This study explored new approaches to develop a more efficient vaccine, focusing on mucosal vaccines. The findings showed that surface-displaying vaccine candidates with tuberculosis antigen anchored to the cell membrane were more effective in stimulating immune response compared to candidates with intracellular antigen. This highlights the importance of antigen exposure and supports the potential of L. plantarum as a mucosal vaccine delivery vehicle against tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Tiffany M. Styles, Sailaja Gangadhara, Pradeep B. J. Reddy, Anusmita Sahoo, Ayalensh Shiferaw, Sarah Welbourn, Pamela A. Kozlowski, Cynthia A. Derdeyn, Vijayakumar Velu, Rama Rao Amara
Summary: This study compared the immunogenicity and efficacy of two vaccines based on optimized HIV envelope sequences in rhesus macaques. The results showed that both vaccines induced similar immune responses and provided some protection against SHIV infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Seung-Phil Shin, Kwang-Soo Shin, Jeong-Mi Lee, In-Kyung Jung, Jimo Koo, Seung-Woo Lee, Seowoo Park, Jieun Shin, Myunghwan Park, Bongju Park, Hanseul Oh, Bon-Sang Koo, Jungjoo Hong, Choong-Min Ryu, Jae-Ouk Kim, Taegwon Oh, Chang-Yuil Kang
Summary: The study presents the development of AdCLD-CoV19 platform, which can boost humoral and cellular immunity with a single vaccination, effectively preventing SARS-CoV-2. The platform demonstrates the ability to neutralize various SARS-CoV-2 variants, and the neutralizing antibody activity can be restored through booster shots.
Article
Pharmacology & Pharmacy
Kazuo Takayama, Kohei Ito, Akiko Matsui, Tomoki Yamashita, Kentaro Kawakami, Daisuke Hirayama, Wataru Kishimoto, Hiroshi Nakase, Hiroyuki Mizuguchi
Summary: In this study, gene expression profiles related to drug absorption, distribution, metabolism, and excretion were analyzed in biopsy samples from different regions of the intestine of patients with inflammatory bowel disease. The study found significant differences in gene expression levels across different regions of the intestine, particularly in drug-metabolizing enzymes and drug transporters. This information may be crucial for accurately predicting the pharmacokinetics of orally administered drugs.
DRUG METABOLISM AND DISPOSITION
(2021)
Article
Immunology
Kahori Shimizu, Fuminori Sakurai, Shunsuke Iizuka, Ryosuke Ono, Tomohito Tsukamoto, Fumitaka Nishimae, Shin-Ichiro Nakamura, Toru Nishinaka, Tomoyuki Terada, Yasushi Fujio, Hiroyuki Mizuguchi
Summary: Adenovirus vectors can cause hepatotoxicity in two phases post-administration, with early phase involving inflammatory cytokines. The modified Ad vector, Ad-E4-122aT-CAL2, significantly suppresses leaky Ad gene expression in the liver, leading to reduced acute hepatotoxicity. IL-6 induced by Ad vectors enhances leaky Ad gene expression and cytotoxicity, but this effect is attenuated by the presence of Ad-E4-122aT-CAL2.
JOURNAL OF IMMUNOLOGY
(2021)
Review
Immunology
Takehiro Ura, Akio Yamashita, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
Summary: The pandemic has accelerated vaccine development; new vaccine platforms and immunological endpoints are crucial for vaccine efficacy evaluation; updated information on individual immunological features guides effective vaccine development strategies.
Article
Medicine, Research & Experimental
Moe Ichikawa, Ryosuke Negoro, Kanae Kawai, Tomoki Yamashita, Kazuo Takayama, Hiroyuki Mizuguchi
Summary: The study demonstrated that vinblastine treatment can effectively remove residual undifferentiated hiPSCs in hiPSC-IECs, leading to decreased pluripotent marker expression and increased intestinal marker expression. The viability of undifferentiated hiPSCs was significantly reduced by vinblastine treatment. MDR1-negative cells, such as undifferentiated hiPSCs, were found to die upon vinblastine treatment due to their inability to excrete the drug, suggesting that vinblastine-treated hiPSC-IECs could be a safe and effective cell source for regenerative medicine.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Oncology
Ryosuke Ono, Kosuke Takayama, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: The study developed a novel OAd fully composed of OAd35, which recognizes CD46 as an infection receptor and showed efficient cell lysis activities similar to OAd5 in CAR-positive tumor cells, while exhibiting higher levels of cell lysis activities than OAd5 in CAR-negative tumor cells. OAd35 had lower proportions of neutralizing antibodies in adults compared to Ad5.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Oncology
Fuminori Sakurai, Fumitaka Nishimae, Kosuke Takayama, Hiroyuki Mizuguchi
Summary: This study examined five types of oncolytic adenoviruses (OAds) containing different types of E1 gene expression cassettes, and found that OAd-Delta 24, with a 24-bp deletion in the E1A gene, demonstrated the most efficient oncolytic activities against human tumor cell lines, despite slightly higher cytotoxicity to normal human cells compared to other OAds. These results provide important insights for the development of safe and effective OAds.
ANTICANCER RESEARCH
(2021)
Article
Immunology
Saori Ito, Jutaro Nakamura, Michiko Fukuta, Takehiro Ura, Takeshi Teshigawara, Jun Fukushima, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
Summary: This study demonstrated that vaccination with P. aeruginosa MVs and topical application of P. aeruginosa MV-specific immune sera can attenuate P. aeruginosa keratitis, significantly reducing corneal clinical score and bacterial load.
Article
Immunology
Michiko Fukuda, Jutaro Nakamura, Saori Ito, Kenji Kawazoe, Yoshitaka Miyanaga, Takeshi Teshigawara, Kenji Okuda, Nobuhisa Mizuki, Masaru Shimada
Summary: By vaccinating mice with an Ad5 vector, an adaptive immune response was successfully induced to prevent Ad transduction in corneal and conjunctiva-associated tissues. Both cellular and humoral immunity play important roles in preventing Ad transduction.
Article
Virology
Chieko Inoue, Ryosuke Negoro, Kazuo Takayama, Hiroyuki Mizuguchi, Fuminori Sakurai
Summary: This study demonstrates that hiPS-SIECs are a promising in vitro model for evaluating virus infection, with differences observed in replication and immune responses of reovirus in these cells under different infection methods.
Article
Chemistry, Analytical
Menglu Li, Yukiko Ueyama-Toba, Matthew Lindley, Gunganist Kongklad, Yasunori Nawa, Yasuaki Kumamoto, Seiichi Ishida, Yasunari Kanda, Satoshi Fujita, Hiroyuki Mizuguchi, Katsumasa Fujita
Summary: To promote the clinical application of hiPSC-derived hepatocytes, a label-free Raman microscopy method is used to monitor regenerative processes and assess differentiation efficiency. The method has successfully identified intracellular biomolecules in live samples and compared the phenotypes of hiPSC-derived hepatocytes with HepaRG and commercially available iCell hepatocytes. The study also explored the use of Raman imaging as a hepatotoxicity assay, demonstrating its potential for quality control and hepatotoxicity screening.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jumpei Inui, Yukiko Ueyama-Toba, Seiji Mitani, Hiroyuki Mizuguchi
Summary: A method has been developed to dissociate, cryopreserve, and reseed human induced pluripotent stem cell-derived hepatocyte-like cells (HLCs) without loss of their functions. This technology enables the availability of cryopreserved HLCs for drug discovery research.
Article
Biology
Eiko Sakai, Tsutomu Imaizumi, Ruruka Suzuki, Marcos Taracena-Gandara, Toshiki Fujimoto, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: Non-alcoholic liver disease (NAFLD) is caused by excessive fat accumulation in the liver and miR-27b has been found to play a crucial role in NAFLD development by targeting genes involved in lipid metabolism. This study identified MAIP1 as a direct target of miR-27b and showed that overexpression of MAIP1 can ameliorate lipid accumulation induced by miR-27b. Additionally, decreased Maip1 expression was observed in mice fed a high-fat diet, suggesting its involvement in fatty liver condition and its potential as a therapeutic target for NAFLD.
COMMUNICATIONS BIOLOGY
(2023)