Article
Biochemistry & Molecular Biology
Ioannis-Alexandros Gampierakis, Yassemi Koutmani, Maria Semitekolou, Ioannis Morianos, Ioannis Charalampopoulos, Georgina Xanthou, Achille Gravanis, Katia P. Karalis
Summary: This study investigates the effects of acute and chronic experimental colitis on adult hippocampal neurogenesis and innate immune cell responses. It reveals that acute colitis enhances neurogenesis but with deficits in cell cycle kinetics, while chronic colitis shows normal levels of neurogenesis but deficits in neuron migration and integration. Additionally, acute colitis leads to increased inflammation in the hippocampus, while chronic colitis results in elevated levels of tissue-repairing cells and anti-inflammatory cytokines. These findings highlight potential mechanisms underlying cognitive and mood dysfunction in patients with IBD.
MOLECULAR PSYCHIATRY
(2021)
Article
Immunology
Huldani Huldani, Ria Margiana, Fawad Ahmad, Maria Jade Catalan Opulencia, Mohammad Javed Ansari, Dmitry Olegovich Bokov, Nargiza N. Abdullaeva, Homayoon Siahmansouri
Summary: Inflammatory bowel disease (IBD) is caused by various pathophysiologic pathways and immune-pathologic etiologies. Mesenchymal Stem Cells (MSCs) have been suggested as a potential treatment for IBD due to their immune regulatory functions. Clinical trials using MSCs from bone marrow, umbilical cord, and adipose tissue have shown promising results in terms of safety. However, further research is needed to obtain more reliable results and more support from laboratory evidence.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tyler Atagozli, David E. Elliott, Mirac Nedim Ince
Summary: Helminths are multicellular invertebrates that live in the gut of vertebrate animals, including humans. They can cause pathology and require treatment, but can also have commensal or symbiotic relationships with their hosts. Epidemiological data suggests that helminth exposure may provide protection against immune disorders, such as allergies and autoimmune illnesses.
Review
Medicine, General & Internal
Aleksandra Kubas, Ewa Malecka-Wojciesko
Summary: Vaccination against COVID-19 is considered safe and effective for patients with IBD, although further research is needed to understand its safety in special populations. The willingness to receive the vaccine among IBD patients is conflicting, with concerns about effectiveness and safety being the most common reasons for hesitancy.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Chao Liu, Yuhong Jiang, Ganglei Liu, Zhushu Guo, Qianqian Jin, Dongju Long, Weihan Zhou, Ke Qian, Hua Zhao, Kuijie Liu
Summary: This study found that PPARGC1A was downregulated in DSS-induced colitis and DSS-stimulated colonic epithelial cells, and PDT treatment could upregulate PPARGC1A expression. Knockdown of PPARGC1A could attenuate the therapeutic effects of PDT on DSS-induced colitis and DSS-stimulated colonic epithelial cells.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Andrea Cassinotti, Francesco Passamonti, Sergio Segato
Summary: Cell-based therapies have been explored in immune-mediated inflammatory diseases, including inflammatory bowel disease (IBD). The most studied treatment in IBD involves stem cells, with recent studies also focusing on regulatory cells such as T-reg and Tr1 cells. Preclinical research is also being conducted on induced pluripotent stem cells in animal models related to colitis.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Shuxia Zhang, Yujie Liang, Jun Yao, De-feng Li, Li-sheng Wang
Summary: Pyroptosis is an inflammatory cell death process executed by gasdermin proteins, which plays a role in mucosal innate immunity, enteropathogenic bacterial infection, and regulation of intestinal inflammation. It initiates damage signals that activate multiple pathways to cause inflammation, potentially contributing to chronic intestinal inflammation.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Hua-Min Zhang, Shuo Yuan, Huan Meng, Xiao-Ting Hou, Jiao Li, Jia-Chen Xue, You Li, Qi Wang, Ji-Xing Nan, Xue-Jun Jin, Qing-Gao Zhang
Summary: Inflammatory bowel disease (IBD) is a chronic, relapsing disease that greatly affects patients' quality of life. Stem cell therapy has shown potential to improve the clinical disease activity of patients with refractory IBD when conventional treatments are not effective, but its safety and long-term efficacy need further evaluation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Alun Vaughan-Jackson, Szymon Stodolak, Kourosh H. Ebrahimi, Errin Johnson, Paul K. Reardon, Maeva Dupont, Shengpan Zhang, James S. O. McCullagh, William S. James
Summary: This study reveals that the density of macrophage culture can influence their morphology, phenotype, and inflammatory response. These density-dependent changes are observed in various cell types and are driven by a transferable soluble factor. This finding emphasizes the importance of considering cell plating density in in vitro macrophage research.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Mirae Lee, Eugene B. Chang
Summary: Inflammatory bowel diseases (IBD) result from the interaction of environmental, microbial, immunological, and genetic factors, with alterations in the gut microbiota playing a role in their development and progression. The use of microbiome-based therapies can potentially prevent, manage, and cure IBD, bridging the gap between basic sciences and clinical application through research findings.
Review
Medicine, Research & Experimental
Mohamed J. Saadh, Maria V. Mikhailova, Soheil Rasoolzadegan, Mojgan Falaki, Roozbeh Akhavanfar, Jose Luis Arias Gonzales, Amir Rigi, Bahman Abedi Kiasari
Summary: Introduces the emerging therapeutic modality of mesenchymal stem/stromal cells (MSCs) therapy for the treatment of inflammatory bowel disease (IBD). MSCs alleviate dysregulated inflammatory responses through the secretion of anti-inflammatory mediators and their effects on gut immuno-microbiota regulation and the proliferation and differentiation of intestinal cells. Clinical trials are being conducted to evaluate the safety and efficacy of MSCs administration in IBD patients.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Eleonora Gori, Alessio Pierini, Martina Nesci, Elena Benvenuti, Silvia Tasca, George Lubas, Veronica Marchetti
Summary: This study aimed to investigate the presence of anti-RBC antibodies and potential red blood cell abnormalities in dogs with chronic intestinal inflammation (IBD). Results showed that a high percentage of dogs with IBD had anti-RBC antibodies present, suggesting possible subclinical chronic immune-mediated hemolysis. Additional findings of red blood cell regeneration were also observed in some dogs, indicating potential hemolytic processes at play in canine IBD.
Article
Oncology
Zoe Schmal, Ben Hammer, Andreas Mueller, Claudia E. Ruebe
Summary: Despite advances in hippocampus-sparing radiation therapy, radiation-induced injury to the neural stem cell compartment may impact neurocognitive functions. Low doses of ionizing radiation induced inflammatory responses in the hippocampus, with activated microglia and reactive astrocytes, suggesting a shift from neurogenesis to gliogenesis. These findings emphasize the importance of limiting radiation dose to preserve neurocognitive functions, particularly in the juvenile hippocampus.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Pediatrics
Maya Ruban, Adam Slavick, Achiya Amir, Amir Ben-Tov, Hadar Moran-Lev, Yael Weintraub, Adi Anafy, Shlomi Cohen, Anat Yerushalmy-Feler
Summary: The rate of a positive family history of IBD in the pediatric IBD population is increasing, and patients with a positive family history are more likely to have a severe phenotype, receive more nutritional therapy, and less corticosteroids. Patients with a negative family history may require intensified biologic therapy.
EUROPEAN JOURNAL OF PEDIATRICS
(2022)
Article
Cell Biology
Xin Tang, Ellen Walter, Eric Wohleb, Yanbo Fan, Chenran Wang
Summary: Microglial autophagy plays a critical role in postnatal hippocampal neurogenesis in an AD mouse model, particularly in female mice.
Article
Biochemistry & Molecular Biology
Ioannis-Alexandros Gampierakis, Yassemi Koutmani, Maria Semitekolou, Ioannis Morianos, Ioannis Charalampopoulos, Georgina Xanthou, Achille Gravanis, Katia P. Karalis
Summary: This study investigates the effects of acute and chronic experimental colitis on adult hippocampal neurogenesis and innate immune cell responses. It reveals that acute colitis enhances neurogenesis but with deficits in cell cycle kinetics, while chronic colitis shows normal levels of neurogenesis but deficits in neuron migration and integration. Additionally, acute colitis leads to increased inflammation in the hippocampus, while chronic colitis results in elevated levels of tissue-repairing cells and anti-inflammatory cytokines. These findings highlight potential mechanisms underlying cognitive and mood dysfunction in patients with IBD.
MOLECULAR PSYCHIATRY
(2021)
Article
Oncology
Eleni Skavatsou, Maria Semitekolou, Ioannis Morianos, Theodoros Karampelas, Nikolaos Lougiakis, Georgina Xanthou, Constantin Tamvakopoulos
Summary: The combination of metronomic chemotherapy with immunotherapy shows potential efficacy and tolerability in the treatment of non-small cell lung cancer, providing new options for patients with reduced toxicities.
Article
Oncology
Ioannis Morianos, Aikaterini Tsitsopoulou, Konstantinos Potaris, Dimitrios Valakos, Ourania Fari, Giannis Vatsellas, Clementine Bostantzoglou, Andreas Photiades, Mina Gaga, Georgina Xanthou, Maria Semitekolou
Summary: This study reveals that activin-A can activate exhausted CD4(+) T cells in the lung tumor microenvironment, enhancing their anti-tumor potential. Treatment with activin-A delays tumor progression in lung cancer-bearing mice and boosts T cell-mediated immune responses.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Allergy
Efthymia Theofani, Maria Semitekolou, Konstantinos Samitas, Annie Mais, Ioanna E. Galani, Vasiliki Triantafyllia, Joanna Lama, Ioannis Morianos, Athanasios Stavropoulos, Se-Jin Jeong, Evangelos Andreakos, Babak Razani, Nikoletta Rovina, Georgina Xanthou
Summary: This study investigates the effects of autophagy and its master regulator, transcription factor EB (TFEB), on monocyte responses in asthmatic individuals.
Article
Biochemistry & Molecular Biology
Canelif Yilmaz, Thanasis Rogdakis, Alessia Latorrata, Evangelia Thanou, Eleftheria Karadima, Eleni Papadimitriou, Eleni Siapi, Ka Wan Li, Theodora Katsila, Theodora Calogeropoulou, Ioannis Charalampopoulos, Vasileia Ismini Alexaki
Summary: The newly synthesized small molecule ENT-A010 exhibited neuroprotective effects and modulated microglial function through the activation of the TRKA receptor signaling pathway, showing potential in the treatment of CNS disorders.
Article
Biochemistry & Molecular Biology
Thanasis Rogdakis, Despoina Charou, Alessia Latorrata, Eleni Papadimitriou, Alexandros Tsengenes, Christina Athanasiou, Marianna Papadopoulou, Constantina Chalikiopoulou, Theodora Katsila, Isbaal Ramos, Kyriakos C. Prousis, Rebecca C. Wade, Kyriaki Sidiropoulou, Theodora Calogeropoulou, Achille Gravanis, Ioannis Charalampopoulos
Summary: A new NGF mimetic, ENT-A013, has been identified to selectively activate TrkA receptor and exert neuroprotective effects, showing potential for therapeutic development against neurodegenerative disorders, such as Alzheimer's Disease.
Article
Biochemistry & Molecular Biology
Alexandros Tsimpolis, Maria Kokkali, Aris Logothetis, Konstantinos Kalafatakis, Ioannis Charalampopoulos
Summary: Novel research studies have discovered complex interactions between glucocorticoid hormones and brain-derived neurotrophic factor, regulating crucial physiological processes in neuronal cells. This study investigated the acute effects of glucocorticoid stimulation on gene expression of BDNF and TrkB in astrocytes, revealing a biphasic response characterized by an initial rapid upregulation and subsequent downregulation of BDNF mRNA under the influence of glucocorticoid receptors. These findings demonstrate a rhythmic regulation of the neurotrophin system based on glucocorticoid levels, indicating the intricate trophic responses to temporal hormonal mechanisms in the brain microenvironment.
Article
Chemistry, Medicinal
Mirjana Antonijevic, Despoina Charou, Isbaal Ramos, Maria Valcarcel, Achille Gravanis, Patricia Villace, Noelle Callizot, Marc Since, Patrick Dallemagne, Ioannis Charalampopoulos, Christophe Rochais
Summary: Numerous studies have investigated the role of BDNF and its receptor TrkB in neurodegenerative diseases. BDNF activates the TrkB receptor, promoting neuronal survival and differentiation. In this study, a small data set of compounds was designed and synthesized based on the structure of a previously published activator of TrkB. The compounds were evaluated for their ADME and toxicity profiles, as well as their biological properties and affinity to promote neurite differentiation through the TrkB receptor.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Maria Kannavou, Kanelina Karali, Theodora Katsila, Eleni Siapi, Antonia Marazioti, Pavlos Klepetsanis, Theodora Calogeropoulou, Ioannis Charalampopoulos, Sophia. G. G. Antimisiaris
Summary: Intranasal administration provides a promising method for direct nose-to-brain drug delivery. In this study, chitosan-coated nanoformulations of BNN27 were developed and compared with uncoated liposomes and nanoemulsions in terms of properties and brain disposition. The results showed that chitosan-coated nanoemulsions demonstrated higher and faster brain disposition of BNN27 following intranasal administration compared to chitosan-coated liposomes.
Article
Cell Biology
Evangelia Thanou, Frank Koopmans, Debora Pita-Illobre, Remco V. V. Klaassen, Berna Ozer, Ioannis Charalampopoulos, August B. B. Smit, Ka Wan Li
Summary: sTRAP is an effective sample preparation method for proteomics studies, using 5% SDS for protein solubilization and a borosilicate glass membrane filter to trap proteins. Compared to other methods, sTRAP outperforms in terms of protein and peptide identification numbers and coefficient of variation. sTRAP was successfully applied to analyze the hippocampal proteome in a mouse model of Alzheimer's disease, revealing changes in proteins related to the immune system and Amyloid aggregation.
Article
Biochemistry & Molecular Biology
Maria Anna Papadopoulou, Thanasis Rogdakis, Despoina Charou, Maria Peteinareli, Katerina Ntarntani, Achille Gravanis, Konstantina Chanoumidou, Ioannis Charalampopoulos
Summary: Neuronal cell fate is controlled by growth factors and their receptors Trk and p75NTR. Synthetic analogs like ENT-A044 can mimic neurotrophins without side effects. ENT-A044 can induce cell death and activate both p75NTR and TrkB receptors, providing a potential therapeutic approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Paolo Giaccio, Despoina Charou, Dafni-Ioanna Diakaki, Anna Chita, Achille Gravanis, Ioannis Charalampopoulos, Vassilios Roussis, Efstathia Ioannou
Summary: Neurodegenerative diseases are incurable and debilitating conditions. Currently, effective therapies for most neurodegenerative disorders are lacking. A study evaluated the activity of various extracts on TrkB-expressing cells and found that a certain strain of actinobacteria called Streptomyces sp. BI0788 exhibited neuroprotective activity. Further chemical analysis resulted in the identification of new and previously known compounds, some of which showed protective effects on cells through the activation of TrkB.
