Article
Chemistry, Multidisciplinary
Shih-Wei Wu, Jiunn-Liang Ko, Biing-Hui Liu, Feng-Yih Yu
Summary: A commercial kit and immunostrip for screening CTN in red yeast rice were established in this study. The sensitivity and specificity of the kit were optimized, and the stability of the components was evaluated. Analysis of red yeast rice samples using the kit revealed high levels of CTN contamination, and rapid detection with the immunostrip showed consistent results.
Article
Biochemical Research Methods
Jingming Zhou, Qingbao Yang, Chao Liang, Yumei Chen, Xiaoli Zhang, Zhanxiang Liu, Aiping Wang
Summary: An efficient method for the detection of OTA in corn, utilizing a quantum dots-based fluorescent immunochromatographic assay (QDs-ICA), exhibited excellent specificity and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chen Chen, Jiao Cao, Xiaochen Wang, Qian Chai, Yunjuan Zhang, Hongzhong Chen, Xiaohui Zhu, Yun Pan, Jinliang Liu
Summary: Mycotoxins, secondary metabolites of fungi, pose a serious threat to human health. A novel dual-flux immunochromatographic assay (dICA) using upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs) was developed for simultaneous quantitative detection of ochratoxin A (OTA) and deoxynivalenol (DON). The dICA provided a sensitive, rapid, and reliable on-site simultaneous detection of multiple mycotoxins.
Article
Chemistry, Multidisciplinary
Lu Lin, Xinxin Xu, Shanshan Song, Liqiang Liu, Hua Kuang, Zhengyou Wang, Chuanlai Xu
Summary: In this study, an immunochromatographic strip (ICS) was developed for the specific detection of AZX in vegetable samples, using a highly specific and sensitive monoclonal antibody (mAb). The ICS showed qualitative detection within 10 minutes and quantitative detection with linear regression equations, IC50 values, LODs, and linear detection ranges for cucumber, tomato, and cabbage samples. The results showed that the ICS could be a rapid and accurate tool for the detection of AZX in vegetable samples.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Shengyang Zhou, Liguang Xu, Hua Kuang, Jing Xiao, Chuanlai Xu
Summary: A rapid lateral flow fluorescence microsphere immunoassay test strip (FM-ICTS) was established for the detection of deoxynivalenol (DON) residues in agricultural products based on competitive immunoreactivity. The method, utilizing highly sensitive antibodies and fluorescent markers, is simple, reliable, and sensitive for food safety analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Veterinary Sciences
Jingneng Wang, Lihua Wang, Hui Zhang, Xinglin Mei, Liangzhu Qiu, Jing Liu, Yongsong Zhou
Summary: Through best management practices, the risk of deoxynivalenol (DON) contamination in food crops and feeds can be effectively managed and mitigated. A DON test strip based on time-resolved fluorescence immunoassay (TRFIA) and a specific DON monoclonal antibody was developed for rapid quantification of DON in food crops and feeds. The strip showed good linearity and high accuracy, making it suitable for on-site and laboratory determination of DON.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Yue Li, Jiahao Li, Huachuan Huang, Dan Jian, Yanke Shan, Shouyu Wang, Fei Liu
Summary: The HADS system utilizes LFIA strips and a handheld sensor reader to quickly detect antibiotic residues in honey with high sensitivity and good specificity. LFIA strips can be stored long-term in vacuum-sealed bags and quantitatively measured automatically using the handheld sensor reader.
Article
Chemistry, Applied
Jiadi Sun, Miao Li, Fuguo Xing, Haiming Wang, Yinzhi Zhang, Xiulan Sun
Summary: This study developed a dual immunochromatographic test strips method based on double antibodies labeled with time resolved fluorescent microspheres to realize simultaneous rapid detection of AFM1 and OTA in milk. The method showed low detection limits and good accuracy, repeatability, and specificity.
Article
Computer Science, Artificial Intelligence
Nianyin Zeng, Han Li, Zidong Wang, Weibo Liu, Songming Liu, Fuad E. Alsaadi, Xiaohui Liu
Summary: This paper presents a novel image segmentation method based on deep reinforcement learning for quantitative analysis of Gold Immunochromatographic Strips (GICS). Experimental results demonstrate that the proposed method outperforms existing image segmentation methods in distinguishing between the test line and control line on GICS images.
Article
Chemistry, Applied
Koji Kikukawa, Miki Fukui, Ayano Oka, Ryohei Yamamoto, Atsushi Yamamoto, Mikiko Uchigashima, Takeshi Kono, Daisuke Kozaki, Shuji Kodama
Summary: A method for directly measuring mycotoxin ochratoxin A (OTA) in foods was established by solid-phase fluorescence of monolith-immobilized antibodies, showing good linearity and sensitivity. The proposed method, applied to solid samples with complex matrices, achieved high recovery rates.
Article
Nutrition & Dietetics
Hong Shen, Yuping Wan, Xiaosheng Wu, Yu Zhang, Jingwen Li, Tingting Cui, Han Sun, Haifeng Cui, Kailun He, Guangpeng Hui, Xu Chen, Guoqiang Liu, Meihong Du
Summary: This study successfully synthesized two haptens using rational design and obtained anti-aldicarb monoclonal antibodies through immunization of mice. A colloidal gold immunochromatographic strip was developed, which provides a rapid and accurate method for detecting aldicarb in agricultural products and environments, with high sensitivity and low cross-reactivity.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemical Research Methods
Yuan Cheng, Lingling Guo, Aihong Wu, Xinxin Xu, Liqiang Liu, Chuanlai Xu, Hua Kuang, Liguang Xu
Summary: In this study, a hapten was designed and coupled with a carrier protein to synthesize an immunogen and coating antigen. A monoclonal antibody was fabricated and assessed for its performance. A colloidal gold nanoparticle immunochromatographic test strip (CGN-ICTS) was developed and shown to have high sensitivity for detecting tolfenpyrad residues in food samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Chong Yu, Yanwu Wei, Hao Zhang, Jianhang Liu, Li Feng, Changming Liu, Liping Huang
Summary: A rapid and specific antigen detection method for porcine circovirus type 2 (PCV2) was established using monoclonal antibodies and a red latex microsphere immunochromatographic strip. The method showed good sensitivity, specificity, and ease of use, making it valuable for on-site diagnosis and virus quantification of PCV2.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Beijun Ang, Xinxin Xu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu
Summary: The study developed monoclonal antibodies against Shigella and produced a lateral flow immunochromatographic (LFIA) test strip method for detecting various serotypes of Shigella. The test strip showed sensitivity for four groups and seven serotypes of Shigella, and was able to detect Shigella in spiked milk and meat samples.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuan Cheng, Wenliang Ge, Hua Kuang, Jianping Zhu, Liqiang Liu, Yingyue Zhu, Chuanlai Xu
Summary: A highly sensitive and specific monoclonal antibody was developed for KCZ detection in chicken samples, with a visual limit of detection of 0.5 ng/g. The CGIA method shows great potential for rapidly detecting KCZ residues in chicken samples, with results revealed in only 10 minutes.
MICROCHEMICAL JOURNAL
(2022)
Article
Biodiversity Conservation
Guangqian Ren, Yizhou Du, Bin Yang, Jingjing Wang, Miaomiao Cui, Zhicong Dai, Michael Opoku Adomako, Susan Rutherford, Daolin Du
Summary: Over the past few decades, rainfall has become more variable, leading to increased frequency of extreme precipitation events worldwide. However, the effects of precipitation dynamics on plant invasions have been understudied. This study found that Alternanthera philoxeroides is more responsive to increased and variable water availability compared to native plants, suggesting its potential for invasive growth.
BIOLOGICAL INVASIONS
(2023)
Review
Soil Science
Babar Iqbal, Tingting Zhao, Weiqing Yin, Xin Zhao, Qingjie Xie, Kiran Yasmin Khan, Xiaoxun Zhao, Mudasir Nazar, Guanlin Li, Daolin Du
Summary: This study reviews the impacts of microplastics (MPs) on crops, both direct and indirect, as well as their combined effects with other pollutants. The findings suggest that MPs inhibit water and nutrient uptake, cause oxidative damage and disruption of metabolic activities in crops. Chemical additives released from MPs also induce cytotoxicity and genotoxicity. MPs also alter the soil environment, affecting water and nutrient availability for crops. Combined toxicity of MPs and other common soil pollutants have adverse effects on crops.
APPLIED SOIL ECOLOGY
(2023)
Article
Ecology
Shanshan Zhong, Zhelun Xu, Yue Li, Chuang Li, Youli Yu, Congyan Wang, Daolin Du
Summary: This study evaluated the co-allelopathy of two Asteraceae invasives under acid rain with different nitrogen-to-sulfur ratios. Aqueous extracts of the invasives showed obvious allelopathic effects on Lactuca sativa. Solidago canadensis extracts exhibited stronger allelopathy, while nitric acid weakened the co-allelopathy of the two invasives. Overall, the allelopathy of the invasives increased with increasing acidity of acid rain, but decreased with increasing nitrogen-to-sulfur ratio.
Article
Environmental Sciences
Kiran Yasmin Khan, Guanlin Li, Daolin Du, Barkat Ali, Shuang Zhang, Meiying Zhong, Peter Joseph Stoffella, Babar Iqbal, Xiaoqiang Cui, Lijiang Fu, Ya Guo
Summary: Antibiotics and microplastics, both emerging contaminants, have become global environmental issues, posing a major threat to human health via their application in soil and entrance in the food chain. This study investigated the effects of single and combined exposure to polystyrene microplastic, norfloxacin, and sulfadiazine on a medicinal food crop, Chrysanthemum coronarium L. The results showed that these contaminants accumulated in the roots, shoots, and leaves of the plant and affected their ultrastructure, leading to changes in nutrient element contents and leaf metabolites. The co-contamination of microplastics and antibiotics had a greater impact on plant metabolites due to the combination of multiple abiotic stresses. These findings highlight the ecological risks of microplastics to medicinal food plants and contribute to understanding the phytotoxicity mechanisms of different antibiotics in the presence of polyethylene microplastic.
ENVIRONMENTAL POLLUTION
(2023)
Review
Genetics & Heredity
Ping Huang, Rashida Hameed, Manzer Abbas, Sidra Balooch, Badr Alharthi, Yizhou Du, Adeel Abbas, Afifa Younas, Daolin Du
Summary: Many emerging invasive weeds have rapid adaptation and dispersal habits, resulting in strong diversity within their populations compared to their native counterparts. Advancements in molecular marker techniques are enhancing our understanding of the genetic diversity of invasive weeds and their implications in genome research. This review-based study examines the use of multi-omics approaches in understanding the functional, structural, and genomic changes of invasive weeds in different environmental conditions, emphasizing the accessibility of advanced sequencing techniques in genome projects.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Agronomy
Miaomiao Cui, Jingjing Wang, Xuefeng Zhang, Congyan Wang, Guanlin Li, Justin S. H. Wan, Daolin Du
Summary: This study aimed to determine the impacts of global warming and phosphorus deposition on the competition between native Artemisia argyi and invasive Solidago canadensis. The results showed that warming negatively affected the growth of both native A. argyi and invasive S. canadensis, while phosphorus deposition promoted the growth of A. argyi. The combined treatment had an inhibitory effect on the growth of both plants.
Article
Horticulture
Zhelun Xu, Shanshan Zhong, Youli Yu, Yueyang Wang, Huiyuan Cheng, Daolin Du, Congyan Wang
Summary: Most of the research on allelopathic effects has focused on invasive herbaceous plants, ignoring the allelopathic effects of invasive trees and the differences between invasive and native species. Different forms of nitrogen fertilization can influence these effects. This study aimed to evaluate the allelopathic effects of an invasive tree (Rhus typhina) and a native tree (Koelreuteria paniculata) on Lactuca sativa under different N forms, and found that R. typhina had stronger effects on the leaf photosynthetic area, plant growth competitiveness, and water content of L. sativa than K. paniculata.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Youli Yu, Shanshan Zhong, Zhelun Xu, Zhongyi Xu, Congyan Wang, Daolin Du
Summary: This study aimed to analyze the independent and co-allelopathy of two Asteraceae invasive plants on the germination and seedling growth of a horticultural species under salt stress. The results showed that salt stress strengthened the independent and co-allelopathy of the invasive plants, with greater effects observed under heavy salt stress.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Chemistry, Analytical
Ming Li, Wenying Lu, Yuhao Mao, Xuchun Qiu, Daolin Du
Summary: This study established and evaluated an enhanced ICA strip for simultaneous, semi-quantitative and quantitative detection of OTA and AFB1. The use of E. coli K12 as a carrier improved the sensitivity and detection range of the ICA. The reliability and applicability of the ICA were confirmed by its good correlation with LC-MS/MS.
Article
Forestry
Shanshan Zhong, Zhelun Xu, Huiyuan Cheng, Yueyang Wang, Youli Yu, Daolin Du, Congyan Wang
Summary: Drought stress intensifies the allelopathy of Rhus typhina and Sapindus mukorossi, with the latter experiencing a more significant increase in allelopathy. This study aimed to assess the differences in allelopathy between invasive and indigenous woody species, and their effects on the seed germination and seedling growth of Lactuca sativa under drought stress. The results showed that both Rhus typhina and Sapindus mukorossi exhibited strong allelopathy, with Rhus typhina being notably stronger. Drought stress intensified the allelopathy of both species, but had a more significant effect on Sapindus mukorossi. This suggests that drought stress can accelerate the invasion and expansion processes of plants, but the stronger allelopathy of indigenous plants may weaken the invasion process to some extent under drought stress.
TREES-STRUCTURE AND FUNCTION
(2023)
Review
Environmental Sciences
Rashida Hameed, Guanlin Li, Yowhan Son, Huajun Fang, Taewan Kim, Chaodong Zhu, Yanfang Feng, Lihua Zhang, Adeel Abbas, Xin Zhao, Jiaqian Wang, Jian Li, Zhicong Dai, Daolin Du
Summary: This review highlights the molecular and chemical compositions and structural evolution of dissolved black carbon (DBC), as well as its influence on pyrolysis, interaction with soil and contaminants, stability, and potential risks in soil and water environments. Future research should focus on understanding the chemical and molecular structure of DBC, investigating its multi-functional properties and interaction mechanisms, and evaluating the environmental behaviors and risks associated with DBC after biochar application.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Jian Li, Zhanrui Leng, Taitiya Kenneth Yuguda, Lili Wei, Jiaojiao Xia, Chongyu Zhuo, Ziying Nie, Daolin Du
Summary: Coastal reclamation is a common trend worldwide due to increasing land demand, but it poses ecological threats to coastal wetlands. This study examines the trade-offs between economic benefits and ecological degradation of invasive alien plants and seawall coastal reclamation in China. The findings show significant decline of ecosystem service value (ESV) loss from S. alterniflora reclamation, while seawalls reclamation yielded some ESV gain. However, the cumulative ESV loss outweighed the economic gains, indicating a potential long-term threat to the ecological integrity and carbon sinks in coastal wetlands.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Miaomiao Cui, Bin Yang, Guangqian Ren, Haochen Yu, Zhicong Dai, Jian Li, Qiong Ran, Piergiorgio Stevanato, Justin Wan, Daolin Du
Summary: Anthropogenic climate change and species invasion pose significant threats to biodiversity, impacting the survival and distribution of species worldwide. Understanding the responses of invasive species to climate change can provide valuable insights into the ecological and genetic mechanisms of their invasion. However, the effects of warming and phosphorus deposition on native and invasive plant phenotypes remain uncertain.
Review
Plant Sciences
Irfan Ullah Khan, Shan-Shan Qi, Farrukh Gul, Sehrish Manan, Justice Kipkorir Rono, Misbah Naz, Xin-Ning Shi, Haiyan Zhang, Zhi-Cong Dai, Dao-Lin Du
Summary: Heavy metals are released into ecosystems by human activities, posing threats to plant productivity and human health. Phytoremediation, particularly in weedy plants, is a clean and cost-effective method for reducing soil toxicity. This review discusses the mechanism of HM uptake, translocation, and detoxification in invasive plant species (IPS) and explores advanced biotechnological strategies for improving phytoremediation.
Article
Plant Sciences
Youli Yu, Huiyuan Cheng, Congyan Wang, Daolin Du
Summary: This study aimed to assess the independent and combined effects of two composite invasive alien plants (Bidens pilosa and Solidago canadensis) on litter decomposition and soil enzyme activities under drought conditions. The results showed that the co-invasion of the two species had a synergistic effect on urease activity, and the decomposition rate of mixed litters was higher than that of S. canadensis. Moreover, heavy drought reduced the decomposition rate of the two composite species and B. pilosa, while it did not affect S. canadensis litter decomposition.
JOURNAL OF PLANT ECOLOGY
(2023)
