A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris

Evolution of E. coli Phytase for Increased Thermostability Guided by Rational Parameters

(2019) Jiadi Li et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

High-level expression of β-N-Acetylglucosaminidase BsNagZ in Pichia pastoris to obtain GlcNAc

(2019) Shun Jiang et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Effect of phytase on intestinal phytate breakdown, plasma inositol concentrations, and glucose transporter type 4 abundance in muscle membranes of weanling pigs1

(2019) Hang Lu et al.   JOURNAL OF ANIMAL SCIENCE

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

(2019) Lukas Marsalek et al.   Microbial Cell Factories

Purification and kinetics of a protease-resistant, neutral, and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 ameliorating food nutrition

(2018) Jinender Jain et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Production of Phytase Enzyme by a Bioengineered Probiotic for Degrading of Phytate Phosphorus in the Digestive Tract of Poultry

(2018) Bahareh Pakbaten et al.   Probiotics and Antimicrobial Proteins

Efficient protein production by yeast requires global tuning of metabolism

(2017) Mingtao Huang et al.   Nature Communications

Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris

(2017) Lukas Marsalek et al.   Biotechnology Journal

Phytase Production and Development of an Ideal Dephytinization Process for Amelioration of Food Nutrition Using Microbial Phytases

(2016) Jinender Jain et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

CRISPR/Cas9 Immune System as a Tool for Genome Engineering

(2016) Magdalena Hryhorowicz et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

(2015) Cheng Li et al.   BMC BIOTECHNOLOGY

TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling

(2015) Isamu Sakabe et al.   BREAST CANCER RESEARCH AND TREATMENT

Molecular Characterization of Endoplasmic Reticulum Oxidoreductin 1 from Bombyx mori

(2015) Minchul Seo et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology

(2015) Ali Akbarzadeh et al.   Jundishapur Journal of Microbiology

Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

(2014) Zihe Liu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design

(2014) Tzu-Hui Wu et al.   JOURNAL OF BIOTECHNOLOGY

Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation

(2013) Ming-Ze Yao et al.   BIOTECHNOLOGY LETTERS

Enhancement of lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase

(2013) Chong Sha et al.   ENZYME AND MICROBIAL TECHNOLOGY

Overexpression of Escherichia coli Phytase in Pichia pastoris and Its Biochemical Properties

(2013) Hsueh-Ming Tai et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase

(2013) Raquel Fonseca-Maldonado et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

(2013) Min Wu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Animal production for efficient phosphate utilization: from optimized feed to high efficiency livestock

(2012) Ermias Kebreab et al.   CURRENT OPINION IN BIOTECHNOLOGY

Bleach boosting effect of xylanase A from Bacillus halodurans C-125 in ECF bleaching of wheat straw pulp

(2012) Xiao-qiong Lin et al.   ENZYME AND MICROBIAL TECHNOLOGY

Phytase, a New Life for an “Old” Enzyme

(2012) Xin Gen Lei et al.   Annual Review of Animal Biosciences

Protein secretion in Pichia pastoris and advances in protein production

(2011) Leonardo M. Damasceno et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

(2011) Graham Whyteside et al.   PLoS One

Large-scale assignment of N-glycosylation sites using complementary enzymatic deglycosylation

(2011) Wei Zhang et al.   TALANTA

β-Propeller Phytase Hydrolyzes Insoluble Ca2+-Phytate Salts and Completely Abrogates the Ability of Phytate To Chelate Metal Ions

(2010) Ok-Hee Kim et al.   BIOCHEMISTRY

Muropeptide Rescue in Bacillus subtilis Involves Sequential Hydrolysis by  -N-Acetylglucosaminidase and N-Acetylmuramyl-L-Alanine Amidase

(2010) S. Litzinger et al.   JOURNAL OF BACTERIOLOGY

Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae

(2010) Eun Young Cho et al.   JOURNAL OF BIOTECHNOLOGY

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH

Effect of glycosylation on protein folding: A close look at thermodynamic stabilization

(2008) D. Shental-Bechor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA