Review
Pharmacology & Pharmacy
Hannah C. Zierden, Rachel L. Shapiro, Kevin DeLong, Davell M. Carter, Laura M. Ensign
Summary: Preterm birth is defined as delivery before 37 weeks of gestation, with 15 million infants born prematurely globally each year, increasing the risk of mortality and health challenges. While the only FDA approved therapy for preventing preterm birth in the U.S. has faced recent setbacks, interdisciplinary efforts are underway to develop next generation therapies to prevent preterm birth and save infant lives. This research aims to better identify women at risk for preterm birth, prevent premature labor, and ultimately improve outcomes for newborns.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Akkaranunt Supakijsilp, Jing He, Xubo Lin, Jian Ye
Summary: This study investigates the interaction between nanoparticles and the pulmonary surfactant monolayer, focusing on the effects of nanoparticle elasticity on translocation across the monolayer. Molecular dynamics simulations reveal that increasing stiffness enhances the penetrability of nanoparticles, while soft nanoparticles remain trapped within the monolayer. Active cellular uptake pathways exhibit longer penetration times, greater penetration distances, and more contacts compared to passive pathways, due to ligand-receptor interactions.
Article
Chemistry, Multidisciplinary
Enrico Mastrobattista
Summary: This article introduces the author's research work and future research plans at the Department of Pharmaceutical Sciences at Utrecht University, focusing on the administration and fate control of biotherapeutics. The author's research interests include formulation of biotherapeutics into nanomedicines, intracellular delivery of nucleic acids and protein therapeutics, and targeted delivery of biotherapeutics beyond the liver. Additionally, the author provides an outlook on how current developments in drug delivery and gene therapy may lead to sustainable and cost-effective dosing regimens for biotherapeutics.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Pharmacology & Pharmacy
Agata Noelia Traverso, David Jose Fragale, Diego Luis Viale, Octavio Garate, Pablo Torres, Gaston Valverde, Alejandro Berra, Ana Vanesa Torbidoni, Juan Sebastian Yakisich, Mariano Grasselli, Martin Radrizzani
Summary: In this study, a two-step procedure for preparing biohybrid nanoparticles with a hydrophobic quantum dot core and a multilayer coating of human serum albumin is reported. These nanoparticles, decorated with proteins like human serum albumin or human transferrin, retained their fluorescent properties and showed no corona effect in the presence of serum. They were selectively taken up by lung cancer and neuroblastoma cells but not by non-cancerous cells or differentiated neurons. Moreover, digitoxin-loaded transferrin-decorated nanoparticles specifically reduced the number of lung cancer cells. Finally, the in vivo uptake of these biohybrid nanoparticles by retinal cells in mice demonstrated their excellent targeting and traceability.
Article
Pharmacology & Pharmacy
Vincent Jannin, Cindy Duysburgh, Vanessa Gonzalez, Marlies Govaert, Marine Agisson, Massimo Marzorati, Nicolas Madit
Summary: The dissolution characteristics of five capsules were evaluated using an in vitro simulation. The NGE capsules showed similar protection to the Creon capsules and demonstrated high pancrelipase activity. This is important for formulation development and first-in-human studies of pH sensitive drugs requiring small intestine targeting.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Masato Machida, Toshiki Sugimura, Shinji Kajimoto, Farsai Taemaitree, Yoshitaka Koseki, Hitoshi Kasai, Takakazu Nakabayashi
Summary: Nano-DDS, a drug delivery system using nanoparticles, is a promising tool to reduce adverse drug reactions and maximize drug efficiency. This study tracked the intracellular behavior of cholesterol-linked SN-38 (CLS) nanoparticles in cells using Raman and autofluorescence imaging. The results showed that CLS nanoparticles were taken into cells and converted into SN-38, which mainly localized in small regions in the cytoplasm. The combination of Raman and autofluorescence imaging provided insight into the intracellular behavior of prodrug nanoparticles and the cell response.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Review
Biochemistry & Molecular Biology
Snigdha Samarpita, Xiaogang Li
Summary: In recent years, the use of clinical-grade exosomes has been increasing rapidly due to their potential in advanced therapies and diagnostics for various diseases. Compared to other drug carriers, exosomes have high stability, diverse cargo loads, low immunogenicity and toxicity, making them promising in therapeutic development. This article discusses the biogenesis of exosomes, current clinical trials, the prospects and challenges of using exosomes as drug carriers, with a focus on their applications in targeting Th17 cells in diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Selvaraj Sengottiyan, Alicja Mikolajczyk, Tomasz Puzyn
Summary: The lack of knowledge about the uptake of NPs by biological cells is a significant problem for drug delivery. In order to address this problem, molecular modeling studies have been conducted to describe the mechanism of cellular uptake of drug-loaded nanoparticles. In this study, three different models were developed to predict the cellular uptake mechanism of drug-loaded nanoparticles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Joel S. Bloch, Jeffrey M. Sequeira, Ana S. Ramirez, Edward Quadros, Kaspar P. Locher
Summary: Cellular uptake of vitamin B-12 in humans is mediated by the endocytosis of the B-12 carrier protein transcobalamin, which is associated with the potential target for cancer therapy. The development of nanobodies with the ability to inhibit the growth of human cancer cells and the mapping of binding epitopes through crystal structures provide insight into the mechanisms of cellular uptake and potential therapeutic interventions.
Review
Pharmacology & Pharmacy
Bo Peng, Hui Li, Xuan-xian Peng
Summary: Existing antibacterial agents are facing the challenge of bacterial insensitivity, calling for the urgent development of novel approaches and/or antibacterials. Metabolite(s)-based adjuvants that enhance antibiotic efficacy without developing resistance are proposed as a promising strategy for combating antibiotic-resistant pathogens.
DRUG DISCOVERY TODAY
(2023)
Review
Engineering, Biomedical
Molly Klimak, Robert J. Nims, Lara Pferdehirt, Kelsey H. Collins, Natalia S. Harasymowicz, Sara J. Oswald, Lori A. Setton, Farshid Guilak
Summary: Immunoengineering has advanced new approaches for treating joint diseases such as osteoarthritis and rheumatoid arthritis, emphasizing the importance of precise targeting and controlled delivery strategies for better therapeutic outcomes. By leveraging biomaterials and genetic engineering, immunoengineering has the potential to revolutionize the understanding, treatment, and prevention of joint diseases.
ACTA BIOMATERIALIA
(2021)
Review
Nanoscience & Nanotechnology
Young Hun Chung, Derek Church, Edward C. Koellhoffer, Elizabeth Osota, Sourabh Shukla, Edward P. Rybicki, Jonathan K. Pokorski, Nicole F. Steinmetz
Summary: This article discusses the production and application of biologics, emphasizing the importance of combining plant molecular farming with advanced materials technology, especially in addressing vaccine cold chain requirements. Through the production of viruses in plants and the use of new delivery technologies, low-cost and regional fabrication of thermostable vaccines can be achieved.
NATURE REVIEWS MATERIALS
(2022)
Article
Engineering, Biomedical
Isabella C. Young, Priya Srinivasan, Roopali Shrivastava, Rima Janusziewicz, Allison Thorson, Mackenzie L. Cottrell, Rani S. Sellers, Craig Sykes, Amanda Schauer, Dawn Little, Kristen Kelley, Angela D. M. Kashuba, David Katz, Richard B. Pyles, J. Gerardo Garcia-Lerma, Kathleen L. Vincent, James Smith, S. Rahima Benhabbour
Summary: The article introduces a 3D printed multipurpose prevention technology (MPT) intravaginal ring (IVR) which can be used for HIV prevention and contraception. The MPT IVR offers a sustained release of drugs for up to 150 days in vitro and 14 days in sheep. It has been found to be safe and well tolerated in macaques, making it a potential option for young women and girls.
Article
Nanoscience & Nanotechnology
Ankan Kumar Sarkar, Santanu Shaw, Himali Arora, Pankaj Seth, Nikhil R. R. Jana
Summary: Subcellular targeting of drugs can be enhanced by designing nanocarriers for direct cell membrane translocation, bypassing endosomal/lysosomal trafficking. This approach allows rapid and extensive delivery of drugs to the cell nucleus within a few minutes, without undergoing vesicular coating and lysosomal trafficking. This nonendocytic uptake method provides a potential strategy for nanocarrier-based subcellular targeting of drugs for more effective therapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Daniel Stengel, Betuel Hilal Demirel, Patrick Knoll, Martyna Truszkowska, Flavia Laffleur, Andreas Bernkop-Schnuerch
Summary: This study evaluated the impact of polyethylene glycol (PEG) and zwitterionic surface decoration of lipid-based nanocarriers (NC) on cellular uptake. The results showed that zwitterionic lipid-based NCs exhibited better performance in terms of particle size, stability, and cellular uptake compared to PEGylated NCs. The replacement of PEG surfactants with zwitterionic surfactants may be a promising approach for intracellular drug delivery.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)