Review
Biochemistry & Molecular Biology
Naoki Shigi
Summary: Sulfur-containing biomolecules play crucial roles in cells, including enzyme cofactors, RNA composition, and regulation of gene translation efficiency. Modulating sulfur modification allows cells to adapt to different environments. Mutations in related genes can lead to diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yi Tong Vincent Aw, Azadeh Seidi, Jenni A. Hayward, Jiwon Lee, F. Victor Makota, Melanie Rug, Giel G. van Dooren
Summary: Fe-S clusters are essential for the survival of eukaryotes, with independent biosynthesis pathways occurring in different compartments of the cell. Little is known about cytosolic Fe-S cluster biosynthesis in apicomplexan parasites, but it has been found that NBP35 in Toxoplasma gondii localizes to the outer mitochondrial membrane and is critical for parasite proliferation.
MOLECULAR MICROBIOLOGY
(2021)
Article
Oncology
Fangchun Shao, Yanchun Li, Wanye Hu, Jiaqi Yu, HengYu Wu, Kejing Ying, Jun Xia, Jing Du
Summary: CISD2, a NEET protein critical for iron homeostasis and cancer proliferation, is highly expressed in NSCLC and associated with poor survival outcomes. Inhibition of CISD2 leads to mitochondrial dysfunction and activates iron starvation response, suggesting it as a potential target for antitumor therapy based on oxidative stress.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dafne Suraci, Giovanni Saudino, Veronica Nasta, Simone Ciofi-Baffoni, Lucia Banci
Summary: The study reveals the coordinated mechanism of ISCA1, ISCA2, and NFU1 in the maturation process of mitochondrial [4Fe-4S] proteins. ISCA1 plays a key role in promoting the interaction between NFU1 and ISCA2, leading to the formation of a transient ISCA1-ISCA2-NFU1 ternary complex. This mechanism ensures the safe transfer of [4Fe-4S] clusters from the assembly site on the ISCA1-ISCA2 complex to NFU1, making them available for mitochondrial apo proteins that require NFU1.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Arthavan Selvanathan, Bindu Parayil Sankaran
Summary: Iron-sulfur clusters (ISCs) are highly conserved moieties found in crucial proteins in bacteria, plants and mammals, with defects in the ISC pathway being associated with various human disease states. These disorders often involve neurological phenotypes and share common biochemical features. Therapies are primarily supportive, but improved understanding of the pathophysiology may lead to disease-modifying therapies in the future.
Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Deyamira Matuz-Mares, Martin Gonzalez-Andrade, Minerva Georgina Araiza-Villanueva, Maria Magdalena Vilchis-Landeros, Hector Vazquez-Meza
Summary: The article highlights the importance of calcium in cellular processes and discusses how cells buffer calcium by compartmentalizing it into mitochondria and the endoplasmic reticulum. It further explores the relationship between calcium homeostasis imbalance and neurodegenerative diseases, summarizing some therapeutic approaches for these diseases.
Review
Plant Sciences
Alicia Lopez-Lopez, Olivier Keech, Nicolas Rouhier
Summary: This mini-review highlights the recent progresses in our understanding of how specificity is achieved during the delivery of preformed Fe-S clusters to complex I subunits.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Yao Tian, Yuanliangzi Tian, Zhixiao Yuan, Yutian Zeng, Shuai Wang, Xiaolan Fan, Deying Yang, Mingyao Yang
Summary: This review discusses the role of iron metabolism in aging and age-related neurodegenerative diseases, as well as recent developments in using iron chelators as treatment strategies. Understanding iron metabolism is crucial for filling the knowledge gap in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Felipe A. Carvalho, Ulrich Muehlenhoff, Joseph J. Braymer, Vasilij Root, Martin Stuempfig, Carla C. Oliveira, Roland Lill
Summary: Systematic studies have shown that the Hsp90 chaperone system and Fe/S protein biogenesis or iron regulation are interconnected. DJA5 and DJA6 are two chloroplast-localized proteins that act as iron donors in plastidial Fe/S protein biogenesis. In this study, the impact of Hsp90 chaperone, yeast DJA5-DJA6 homologs, cytosolic Ydj1, and mitochondrial Mdj1 on cellular iron-related processes was investigated using Saccharomyces cerevisiae. Depletion of these crucial proteins resulted in severe phenotypes, but did not have a critical in vivo impact on Fe/S protein biogenesis or iron regulation. Unlike plant DJA5-DJA6 iron chaperones, Ydj1 and Mdj1 did not bind iron in vivo, suggesting that zinc is used for their function under normal physiological conditions.
Article
Biochemistry & Molecular Biology
Beatrice Bargagna, Lucia Banci, Francesca Camponeschi
Summary: Multiple mitochondrial dysfunctions syndrome type 2 with hyperglycinemia (MMDS2) is a severe disorder caused by biallelic mutations in the BOLA3 gene, resulting in impaired function of mitochondrial respiratory complexes and lipoic acid-dependent enzymes. The p.His96Arg mutation in BOLA3 leads to the formation of a non-functional heterocomplex with GLRX5, preventing the assembly of a [4Fe-4S] cluster on NFU1. These findings help explain the severe phenotype observed in MMDS2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Xi Jiang, Lucia Paya-Tormo, Diana Coroian, Ines Garcia-Rubio, Rocio Castellanos-Rueda, Alvaro Eseverri, Gema Lopez-Torrejon, Stefan Buren, Luis Manuel Rubio
Summary: By constructing a knowledge-based library and screening, researchers identified superior NifH variants for expression in plant mitochondria and yeast, which could potentially facilitate efficient nitrogen fixation engineering in eukaryotes.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Review
Chemistry, Inorganic & Nuclear
Jason C. Crack, Nick E. Le Brun
Summary: Iron-sulfur (FeS) clusters are protein cofactors essential for various biological functions such as electron transfer, catalysis, and gene regulation. Recent progress in using mass spectrometry has provided new insights into FeS cluster assembly and chemistry, particularly in transcriptional regulators coordinating cellular responses to changing conditions.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Ecology
Oemer K. Coskun, Aurele Vuillemin, Florence Schubotz, Frieder Klein, Susanna E. Sichel, Wolfgang Eisenreich, William D. Orsi
Summary: Thermodynamic models predict that H-2 is beneficial for seafloor microbial life, and this study found that H-2 can increase carbon assimilation by microbial communities associated with peridotite rocks. The unique geochemistry of peridotite-hosted ecosystems has selected for H-2-metabolizing, rock-associated taxa that can enhance anabolism under high H-2 concentrations. These findings suggest that the link between H-2 and carbon assimilation may be widespread in geological settings where ultramafic rocks are present.
Article
Ecology
Anna S. Weiss, Anna G. Burrichter, Abilash Chakravarthy Durai Raj, Alexandra von Strempel, Chen Meng, Karin Kleigrewe, Philipp C. Muench, Luis Roessler, Claudia Huber, Wolfgang Eisenreich, Lara M. Jochum, Stephanie Goeing, Kirsten Jung, Chiara Lincetto, Johannes Huebner, Georgios Marinos, Johannes Zimmermann, Christoph Kaleta, Alvaro Sanchez, Baerbel Stecher
Summary: This study focuses on the growth and metabolic interactions of the Oligo-Mouse-Microbiota (OMM12) synthetic bacterial community in gut microbiome research. By conducting experiments, it was found that the OMM12 interaction network is influenced by exploitative and interference competition, with Enterococcus faecalis KB1 identified as an important driver of community composition.
Correction
Ecology
Omer K. Coskun, Aurele Vuillemin, Florence Schubotz, Frieder Klein, Susanna E. Sichel, Wolfgang Eisenreich, William D. Orsi
Article
Biochemistry & Molecular Biology
Adrian Drazic, Evy Timmerman, Ulrike Kajan, Michael Marie, Sylvia Varland, Francis Impens, Kris Gevaert, Thomas Arnesen
Summary: Actin is a crucial protein in the cytoskeleton of eukaryotic cells, and its dynamics are regulated by various actin-binding proteins and post-translational modifications. This study provides detailed insights into the N-terminal processing of actin and challenges the previously claimed N-terminal arginylation of beta-actin.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Fatma Acheuk, Shereen Basiouni, Awad A. Shehata, Katie Dick, Haifa Hajri, Salma Lasram, Mete Yilmaz, Mevlut Emekci, George Tsiamis, Marina Spona-Friedl, Helen May-Simera, Wolfgang Eisenreich, Spyridon Ntougias
Summary: Safe alternatives to synthetic pesticides are urgently needed for human and environmental health. Botanical pesticides, which are effective and easily biodegradable, have the potential to be a solution. However, the development of commercial biopesticides from plant species is limited, and there are several constraints and challenges that need to be addressed.
Review
Microbiology
Elias Asimakis, Awad A. Shehata, Wolfgang Eisenreich, Fatma Acheuk, Salma Lasram, Shereen Basiouni, Mevlut Emekci, Spyridon Ntougias, Goekce Taner, Helen May-Simera, Mete Yilmaz, George Tsiamis
Summary: This review discusses the potential of algae as biopesticides, focusing on macroalgae and microalgae (including cyanobacteria) and their extracts or purified compounds. The effectiveness and mechanisms of action of these compounds in areas such as antibacterial, antiviral, antifungal, nematocides, insecticides, herbicides, and plant growth stimulation are thoroughly discussed.
Review
Microbiology
Awad A. Shehata, Sakine Yalcin, Juan D. Latorre, Shereen Basiouni, Youssef A. Attia, Amr Abd El-Wahab, Christian Visscher, Hesham R. El-Seedi, Claudia Huber, Hafez M. Hafez, Wolfgang Eisenreich, Guillermo Tellez-Isaias
Summary: The gut microbiota plays a significant role in host metabolism, physiology, nutrition, and immune function. The interaction between the gut microbiota and their host animals is mutually beneficial and dynamic. Manipulating the gut microbiota through dietary supplementation with prebiotics, probiotics, and phytogenic substances can control metabolic diseases and improve animal health and performance. These compounds indirectly influence the gut microbiota quality and enhance the integrity of the intestinal epithelial barrier.
Article
Food Science & Technology
Sandra Radziej, Julia Scherb-Forster, Claus Schlicht, Wolfgang Eisenreich
Summary: An improved protocol for fast identification of food thickeners using H-1-NMR spectroscopy was reported. The method involves centrifugation, fractionation, and NMR analysis, allowing unequivocal identification of high and low molecular weight thickeners.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Anja Devenie Riegel, Hidehiko Wakabayashi, Motoko Wakabayashi, Marketa Rynesova, Viktoriia Dudko, Wolfgang Eisenreich, Karl-Heinz Engel
Summary: Through studying the stereoisomers of two 1,3-oxathianes, it was found that the positions and spatial orientations of substituents have a significant impact on their sensory properties. Different stereoisomers have different odor thresholds and odor qualities.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Thomas Steiner, Marie Zachary, Susanne Bauer, Martin J. Mueller, Markus Krischke, Sandra Radziej, Maximilian Klepsch, Bruno Huettel, Wolfgang Eisenreich, Thomas Rudel, Dagmar Beier
Summary: Through transcriptome and metabolome analysis, we have demonstrated the significant role of the sibling sRNAs NgncR_162/163 in the regulation of metabolic pathways, particularly in the biosynthesis of amino acids and nucleotides.
Article
Chemistry, Multidisciplinary
Ran Li, Wolfgang Eisenreich, Lei Lei, Johann Plan
Summary: This study investigated the role of polycarboxylate superplasticizers in a low carbon alkali-activated slag (AAS) system. Two series of PCEs with different anionicities were synthesized and characterized. The results showed that the synthesized HPEG PCEs exhibited superior dispersing performance over APEG PCEs, especially at high anionicity. The dispersing power of the PCEs increased with their anionicity as a result of stronger adsorption on slag. 13C NMR spectroscopy was used to identify specific structural motifs in the PCE copolymers.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Cell Biology
Katharina Papsdorf, Jason W. Miklas, Amir Hosseini, Matias Cabruja, Christopher S. Morrow, Marzia Savini, Yong Yu, Carlos G. Silva-Garcia, Nicole R. Haseley, Luke Meraz Murphy, Pallas Yao, Elisa de Launoit, Scott J. Dixon, Michael P. Snyder, Meng C. Wang, William B. Mair, Anne Brunet
Summary: A study found that dietary mono-unsaturated fatty acids (MUFAs) are linked to longevity in several species. The mechanisms by which MUFAs extend lifespan involve an organelle network involving lipid droplets and peroxisomes. MUFAs increase the number of lipid droplets and peroxisomes, and this regulation of organelle abundance is critical for MUFA-induced longevity.
NATURE CELL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Philippe Diederich, Alexander Ruf, Thomas Geisberger, Leopold Weidner, Christian Seitz, Wolfgang Eisenreich, Claudia Huber, Philippe Schmitt-Kopplin
Summary: Chemical complexity is crucial for the origin of life and biological evolution. The chemical evolution of a complex prebiotic mixture containing acetylene, carbon monoxide, and nickel sulfide has been analyzed using mass spectrometry. Isotopic labeling reveals the presence of diverse compounds within the reaction mixture. Different degrees of labeling enable robust characterization of functional groups. The study also reveals a characteristic addition pattern and a high diversity of sulfur-containing compounds. The temporal analysis provides insights into the role of sulfur in the origin of life.
COMMUNICATIONS CHEMISTRY
(2023)
Review
Veterinary Sciences
Shereen Basiouni, Guillermo Tellez-Isaias, Juan D. Latorre, Brittany D. Graham, Victor M. Petrone-Garcia, Hesham R. El-Seedi, Sakine Yalcin, Amr Abd El-Wahab, Christian Visscher, Helen L. May-Simera, Claudia Huber, Wolfgang Eisenreich, Awad A. Shehata
Summary: Chronic stress and inflammation have detrimental impacts on the health and performance of animals, including poultry. This review discusses the mechanistic pathways of oxidative stress and inflammation associated with the main stressors in poultry, such as heat stress, dysbiosis, leaky gut syndrome, and mycotoxins. The use of phytogenic bioactive substances to mitigate oxidative stress and inflammation in poultry is also explored.
VETERINARY SCIENCES
(2023)
Article
Biology
Lydia Steffens, Eugenio Pettinato, Thomas M. Steiner, Wolfgang Eisenreich, Ivan A. Berg
Summary: Different pathways for autotrophic CO2 fixation can be recognized by the presence of genes for their specific key enzymes. However, some pathways like the reverse oxidative tricarboxylic acid (roTCA) cycle lack unique enzymes, which makes them difficult to analyze using bioinformatics. This article presents a detailed description of a protocol used to identify the roTCA cycle in members of Desulfurellaceae, including anaerobic cultivation, enzyme activity measurements, and stable isotope experiments. These methods can be used to evaluate the functioning of the roTCA cycle in any organism under study.