Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes

Generation of viable PDX1 gene‐edited founder pigs as providers of nonmosaics

(2020) Fuminori Tanihara et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes

(2019) Maki Hirata et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Production of non-mosaic genome edited porcine embryos by injection of CRISPR/Cas9 into germinal vesicle oocytes

(2019) Xiaohu Su et al.   Journal of Genetics and Genomics

Genome-Wide Off-Target Analysis in CRISPR-Cas9 Modified Mice and Their Offspring

(2019) Yan Dong et al.   G3-Genes Genomes Genetics

A glance at genome editing with CRISPR–Cas9 technology

(2019) Antara Barman et al.   CURRENT GENETICS

CRISPR/Cas9-mediated genome editing in nonhuman primates

(2019) Yu Kang et al.   Disease Models & Mechanisms

Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2

(2019) Konrad Fischer et al.   XENOTRANSPLANTATION

Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH

(2018) Runjie Zhang et al.   Acta Biomaterialia

Generation of GTKO Diannan Miniature Pig Expressing Human Complementary Regulator Proteins hCD55 and hCD59 via T2A Peptide-Based Bicistronic Vectors and SCNT

(2018) Fengjuan Liu et al.   MOLECULAR BIOTECHNOLOGY

Xenotransplantation: back to the future?

(2018) Raphael P. H. Meier et al.   TRANSPLANT INTERNATIONAL

B4GALNT2 and xenotransplantation: A newly appreciated xenogeneic antigen

(2018) Guerard Byrne et al.   XENOTRANSPLANTATION

GalT-KO pig lungs are highly susceptible to acute vascular rejection in baboons, which may be mitigated by transgenic expression of hCD47 on porcine blood vessels

(2018) Hironosuke Watanabe et al.   XENOTRANSPLANTATION

Strategies for controlling CRISPR/Cas9 off-target effects and biological variations in mammalian genome editing experiments

(2018) Michelle L. Kimberland et al.   JOURNAL OF BIOTECHNOLOGY

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   PLoS One

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   ANIMAL SCIENCE JOURNAL

Generation by somatic cell nuclear transfer of GGTA1 knockout pigs expressing soluble human TNFRI-Fc and human HO-1

(2018) Geon A Kim et al.   TRANSGENIC RESEARCH

Efficient mutagenesis by CRISPR/Cas system during meiotic maturation of porcine oocytes

(2017) Asuka ONUMA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning

(2017) Hanchao GAO et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos

(2017) K Nishio et al.   REPRODUCTION IN DOMESTIC ANIMALS

Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes

(2017) T-V Nguyen et al.   REPRODUCTION IN DOMESTIC ANIMALS

Humoral Reactivity of Renal Transplant-Waitlisted Patients to Cells From GGTA1/CMAH/B4GalNT2, and SLA Class I Knockout Pigs

(2017) Gregory R. Martens et al.   TRANSPLANTATION

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

(2017) Christine Burkard et al.   PLoS Pathogens

Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model

(2017) Junfang Zhang et al.   Scientific Reports

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Analyzing CRISPR genome-editing experiments with CRISPResso

(2016) Luca Pinello et al.   NATURE BIOTECHNOLOGY

Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer

(2016) Wenmin Cheng et al.   Reproductive Biology and Endocrinology

Gene targeting, genome editing: from Dolly to editors

(2016) Wenfang Tan et al.   TRANSGENIC RESEARCH

Silencing the porcine iGb3s gene does not affect Galα3Gal levels or measures of anticipated pig-to-human and pig-to-primate acute rejection

(2016) James R. Butler et al.   XENOTRANSPLANTATION

Efficient production of biallelicGGTA1knockout pigsbycytoplasmic microinjection of CRISPR/Cas9 into zygotes

(2016) Bjoern Petersen et al.   XENOTRANSPLANTATION

Human antibody reactivity against xenogeneicN-glycolylneuraminic acid and galactose-α-1,3-galactose antigen

(2016) Sunghoon Hurh et al.   XENOTRANSPLANTATION

Somatic cell reprogramming-free generation of genetically modified pigs

(2016) F. Tanihara et al.   Science Advances

Characteristics of α-Gal epitope, anti-Gal antibody, α1,3 galactosyltransferase and its clinical exploitation (Review)

(2015) GUOLI HUAI et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes

(2015) Jose L. Estrada et al.   XENOTRANSPLANTATION

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

(2014) Tang Hai et al.   CELL RESEARCH

The current state of xenotransplantation

(2014) J. Zeyland et al.   JOURNAL OF APPLIED GENETICS

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Production and characterization of soluble human TNFRI-Fc and human HO-1(HMOX1) transgenic pigs by using the F2A peptide

(2014) Sol Ji Park et al.   TRANSGENIC RESEARCH

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

(2013) Mayuko Kurome et al.   BMC BIOTECHNOLOGY

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

Genetically Modified Pig Models for Human Diseases

(2013) Nana Fan et al.   Journal of Genetics and Genomics

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

(2013) Jige Xin et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

(2011) J. Hauschild et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of Soluble Human Tumor Necrosis Factor-α Receptor 1-Fc Transgenic Pig

(2011) Bumrae Cho et al.   TRANSPLANTATION

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Genetic modification of pigs as organ donors for xenotransplantation

(2009) Nikolai Klymiuk et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT