Article
Chemistry, Multidisciplinary
Natalie Landon-Brace, Jose L. Cadavid, Simon Latour, Ileana L. Co, Darren Rodenhizer, Nancy T. Li, Nila C. Wu, Eryn Bugbee, Aleks Chebotarev, Ji Zhang, Bradly G. Wouters, Alison P. McGuigan
Summary: TRACER2 serves as a novel platform to dissect the effects of microenvironmental parameters on tumor cell function, especially in the study of pancreatic cancer PDOs.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Physical
Alexei D. Filippov, Joris Sprakel, Marleen Kamperman
Summary: The research discusses the control of viscous and elastic behavior in the application of complex coacervates in areas such as coatings and surgical glues, and proposes a model with customizable rheological characteristics. It is found that when the contribution of metal-ligand bonds is dominant at long times, the relaxation of the overall complex is significantly slower.
Article
Oncology
Xuan Zou, Xuan Lin, He Cheng, Yusheng Chen, Ruijie Wang, Mingjian Ma, Yu Liu, Zhengjie Dai, Yesboli Tasiheng, Yu Yan, Qinqin Hou, Fei Ding, Huan Chen, Xianjun Yu, Xu Wang, Chen Liu
Summary: This study systematically revealed the cellular properties and prognostic values of intratumoral TLSs in pancreatic ductal adenocarcinoma (PDAC) and described the potential impact of neoadjuvant treatment (NAT) on TLS development and function.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Engineering, Biomedical
Minh-Chau N. Le, Kailei Xu, Zi Wang, Sean Beverung, Robert L. Steward, Stephanie J. Florczyk
Summary: This study investigated the response of BCa cells to CA scaffold stiffness by preparing CA scaffolds of different concentrations. It was found that 231 cells responded to the stiffness of the CA scaffolds and formed clusters of different sizes. Cell migration speed also varied on different surfaces, suggesting a non-integrin-based mechanism may explain the observed mechanotransduction response.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Liaoran Niu, Qi Wang, Fan Feng, Wanli Yang, Zhenyu Xie, Gaozan Zheng, Wei Zhou, Lili Duan, Kunli Du, Yiding Li, Ye Tian, Junfeng Chen, Qibin Xie, Aqiang Fan, Hanjun Dan, Jinqiang Liu, Daiming Fan, Liu Hong, Jian Zhang, Jianyong Zheng
Summary: This review provides a comprehensive summary of the interaction between cancer cells and macrophages in the tumor microenvironment, and discusses the role of small extracellular vesicles (sEVs) in this process. It also explores the various effects of macrophage-secreted sEVs on tumor malignant transformation, and addresses the therapeutic advancements and challenges associated with these vesicles.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Immunology
Xue Liu, Chenyang Xu, Tianwei Guo, Shenghua Zhan, Qiuying Quan, Mengsi Li, Ziyi Wang, Xueguang Zhang, Lingchuan Guo, Lei Cao
Summary: CD155 expression is positively correlated with tumor microenvironment cell infiltration and gastric adenocarcinoma progression. It is associated with aggressive clinicopathological features and immune-related factors. CD155 may play a pivotal role in gastric adenocarcinoma development and become a novel target for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Malcolm Holterhus, Bianca Altvater, Sareetha Kailayangiri, Claudia Rossig
Summary: Cellular immunotherapy has shown limited efficacy in solid cancers of childhood and adolescence due to the presence of immunosuppressive cells in the tumor microenvironment. Strategies to target these cells, such as myeloid cell populations, with pharmacological agents are discussed, as well as novel approaches aiming to harness these cells to enhance antitumor immune responses.
Article
Immunology
Jiaxiong Tan, Chaoyu Wang, Yan Jin, Yuren Xia, Baocheng Gong, Qiang Zhao
Summary: The study found that a signature constructed with six genes can better predict the prognosis of neuroblastoma and evaluate the immunosuppressed and aging tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Manuela Sollazzo, Monica De Luise, Silvia Lemma, Licia Bressi, Maria Iorio, Stefano Miglietta, Sara Milioni, Ivana Kurelac, Luisa Iommarini, Giuseppe Gasparre, Anna Maria Porcelli
Summary: Mitochondria play crucial roles in cellular bioenergetics and biosynthetic processes, producing signals that regulate molecular networks governing proliferation and cell death. Even in pathological contexts like tumorigenesis, mitochondrial dysfunction can confer flexibility favoring cancer cell survival. Despite initial growth delays, cancer cells with respiratory complex I impairment may adapt to resume proliferation through molecular mechanisms.
Article
Oncology
Michael F. Kaminski, Laura Bendzick, Rachel Hopps, Marissa Kauffman, Behiye Kodal, Yvette Soignier, Peter Hinderlie, Joshua T. Walker, Todd R. Lenvik, Melissa A. Geller, Jeffrey S. Miller, Martin Felices
Summary: This study generated a Tri-specific Killer Engager (TriKE) against TEM8, which selectively activates NK cells to kill TEM8-expressing tumor cells and stromal cells, providing a novel anti-tumor, anti-stroma, and anti-angiogenic cancer therapy for patients with solid tumors.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Biochemistry & Molecular Biology
Michela Cortesi, Michele Zanoni, Francesca Pirini, Maria Maddalena Tumedei, Sara Ravaioli, Ilario Giovanni Rapposelli, Giovanni Luca Frassineti, Sara Bravaccini
Summary: Pancreatic ductal adenocarcinoma (PDAC) has a poor prognosis due to immune suppression and the tumor microenvironment. This review explores the interplay between senescent and non-senescent cell types and their impact on PDAC progression. The non-tumoral cells in the tumor microenvironment influence key aspects of tumor growth, metabolism, cell death, and treatment resistance. Understanding these interactions is crucial for PDAC treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Sasitorn Yenyuwadee, Konstantinos Aliazis, Qi Wang, Anthos Christofides, Rushil Shah, Nikolaos Patsoukis, Vassiliki A. Boussiotis
Summary: In the past decade, antibody-based and cell-based immunotherapies have revolutionized cancer therapeutics by modulating immune responses against tumors. However, the full potential of immunotherapy has not been reached due to incomplete knowledge of the cellular and molecular mechanisms involved in successful anti-tumor immunity and immune escape.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Itayetzi Reyes-Avendano, Edilburga Reyes-Jimenez, Karina Gonzalez-Garcia, Dulce Carolina Perez-Figueroa, Rafael Baltierrez-Hoyos, Gabriela Tapia-Pastrana, Xariss Miryam Sanchez-Chino, Saul Villa-Trevino, Jaime Arellanes-Robledo, Veronica Rocio Vasquez-Garzon
Summary: Quercetin has been found to reduce tumor microenvironment components and the expression of ABCC3, a marker of HCC progression and multi-drug resistance, in the early stages of hepatocellular carcinoma.
Review
Cell Biology
Xingyu Chen, Yongyun Li, Tengteng Yao, Renbing Jia
Summary: Zebrafish are widely used in cancer research due to their low cost, quick establishment, and small sample size required. The zebrafish patient-derived xenograft (zPDX) models show promising evidence for short-term clinical treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biology
Aleksandra Bozyk, Kamila Wojas-Krawczyk, Pawel Krawczyk, Janusz Milanowski
Summary: Scientists worldwide have been studying the biology of cancer, revealing that its development is not only caused by genetic mutations but also by complex interactions between molecules and cells. Understanding the tumor microenvironment is crucial in developing effective anti-cancer treatments and combating immune system evasion by cancer cells.
Article
Engineering, Biomedical
Amir Rouhollahi, Olusegun Ilegbusi, Stephen Florczyk, Kailei Xu, Hassan Foroosh
ANNALS OF BIOMEDICAL ENGINEERING
(2020)
Article
Engineering, Biomedical
Kailei Xu, Kavya Ganapathy, Thomas Andl, Zi Wang, John A. Copland, Ratna Chakrabarti, Stephen J. Florczyk
Article
Engineering, Biomedical
Minh-Chau N. Le, Kailei Xu, Zi Wang, Sean Beverung, Robert L. Steward, Stephanie J. Florczyk
Summary: This study investigated the response of BCa cells to CA scaffold stiffness by preparing CA scaffolds of different concentrations. It was found that 231 cells responded to the stiffness of the CA scaffolds and formed clusters of different sizes. Cell migration speed also varied on different surfaces, suggesting a non-integrin-based mechanism may explain the observed mechanotransduction response.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biotechnology & Applied Microbiology
Kailei Xu, Xixia Liu, Xiaokeng Li, Jun Yin, Peng Wei, Jin Qian, Jie Sun
Summary: The study demonstrated that electromechanical stimulation affects the proliferation and axon outgrowth of nerve cells, with mechanical stretching reducing these effects. Cellular axons prefer to align perpendicularly to the stretch direction, showing a dependence on stretching direction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Clinical Neurology
Kai Ye, Andong He, Miaoben Wu, Xiaodong Qiu, Zhiwu Chen, Jun Yin, Qinghua Song, Yi Huang, Kailei Xu, Yuye Huang, Peng Wei
Summary: This study explores the potential of decellularized kidney and decellularized peripheral nerve as replacements for nerve repair and regeneration. The results show that while the cell behaviors are similar, the arrangement of cells on the decellularized peripheral nerve scaffold is more regular. Furthermore, the expression of axon growth-associated genes and proteins is significantly upregulated in the decellularized kidney group, which also contains higher levels of growth factors. This suggests that decellularized kidney may have a potential application in promoting nerve repair and regeneration.
FRONTIERS IN NEUROLOGY
(2022)
Review
Cell & Tissue Engineering
Kailei Xu, Enxing Yu, Miaoben Wu, Peng Wei, Jun Yin
Summary: Alopecia is a common medical condition that affects a majority of people worldwide, leading to significant impacts on quality of life and self-esteem. Current treatments for alopecia have side effects, prompting the need to explore new hair-follicle regeneration approaches. Tissue engineering shows promise in regenerating hair follicles by simulating cellular interactions, and biomaterials play a crucial role in this process.
REGENERATIVE THERAPY
(2022)
Article
Engineering, Biomedical
Kailei Xu, Ying Han, Yuye Huang, Peng Wei, Jun Yin, Junhui Jiang
Summary: Urologic diseases are a common health problem worldwide and major causes of death. Organ transplantation and tissue engineering techniques offer potential solutions for organ donor shortage. This review discusses the applications of 3D bioprinting and commonly used biomaterials in urologic diseases, showing the potential of this technology in fabricating functional organs and disease models.
MATERIALS TODAY BIO
(2022)
Article
Engineering, Biomedical
Kailei Xu, Yuye Huang, Miaoben Wu, Jun Yin, Peng Wei
Summary: Prostate cancer is a deadly cancer in men worldwide and the tumor microenvironment, consisting of various cells and extracellular matrix, plays a crucial role in its development. Hyaluronic acid and cancer-associated fibroblasts are major components in the tumor microenvironment and are associated with prostate cancer progression and metastasis, although the underlying mechanism is not fully understood due to the lack of biomimetic extracellular matrix components and coculture models.
Article
Materials Science, Biomaterials
Miaoben Wu, Enxing Yu, Kai Ye, Yuye Huang, Tiantian Ren, Mingjun Guo, Jun Yin, Kailei Xu, Peng Wei
Summary: Melanoma is a highly malignant tumor originating from melanocytes. Fibroblasts in the dermis promote melanoma metastasis, but the molecular mechanism underlying their interaction is not well understood. Gelatin methacryloyl (GelMA) was used to construct a co-culture model for melanoma cells and fibroblasts, revealing the possible mechanisms of fibroblast-melanoma interaction and suggesting potential for screening chemotherapies.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Manufacturing
Yang Li, Qijiang Mao, Kailei Xu, Huayong Yang, Yong Huang, Jun Yin
Summary: In this study, a dynamic support bath assisted vat photopolymerization printing method using yield stress fluid (YSF) was proposed. The YSF bioink can provide dynamic support to the printing structures by solidifying near the curing region and filling up the vacuum, enabling continuous forming during the rising of the moving platform. This method successfully achieved vat photopolymerization printing of extremely soft cell-laden bioinks into 3D complex constructs with excellent biological functionality.
ADDITIVE MANUFACTURING
(2023)
Article
Engineering, Biomedical
Enxing Yu, Zhiwu Chen, Yuye Huang, Yibing Wu, Zonghuan Wang, Fangfang Wang, Miaoben Wu, Kailei Xu, Wei Peng
Summary: Peripheral nerve injury (PNI) is a common and severe clinical disease with poor prognosis. Autologous nerve grafting is limited in availability and size, leading to the development of artificial nerve conduits. In this study, a novel composite neural conduit containing decellularized tissues and a grooved PLGA conduit was found to significantly promote nerve regeneration. This artificial nerve conduit could potentially be used in clinical practice.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Article
Polymer Science
Yuye Huang, Kailei Xu, Jingyi Liu, Guangli Dai, Jun Yin, Peng Wei
Summary: This study investigated the co-culture of Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on a 50% degree of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. PC-12 cells showed enhanced cell proliferation and axonal extension in the presence of neurotrophic factors secreted by SCs. A 5% GelMA scaffold was found to better support SCs proliferation, viability, and myelination compared to a 10% GelMA scaffold.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.