Article
Multidisciplinary Sciences
Jingjing Cao, Yuheng Lin, Tianpeng Zhou, Wei Wang, Quanxing Zhang, Bingcai Pan, Wei Jiang
Summary: This study presents a method for catalytic depolymerization of polyethylene terephthalate (PET) into bis(2-hydroxyethyl) terephthalate (BHET) using oxygen molecules (O-2) assisted by defect-rich ZnO. In air, the PET conversion rate, BHET yield, and space-time yield are 3.5, 10.6, and 10.6 times higher than those in nitrogen, respectively. The (100) facet of defect-rich ZnO nanosheets promotes the formation of reactive oxygen species (O-star(2)-) and Zn defects, facilitating the breakage of the ester bond in PET and completing the depolymerization process. This approach demonstrates a sustainable route for PET depolymerization through molecule-assisted defect engineering.
Article
Engineering, Chemical
Zaiwei Man, Huihui Cui, Jin Li, Zhiqiang Cai, Jing Guo
Summary: In this study, the overexpression of homologous TreS significantly improved trehalose production by simplifying the process. Using permeabilized cells as biocatalysts, trehalose synthesis could be achieved efficiently in a short period of time.
Article
Physiology
Hong-Jia Yang, Meng-Yao Cui, Xiao-Hui Zhao, Chun-Yu Zhang, Yu-Shuo Hu, Dong Fan
Summary: In this study, the Trehalose-6-phosphate synthase (TPS) gene in Mythimna separata (MsTPS) was cloned and its expression patterns in different developmental stages and tissues were analyzed. The inhibition of MsTPS expression resulted in decreased trehalose content and changed expression of chitin synthase genes. It also had negative effects on M. separata larvae, including decreased weight, feeding capacity, and increased mortality and malformation rates.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Srisakul Trakarnpaiboon, Benjarat Bunterngsook, Rungtiva Wansuksriand, Verawat Champreda
Summary: Pseudomonas monteilii TBRC 1196 has been identified as a potent strain for trehalose synthase production, which exhibits high residual activity under a broad pH range. In the lab-scale bioreactor, the purified recombinant trehalose synthase showed efficient trehalose production with low yield of glucose as a byproduct.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yi Shuang Wang, Wan Zheng, Nan Jiang, Yun Xia Jin, Zi Kang Meng, Meng Xin Sun, Yu Liang Zong, Tong Xu, Jiapeng Zhu, Ren Xiang Tan
Summary: This study discovered a new class of enzymes called ChaP that can catalyze the deconstruction of intradiol-like o-quinones and release CO2. Experimental evidence showed that by mutating and replacing specific residues, the enzyme's reaction trajectory and regioselectivity can be altered. The results of this study highlight the catalytic versatility and enzymatic plasticity of VOC enzymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Microbiology
Neera Agarwal, Sudhir P. Singh
Summary: A novel putative trehalose synthase gene (treM) with higher stability and dual transglycosylation activities of trehalose and trehalulose biosynthesis was identified. This enzyme can catalyze the transformation of maltose to trehalose and sucrose to trehalulose in a wide pH and temperature range, showing great potential for producing high-value rare sugars. The study highlights thermal aquatic habitats as a promising genetic resource for biocatalysts with applications in biological research and the food industry.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Eun Gyu Jegal, Shila Maskey, Eun Jin Cho, Yoon Gyo Lee, Dinh-Truong Nguyen, Hyeun-Jong Bae
Summary: This study developed an integrated approach of biocatalytic conversion coupled with purification using selective enzyme for continuous trehalose production. The immobilized trehalose synthase derived from Pseudomonas stutzeri showed significantly higher trehalose production yield compared to the other two enzymes after 24 hours. A continuous bioconversion system using the immobilized enzyme in a fixed-bed bioreactor achieved a high yield and productivity.
PROCESS BIOCHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Luhua Zheng, Ruiqi Zhou, Bo Jiang, Jingjing Chen, Miaomiao Hu, Tao Zhang
Summary: In this study, whole-cell catalysis was used to achieve the biotransformation of β-cyclodextrin to nonreducing maltoheptaose (N-G7) using coexpressed cyclomaltodextrinase and maltooligosyltrehalose synthase. Deletion of the lpxM gene allowed β-cyclodextrin to enter the cell, resulting in improved synthesis efficiency of N-G7. The results demonstrate that whole-cell catalysis is a promising approach for N-G7 synthesis, offering an eco-friendly and energy-efficient biotransformation system.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Srisakul Trakarnpaiboon, Benjarat Bunterngsook, Hataikarn Lekakarn, Daran Prongjit, Verawat Champreda
Summary: In this study, a cold-active trehalose synthase (PaTreS) was characterized and its properties were investigated. The results showed that the enzyme maintained good activity at lower temperatures and had stability over a broad pH range. Conversion of high-concentration maltose syrup using PaTreS yielded high levels of trehalose with low generation of byproducts.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Biotechnology & Applied Microbiology
Meijie Li, Rumeng Yang, Jing Guo, Min Liu, Jianming Yang
Summary: This study successfully improved the thermostability of isoprene synthase through directed evolution. A tripartite protein folding system was used for high-throughput screening, resulting in the identification of three variants with higher stability. In vitro stability tests confirmed the increased stability of the variants. One of the variants also showed improved isoprene production.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qiuya Gu, Yu Lu, Jianli Zhou, Wenhua Yang, Ke Wang, Xiaobo Liu, Xiaobin Yu
Summary: Combinational mutagenesis was used to improve the catalytic efficiency and adaptability of alginate lyase from Pseudoalteromonas sp. Alg6B, which showed significant potential for the production of AOS from marine alginate.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Juan Octavio Valle-Rodriguez, Verena Siewers, Jens Nielsen, Shuobo Shi
Summary: A random mutagenesis approach was used to enhance the catalytic efficiency of a wax ester synthase (WS) from Marinobacter hydrocarbonoclasticus, resulting in improved selectivity towards ethanol and shorter alcohols. This work also introduced a new high-throughput selection system for isolating WSs with desired selectivity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
So-Ra Han, Byeollee Kim, Jong Hwa Jang, Hyun Park, Tae-Jin Oh
Summary: This study compared the genomes of 16 strains within the Arthrobacter group to understand the genetic patterns and survival mechanisms of cold-adapted bacteria in extreme environments. The insight provided into glycogen metabolism and cold adaptation mechanisms in Arthrobacter species may promote further research and application in low-temperature biotechnology.
Article
Immunology
Zhaohai Wen, Muhammad Tahir Aleem, Kalibixiati Aimulajiang, Cheng Chen, Meng Liang, Xiaokai Song, Lixin Xu, Xiangrui Li, Ruofeng Yan
Summary: In this study, the Hc-TPS gene of Haemonchus contortus was cloned and its expression pattern and distribution were analyzed. The recombinant rHc-TPS protein was found to inhibit PBMC proliferation and NO secretion, while activating the IL-10/STAT3/SOCS3 axis and suppressing INF-gamma, IL-4, IL-9, and IL-2 pathways. These findings provide insights into the immune evasion mechanism of parasites and may help in the discovery of new drug targets.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Madina Rasulova, Aleksandra Zecic, Jose Manuel Monje Moreno, Lieselot Vandemeulebroucke, Ineke Dhondt, Bart P. Braeckman
Summary: The C. elegans insulin/IGF-1 signaling mutant daf-2 shows a dauer metabolic signature with increased lipid and carbohydrate accumulation, which is believed to contribute to longevity and stress resistance. The upregulation of trehalose in daf-2 mutants has been linked to proteome stabilization and protection against various stresses. However, the study demonstrates that trehalose accumulation mainly enhances osmotolerance, and partly thermotolerance, rather than impacting longevity in daf-2 mutants, suggesting that specific stress resistance phenotypes can be separated from longevity.
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)