Article
Food Science & Technology
Jinming Dai, Mei Bai, Changzhu Li, Wai San Cheang, Haiying Cui, Lin Lin
Summary: This study aimed to investigate the impact of citral concentration on its antibacterial effect against Staphylococcus aureus. The results showed that citral at a concentration of 0.5 mg/mL significantly disturbed the cell membrane fluidity and permeability, leading to cell death. However, at a concentration of 0.25 mg/mL, the membrane damage was reduced and a complex interaction between citral and the membrane components was observed. Overall, this study provides valuable insights into the antibacterial mechanisms of natural compounds and expands the potential applications of citral as a natural antimicrobial.
Article
Food Science & Technology
Han Du, Tao Ping, Wei Wu, Qingli Yang
Summary: This study prepared a simple and sensitive DNA tetrahedral fluorescence signal amplifier for sandwich detection of Staphylococcus aureus. The amplifier improved the fluorescence detection sensitivity and selectivity of S. aureus, and it may also be applicable to the detection of other food pathogens or environmental microorganisms in the future.
Article
Chemistry, Analytical
Jiandong Liu, Bin Liu, Juan Liu, Xing-Dao He, Jinhui Yuan, Zabih Ghassemlooy, Hamdi Torun, Yong-Qing Fu, Xuewu Dai, Wai Pang Ng, Richard Binns, Qiang Wu
Summary: A high quality factor (Q-factor) optical fiber interferometer based biosensing platform, combining single mode-core-only-single mode fiber (SCS) and multimode interference effect, is developed for ultra-high sensitivity biosensing. This biosensor is capable of effectively detecting concentrations of Staphylococcus aureus from 10 to 105 CFU/mL, with good sensitivity, stability, reproducibility and specificity, showing great potentials in diagnostics.
Article
Food Science & Technology
A. Arun Prince Milton, Kasanchi M. Momin, Sandeep Ghatak, Shiny C. Thomas, G. Bhuvana Priya, M. Angappan, Samir Das, R. K. Sanjukta, K. Puro, I Shakuntala, A. Sen, B. K. Kandpal
Summary: A polymerase spiral reaction (PSR) assay was developed for rapid and sensitive detection of Staphylococcus aureus in pork samples. The assay demonstrated higher sensitivity and specificity compared to traditional PCR methods, offering a cost-effective and user-friendly option for the detection of S. aureus in less-advanced laboratories.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Spectroscopy
Qin Ouyang, Yongcun Yang, Shujat Ali, Li Wang, Huanhuan Li, Quansheng Chen
Summary: The study developed a rapid, sensitive, and specific aptasensor based on FRET for detecting Staphylococcus aureus in food. Under optimized conditions, there was a significant linear correlation between fluorescence intensity and S. aureus concentration, demonstrating high detection sensitivity and accuracy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Alaa Eldin M. A. Morshdy, Karima M. E. Abdallah, Heba E. Abdallah, Fahad D. Algahtani, Mohamed Tharwat Elabbasy, Suleman Atique, Khursheed Ahmad, Mohammad A. A. Al-Najjar, Hossam M. Abdallah, Abdallah Fikry A. Mahmoud
Summary: This study found a high prevalence of Staphylococcus aureus in chicken meat samples from Egyptian markets, with a majority of the isolates being multidrug-resistant. The study also demonstrated the potential of certain natural phenolic compounds, particularly hydroquinone, as alternative antibacterial agents against these drug-resistant bacteria.
Article
Immunology
Richard A. Proctor
Summary: The concept of commensalism refers to a relationship where different species coexist, benefiting one side. There is ongoing debate about whether Staphylococcus aureus should still be considered a commensal, as it can cause serious infections. It is important to focus on how to approach this microorganism effectively.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Wei Wang, Michelle Baker, Yue Hu, Jin Xu, Dajin Yang, Alexandre Maciel-Guerra, Ning Xue, Hui Li, Shaofei Yan, Menghan Li, Yao Bai, Yinping Dong, Zixin Peng, Jinjing Ma, Fengqin Li, Tania Dottorini
Summary: The research provides a comprehensive analysis of Staphylococcus aureus in China, highlighting the challenges of antimicrobial resistance and the diversity of MRSA lineages. The study reveals a distinct evolutionary path between Chinese and European MRSA strains and the importance of machine learning in identifying resistance determinants within specific clonal complexes.
Review
Chemistry, Multidisciplinary
Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, Na Li, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, Chuanlai Xu, Yang Yang, Bi-Feng Yuan, Quan Yuan, Chao Zhang, Zhi Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan, Chunhai Fan
Summary: Nucleic acids are natural biopolymers that play central roles in storing, encoding, transmitting and expressing genetic information. The field of nucleic acids analysis has rapidly advanced with technological breakthroughs, leading to the development of new analysis methods. However, there are also challenges and further research needed in this area.
SCIENCE CHINA-CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Ousama Al Shanaa, Andrey Rumyantsev, Elena Sambuk, Marina Padkina
Summary: This review discusses the early stages, main developments, and latest technologies of RNA aptamer research, emphasizing the advantages of RNA aptamers compared to antibodies and their potential applications in the near future.
Article
Chemistry, Applied
Jia-Yue Tian, Xiaolong Liu, Shuai Zhang, Kun Chen, Lei Zhu, Yingpan Song, Minghua Wang, Zhihong Zhang, Miao Du
Summary: We propose a novel electrochemical aptasensing strategy for efficient detection of staphylococcus aureus (S. aureus) using a copper-based metal-organic framework encapsulated with copper oxide nanocrystals. The developed biosensor exhibits a low limit of detection for S. aureus and demonstrates high stability, reproducibility, and practicality.
Article
Biotechnology & Applied Microbiology
Natan De Jesus Pimentel-Filho, Stephan Fuchs, Maria Cristina Baracat-Pereira, Hilario Cuquetto Mantovani, Katharina Riedel, Maria Cristina Dantas Vanetti
Summary: This study investigates the stress response of Staphylococcus aureus to bovicin HC5 using a proteomic approach. The results show that sublethal concentrations of the bacteriocin repress protein synthesis, leading to growth reduction in the bacteria, while inducing certain proteins related to energy-efficient catabolism. This knowledge can help identify potential targets for controlling this pathogen.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Shuo Yao, Chao Zhao, Mingyu Shang, Juan Li, Juan Wang
Summary: A one-step fluorometric strategy based on nanometal surface energy transfer was developed for facile detection of Staphylococcus aureus. The method controlled the fluorescence signal through nucleic acid hybridization and competitive binding of aptamer, resulting in improved detection limit and linear range for S. aureus. This enzyme-free and label-free strategy shows promise for sensitive and selective S. aureus detection.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaoqi Tao, Ziyi Liao, Yaqing Zhang, Fei Fu, Mengqi Hao, Yang Song, Erqun Song
Summary: A one-step FRET sensor was developed for specific and sensitive detection of Staphylococcus aureus in food and serum samples. The sensor exhibited a good detection range and low detection limit, as well as good recovery rates and low relative standard derivations in real samples.
CHINESE CHEMICAL LETTERS
(2021)
Article
Agriculture, Dairy & Animal Science
Mei Deng, Yutong Wang, Guanhua Chen, Ju Liu, Zhengzheng Wang, Hengyi Xu
Summary: The study established a poly-L-lysine-functionalized magnetic beads (PLL-MB) strategy combined with a PCR assay for the detection of Staphylococcus aureus, achieving high capture efficiency in milk samples. The method showed a lower limit of detection, simplicity of operation, and cost-effectiveness, demonstrating its potential applications in foodborne pathogen detection.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)