期刊
SCIENCE ADVANCES
卷 6, 期 24, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aaz5057
关键词
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资金
- Spanish Ministry of Economy and Competitiveness (MINECO)/Agencia Estatal de Investigacion (AEI) [SAF2016-76190-R]
- European Regional Development Fund (ERDF, European Union)
- la Caixa Banking Foundation [HR18-00267]
- FPU fellowship from the Spanish Ministry of Education, Culture and Sports [FPU014/02456]
- EMBO Short-Term Fellowship [7732]
- Spanish Ministry of Economy and Competitiveness [BES-2017-081079]
- Secretaria d'Universitats i Recerca del Departament d'Economia i Creixement, Generalitat de Catalunya
- MINECO [SAF2016-80655-R]
- Wellcome [100993/Z/13/Z]
- Fundacion Ramon Areces
- Spanish Ministry of Science and Innovation through the Centro de Excelencia Severo Ochoa 2019-2023 Program [CEX2018-000806-S]
- Generalitat de Catalunya through the CERCA Program
Malaria transmission requires that some asexual parasites convert into sexual forms termed gametocytes. The initial stages of sexual development, including sexually committed schizonts and sexual rings, remain poorly characterized, mainly because they are morphologically identical to their asexual counterparts and only a small subset of parasites undergo sexual development. Here, we describe a system for controlled sexual conversion in the human malaria parasite Plasmodium falciparum, based on conditional expression of the PfAP2-G transcription factor. Using this system, similar to 90 percent of the parasites converted into sexual forms upon induction, enabling the characterization of committed and early sexual stages without further purification. We characterized sexually committed schizonts and sexual rings at the transcriptomic and phenotypic levels, which revealed down-regulation of genes involved in solute transport upon sexual commitment, among other findings. The new inducible lines will facilitate the study of early sexual stages at additional levels, including multiomic characterization and drug susceptibility assays.
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