Article
Food Science & Technology
Sara Kharazmi, Asghar Taheri-Kafrani
Summary: An efficient method for enhancing fruit juice quality is achieved by simultaneously hydrolyzing polysaccharides. Covalent co-immobilization of pectinase and xylanase onto functionalized iron oxide nanoparticles is reported as an efficient approach to fabricate a novel nanobiocatalyst for fruit juice clarification. The co-immobilized enzymes showed improved thermal and pH stabilities compared to free counterparts, with more than 74% and 48% of residual activity retained after six consecutive cycles for pectinase and xylanase, respectively. The developed nanobiocatalyst achieved a 53% reduction in turbidity of pineapple juice after 120 minutes treatment, and more than 64% of the initial enzyme activity was retained after nine reuses, indicating its potential application in industrial juice processing.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Seyedeh Zahra Azimi, Seyed Saeid Hosseini, Faramarz Khodaiyan
Summary: This study examined the continuous clarification of grape juice using pectinase enzymes immobilized on glass beads in a packed bed reactor. The immobilization of enzymes decreased specific activity and volumetric productivity, altered optimum pH, but had no effect on optimum temperature. The best conditions for clarification were found to be a flow rate of 0.5 mL/min and a treatment time of 42 minutes.
Article
Biotechnology & Applied Microbiology
Matt Ridge, Stephan Sommer, Daniel A. Dycus
Summary: Winemakers utilize technical enzymes for clarification, extraction, and other processes in winemaking, but their effectiveness can be influenced by various factors. This study characterizes difficult-to-clarify juices from the Muscat family, examining the impact of pasteurization while classifying the juices based on different factors. The research suggests that juice clarification is influenced by a combination of berry proteins, polysaccharides, native enzymes, and mechanical forces, highlighting the need for adjustments in winemaking strategies and enzyme use.
FERMENTATION-BASEL
(2021)
Article
Food Science & Technology
Pamela M. da Silva, Eli Emanuel Esparza-Flores, Anike H. Virgili, Eliana W. de Menezes, Roberto Fernandez-Lafuente, Lucas Dal Magro, Rafael C. Rodrigues
Summary: This study investigated the impact of different support matrices and active groups on the nutritional properties of orange juice after clarification. The results showed that immobilized enzyme on chitosan activated using genipin exhibited the highest catalytic activity and effectively reduced the turbidity of orange juice. The enzymatic treatment also maintained the natural sugar content, total soluble solids, and pH of the juice but caused color changes.
Article
Food Science & Technology
Seyed Saeid Hosseini, Faramarz Khodaiyan, Seyed Mohammad Mousavi, Seyedeh Zahra Azimi
Summary: The study found that enzymes immobilized with different cross-linkers performed well in the continuous clarification process of pomegranate juice. The clarification process significantly affected the properties of pomegranate juice, reducing turbidity and increasing clarity, while also impacting the chemical properties of the juice.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Food Science & Technology
Feng Wang, Hui Xu, Miaomiao Wang, Xiaolei Yu, Yi Cui, Ling Xu, Anzhou Ma, Zhongyang Ding, Shuhao Huo, Bin Zou, Jingya Qian
Summary: This article provides a systematic review of the methods of enzyme immobilization and the use of immobilized supports in juice clarification. It gives detailed explanations of the mechanisms and effects of immobilized pectinase, immobilized laccase, and immobilized xylanase in fruit juice clarification. Suggestions and prospects for future studies in this area are also provided.
Article
Biochemistry & Molecular Biology
Mayur R. Ladole, Pravin B. Pokale, Vrushali R. Varude, Prasad G. Belokar, Aniruddha B. Pandit
Summary: In this study, enzymes pectinase and naringinase were co-immobilized on chitosan coated magnetic nanoparticles, resulting in a biocatalyst with improved thermal stability and recyclability. The newly synthesized biocatalyst showed promising potential for applications in juice clarification and debittering processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Gul Ozyilmaz, Ecenur Gunay
Summary: This study investigated the use of co-immobilized amylase, pectinase, and cellulase enzymes for clarifying apple, grape, and pear juices. Four different co-immobilization methods were applied, and the highest activity was observed for enzymes co-immobilized onto silica gel. Response Surface Methodology (RSM) and Design Expert Software were used to optimize the enzyme units, reaction temperature, and time for turbidity and reducing sugar concentration. Batch reactor efficiency was found to be higher compared to the feedback packed bed column reactor. Changes in turbidity, reducing sugar concentrations, and antioxidant activities were observed over time during the interaction of freshly squeezed juices with co-immobilized enzymes.
Article
Biotechnology & Applied Microbiology
Kelly Menezes Macedo, Raquel Araujo Azevedo, Erik Galvao Paranhos da Silva, Thiago Pereira das Chagas, Luiz Carlos Salay, Ana Paula Trovatti Uetanabaro, Elizama Aguiar-Oliveira, Andrea Miura da Costa
Summary: Pectinases from Penicillium rolfsii CCMB 714 cultivated in jackfruit seed meal were evaluated for their potential in agroindustrial waste saccharification and orange juice clarification. The results showed that the crude extract of pectinase had optimal activity at pH 3.0-5.0 and 55 degrees C. The saccharification of cocoa husks, mangosteen husks, and passion fruit husks resulted in a significant increase in reducing sugars, and the pectinase also showed potential in clarifying orange juice.
FERMENTATION-BASEL
(2023)
Article
Microbiology
Setegn Haile, Chandran Masi, Mesfin Tafesse
Summary: The study isolated pectinase-producing bacteria from avocado peel waste, with the bacterium Serratia marcescens showing the best results. The pectinase isolated from Serratia marcescens can clarify fruit juices, with lemon juice exhibiting the highest antioxidant activity and total acidity.
Article
Biochemistry & Molecular Biology
Dilek Alagoz, Nazli Ece Varan, Ali Toprak, Deniz Yildirim, S. Seyhan Tukel, Roberto Fernandez-Lafuente
Summary: In this study, biocatalysts of immobilized xylanase were prepared using different immobilization methods and successfully applied in the clarification process of orange juice. Among them, Xyl/Si-Glu and Xyl/Si-COOH showed better potential in improving the clarity of orange juice.
PROCESS BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Priyanka Sheladiya, Chintan Kapadia, Vimal Prajapati, Hesham Ali El Enshasy, Roslinda Abd Malek, Najat Marraiki, Nouf S. S. Zaghloul, R. Z. Sayyed
Summary: This study isolated alkaline-tolerant and moderate-temperature pectate lyase-producing bacteria and investigated the characteristics and influencing factors of the enzyme under different conditions. The results showed that the pectate lyase from this strain has potential for industrial applications, particularly in the clarification of fruit juices.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Applied
Lucas Dal Magro, Joao Pedro S. Pessoa, Manuela P. Klein, Roberto Fernandez-Lafuente, Rafael C. Rodrigues
Summary: This study immobilized a commercial enzyme cocktail for fruit juice clarification on chitosan beads and found that despite low activity recovery after immobilization, the stability of the immobilized enzyme was greater than that of the free enzyme. The enzyme retained higher activity levels under extreme conditions, and the fluidized-bed reactor showed better juice clarification capacity compared to the packed-bed reactor.
Review
Food Science & Technology
Vashishtha B. Patel, Somak Chatterjee, Abhishek S. Dhoble
Summary: Pectinase enzymes are biological compounds that degrade pectic compounds in fruit juices, making them excellent biocatalysts for juice clarification. However, the poor stability and nonreusability of pectinases limit their effectiveness. Immobilization techniques, particularly using membranes, have been shown to enhance the performance of enzymes. This review highlights the importance of physicochemical properties and kinematic properties of pectinases in relation to juice processing parameters and discusses the potential of membrane immobilization as a support for pectinase.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Gayatri Bakshi, Laxmi Ananthanarayan
Summary: This study evaluated the potential of lemon peel powder as a reusable pectinase enzyme source in apple juice clarification. Results showed that treatment with 0.5% w/v lemon peel powder at an incubation time of 90 min produced a clarified apple juice with good sensory acceptability, making lemon peel powder an effective enzyme source for producing clear apple juice.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Engineering, Chemical
Khashayar Sarabandi, Alireza Sadeghi Mahoonak, Mahdieh Akbari
JOURNAL OF FOOD PROCESS ENGINEERING
(2019)
Article
Chemistry, Applied
Khashayar Sarabandi, Seid Mahdi Jafari, Maryam Mohammadi, Zahra Akbarbaglu, Akram Pezeshki, Maryam Khakbaz Heshmati
FOOD HYDROCOLLOIDS
(2019)
Article
Engineering, Chemical
Khashayar Sarabandi, Pouria Gharehbeglou, Seid Mahdi Jafari
Article
Biochemistry & Molecular Biology
Khashayar Sarabandi, Seid Mandi Jafari, Alireza Sadeghi Mahoonak, Adeleh Mohammadi
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Chemistry, Applied
Khashayar Sarabandi, Seid Mahdi Jafari
Article
Biochemistry & Molecular Biology
Maryam Mohammadi, Sanam Mirabzadeh, Rahim Shahvalizadeh, Hamed Hamishehkar
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Agriculture, Multidisciplinary
Khashayar Sarabandi, Seid Mahdi Jafari
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Engineering, Chemical
Khashayar Sarabandi, Seid Mahdi Jafari
JOURNAL OF FOOD ENGINEERING
(2020)
Article
Chemistry, Applied
Rahim Shahvalizadeh, Raman Ahmadi, Iskandar Davandeh, Akram Pezeshki, Seyed Amir Seyed Moslemi, Sanaz Karimi, Mahdi Rahimi, Hamed Hamishehkar, Maryam Mohammadi
Summary: The addition of 1%, 3%, and 5% zinc oxide nanoparticles to gelatin and tragacanth resulted in antimicrobial nanocomposites with increased melting point temperature, ultimate tensile strength, and Young's Modulus. These nanocomposites exhibited reduced water vapor permeability and good antimicrobial activity against Staphylococcus aureus and Escherichia coli, showing promise as bio-materials for food packaging.
Article
Biochemistry & Molecular Biology
Maryam Mohammadi, Hamed Hamishehkar, Marjan Ghorbani, Rahim Shahvalizadeh, Mirian Pateiro, Jose M. Lorenzo
Summary: Encapsulating Spirulina hydrolysate into nanoliposomes with different stabilizers and chitosan coating shows high encapsulation efficiency, antioxidant activity, and physical stability. Spray-dried powder of peptide-loaded chitosomes maintains all characteristics, making it a potential functional food ingredient for human diet.
Article
Food Science & Technology
Khashayar Sarabandi, Adeleh Mohammadi
Summary: In this study, the phenolic extract of peppermint was successfully encapsulated within a sucrose matrix using the co-crystallization process. The physical properties, total phenolic content, antioxidant properties, and morphology of the samples were evaluated. The results showed that the concentration of the loaded extract significantly affected the color, antioxidant capacity, and structure of the samples. The gummy candy formulated using the co-crystallized powders had the highest score in terms of color, texture, and taste. This study highlights the effectiveness of co-crystallization as a method for encapsulating bioactive compounds.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Pharmacology & Pharmacy
Khashayar Sarabandi, Fardin Tamjidi, Zahra Akbarbaglu, Katarzyna Samborska, Pouria Gharehbeglou, Mohammad Saeed Kharazmi, Seid Mahdi Jafari
Summary: In this study, whey protein concentrate (WPC) was modified by sonication or partial hydrolysis to enhance its properties as an emulsifier and carrier for grape seed oil (GSO)-in-water emulsions. The modified WPC showed improved solubility, emulsifying activity, and foaming activity, as well as increased antioxidant activity and amino acid content. The modified WPC-stabilized emulsions had smaller droplet size, more uniform distribution, and higher zeta potential compared to intact WPC. The corresponding spray-dried powders exhibited improved encapsulation efficiency, oxidative stability, reconstitution ability, flowability, solubility, and hygroscopicity. The study also found that the functional characteristics and antioxidant activity of the carriers influenced the morphology of the particles and oxidative stability of the GSO. Partial enzymolysis was found to be more effective than ultrasonication. This study suggests that WPC hydrolysates can be used as emulsifiers, carriers, and antioxidants for the delivery and protection of bioactive compounds.
Review
Chemistry, Applied
Zahra Akbarbaglu, Seyed Hadi Peighambardoust, Khashayar Sarabandi, Seid Mahdi Jafari
Summary: Spray-drying is a common and cost-effective technique for encapsulating nutrients and bioactive compounds, but challenges such as shear and thermal stresses, as well as physicochemical instability during storage, lead to the loss of compounds. This paper focuses on the stabilization of bioactives in carriers such as proteins, discussing their role in encapsulation and maintenance of physicochemical, structural, and functional properties of bioactive compounds and nutrients.