Article
Biotechnology & Applied Microbiology
Xuguo Duan, Qiuyu Zhu, Xinyi Zhang, Zhenyan Shen, Yue Huang
Summary: The study revealed that the beta-amylase from Bacillus aryabhattai has high specific activity and specificity, making it a potential candidate for industrial production of maltose from starch.
MICROBIAL CELL FACTORIES
(2021)
Article
Agricultural Engineering
Sulogna Chatterjee, S. Venkata Mohan
Summary: The study evaluates the potential of different vegetable wastes as an alternative feedstock for the production of renewable sugars and bioethanol. Through thermal assisted chemical pretreatment and enzymatic saccharification, sugars were successfully converted into ethanol, with potential positive impacts on the economic and sustainability of the circular biorefinery process.
BIORESOURCE TECHNOLOGY
(2021)
Review
Agricultural Engineering
David Alexander Mitchell, Isabelle Moreira, Nadia Krieger
Summary: Stochastic models have the potential to be important tools in describing the complex network of reactions involved in enzymatic hydrolysis of polysaccharides, but many current models lack biologically meaningful parameters. Only a few models utilize classical enzyme kinetics parameters, and recent research on stochastic models for cellulose hydrolysis provides valuable insights into the molecular-level phenomenon limiting hydrolysis rates. Further improvements are necessary for stochastic models of enzymatic polysaccharide hydrolysis to become widely used tools for guiding process development.
BIORESOURCE TECHNOLOGY
(2021)
Article
Energy & Fuels
Tooba Khan, Muhammad Sohail, Syed Tariq Ali
Summary: Halophytic biomass from Arthrocnemum macrostachyum was utilized as a substrate to produce multienzyme preparation, and the results showed that it can be used for industrial enzyme production.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Polymer Science
Yen-Ching Yang, Wei-Shen Huang, Shu-Man Hu, Chao-Wei Huang, Chih-Hao Chiu, Hsien-Yeh Chen
Summary: A regulatable bioremediation capsule material was fabricated using a vapor-phased encapsulation method with isolated single-strain bacteria (Bacillus species, B. CMC1) and a regulator molecule (carboxymethyl cellulose, CMC) to enhance bacterial growth and enzymatic activity for cellulase activation. The encapsulation process demonstrated scalability, simplicity, and effectiveness in enhancing specific enzyme functions for bioremediation applications.
Article
Biotechnology & Applied Microbiology
Xiaodong Zhang, Caixia Li, Xuantong Chen, Chonlong Chio, Sarita Shrestha, Wensheng Qin
Summary: The study identified and characterized a high alpha-amylase producing bacterium WangLB, and cloned its alpha-amylase coding gene. The characterized BvAmylase showed good stability and activity, making it a potential candidate enzyme for industrial applications.
FERMENTATION-BASEL
(2021)
Article
Biophysics
Asad Karim, Zainab Bibi, Haneef Ur Rehman, Afsheen Aman, Shah Ali Ul Qader, Muhammad Hamid Rashid
Summary: In this study, CMCase from Bacillus licheniformis KIBGE-IB2 was immobilized within agarose gel matrix, leading to improved catalytic properties with reduced maximum reaction rate and increased Michaelis-Menten constant. The immobilized enzyme showed enhanced stability and could be reused up to eight reaction cycles, indicating its potential application in various industrial processes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Applied
Jianhua Yi, Manyan Qiu, Zhenbao Zhu, Xinling Dong, Eric Andrew Decker, David Julian McClements
Summary: A novel magnetic nanobiocatalyst was developed for extracting anthocyanins from black rice, demonstrating higher stability and greater catalytic activity compared to free enzymes, with good thermal and pH stability. The maximum yield of 266 mg anthocyanin/100 g black rice was obtained, and after six reuse cycles, cellulase and alpha-amylase retained around 70% and 64% of their activity, respectively.
Review
Food Science & Technology
Dilshad Begum M. Golgeri, Sikandar Mulla, Zabin K. Bagewadi, Swati Tyagi, Anyi Hu, Swati Sharma, Muhammad Bilal, Ram Naresh Bharagava, Luiz Fernando R. Ferreira, Dummi Mahadevan Gurumurthy, Ashok Kumar Nadda
Summary: Microbial enzymes, particularly carbohydrases, have gained significant attention due to their stability and potential applications in areas such as bioenergy, biofuel production, and biobleaching. Isolating novel microbes from harsh environments has become a major focus for scientists. This review highlights the importance and properties of carbohydrases, specifically amylase, cellulase, laccase, pectinase, and xylanase, and explores their applications, production optimization, molecular approaches for enhanced production, and future biotechnological perspectives. Green and sustainable catalytic conversion strategies using enzyme nanoparticles are also discussed.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Environmental Sciences
T. Indumathi, Rita Jayaraj, P. Senthil Kumar, Mary Isabella Sonali, Veena Gayathri Krishnaswamy, Ayman A. Ghfar, Saravanan Govindaraju
Summary: This study successfully achieved enzymatic de-inking of photocopy papers by isolating bacteria and extracting cellulase enzyme, providing a biological approach for the recycling of paper.
Article
Environmental Sciences
Veena Gayathri Krishnaswamy, Rajalakshmi Sridharan, P. Senthil Kumar, Mariyam Jaffer Fathima
Summary: The study focused on isolating bacterial strains Bacillus pacificus and Pseudomonas mucidolens from vermicompost, extracting cellulase enzyme and determining its molecular weight using SDS-PAGE. The maximum enzyme activity reported was 0.12 U/mL and 0.17 U/mL after dialysis. LDPE degradation was observed up to 30 days, with FTIR analysis showing peak changes in the process. The bacterial strains were identified using Scanning Electron Microscopy and 16S rRNA sequencing.
Article
Agriculture, Dairy & Animal Science
Z. K. Liu, Y. Li, C. C. Zhao, Z. J. Liu, L. M. Wang, X. Y. Li, W. F. Pellikaan, J. H. Yao, Y. C. Cao
Summary: This study aimed to investigate the effects of adding a combination of fibrolytic and amylolytic enzymes to the diet of early-lactation dairy cows. The results showed that enzyme supplementation increased rumen enzyme activity, improved milk performance, and had a tendency to improve feed efficiency. However, there was no significant difference in rumen bacterial richness and diversity between the control and experimental groups.
Article
Biotechnology & Applied Microbiology
Othman M. Alzahrani, Muhammad Sohail, Samy F. Mahmoud, Amal S. Alswat, Yasser El-Halmouch
Summary: Enzyme-mediated degradation of sugarcane bagasse by bacterial strains was studied. The bacterial consortium consisting of B. thuringiensis B45, B. velezensis BF3, and B. amyloliquefaciens B987 was able to produce endoglucanase, xylanase, and pectinase, respectively. The results demonstrated the potential of solid-state fermentation to obtain industrially important enzymes.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Applied
Bin He, Pengbing Chang, Xing Zhu, Sufeng Zhang
Summary: Construction of a biomimetic cellulase-loaded enzymatic film inspired by the sea anemone's predation process can effectively increase the efficiency of insoluble cellulose hydrolysis and enable enzyme recycling. This immobilization system can significantly reduce the cost of cellulose conversion in industrial areas.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Nanzhu Chen, Shuo Yang, Dawei You, Junfeng Shen, Banlai Ruan, Mei Wu, Jianzhi Zhang, Xiaozhou Luo, Hongting Tang
Summary: This study systematically investigated the effect of engineering cell wall biosynthesis on the activity of cellulolytic enzymes and found that the inactivation of certain genes significantly improved the secretion and surface-display of β-glucosidase. Furthermore, combinatorial modifications of these genes, along with the use of rich medium, greatly increased the activity of the enzymes. Proteomic analysis also revealed the involvement of translation processes in improving enzyme activity through cell wall biosynthesis engineering.
METABOLIC ENGINEERING
(2023)
Article
Agricultural Engineering
Mingpu Du, Dong Xu, Xuantung Trinh, Shuangyuan Liu, Mei Wang, Yi Zhang, Junmei Wu, Qiaohong Zhou, Zhenbin Wu
BIORESOURCE TECHNOLOGY
(2016)
Article
Ecology
Chuan Wang, Shuangyuan Liu, Yi Zhang, Biyun Liu, Lei Zeng, Feng He, Qiaohong Zhou, Zhenbin Wu
Article
Ecology
Chuan Wang, Shuangyuan Liu, Yi Zhang, Biyun Liu, Feng He, Dong Xu, Qiaohong Zhou, Zhenbin Wu
Article
Ecology
Lu Du, Xuantung Trinh, Qianru Chen, Chuan Wang, Shuangyuan Liu, Panpan Liu, Qiaohong Zhou, Dong Xu, Zhenbin Wu
ECOLOGICAL ENGINEERING
(2018)
Article
Mathematics, Interdisciplinary Applications
Chun Wang, Xue Zhang
MULTIVARIATE BEHAVIORAL RESEARCH
(2019)
Article
Microbiology
Jingcheng Dai, Yaqi Liu, Shuangyuan Liu, Shuyang Li, Na Gao, Jing Wang, Jizhong Zhou, Dongru Qiu
Article
Fisheries
Md. Shahdat Hossain, Jingcheng Dai, Dongru Qiu
Summary: AHPND, caused by Vibrio parahaemolyticus, is a severe hepatopancreatic disease of shrimp, linked to gut bacterial imbalance and selective pressure on microbiota. The presence of pirA(Vp) toxin alone is sufficient to alter the core microbiota of infected shrimp. Infected shrimp show enrichment in Firmicutes and Bacteroidetes, while Proteobacteria levels decrease. Significant differences in SCFAs-producing species were found between healthy and infected shrimp gut.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Md. Shahdat Hossain, Jingcheng Dai, Dongru Qiu
Summary: The study on gut microbial communities in European eels revealed that eels from different farms harbor distinct gut microbiota, potentially influenced by both rearing conditions and host physiology. Additionally, the gut bacterial community of eels was significantly impacted by the microbiota of supplied feed and contiguous water sources.
AQUACULTURE INTERNATIONAL
(2021)
Article
Microbiology
Shirin Sultana, Md. Nasir Khan, Muhammad Shahdat Hossain, Jingcheng Dai, Mohammad Shamsur Rahman, Md. Salimullah
Summary: This study investigated the skin microbiomes and their functional annotations in healthy and diseased stinging catfish using 16S rRNA metagenomics. The results revealed differences in the composition, richness, and functions of the skin microbiome between healthy and diseased fish. The findings suggest that the bacterial community can influence the health status of cultured stinging catfish.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Ecology
Chuan Wang, Shuangyuan Liu, Tahmina E. Jahan, Biyun Liu, Feng He, Qiaohong Zhou, Zhenbin Wu
ECOLOGICAL ENGINEERING
(2017)