Biosynthesis of copperoxide nanoparticles using Abies spectabilis plant extract and analyzing its antinociceptive and anti-inflammatory potency in various mice models

Nanotechnology has shown rapid progress in various fields had made a remarkable change in the field of therapeutics too. The present study aims to synthesize and characterize the eco-friendly biocompatible copper oxide nanoparticles using Abies spectabilis plant extract (ASCuONPs) and inspect its ameliorative potential against the different stimuli induced nociception and inflammatory reactions in mice. The formulated AS-CuONPs were characterized via the UV-vis spectroscopy, FT-IR, and TEM analysis. The nociception was induced by the method of acetic acid, glutamate, capsaicin, formalin, and thermal-induced techniques. The antiinflammatory property of AS-CuONPs with carrageenan induced paw edema, peritoneal leukocyte infiltration, and carrageenan induced air pouch tests. Our result of UV-Visible spectroscopic analysis confirms the formation of AS-CuONPs and the characterization with FTIR and TEM proves the same. The result of various stimuli induced nociception models provide the evidence that formulated AS-CuONPs effectively inhibits the nociceptive responses in mice. Further the infiltration of leukocytes and inflammatory cytokines were drastically decreased by the AS-CuONPs treatment in the mice. The open field analysis confirms that the AS-CuONPs doesn't impart any behavioral changes in mice. Over all our results confirms that AS-CuONPs is effective against different stimuli induced nociception and it act as a potent anti-inflammatory agent without rendering any side effects. (c) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).