Article
Chemistry, Analytical
Dario Brambilla, Laura Sola, Anna Maria Ferretti, Elisa Chiodi, Natasa Zarovni, Diogo Fortunato, Mattia Criscuoli, Vincenza Dolo, Ilaria Giusti, Valentina Murdica, Liliana Czernek, Markus Duchler, Riccardo Vago, Marcella Chiari, Katarzyna Kluszczyriska
Summary: Extracellular vesicles (EVs) play an important role in cell-cell communication, disease diagnosis, and drug delivery, but there is no consensus on the best method for separating micro- and nanovesicles from cell culture supernatant and complex biological fluids. Immunoaffinity capture methods provide high-purity samples and address issues with current separation methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lijun Xu, Siyu Lu, Hua Wang, Huiying Xu, Bang-Ce Ye
Summary: This study presents a highly sensitive detection method for identifying glycosylated exosomal proteins based on a proximity ligation assay-induced rolling circle amplification strategy. The proposed strategy allows for accurate quantification of specific glycosylated exosomal proteins by converting different levels of protein-specific glycosylation into changes in absorbance signals. Furthermore, the strategy demonstrates the potential correlation between exosomal glycosylation and cell polarization.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Masaru Kato, Riho Nakamoto, Masaki Ishizuka, Noriko Watanabe
Summary: sEVs, carrying DNA, miRNA, and proteins, are important for intercellular communication and can be used as biomarkers for disease diagnosis and drug delivery. This study identified cationic particles that effectively purify sEVs and proposed a convenient purification method, which is expected to facilitate functional analysis and disease treatment using sEVs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lin Shi, Haiying Cai, Han Wang, Qiwei Wang, Lili Shi, Tao Li
Summary: In this study, a novel method based on the FNA nanoplatform is proposed for the effective capture of exosomes and direct detection of internal miRNAs. The results show that miR-21 has a negative regulatory role in apoptosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Yijun Zhou, Runjie Yuan, Allaura S. Cone, Kyle W. Shifflett, Gabriel F. Arias, Alice Peng, Meredith G. Chambers, Ryan P. McNamara, Smaranda Willcox, Justin T. Landis, Yue Pan, Jack Griffith, Dirk P. Dittmer
Summary: Purification of extracellular vesicles (EVs) has been a challenge due to their heterogeneous cargo. However, a new study demonstrates that heparin chromatography can successfully separate purified EVs into two distinct subpopulations - a non-heparin-binding fraction containing classic EV markers and a heparin-binding fraction enriched in fibronectins and histones. These fractions have different effects on endothelial cells, emphasizing the importance of this novel fractionation step for improving clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Engineering, Biomedical
Zhengyu Yang, Xiaodong Liu, Fujian Zhao, Mengyu Yao, Zefeng Lin, Zhen Yang, Cong Liu, Yuwei Liu, Xiaofeng Chen, Chang Du
Summary: This study reveals a new mechanism by which bioactive glass nanoparticles (BGN) regulate bone loss through inducing the secretion of extracellular vesicles (EVs) by bone marrow mesenchymal stem cells (BMSCs). The EVs inhibit osteoclast differentiation by reducing the nuclear translocation of NFATc1. Furthermore, BGN+BMSC-EVs show potential in alleviating bone loss in an osteoporosis mouse model, with no acute systemic toxicity.
Review
Biotechnology & Applied Microbiology
Kecheng Lou, Shangzhi Feng, Hui Luo, Junrong Zou, Guoxi Zhang, Xiaofeng Zou
Summary: Macrophages and their extracellular vesicles play significant roles in tumor microenvironment and cancer development, and show potential for clinical applications in immunomodulation, cancer therapy, infectious defense, and tissue repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Food Science & Technology
Huifan Liu, Jiaxi Liang, Xia Ye, Miaoru Huang, Lukai Ma, Xi Xie, Dongjie Liu, Hui Cao, Jesus Simal-Gandara, Kannan R. R. Rengasamy, Qin Wang, Gengsheng Xiao, Jianbo Xiao
Summary: Recent studies have shown that extracellular vesicles (EVs) have great potential in human health. EVs contain natural bioactive compounds, such as lipids, proteins, and RNA, which regulate biological processes and can yield synergistic, preventive, and therapeutic effects. This review comprehensively elucidates the relationship between EVs and bioactive compounds, discussing different forms of biological regulation and methods to load bioactive compounds into EVs.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Engineering, Biomedical
Hongtao Xu, Yanlun Zhu, Allen Wei-Ting Hsiao, Jiankun Xu, Wenxue Tong, Liang Chang, Xuerao Zhang, Yi-Fan Chen, Jie Li, Wei Chen, Yingze Zhang, Hon Fai Chan, Chien-Wei Lee
Summary: The use of MSC-EVs boosted with bioactive glasses (EVB) shows great potential in treating tendon injuries by promoting angiogenesis and enhancing anti-inflammatory responses of M2 macrophages. EVB can significantly improve the biomechanical properties and promote functional regeneration of the repaired tendon.
Article
Chemistry, Multidisciplinary
Kaizhe Wang, Yuhan Wei, Xiaodong Xie, Qian Li, Xiaoguo Liu, Lihua Wang, Jiang Li, Ji Wu, Chunhai Fan
Summary: This study presents a method for encoding the heterogeneous interfaces of extracellular vesicles (EVs) and cells using DNA, enabling interspecies EV-cell communications in complex environments. The use of cholesterol-modified DNA strands and tetrahedral DNA frameworks allows specific and efficient cellular internalization of EVs via base pairing. The system demonstrates orthogonal EV-cell communications and efficient delivery of bioactive contents derived from feeder cells to target cells.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Zeljka Persuric, Sandra Kraljevic Pavelic
Summary: Recent research has focused on identifying health-promoting compounds in bee products such as honey, royal jelly, propolis, and bee pollen. Mass spectrometry techniques have revealed the diversity in chemical composition of bee products, influenced by factors like botanical sources and geographical origin. Extracellular vesicles from bee products show promise as a novel bioactive nutraceutical component, with potential applications in wound healing and tissue regeneration.
Article
Engineering, Biomedical
Han Liu, Qin Zhang, Sicheng Wang, Weizong Weng, Yingying Jing, Jiacan Su
Summary: This review summarizes the mechanisms of formation, composition, isolation and purification techniques, as well as the application of bacterial extracellular vesicles (BEVs) as drug delivery platforms. The authors believe that a deeper understanding of BEVs will open new opportunities for their development in drug delivery applications.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Analytical
Lu Liu, Ruilin Yang, Jiaxuan Cui, Peng Chen, Hyok Chol Ri, Huaze Sun, Xiangfan Piao, Minshu Li, Qiaosheng Pu, Maurizio Quinto, John L. Zhou, Hai-Bo Shang, Donghao Li
Summary: This study proposes a circular nonuniform electric field strategy coupled with gel electrophoresis to achieve precise separation and efficient concentration of nano- and microparticles. By controlling the distribution of electric field lines in functional zones, the migration behaviors of particles can be regulated, enabling their separation and concentration. This method provides a new insight into simultaneous particle separation and concentration, offering a versatile tool for sample preparation and separation.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Yinzhu Lu, Bingqian Lin, Weizhi Liu, Jialu Zhang, Lin Zhu, Chaoyong Yang, Yanling Song
Summary: A modular platform is developed for sequential isolation of tumor and non-tumor extracellular vesicles (EVs) using dual-aptamer recognition and tandem microchips. This method provides new clues for assessing immune heterogeneity and can be used for EV proteome profiling.
Article
Cell Biology
Rossella Crescitelli, Stefan Filges, Nasibeh Karimi, Ornella Urzi, Tamara Alonso-Agudo, Anders Stahlberg, Jan Lotvall, Cecilia Lasser, Roger Olofsson Bagge
Summary: This study identified tumor-specific DNA mutations in EVs isolated from melanoma metastatic tissues, showing a higher mutant allele frequency in DNA isolated from tumor-derived EVs compared to total plasma DNA, indicating the potential role of tumor EVs as future biomarkers in melanoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Ethan Z. Malkin, Steven De Michino, Meghan Lambie, Rita Gill, Zhen Zhao, Ariana Rostami, Andrea Arruda, Mark D. Minden, Scott V. Bratman
Summary: Cancer cells release cfDNA, which is predominantly associated with nucleosomal particles and not EVs. Additionally, a subset of mitochondrial cfDNA is membrane protected and derived from non-tumor cells. These findings have important implications for understanding the topology of cfDNA in cancer.
