5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

Versatile 3′ Functionalization of CRISPR Single Guide RNA

(2020) Cody M. Palumbo et al.   CHEMBIOCHEM

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

(2019) Kara van Aelst et al.   Genes

Improving CRISPR Genome Editing by Engineering Guide RNAs

(2019) Su Bin Moon et al.   TRENDS IN BIOTECHNOLOGY

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

Guide RNA modification as a way to improve CRISPR/Cas9-based genome-editing systems

(2019) Julia Filippova et al.   BIOCHIMIE

CRISPcut : A novel tool for designing optimal sgRNAs for CRISPR/Cas9 based experiments in human cells

(2018) Jaspreet Kaur Dhanjal et al.   GENOMICS

Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

(2018) Seuk-Min Ryu et al.   NATURE BIOTECHNOLOGY

Structure basis for RNA-guided DNA degradation by Cascade and Cas3

(2018) Yibei Xiao et al.   SCIENCE

Analysis of CRISPR-Cas System in Streptococcus thermophilus and Its Application

(2018) Mengyuan Hao et al.   Frontiers in Microbiology

3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses

(2018) Yasaman Gholamalipour et al.   NUCLEIC ACIDS RESEARCH

Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

(2017) Weixin Tang et al.   Nature Communications

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

(2017) Yavuz S. Dagdas et al.   Science Advances

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

CRISPR-Cas9 nuclear dynamics and target recognition in living cells

(2016) Hanhui Ma et al.   JOURNAL OF CELL BIOLOGY

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

(2016) Yi Fu et al.   Nature Communications

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display

(2015) David M Shechner et al.   NATURE METHODS

Genome Engineering with Targetable Nucleases

(2014) Dana Carroll   Annual Review of Biochemistry

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

(2014) Alexandra E. Briner et al.   MOLECULAR CELL

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

(2013) Tautvydas Karvelis et al.   RNA Biology

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

PNPASE and RNA trafficking into mitochondria

(2011) Geng Wang et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

(2011) Rimantas Sapranauskas et al.   NUCLEIC ACIDS RESEARCH

PNPASE Regulates RNA Import into Mitochondria

(2010) Geng Wang et al.   CELL