Article
Biotechnology & Applied Microbiology
Kristina J. Tatiossian, Robert D. E. Clark, Chun Huang, Matthew E. Thornton, Brendan H. Grubbs, Paula M. Cannon
Summary: This study found that CRISPR-Cas9 indel signatures can be used to identify gRNAs that maximize HDR outcomes, with the frequency of deletions resulting from MMEJ repair of at least 3 base pairs better predicts HDR frequency. Existing tools can be repurposed to identify gRNAs that promote HDR.
Article
Multidisciplinary Sciences
Emmarie. C. C. Ryan, Leslie. M. M. Huggins, Joshua. D. D. Podlevsky
Summary: CRISPR arrays and Cas proteins are adaptive immune systems in bacteria and archaea, defending against genetic elements. The reprogrammable guide RNA of single effector CRISPR-Cas systems greatly facilitates gene-editing but complicates PCR tests. These systems are derived from human microflora and pathogens, making detection of gene-editor exposure difficult. To overcome false positives, a DNA displacement assay has been developed to specifically detect gene-editors without cross-reacting with bacterial CRISPRs.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Min Qiu, Zachary Glass, Jinjin Chen, Mary Haas, Xin Jin, Xuewei Zhao, Xuehui Rui, Zhongfeng Ye, Yamin Li, Feng Zhang, Qiaobing Xu
Summary: Loss-of-function mutations in Angiopoietin-like 3 (Angptl3) are linked to reduced blood lipid levels, making it a potential therapeutic target for lipid metabolism disorders. A lipid nanoparticle delivery system successfully edited Angptl3 gene in vivo, leading to significant reductions in lipid levels without off-target effects or toxicity, highlighting the potential of this platform for safe and effective gene editing therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Shoko Takeuchi, Mitsuo Yamamoto, Satoru Matsumoto, Eriya Kenjo, Masatoshi Karashima, Yukihiro Ikeda
Summary: The CRISPR-Cas9 system is a promising gene editing technology that can produce specific changes for the treatment of various cancers and genetic diseases. However, there are limitations to its application, including activity without side effects, efficient delivery to target cells, and stable expression of Cas9 endonuclease.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Avantika Ghosh, Ksenia Myacheva, Marisa Riester, Carla Schmidt, Sven Diederichs
Summary: The ability to precisely alter the genome holds immense potential. This study explores the use of chemically synthesized chimeric oligonucleotides to encode a target-specific crRNA for precision genome editing. The results demonstrate the effectiveness of chimeric oligos in inducing accurate genome editing.
Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Article
Biotechnology & Applied Microbiology
Hui Kwon Kim, Goosang Yu, Jinman Park, Seonwoo Min, Sungtae Lee, Sungroh Yoon, Hyongbum Henry Kim
Summary: This study identified factors affecting PE2 efficiency through high-throughput evaluation and developed three computational models to predict pegRNA efficiency, which can be applied to edits of various types and positions. Spearman's correlations between 0.47 and 0.81 were found when testing the accuracy of the predictions using independent test data sets.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Saed Abbasi, Satoshi Uchida, Kazuko Toh, Theofilus A. Tockary, Anjaneyulu Dirisala, Kotaro Hayashi, Shigeto Fukushima, Kazunori Kataoka
Summary: Co-delivery of Cas9 mRNA and sgRNA using PEGylated polyplex micelle (PM) enhances genome editing efficiency in a variety of cell types, including brain parenchymal cells, with improved sgRNA stability and higher editing efficiency than delivery of Cas9 mRNA or sgRNA alone. This approach shows promise for in vivo genome editing and demonstrates the potential of PMs for co-encapsulation of Cas9 mRNA and sgRNA.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Ahmed K. Mahdi, Juan F. Medrano, Pablo J. Ross
Summary: We investigated the possibility of single-step genome editing in small ruminants using CRISPR-Cas9 zygote electroporation. We found that electroporation was more efficient than microinjection and successfully disrupted target genes in sheep and goat embryos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Zsolt Bodai, Alena L. Bishop, Valentino M. Gantz, Alexis C. Komor
Summary: Programmable double-strand DNA breaks (DSBs) can be harnessed for precision genome editing through manipulation of the homology-directed repair (HDR) pathway. This study introduces a general strategy called the double tap method, which improves HDR-mediated precision editing efficiency by taking advantage of the reproducible nature of indel sequences. The method utilizes multiple gRNAs, including a primary gRNA that targets the wild-type genomic sequence and one or more secondary gRNAs that target the most common indel sequence(s).
NATURE COMMUNICATIONS
(2022)
Review
Agriculture, Dairy & Animal Science
Julia Popova, Victoria Bets, Elena Kozhevnikova
Summary: Genome editing has practical applications in farm animals, improving production traits, economic value, and disease resistance. It also has potential in biomedical research and drug production, as well as xenograft donors. Recent advancements in site-specific nucleases and embryological delivery methods have revolutionized transgenesis, providing efficient and reliable tools for genome engineering in agriculture.
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Dusko Lainscek, Vida Forstneric, Veronika Mikolic, Spela Malensek, Peter Pecan, Mojca Bencina, Matjaz Sever, Helena Podgornik, Roman Jerala
Summary: The CRISPR/Cas system, when coupled with Exonuclease III, can enhance gene editing efficiency and lead to significant gene knock-out effects, especially in cell lines, primary cells, and in vivo. This approach has great potential for treating diseases with a genetic etiology.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Jeong Sun Lee, Su-Ji Bae, Jin-Soo Kim, Changsoo Kim, Beum-Chang Kang
Summary: In this study, we optimized a system for predicting the activity of sgRNAs for genome editing in sorghum, which is efficient and straightforward. This system will reduce the time and effort required for sorghum genome editing.
Article
Microbiology
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai
Summary: Bacteriophages, the most abundant organisms in the biosphere, have been sequenced extensively. However, the study of bacteriophage functional genomics has been hindered by a lack of effective research methods. This study designed a phage genome editing platform based on the CRISPR-Cas9 system, and successfully achieved gene editing in Vibrio natriegens phage TT4P2. This platform has the potential to advance research on phage gene diversity and accelerate the development of phage synthetic biology and nanotechnology.
MICROBIOLOGY SPECTRUM
(2022)
Article
Genetics & Heredity
Andrey Komissarov, Mariia Sergeeva, Evgenii Zhuravlev, Sergey Medvedev, Anastasia Malakhova, Evgeniya Andreeva, Anna-Polina Shurygina, Andrey Gorshkov, Mariia Timofeeva, Evgenia Balakhonova, Mikhail Grudinin, Suren Zakian, Vladimir Richter, Grigory Stepanov
Summary: This study successfully overcame host restriction factors and increased the susceptibility of human cell lines to influenza infection by knocking out the ANXA6 gene using CRISPR-Cas9. The results showed that the accumulation of influenza A virus in cells lacking the ANXA6 gene significantly exceeded the virus titer in the original cells, indicating higher replication capacity.
Article
Biochemistry & Molecular Biology
Polina V. Zhdanova, Alexander A. Chernonosov, Daria V. Prokhorova, Grigory A. Stepanov, Lyubov Yu. Kanazhevskaya, Vladimir V. Koval
Summary: This study presents the first attempt to describe the solution structure of Cas9 from S. pyogenes using hydrogen-deuterium exchange mass spectrometry (HDX-MS) coupled to molecular dynamics simulations. The study identifies peptides involved in complex formation and possible changes in protein conformation induced by sgRNA binding. The results provide new insights into the functioning of Cas9 and demonstrate the practical utility of HDX-MS for studying Cas9 mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Virology
Daria Prokhorova, Natalya Zhukova (Eschenko), Anna Lemza, Mariia Sergeeva, Rinat Amirkhanov, Grigory Stepanov
Summary: This review examines the impact of the genome editing system on specific target genes in cells (human and chicken), and its effects on the influenza virus life cycle and virus particle production efficiency.
Review
Biochemistry & Molecular Biology
Anton Dome, Maya Dymova, Vladimir Richter, Grigory Stepanov
Summary: This review focuses on the alterations in post-transcriptional maturation of RNA in human glioblastoma cells, which disrupts the normal process of apoptosis. The review provides a comprehensive overview of the latest insights into post-transcriptional modifications of specific regulatory RNAs associated with apoptosis, presents findings on the characteristics of apoptosis in glioblastoma cells, and highlights the connection between regulatory RNAs and apoptosis in tumor cells. In conclusion, potential target candidates for the development of new drugs for glioblastoma treatment are identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Polina V. Zhdanova, Alexander A. Lomzov, Daria V. Prokhorova, Grigory A. Stepanov, Alexander A. Chernonosov, Vladimir V. Koval
Summary: This article investigates the thermodynamics of the interaction between Cas9-RNA and DNA/DNA duplex and reveals its importance in the CRISPR-Cas9 system. The study findings suggest that the small energetic preference during Cas9-RNA/DNA formation may play a crucial role in sequence-specific recognition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Dmitry Selishchev, Grigory Stepanov, Mariia Sergeeva, Maria Solovyeva, Evgenii Zhuravlev, Andrey Komissarov, Vladimir Richter, Denis Kozlov
Summary: This paper describes the photoinduced antiviral effect of cotton fabric functionalized with nanocrystalline TiO2. The photoactive fabric can effectively inactivate influenza A (H1N1) virus through adsorption and photocatalytic degradation mechanisms. This study highlights the potential of photoactive self-cleaning fabrics for air purification and personal protective clothing to protect against chemical and biological pollutants.
Article
Cell Biology
Natalia S. Vasileva, Elena Kuligina, Maya A. Dymova, Yulya Savinovskaya, Nikita D. Zinchenko, Alisa B. Ageenko, Sergey Mishinov, Anton S. Dome, Grigory A. Stepanov, Vladimir A. Richter, Dmitry Semenov
Summary: Glioma, the most common and heterogeneous primary brain tumor, requires the development of new preclinical models. Comparing adherent and neurosphere cell cultures, this study revealed the activation of specific transcription factors during neurosphere formation and identified common signaling feedback regulators in gliomas for potential therapeutic strategies against invasion and progression.
Article
Biochemistry & Molecular Biology
Evgenii Zhuravlev, Mariia Sergeeva, Sergey Malanin, Rinat Amirkhanov, Dmitriy Semenov, Tatiana Grigoryeva, Andrey Komissarov, Grigory Stepanov
Summary: Small nucleolar RNAs (snoRNAs) are a class of highly expressed non-coding RNAs known for their role in post-transcriptional modifications. Recent studies have shown their involvement in various cellular processes and diseases, including cancer and viral infections. A study on the expression pattern of snoRNAs during influenza A viral infection found significant alterations, suggesting their potential role in the virus replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Daria V. V. Prokhorova, Ivan P. Vokhtantsev, Polina O. Tolstova, Evgenii S. Zhuravlev, Lilia M. Kulishova, Dmitry O. Zharkov, Grigory A. Stepanov
Summary: In this study, we show that incorporating N-6-methyladenosine, 5-methylcytidine, and pseudouridine modifications into guide RNAs enhances the efficiency and specificity of the CRISPR-Cas9 system. We found that the complexes of modified guide RNAs with Cas9 protein promote cleavage of target duplexes and plasmid substrates, while reducing immunostimulatory and cytotoxic effects of sgRNAs.
Review
Biochemistry & Molecular Biology
Denis N. Antropov, Grigory A. Stepanov
Summary: Nucleic acid detection assays are widely used in investigating specific sequences and identifying novel bacterial and viral infections. The latest systems combine isothermal amplification with Cas-mediated detection, surpassing standard PCR methods in detection time and sensitivity, crucial for rapid diagnostics.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Dmitriy V. Semenov, Natalia S. Vasileva, Maya A. Dymova, Sergey V. Mishinov, Yulya I. Savinovskaya, Alisa B. Ageenko, Anton S. Dome, Nikita D. Zinchenko, Grigory A. Stepanov, Galina V. Kochneva, Vladimir A. Richter, Elena V. Kuligina
Summary: The study investigated the effects of VV-GMCSF-Lact on human glioma cells and non-malignant brain cells, revealing different gene expression patterns and pathways involved in cell sensitivity and cytotoxicity. The findings provide valuable insights into the mechanisms and influencing factors of oncolytic virotherapy.
Article
Biochemistry & Molecular Biology
Anastasiya Matveeva, Alexander Ryabchenko, Viktoria Petrova, Daria Prokhorova, Evgenii Zhuravlev, Alexander Zakabunin, Artem Tikunov, Grigory Stepanov
Summary: Research on Cas9 nucleases from different organisms has the potential to advance genome engineering and gene therapy tools. Thermophilic Cas9 nucleases, which have varying activity temperature ranges and PAM preferences, are expanding. This study focuses on constructing a compact Cas9 nuclease that displays nuclease activity in the 37-60 degrees C range.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Daria Prokhorova, Anastasiya Matveeva, Alexander Zakabunin, Alexander Ryabchenko, Grigory Stepanov
Summary: This study investigates the effect of N1-methylpseudouridine on the functioning of CRISPR/Cas9. The study shows that the presence of m1 psi in guide RNAs can help preserve on-target genome editing while significantly reducing the off-target effects of CRISPR/Cas9.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maya A. Dymova, Natalia S. Vasileva, Elena Kuligina, Yulya Savinovskaya, Nikita D. Zinchenko, Alisa B. Ageenko, Sergey Mishinov, Grigory A. Stepanov, Vladimir A. Richter, Dmitry Semenov
Summary: The formation of neurospheres in GBM cells is accompanied by changes in the expression of miRNAs and their target mRNAs. Upregulated miRNAs are associated with decreased levels of their target mRNAs, while downregulated miRNAs are associated with increased levels of their target mRNAs. These miRNAs and their target mRNAs may play important roles in the regulation of malignant growth and invasion in GBM.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.