Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application
出版年份 2020 全文链接
标题
Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application
作者
关键词
-
出版物
MOLECULES
Volume 25, Issue 15, Pages 3482
出版商
MDPI AG
发表日期
2020-08-03
DOI
10.3390/molecules25153482
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- A Near-Infrared Peptide Probe with Tumor-Specific Excretion-Retarded Effect for Image-Guided Surgery of Renal Cell Carcinoma
- (2020) Hong-Wei An et al. ACS Nano
- Development of elastin-like polypeptide for targeted specific gene delivery in vivo
- (2020) Aena Yi et al. JOURNAL OF NANOBIOTECHNOLOGY
- Cell Penetrating Peptides, Novel Vectors for Gene Therapy
- (2020) Rebecca E. Taylor et al. Pharmaceutics
- Nanoscale Self-Assembly for Therapeutic Delivery
- (2020) Santosh Yadav et al. Frontiers in Bioengineering and Biotechnology
- Near-Infrared Fluorescent and Magnetic Resonance Dual-Imaging Coacervate Nanoprobes for Trypsin Mapping and Targeted Payload Delivery of Malignant Tumors
- (2020) Heze Guo et al. ACS Applied Materials & Interfaces
- Self-Assembly of Amphiphilic Peptides for Recognizing High Furin-Expressing Cancer Cells
- (2019) Xiang Li et al. ACS Applied Materials & Interfaces
- Transfection of autologous host cells in vivo using gene activated collagen scaffolds incorporating star-polypeptides
- (2019) David P. Walsh et al. JOURNAL OF CONTROLLED RELEASE
- Cationic micelle: A promising nanocarrier for gene delivery with high transfection efficiency
- (2019) Haili Wang et al. JOURNAL OF GENE MEDICINE
- Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly
- (2019) Runqi Yan et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Heterochiral Assembly of Amphiphilic Peptides Inside the Mitochondria for Supramolecular Cancer Therapeutics
- (2019) M. T. Jeena et al. ACS Nano
- Self-Assembled Protein- and Peptide-Based Nanomaterials
- (2019) Priya Katyal et al. ACS Biomaterials-Science & Engineering
- Hierarchically oriented organization in supramolecular peptide crystals
- (2019) Chengqian Yuan et al. Nature Reviews Chemistry
- pH-Triggered Peptide Self-Assembly for Targeting Imaging and Therapy toward Angiogenesis with Enhanced Signals
- (2018) Yixia Qian et al. ACS Applied Materials & Interfaces
- Self-Assembled Peptide-Based Nanomaterials for Biomedical Imaging and Therapy
- (2018) Guo-Bin Qi et al. ADVANCED MATERIALS
- Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications
- (2018) Christopher D. Spicer et al. CHEMICAL SOCIETY REVIEWS
- Peptide-based nanoprobes for molecular imaging and disease diagnostics
- (2018) Pengcheng Zhang et al. CHEMICAL SOCIETY REVIEWS
- DNA vaccination for cervical cancer: Strategic optimisation of RALA mediated gene delivery from a biodegradable microneedle system
- (2018) Grace Cole et al. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
- Fabrication of cationic nanostructures from short self-assembling amphiphilic mixed α/β-pentapeptide: Potential candidates for drug delivery, gene delivery, and antimicrobial applications
- (2018) Rahul Goel et al. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- ε-Poly- l -Lysine/plasmid DNA nanoplexes for efficient gene delivery in vivo
- (2018) Haimanti Mandal et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS
- An efficient PEGylated gene delivery system with improved targeting: Synergism between octaarginine and a fusogenic peptide
- (2018) Ikramy A. Khalil et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS
- PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy
- (2018) Gizem Osman et al. JOURNAL OF CONTROLLED RELEASE
- Design, Synthesis, and Evaluation of Homochiral Peptides Containing Arginine and Histidine as Molecular Transporters
- (2018) Naglaa El-Sayed et al. MOLECULES
- Systematic study of the structural parameters affecting the self-assembly of cyclic peptide–poly(ethylene glycol) conjugates
- (2018) Edward D. H. Mansfield et al. Soft Matter
- Near infrared fluorescent peptide nanoparticles for enhancing esophageal cancer therapeutic efficacy
- (2018) Zhen Fan et al. Nature Communications
- Self-assembling asymmetric peptide-dendrimer micelles – a platform for effective and versatile in vitro nucleic acid delivery
- (2018) Ganesh R. Kokil et al. Scientific Reports
- Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties
- (2018) Vanessa Bellat et al. ADVANCED FUNCTIONAL MATERIALS
- Tumor-triggered transformation of chimeric peptide for dual-stage-amplified magnetic resonance imaging and precise photodynamic therapy
- (2018) Jin Zhang et al. BIOMATERIALS
- Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer
- (2018) Yijing Liu et al. CHEMICAL SOCIETY REVIEWS
- Applications of cyclic peptide nanotubes
- (2018) Wei-Hsien Hsieh et al. JOURNAL OF FOOD AND DRUG ANALYSIS
- Systemic siRNA delivery to tumors by cell-penetrating α-helical polypeptide-based metastable nanoparticles
- (2018) Yang Liu et al. Nanoscale
- Stimuli-Responsive Polypeptides for Biomedical Applications
- (2018) DaeYong Lee et al. Polymers
- Targeting Peptide‐Based Probes for Molecular Imaging and Diagnosis
- (2018) Weizhi Wang et al. ADVANCED MATERIALS
- Secondary Self-Assembly of Supramolecular Nanotubes into Tubisomes and Their Activity on Cells
- (2018) Johannes C. Brendel et al. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Cell-Penetrating Peptides Transport Noncovalently Linked Thermally Activated Delayed Fluorescence Nanoparticles for Time-Resolved Luminescence Imaging
- (2018) Zece Zhu et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Nanoribbons self-assembled from short peptides demonstrate the formation of polar zippers between β-sheets
- (2018) Meng Wang et al. Nature Communications
- Extracellular vesicles for nucleic acid delivery: progress and prospects for safe RNA-based gene therapy
- (2017) L Jiang et al. GENE THERAPY
- Polyethylene glycol–poly(ε-benzyloxycarbonyl-L-lysine)-conjugated VEGF siRNA for antiangiogenic gene therapy in hepatocellular carcinoma
- (2017) Gangmin Wang et al. International Journal of Nanomedicine
- Amelioration of atherosclerotic inflammation and plaques via endothelial adrenoceptor-targeted eNOS gene delivery using redox-sensitive polymer bearing l -arginine
- (2017) Qurrat Ul Ain et al. JOURNAL OF CONTROLLED RELEASE
- PEGylation rate influences peptide-based nanoparticles mediated siRNA delivery in vitro and in vivo
- (2017) Gudrun Aldrian et al. JOURNAL OF CONTROLLED RELEASE
- Bioreducible branched poly(modified nona-arginine) cell-penetrating peptide as a novel gene delivery platform
- (2017) Jisang Yoo et al. JOURNAL OF CONTROLLED RELEASE
- Peptide Self-Assembled Nanostructures for Drug Delivery Applications
- (2017) Taotao Fan et al. Journal of Nanomaterials
- Biological Photothermal Nanodots Based on Self-Assembly of Peptide–Porphyrin Conjugates for Antitumor Therapy
- (2017) Qianli Zou et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- DNA vaccination for cervical cancer; a novel technology platform of RALA mediated gene delivery via polymeric microneedles
- (2017) Ahlam A. Ali et al. Nanomedicine-Nanotechnology Biology and Medicine
- Self-assembly of bioactive peptides, peptide conjugates, and peptide mimetic materials
- (2017) Charlotte J. C. Edwards-Gayle et al. ORGANIC & BIOMOLECULAR CHEMISTRY
- Simultaneous silencing of TGF-β1 and COX-2 reduces human skin hypertrophic scar through activation of fibroblast apoptosis
- (2017) Jia Zhou et al. Oncotarget
- Self-assembly of peptide amphiphiles for drug delivery: the role of peptide primary and secondary structures
- (2017) Zhenhua Song et al. Biomaterials Science
- Histidine-enriched multifunctional peptide vectors with enhanced cellular uptake and endosomal escape for gene delivery
- (2017) Zhao Meng et al. Journal of Materials Chemistry B
- Recent Advances in Nanomaterials for Gene Delivery—A Review
- (2017) Michael Riley et al. Nanomaterials
- Bioreducible Fluorinated Peptide Dendrimers Capable of Circumventing Various Physiological Barriers for Highly Efficient and Safe Gene Delivery
- (2016) Xiaojun Cai et al. ACS Applied Materials & Interfaces
- Suppression of Hepatic Inflammation via Systemic siRNA Delivery by Membrane-Disruptive and Endosomolytic Helical Polypeptide Hybrid Nanoparticles
- (2016) Hua He et al. ACS Nano
- Stimuli-Responsive Codelivery of Oligonucleotides and Drugs by Self-Assembled Peptide Nanoparticles
- (2016) Severin J. Sigg et al. BIOMACROMOLECULES
- One-Dimensional Supramolecular Nanoplatforms for Theranostics Based on Co-Assembly of Peptide Amphiphiles
- (2016) Inhye Kim et al. BIOMACROMOLECULES
- Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights
- (2016) Jo-Ann Chuah et al. BIOMACROMOLECULES
- Development of self-assembling peptide nanovesicle with bilayers for enhanced EGFR-targeted drug and gene delivery
- (2016) Xiaofei Liang et al. BIOMATERIALS
- Peptide self-assembly: thermodynamics and kinetics
- (2016) Juan Wang et al. CHEMICAL SOCIETY REVIEWS
- Reducible self-assembling cationic polypeptide-based micelles mediate co-delivery of doxorubicin and microRNA-34a for androgen-independent prostate cancer therapy
- (2016) Chong Yao et al. JOURNAL OF CONTROLLED RELEASE
- Self-assembled peptide-based nanostructures: Smart nanomaterials toward targeted drug delivery
- (2016) Neda Habibi et al. Nano Today
- Transmembrane delivery of anticancer drugs through self-assembly of cyclic peptide nanotubes
- (2016) Jian Chen et al. Nanoscale
- Tunable Length of Cyclic Peptide–Polymer Conjugate Self-Assemblies in Water
- (2016) Sylvain Catrouillet et al. ACS Macro Letters
- Harnessing the PEG-cleavable strategy to balance cytotoxicity, intracellular release and the therapeutic effect of dendrigraft poly-l-lysine for cancer gene therapy
- (2016) Min Tang et al. Journal of Materials Chemistry B
- Deposition of gadolinium onto the shell structure of micelles for integrated magnetic resonance imaging and robust drug delivery systems
- (2016) Junping Ma et al. Journal of Materials Chemistry B
- Clinical development of gene therapy: results and lessons from recent successes
- (2016) Sandeep RP Kumar et al. Molecular Therapy-Methods & Clinical Development
- Positron Emission Tomography Imaging Using Radiolabeled Inorganic Nanomaterials
- (2015) Xiaolian Sun et al. ACCOUNTS OF CHEMICAL RESEARCH
- Peptide Nanofiber Complexes with siRNA for Deep Brain Gene Silencing by Stereotactic Neurosurgery
- (2015) Mariarosa Mazza et al. ACS Nano
- In Situ Formation of Nanofibers from Purpurin18-Peptide Conjugates and the Assembly Induced Retention Effect in Tumor Sites
- (2015) Di Zhang et al. ADVANCED MATERIALS
- Biodegradable Stearylated Peptide with Internal Disulfide Bonds for Efficient Delivery of siRNA In Vitro and In Vivo
- (2015) Zongguang Tai et al. BIOMACROMOLECULES
- Peptide self-assembly for nanomaterials: the old new kid on the block
- (2015) Emiliana De Santis et al. CHEMICAL SOCIETY REVIEWS
- Progress in Self-assembling Peptide-based Nanomaterials for Biomedical Applications
- (2015) Cui-Yun Yu et al. CURRENT TOPICS IN MEDICINAL CHEMISTRY
- Recent advances in dendrimer-based nanovectors for tumor-targeted drug and gene delivery
- (2015) Prashant Kesharwani et al. DRUG DISCOVERY TODAY
- Branched Amphiphilic Cationic Oligopeptides Form Peptiplexes with DNA: A Study of Their Biophysical Properties and Transfection Efficiency
- (2015) L. Adriana Avila et al. MOLECULAR PHARMACEUTICS
- RALA-mediated delivery of FKBPL nucleic acid therapeutics
- (2015) Rachel Bennett et al. Nanomedicine
- Self-Assembling Multifunctional Peptide Dimers for Gene Delivery Systems
- (2015) Kitae Ryu et al. Advances in Materials Science and Engineering
- Abstract LB-103: L1CAM-targeted delivery of siRNA using elastin-like polypeptide (ELP) nanoparticles inhibits the growth of human tumors implanted in mice
- (2015) Thomas Primiano et al. CANCER RESEARCH
- Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
- (2015) Jongsu Woo et al. PLoS One
- Cleavable PEGylation and Hydrophobic Histidylation of Polylysine for siRNA Delivery and Tumor Gene Therapy
- (2014) Haiyan Zhu et al. ACS Applied Materials & Interfaces
- PEG–Peptide Conjugates
- (2014) Ian W. Hamley BIOMACROMOLECULES
- Structural and functional photoacoustic molecular tomography aided by emerging contrast agents
- (2014) Liming Nie et al. CHEMICAL SOCIETY REVIEWS
- Cell-penetrating peptides: A tool for effective delivery in gene-targeted therapies
- (2014) Inês Rego de Figueiredo et al. IUBMB LIFE
- Self-assembling amphiphilic peptides
- (2014) Ashkan Dehsorkhi et al. JOURNAL OF PEPTIDE SCIENCE
- Elastin-Like Polypeptide Based Nanoparticles: Design Rationale Toward Nanomedicine
- (2014) Ferdinanda C. M. Smits et al. MACROMOLECULAR BIOSCIENCE
- Peptide vectors for gene delivery: from single peptides to multifunctional peptide nanocarriers
- (2014) Markus de Raad et al. Nanomedicine
- Self-assembly of peptides to nanostructures
- (2014) Dindyal Mandal et al. ORGANIC & BIOMOLECULAR CHEMISTRY
- Short peptide based self-assembled nanostructures: implications in drug delivery and tissue engineering
- (2014) Jiban Jyoti Panda et al. Polymer Chemistry
- Clinical translation of an ultrasmall inorganic optical-PET imaging nanoparticle probe
- (2014) E. Phillips et al. Science Translational Medicine
- Preparation and development of block copolypeptide vesicles and hydrogels for biological and medical applications
- (2014) Timothy J. Deming Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology
- Hybrid Polypeptide Micelles Loading Indocyanine Green for Tumor Imaging and Photothermal Effect Study
- (2013) Lei Wu et al. BIOMACROMOLECULES
- Synergistic gene and drug tumor therapy using a chimeric peptide
- (2013) Kai Han et al. BIOMATERIALS
- Fibrin-binding, peptide amphiphile micelles for targeting glioblastoma
- (2013) Eun Ji Chung et al. BIOMATERIALS
- Water-Soluble and pH-Responsive Polymeric Nanotubes from Cyclic Peptide Templates
- (2013) Robert Chapman et al. CHEMISTRY-A EUROPEAN JOURNAL
- Combined delivery of BCNU and VEGF siRNA using amphiphilic peptides for glioblastoma
- (2013) Na Yi et al. JOURNAL OF DRUG TARGETING
- Peptide-based targeting strategies for simultaneous imaging and therapy with nanovectors
- (2013) Antonella Accardo et al. POLYMER JOURNAL
- Synthetically designed peptide-based biomaterials with stimuli-responsive and membrane-active properties for biomedical applications
- (2013) Shirley Wong et al. Journal of Materials Chemistry B
- Cooperative Hierarchical Self-Assembly of Peptide Dendrimers and Linear Polypeptides into Nanoarchitectures Mimicking Viral Capsids
- (2012) Xianghui Xu et al. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Arginine functionalized peptide dendrimers as potential gene delivery vehicles
- (2012) Kui Luo et al. BIOMATERIALS
- Selective Gene Delivery to Cancer Cells Using an Integrated Cationic Amphiphilic Peptide
- (2012) Qiong Tang et al. LANGMUIR
- Direct formation of cationic polypeptide vesicle as potential carrier for drug and gene
- (2012) Jianxun Ding et al. MATERIALS LETTERS
- Oral Gene Delivery with cyclo-(d-Trp-Tyr) Peptide Nanotubes
- (2012) Wei-Hsien Hsieh et al. MOLECULAR PHARMACEUTICS
- Peptide Nanovesicles Formed by the Self-Assembly of Branched Amphiphilic Peptides
- (2012) Sushanth Gudlur et al. PLoS One
- Suicide gene therapy using reducible poly (oligo-d-arginine) for the treatment of spinal cord tumors
- (2011) Young-Wook Won et al. BIOMATERIALS
- Multifunctional nanoparticles for multimodal imaging and theragnosis
- (2011) Dong-Eun Lee et al. CHEMICAL SOCIETY REVIEWS
- Self-Assembly of Short Peptide Amphiphiles: The Cooperative Effect of Hydrophobic Interaction and Hydrogen Bonding
- (2011) Shuyi Han et al. CHEMISTRY-A EUROPEAN JOURNAL
- Stability of peptides and therapeutic success in cancer
- (2011) Marlène Pernot et al. Expert Opinion on Drug Metabolism & Toxicology
- Gene transfer by histidylated lipopolyplexes: A dehydration method allowing preservation of their physicochemical parameters and transfection efficiency
- (2011) Federico Perche et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS
- Uptake mechanisms of non-viral gene delivery
- (2011) Shengnan Xiang et al. JOURNAL OF CONTROLLED RELEASE
- Processable Cyclic Peptide Nanotubes with Tunable Interiors
- (2011) Rami Hourani et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- A Peptide-based Vector for Efficient Gene Transfer In Vitro and In Vivo
- (2011) Taavi Lehto et al. MOLECULAR THERAPY
- Enhancement of dendritic cells transfection in vivo and of vaccination against B16F10 melanoma with mannosylated histidylated lipopolyplexes loaded with tumor antigen messenger RNA
- (2011) Federico Perche et al. Nanomedicine-Nanotechnology Biology and Medicine
- Design of a peptide-based vector, PepFect6, for efficient delivery of siRNA in cell culture and systemically in vivo
- (2011) Samir EL Andaloussi et al. NUCLEIC ACIDS RESEARCH
- Exploiting Dimerization of Purely Peptidic Amphiphiles to Form Vesicles
- (2011) Thomas B. Schuster et al. Small
- Peptide nanotubes: molecular organisations, self-assembly mechanisms and applications
- (2011) Céline Valéry et al. Soft Matter
- Cell penetrating elastin-like polypeptides for therapeutic peptide delivery
- (2010) Gene L. Bidwell et al. ADVANCED DRUG DELIVERY REVIEWS
- Peptide-Based Probes for Targeted Molecular Imaging
- (2010) Seulki Lee et al. BIOCHEMISTRY
- Therapeutic effects of a reducible poly (oligo-d-arginine) carrier with the heme oxygenase-1 gene in the treatment of hypoxic-ischemic brain injury
- (2010) Hyesun Hyun et al. BIOMATERIALS
- Analysis of the Relationship between the Molecular Weight and Transfection Efficiency/Cytotoxicity of Poly-L-arginine on a Mammalian Cell Line
- (2010) BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Molecular self-assembly and applications of designer peptide amphiphiles
- (2010) Xiubo Zhao et al. CHEMICAL SOCIETY REVIEWS
- Endosomal escape pathways for delivery of biologicals
- (2010) Amir K. Varkouhi et al. JOURNAL OF CONTROLLED RELEASE
- Selective gene delivery in dendritic cells with mannosylated and histidylated lipopolyplexes
- (2010) Federico Perche et al. JOURNAL OF DRUG TARGETING
- Stimulus-responsive macromolecules and nanoparticles for cancer drug delivery
- (2010) Sarah R MacEwan et al. Nanomedicine
- Lipid-Shelled Vehicles: Engineering for Ultrasound Molecular Imaging and Drug Delivery
- (2009) Katherine W. Ferrara et al. ACCOUNTS OF CHEMICAL RESEARCH
- Peptide-mediated DNA condensation for non-viral gene therapy
- (2009) Paolo Saccardo et al. BIOTECHNOLOGY ADVANCES
- Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers
- (2009) Patrick Midoux et al. BRITISH JOURNAL OF PHARMACOLOGY
- Responsive magnetic resonance imaging contrast agents as chemical sensors for metals in biology and medicine
- (2009) Emily L. Que et al. CHEMICAL SOCIETY REVIEWS
- Reducible Poly(oligo-D-arginine) for Enhanced Gene Expression in Mouse Lung by Intratracheal Injection
- (2009) Young-Wook Won et al. MOLECULAR THERAPY
- Knocking down barriers: advances in siRNA delivery
- (2009) Kathryn A. Whitehead et al. NATURE REVIEWS DRUG DISCOVERY
- Targeting atherosclerosis by using modular, multifunctional micelles
- (2009) D. Peters et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Multifunctional imaging nanoprobes
- (2009) Peter A. Jarzyna et al. Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology
- Self-assembled Cationic Peptide Nanoparticles Capable of Inducing Efficient Gene Expression In Vitro
- (2008) Nikken Wiradharma et al. ADVANCED FUNCTIONAL MATERIALS
- Cell penetrating peptide-modified pharmaceutical nanocarriers for intracellular drug and gene delivery
- (2008) Vladimir P. Torchilin BIOPOLYMERS
- Designing peptide based nanomaterials
- (2008) Rein V. Ulijn et al. CHEMICAL SOCIETY REVIEWS
- Polymer-Based Gene Delivery: A Current Review on the Uptake and Intracellular Trafficking of Polyplexes
- (2008) Patrick Midoux et al. CURRENT GENE THERAPY
- Targeting of albumin-embedded paclitaxel nanoparticles to tumors
- (2008) Priya Prakash Karmali et al. Nanomedicine-Nanotechnology Biology and Medicine
- DNA condensation by poly-l-lysine at the single molecule level: Role of DNA concentration and polymer length
- (2007) Anita Mann et al. JOURNAL OF CONTROLLED RELEASE
- Cell-penetrating Peptide for Enhanced Delivery of Nucleic Acids and Drugs to Ocular Tissues Including Retina and Cornea
- (2007) Leslie N Johnson et al. MOLECULAR THERAPY
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreAdd your recorded webinar
Do you already have a recorded webinar? Grow your audience and get more views by easily listing your recording on Peeref.
Upload Now