Article
Veterinary Sciences
Barbara Colitti, Francesco Bonfante, Santina Grazioli, Laura Anfossi, Chiara Nogarol, Daniela Scalas, Luigi Bertolotti, Alessio Bortolami, Matteo Pagliari, Giulia Pezzoni, Ana Moreno, Davide Lelli, Maria Silvia Gennero, Alessandro Dondo, Emiliana Brocchi, Sergio Rosati
Summary: This study identified the most antigenic region of the SARS-CoV-2 nucleoprotein and developed a highly specific double antigen-based ELISA for detecting COVID-19 infection in susceptible animals.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Immunology
Rosana Wing-Shan Poon, Brian Pui-Chun Chan, Wan-Mui Chan, Carol Ho-Yan Fong, Xiaojuan Zhang, Lu Lu, Lin-Lei Chen, Joy-Yan Lam, Vincent Chi-Chung Cheng, Samson S. Y. Wong, Kin-Hang Kok, Kwok-Yung Yuen, Kelvin Kai-Wang To
Summary: The SARS-CoV-2 Omicron variant has caused a major wave of COVID-19 in Hong Kong from January to May 2022. A seroprevalence study found that although most individuals had been vaccinated or infected after the fifth wave, children and older adults remained the most vulnerable groups. Targeted public health measures should be implemented to mitigate the healthcare consequences of future waves.
EMERGING MICROBES & INFECTIONS
(2022)
Review
Biochemical Research Methods
Angela McArdle, Kirstin E. Washington, Blandine Chazarin Orgel, Aleksandra Binek, Danica-Mae Manalo, Alejandro Rivas, Matthew Ayres, Rakhi Pandey, Connor Phebus, Koen Raedschelders, Justyna Fert-Bober, Jennifer E. Van Eyk
Summary: The study summarizes proteomic research on hallmark proteins associated with COVID-19, as well as the highly variable humoral response and increased risk of autoimmunity in the disease.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Medicine, General & Internal
Dan H. Barouch
Summary: This article reviews the protective effects of vaccination and prior infection on severe Covid-19, and proposes future research directions.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Multidisciplinary Sciences
Qin Liu, Qi Su, Fen Zhang, Hein M. Tun, Joyce Wing Yan Mak, Grace Chung-Yan Lui, Susanna So Shan Ng, Jessica Y. L. Ching, Amy Li, Wenqi Lu, Chenyu Liu, Chun Pan Cheung, David S. C. Hui, Paul K. S. Chan, Francis Ka Leung Chan, Siew C. Ng
Summary: By integrating clinical features and multi-omics data, the authors identified specific gut microbiome patterns associated with disease severity and development of post-acute COVID-19 syndrome. These findings highlight the potential utility of host phenotype and multi-kingdom microbiota profiling as a prognostic tool for patients with COVID-19.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tanja Paul, Stephan Ledderose, Harald Bartsch, Na Sun, Sarah Soliman, Bruno Maerkl, Viktoria Ruf, Jochen Herms, Marcel Stern, Oliver T. Keppler, Claire Delbridge, Susanna Mueller, Guido Piontek, Yuki Schneider Kimoto, Franziska Schreiber, Tracy Ann Williams, Jens Neumann, Thomas Knoesel, Heiko Schulz, Ria Spallek, Matthias Graw, Thomas Kirchner, Axel Walch, Martina Rudelius
Summary: This study reveals the tropism of SARS-CoV-2 to adrenal glands, leading to adrenalitis and severe damage. This can impact immune regulation and adrenal function, highlighting the importance of monitoring adrenal function in the treatment and recovery of COVID-19.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Wenyu Chen, Ming Yao, Miaomiao Chen, Zhao Ou, Qi Yang, Yanbin He, Ning Zhang, Min Deng, Yuqi Wu, Rongchang Chen, Xiaoli Tan, Ziqing Kong
Summary: This study utilized untargeted metabolomics analysis to characterize the metabolic alterations in COVID-19 patients with nucleic acid turning negative. The results showed dysregulation in amino acid metabolism and lipid metabolism, highlighting the need for specific post-treatment care to aid the recovery of COVID-19 patients.
FRONTIERS IN PHARMACOLOGY
(2022)
Editorial Material
Immunology
Rustom Antia, M. Elizabeth Halloran
Summary: The article discusses the basic concepts underlying the transition from an epidemic to an endemic state, as well as the implications of this transition for COVID-19.
Article
Genetics & Heredity
Jeany Delafiori, Rinaldo Focaccia Siciliano, Arthur Noin de Oliveira, Jose Carlos Nicolau, Geovana Manzan Sales, Talia Falcao Dalcoquio, Estela Natacha Brandt Busanello, Adriana Eguti, Diogo Noin de Oliveira, Adriadne Justi Bertolin, Luiz Augusto dos Santos, Rocio Salsoso, Fabiana G. Marcondes-Braga, Nelson Duran, Mauricio Wesley Perroud Jr, Ester Cerdeira Sabino, Leonardo Oliveira Reis, Wagner Jose Favaro, Rodrigo Ramos Catharino
Summary: In this study, skin imprints and plasma samples were analyzed to study the metabolomics of COVID-19. The results showed that plasma samples outperformed skin imprints in diagnosing COVID-19, while skin imprints were excellent for assessing disease severity. Specific lipid molecules were identified as discriminative biomarkers for identifying hospitalized patients through skin imprinting. This research confirms the potential utility of skin imprinting as a non-invasive sampling method for COVID-19 screening.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Biochemical Research Methods
Sandip Chavan, Kiran K. Mangalaparthi, Smrita Singh, Santosh Renuse, Patrick M. Vanderboom, Anil Kumar Madugundu, Rohit Budhraja, Kathrine McAulay, Thomas E. Grys, Andrew D. Rule, Mariam P. Alexander, John C. O'Horo, Andrew D. Badley, Akhilesh Pandey
Summary: This study utilized mass spectrometry to detect SARS-CoV-2 nucleocapsid protein directly from urine of COVID-19 patients, revealing decreased levels of renal proteins associated with normal kidney function in the urine of COVID-19 patients. These findings provide insights into the pathogenesis of COVID-19.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Virology
Juan Zhao, Ling Wang, Madison Schank, Xindi Dang, Zeyuan Lu, Dechao Cao, Sushant Khanal, Lam N Nguyen, Lam N T Nguyen, Jinyu Zhang, Yi Zhang, James L. Adkins, Evan M. Baird, Xiao Y. Wu, Shunbin Ning, Mohamed El Gazzar, Jonathan P. Moorman, Zhi Q. Yao
Summary: Research shows that COVID-19-recovered individuals exhibit specific T cell responses, potentially offering protection against reinfection for some exposed individuals and inducing protective immunity in unexposed individuals upon vaccination. There is a need for a multi-epitope strategy in COVID-19 vaccine design due to the low immunogenicity of SARS-CoV-2 S and N peptides at inducing robust T cell responses.
Article
Microbiology
Lucas Barbosa Oliveira, Victor Irungu Mwangi, Marco Aurelio Sartim, Jeany Delafiori, Geovana Manzan Sales, Arthur Noin de Oliveira, Estela Natacha Brandt Busanello, Fernando Fonseca de Almeida e Val, Mariana Simao Xavier, Fabio Trindade Costa, Djane Clarys Baia-da-Silva, Vanderson de Souza Sampaio, Marcus Vinicius Guimaraes de Lacerda, Wuelton Marcelo Monteiro, Rodrigo Ramos Catharino, Gisely Cardoso de Melo
Summary: This study used metabolomics to identify potential metabolic signatures for discriminating severe COVID-19 from non-severe cases. The results showed that metabolites from porphyrin and purine pathways were significantly elevated in the severe disease group, while elevated levels of cholesteryl ester in non-severe patients were consistent with differences in blood cholesterol components. Pathway analysis revealed the impact of COVID-19 on glycerophospholipid and porphyrin metabolism, particularly in the glycerophospholipid and linoleic acid metabolism pathways.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Kruthi Suvarna, Akanksha Salkar, Viswanthram Palanivel, Renuka Bankar, Nirjhar Banerjee, Medha Gayathri J. Pai, Alisha Srivastava, Avinash Singh, Harsh Khatri, Sachee Agrawal, Om Shrivastav, Jayanthi Shastri, Sanjeeva Srivastava
Summary: By utilizing proteomic and metabolomic analysis, dysregulated proteins, peptides, and metabolites were identified in severe COVID-19 patients, with alterations in complement and coagulation cascade, platelet aggregation, myeloid leukocyte activation pathway, and arginine metabolism pathways highlighted. The study suggests targeting leukocyte activation and arginine metabolism as potential therapeutic strategies for COVID-19.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Lisa Pagani, Clizia Chinello, Giulia Risca, Giulia Capitoli, Lucrezia Criscuolo, Andrea Lombardi, Riccardo Ungaro, Davide Mangioni, Isabella Piga, Antonio Muscatello, Francesco Blasi, Andrea Favalli, Martina Martinovic, Andrea Gori, Alessandra Bandera, Renata Grifantini, Fulvio Magni
Summary: By studying the plasma proteome of 43 COVID-19 patients, differentially expressed proteins related to the severity of infection were identified. Three proteins were found to be able to accurately distinguish different stages of infection. These proteins may be associated with the severity of the disease and the immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Marcia C. Castro, Sun Kim, Lorena Barberia, Ana Freitas Ribeiro, Susie Gurzenda, Karina Braga Ribeiro, Erin Abbott, Jeffrey Blossom, Beatriz Rache, Burton H. Singer
Summary: The study found that COVID-19 in Brazil is spreading rapidly across municipalities with distinct spatial and temporal patterns of clustering, trajectories, and speed. Analysis shows that inadequate policy measures have led to high transmission and mortality burdens. The current surge in cases and deaths, along with the circulation of concerning variants, highlights the need for government to strengthen prevention and control measures.
Article
Chemistry, Analytical
Claudio Iacobucci, Michael Goetze, Christine Piotrowski, Christian Arlt, Anne Rehkamp, Christian Ihling, Christoph Hage, Andrea Sinz
ANALYTICAL CHEMISTRY
(2018)
Review
Chemistry, Multidisciplinary
Andrea Sinz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Biochemical Research Methods
Claudio Iacobucci, Christine Piotrowski, Anne Rehkamp, Christian H. Ihling, Andrea Sinz
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Article
Chemistry, Analytical
Michael Goetze, Claudio Iacobucci, Christian H. Ihling, Andrea Sinz
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Christoph Hage, Claudio Iacobucci, Michael Goetze, Andrea Sinz
JOURNAL OF MASS SPECTROMETRY
(2020)
Article
Biochemical Research Methods
Christian H. Ihling, Patrizia Springorum, Claudio Iacobucci, Christoph Hage, Michael Goetze, Mathias Schaefer, Andrea Sinz
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Review
Biology
Marcell Lederer, Simon Mueller, Markus Glass, Nadine Bley, Christian Ihling, Andrea Sinz, Stefan Huettelmaier
Summary: RNA-binding proteins (RBPs) play crucial roles in post-transcriptional control of gene expression and cellular processes, especially in cancer where dysregulation of RBPs, such as the MEX3 family member MEX3A, can impact proliferation, migration, and tumorigenesis. The unique domain structure of MEX3 proteins allows them to modulate both RNA and protein fate, and their oncogenic potential is supported by their impact on tumor cell behaviors and growth.
Article
Chemistry, Analytical
Christian H. Ihling, Lolita Piersimoni, Marc Kipping, Andrea Sinz
Summary: The combination of XL-MS and ion mobility is effective for analyzing protein conformation and interactions, as demonstrated in this study using different protein systems. The method allows for the identification of unique cross-linking sites and protein-protein interactions, showcasing its potential for both single protein structure analysis and system-wide protein interaction studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Julia Dorner, Patricia Korn, Kai Gruhle, Daniel Ramsbeck, Vasil M. Garamus, Hauke Lilie, Annette Meister, Christian Schwieger, Christian Ihling, Andrea Sinz, Simon Drescher
Summary: This study examines the potential of diazirine-modified lipids for lipid/peptide interactions research, revealing unexpected backfolding behavior of the diazirine-containing stearoyl chain in the lipid. This finding challenges the application of DiazPC for future studies on peptide and protein/lipid interactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Nina Braun, Soren Friis, Christian Ihling, Andrea Sinz, Jacob Andersen, Stephan A. Pless
Summary: The study established a high-throughput protocol to investigate functional and pharmacological properties of ncAA-containing hASIC1a variants in mammalian cells using automated patch clamp. The approach was proven to be efficient and versatile, allowing for complex pharmacological modulation and insight into protein interactions. It is anticipated that this method can be applied to other ion channels for future studies in mammalian cells.
Review
Chemistry, Multidisciplinary
Lolita Piersimoni, Panagiotis L. Kastritis, Christian Arlt, Andrea Sinz
Summary: Mass spectrometry (MS) is a key technology in structural biology, and chemical cross-linking combined with mass spectrometry (XL-MS) plays an important role in studying protein structures and protein-protein interactions. This review outlines the contributions of XL-MS, including cross-linking reagents, software tools, workflows, and significant examples, in characterizing proteins and their interactions in vitro and in vivo. Computational modeling and integration with other structural biology techniques have greatly enhanced the capabilities of XL-MS.
Article
Multidisciplinary Sciences
Christian Arlt, Kerstin Nutschan, Alexander Haase, Christian Ihling, Dirk Taenzler, Andrea Sinz, R. Gary Sawers
Summary: The research shows that HybG plays a crucial role in the biosynthesis and delivery process of the hydrogenase catalytic subunit, suggesting its shuttle between the assembly complex and the apo-catalytic subunit. These findings provide new insights into how organometallic cofactors are assembled and transferred to their client proteins.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Dirk Taenzler, Marc Kipping, Marcell Lederer, Wiebke Guenther, Christian Arlt, Stefan Huettelmaier, Andreas Merkenschlager, Andrea Sinz
Summary: We demonstrated the effects of caffeine, theophylline, and istradefylline on cAMP production by ADCY5-overexpressing cell lines. cAMP levels were compared between ADCY5 wild-type and R418W mutant cells. The purine derivatives reduced ADCY5-catalyzed cAMP production, with the R418W mutant cells showing the most significant reduction. In a case study, a slow-release formulation of theophylline showed better improvement in symptoms of ADCY5-related dyskinesia compared to previously administered caffeine. Theophylline could be considered as an alternative therapeutic option for treating ADCY5-related dyskinesia in patients.
Article
Biochemical Research Methods
Christian Tueting, Lisa Schmidt, Ioannis Skalidis, Andrea Sinz, Panagiotis L. Kastritis
Summary: In this study, protein communities and inter-protein interactions were identified in a yeast native cell extract using proteomics analysis and cryogenic electron microscopy. By combining AI-guided protein structure prediction, a density was successfully assigned to the yeast fatty acid synthase complex.
