Article
Food Science & Technology
Jie Kang, Xiaohang Zhou, Wen Zhang, Fangyi Pei, Jingping Ge
Summary: This study found that the response of lactic acid bacteria to acetic acid stress led to an increase in the production rate and concentration of bacteriocin, as well as an increase in the number of differentially expressed genes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
X. Zhao, R. Hu, Y. Liu, Y. He, S. Li, J. Yang, J. Zhou, J. Zhang
Summary: By genome sequencing and analysis, it was found that Lactobacillus paracasei SLP 16 is a safe probiotic strain with coding genes related to acid tolerance and bile salt tolerance, no coding genes related to virulence factors, and resistance to certain antibiotics.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Yuhan Sun, Huanzi Wang, Lei Zhou, Mengjia Chang, Tianli Yue, Yahong Yuan, Yiheng Shi
Summary: This study characterized the distribution of selenium in the cell of the selenium-enriched lactic acid bacteria Lactobacillus Paracasei 20241, and found that the majority of selenium was organic selenium in the form of selenoprotein. This finding has significant implications for using this bacteria as a dietary supplement.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Liangzhi Li, Hetian Zhang, Delong Meng, Huaqun Yin
Summary: This study investigates the cellular response of L. paracasei B1 under high-density culture conditions and explores its response mechanism to environmental stress. Transcriptomics techniques are used to analyze the nutrient metabolism pattern and differentially expressed genes during growth. The findings provide insights into the functions of key genes and the metabolic pathways of the strain.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Yuwei Liu, Kemin Mao, Nan Zhang, Bimal Chitrakar, Pimiao Huang, Xianghong Wang, Bing Yang, Yaxin Sang
Summary: This study used gradient ethanol precipitation method to extract extracellular polysaccharides (VL8-EPS) from Lactobacillus paracasei VL8 and found that ethanol concentration affected their physicochemical properties and immunomodulatory effects. Among the different ethanol concentrations, VL8-EPS50 displayed better immunomodulatory activities, enhancing phagocytic activity and nitric oxide secretion.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Nutrition & Dietetics
Fang Wu, Bing Fang, Guna Wuri, Liang Zhao, Fudong Liu, Ming Zhang
Summary: The study found that Lactobacillus paracasei L9 and Bifidobacterium animalis A6 can alleviate the symptoms of periodontitis by regulating oral microbiota. They can inhibit inflammatory response, increase the expression of anti-inflammatory cytokines, reduce the proportion of pathogenic bacteria, and inhibit the levels of bacterial virulence factors related to periodontitis. These results suggest that A6 and L9 may be promising prophylactic and therapeutic agents for periodontitis.
Article
Biochemistry & Molecular Biology
Giuseppe Esposito, Marcella Pesce, Luisa Seguella, Jie Lu, Chiara Corpetti, Alessandro Del Re, Fatima Domenica Elisa De Palma, Giovanni Esposito, Walter Sanseverino, Giovanni Sarnelli
Summary: This study demonstrates that utilizing NAPE-PLD-expressing Lactobacillus paracasei F19 (pNAPE-LP) and ultra-low palmitate supply can enhance the delivery of PEA in the intestine, leading to significant improvements in clinical and histological damage scores in a murine model of ulcerative colitis. The coadministration of pNAPE-LP and palmitate results in reduced neutrophil infiltration, decreased levels of pro-inflammatory cytokines and oxidative stress markers, and improved epithelial barrier integrity, suggesting a potential new therapeutic approach for controlling intestinal inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhu Zeng, Yi Yang, Xinxin Zhong, Fangyin Dai, Shangwu Chen, Xiaoling Tong
Summary: This study investigated the beneficial effects of Lactobacillus paracasei strain L14 on type 2 diabetes mellitus (T2DM) rats. The preventive supplementation of strain L14 showed excellent anti-diabetic effects, reducing hyperglycemia, protecting pancreatic beta-cell and liver function, and improving dyslipidemia and inflammation. The strain also modulated the gut microbiota to alleviate gut dysbiosis. The whole-genome sequencing of L14 identified genes related to antioxidant systems and other beneficial microbial metabolites, highlighting its potential applications in functional food and pharmaceutical industries.
Article
Spectroscopy
Ling Zong, Chao Li, Yang Zhong, Jie Shi, Zhanyuan Yuan, Xin Wang
Summary: Recent studies have shown that cisplatin may induce apoptosis in beneficial bacteria, highlighting the importance of exploring the damage mechanism of cisplatin on beneficial bacteria.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Nutrition & Dietetics
Shumei Wang, Yi Shan, Shuang Zhang, Lanwei Zhang, Yuehua Jiao, Dijia Xue, Lili Zhang, Huaxi Yi
Summary: In this study, it was found that whole peptidoglycan (WPG) from L. paracasei subsp. paracasei X12, obtained from traditional cheese in northwestern China, inhibited proliferation and induced apoptosis in HT-29 cells in a dose-dependent manner. The induction of apoptosis by WPG was associated with mitochondrial dysfunction and ROS accumulation, as well as the activation of Caspase 3 and alterations in the expression of proapoptotic and antiapoptotic genes. These findings suggest that WPG could potentially be used as a natural agent for cancer treatment.
Article
Immunology
Jiayuan Cao, Qiqi Liu, Haining Hao, Yushan Bu, Xiaoying Tian, Ting Wang, Huaxi Yi
Summary: This study identified Lactobacillus paracasei X11 as a probiotic strain with uric acid-lowering activity. It effectively degraded nucleotides and nucleosides, regulated uric acid metabolism-related factors, and restored the ratio of intestinal microbiota in a mouse model of hyperuricemia. This suggests that L. paracasei X11 has the potential to be developed as a novel probiotic strain for the amelioration of hyperuricemia.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yong Jun Goh, Rodolphe Barrangou
Summary: This study developed a CRISPR-based editing system for genome manipulations in three Lactobacillus species, demonstrating its efficacy in two distantly related Lactobacillus species. This versatile Cas9 system facilitates genome engineering compared to conventional gene replacement systems and is a valuable gene editing modality in species without native CRISPR-Cas systems. Overall, this portable tool expands the genome editing toolbox for studying health-promoting mechanisms and engineering beneficial microbes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Qingqing Ma, Zhen Jin, Yue Xiao, Jinlin Zhu, Wenwei Lu, Wei Chen, Hongchao Wang, Xiuwen Zhou
Summary: L. rhamnosus FXJWS19L2 and L. paracasei FQHXN23L6 exhibited high tetracycline resistance without containing known tetracycline resistance genes. Transcriptomic analysis revealed that the differentially expressed genes (DEGs) in these strains were mainly involved in ribosomal protein synthesis, ABC transporters, and metabolism of various substances. Overexpression of the most significantly up-regulated gene (yjcA), encoding an ABC transporter, improved the resistance of bacteria to tetracycline. This study provides insights into the resistance mechanism of these strains and offers a theoretical reference for the safe application of such lactic acid bacteria in food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Hua Jiang, Shengjie Tan, Ke Ning, Hao Li, Wenzhi Zhao, Ai Zhao, Hong Zhu, Shijie Wang, Peiyu Wang, Yumei Zhang
Summary: In this study, intervention with the probiotic LP N1115 in dyslipidemia patients resulted in lower levels of serum total cholesterol and apolipoprotein B, as well as improvements in glucose metabolism. Probiotic intervention also led to changes in certain components and diversity indices of the gut microbiota.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Food Science & Technology
Pengxin Ye, Jiawen Wang, Manman Liu, Ping Li, Qing Gu
Summary: A novel bacteriocin named ZFM54, produced by Lactobacillus paracasei ZFM54, was isolated from newborn infant feces, possessing high thermal stability and broad-spectrum antimicrobial activity, making it a potential biological preservative in the food industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)