Article
Biochemical Research Methods
Chunyan Niu, Xia Wang, Yongzhuo Zhang, Lin Lu, Di Wang, Yunhua Gao, Shangjun Wang, Jingyan Luo, Ying Jiang, Nuo Wang, Yong Guo, Lingxiang Zhu, Lianhua Dong
Summary: The COVID-19 pandemic has caused serious harm to global health. RT-dPCR has shown good consistency and comparability in quantifying SARS-CoV-2 RNA, demonstrating its potential for virus detection and testing capabilities in participating laboratories.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Marco Favaro, Walter Mattina, Enrico Salvatore Pistoia, Roberta Gaziano, Paolo Di Francesco, Simon Middleton, Silvia D'Angelo, Tullio Altarozzi, Carla Fontana
Summary: A new qualitative RT-PCR probe has been developed by the research team for detecting COVID-19 infections, with a coincidence rate of 0.96 and LoD of 140cp/reaction for N and 14cp/reaction for RdRp and E. The kit is designed to be open and can be distributed in large quantities to any microbiological laboratory worldwide.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jean-Baptiste Lascarrou, Gwenhael Colin, Aurelie Le Thuaut, Nicolas Serck, Mickael Ohana, Bertrand Sauneuf, Guillaume Geri, Jean-Baptiste Mesland, Gaetane Ribeyre, Claire Hussenet, Anne Sophie Boureau, Thomas Gille
Summary: In a multicenter case-control study on COVID-19 patients, it was found that patients with symptoms of headache and fatigue/malaise were more likely to have a false-negative result in their first RT-PCR test. Conversely, patients with elevated platelet and C-reactive protein levels were at a higher risk of a false-negative first RT-PCR test result. This highlights the importance of evaluating strategies involving serial RT-PCR testing for COVID-19 diagnosis.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Angie L. Ramirez, Nicolas Luna, Luz H. Patino, Sergio Castaneda, Marina Munoz, Nathalia Ballesteros, Julie Perez, Camilo A. Correa-Cardenas, Maria Clara Duque, Claudia Mendez, Carolina Oliveros, Alberto E. Paniz-Mondolfi, Juan David Ramirez
Summary: This study assessed the circulation of SARS-CoV-2 variants among vaccinated military personnel in Bogota, Colombia. The Mu lineage (B.1.621 and B.1.621.1) was found to be the most frequent infecting lineage in vaccinated individuals, possibly due to specific mutations conferring it with vaccine-induced immune escape ability. The findings highlight the importance of genomic tracking and epidemiological surveillance in studying emerging variants and their impact on global vaccination efforts.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Mariana Fernandez-Pittol, Juan Carlos Hurtado, Maryam Ali, Alvar Simarro, Fadiana Proano, Maria Sierra, Jordi Vila
Summary: In various healthcare settings, such as emergency surgery patients, there is a need for rapid detection of SARS-CoV-2. The QuantuMDx Q-POC is an automated fast workflow platform that detects three genes, allowing differentiation between SARS-CoV-2 and other coronaviruses. This study compared QuantuMDx Q-POC with the Cobas 6800 algorithm for SARS-CoV-2 detection, showing high sensitivity and a short detection time of 30 minutes. Overall, the QuantuMDx Q-POC system is a reliable option for rapid SARS-CoV-2 detection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Hugo Alberto Barrera Saldana, Carolina Rivera Santiago, Raul Rodriguez Palacios
Summary: COVID-19 was first detected in Mexico in February 2020. Despite the initial lack of recognition by health authorities, we anticipated the need for viral detection tests and established a rapid molecular diagnostic service for SARS-CoV-2. Standardized protocols for biospecimen sampling, transportation, biobanking, preanalytical validation, and nucleic acids testing were implemented. The adoption of automation processes, a quality control/quality assurance system, and close collaboration with health authorities were key factors in our success.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Cristina Garcia-Salguero, Esther Culebras, Paloma Merino, Elvira Baos, Alberto Delgado-Iribarren
Summary: The study evaluated the use of antigen test samples for RT-PCR analysis and found that a single sample can be used for both tests, reducing unnecessary management of suspected individuals and improving staff and patient safety and comfort.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Infectious Diseases
Ming Liao, Jianmin Wu, Manman Dai, Huanan Li, Nan Yan, Runyu Yuan, Chungen Pan
Summary: A convenient and safe method for rapid detection of SARS-CoV-2 in samples was developed, which can be used to identify animals susceptible to infection and determine the virus status in samples, as well as for developing assays for detecting other viruses.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Sourav Dutta Dip, Shovon Lal Sarkar, Md. Ali Ahasan Setu, Prosanto Kumar Das, Md. Hasan Ali Pramanik, A. S. M. Rubayet Ul Alam, Hassan M. Al-Emran, M. Anwar Hossain, Iqbal Kabir Jahid
Summary: The efficiency of different RT-PCR kits and a SYBR-green method for detecting recent variants of SARS-CoV-2 was compared. Results showed that Maccura kit had 81.8% specificity and 82.5% sensitivity, A*Star kit had 100% specificity and 75.4% sensitivity, Da An Gene kit had 100% specificity and 68.4% sensitivity, Sansure kit had 54.5% specificity and 91.2% sensitivity, and TaqPath kit had 100% specificity and 70.2% sensitivity. The in-house SYBR-Green method had 90.9% specificity and 91.2% sensitivity. Kits targeting more genes showed better accuracy and reduced false positive results (< 20%).
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Torsten Hansen, Ulf Titze, Nidhi Su Ann Kulamadayil-Heidenreich, Sabine Glombitza, Johannes Josef Tebbe, Christoph Roecken, Birte Schulz, Michael Weise, Ludwig Wilkens
Summary: An 86-year-old man who was previously asymptomatic received the COVID-19 vaccine and tested positive for SARS-CoV-2 postmortem, showing immunogenicity without sterile immunity. Despite the cause of death being acute bronchopneumonia and tubular failure, characteristic morphological features of COVID-19 were not observed.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Daniel F. F. Escobar, Pablo Diaz, Diego Diaz-Dinamarca, Rodrigo Puentes, Pedro Alarcon, Barbara Alarcon, Ivan Rodriguez, Ricardo A. Manzo, Daniel A. Soto, Liliana Lamperti, Janepsy Diaz, Heriberto E. Garcia-Escorza, Abel E. Vasquez
Summary: The Chilean city of Concepcion experienced a second wave of COVID-19 in January and the whole country faced the same situation in early April 2021. A study conducted during total quarantine in Concepcion validated a highly sensitive and specific RT-PCR method using saliva specimens, showing comparable performance to NPS specimens. This modified method provides a real testing alternative to health authorities and local laboratories in Chile.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Medicine, General & Internal
Tingting Du, Zhongliang Wang
Summary: Since 2019, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global public health event. Real-time reverse transcription polymerase chain reaction (RT-PCR) tests of upper respiratory tract specimens have been commonly used for confirmation of SARS-CoV-2. A retrospective analysis of patients diagnosed with coronavirus disease 2019 in a hospital in Wuhan was conducted, focusing on the pattern of results from repeated RT-PCR tests. Out of 984 hospitalized patients, 36.8% showed positive results from repeated RT-PCR tests. However, consecutive negative RT-PCR tests did not guarantee viral clearance, as some patients tested positive even after multiple negative tests.
Article
Medicine, General & Internal
Adam Konka, Mateusz Lejawa, Jadwiga Gazdzicka, Aneta Bochenek, Martyna Fronczek, Joanna Katarzyna Strzelczyk
Summary: This study analyzed epidemiological data from a major laboratory in the Silesian voivodship of Poland and found that SARS-CoV-2 infections are more common in urban areas. There were discrepancies in the detection of SARS-CoV-2 variants in samples from potentially reinfected patients. The study highlights the higher risk of infection in the Upper Silesian population and the potential impact on the economy and healthcare in the region.
Article
Virology
Ekawat Pasomsub, Siriorn P. Watcharananan, Treewat Watthanachockchai, Kingkan Rakmanee, Boonrat Tassaneetrithep, Sasisopin Kiertiburanakul, Angsana Phuphuakrat
Summary: Saliva has been found to be a potential alternative specimen for detecting SARS-CoV-2, and pooling saliva samples for testing can help conserve healthcare resources without compromising the detection of the virus.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medicine, General & Internal
Yesit Bello-Lemus, Marco Anaya-Romero, Janni Gomez-Montoya, Moises Arquez, Henry J. Gonzalez-Torres, Elkin Navarro-Quiroz, Leonardo Pacheco-Londono, Lisandro Pacheco-Lugo, Antonio J. Acosta-Hoyos
Summary: Developing an efficient and cost-effective method for detecting SARS-CoV-2 is essential, especially in developing countries or settings with limited resources.
Review
Microbiology
Bo-Shiun Chen, Hou-Chen Lee, Kuo-Ming Lee, Yu-Nong Gong, Shin-Ru Shih
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Hsing- Huang, Jhao-Yin Lin, Hsiao-Chu Chiang, Pen-Nien Huang, Qing-Dong Lin, Shin-Ru Shih
JOURNAL OF INFECTIOUS DISEASES
(2020)
Review
Biochemistry & Molecular Biology
Yen-Chin Liu, Rei-Lin Kuo, Shin-Ru Shih
BIOMEDICAL JOURNAL
(2020)
Article
Microbiology
Sheng-Yu Huang, Yu-An Kung, Peng-Nien Huang, Sheng-Yun Chang, Yu-Nong Gong, Yi-Ju Han, Huan-Jung Chiang, Kuan-Ting Liu, Kuo-Ming Lee, Chia-Yu Chang, Chia-Ching Chang, Chung-Guei Huang, Shin-Ru Shih
Summary: The study reveals that SARS-CoV-2 remains stable and infectious after refrigeration or freezing, with stability and infectivity depending on the spike variant. The S-G614 variant shows higher stability and better binding ability with the ACE2 receptor, contributing to its rapid spread and infectivity. Further precautions should be taken when handling food stored in cold conditions.
Article
Virology
Jing-Yi Lin, Kuo-Feng Weng, Chih-Kuang Chang, Yu-Nong Gong, Guo-Jen Huang, Hui-Lan Lee, Yen-Cheng Chen, Chien-Chih Huang, Jia-Ying Lu, Peng-Nien Huang, Huan-Jung Chiang, Che-Min Chen, Shin-Ru Shih
Summary: This study presents an oral infection mouse model for EV-A71 that efficiently infects weaned mice and allows tracking of viral spread, contributing to research on viral pathogenesis and neuroinvasion. The model offers advantages over existing animal models in studying viral spread, tissue tropism, and viral pathogenesis.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Yu-An Kung, Huan-Jung Chiang, Mei-Ling Li, Yu-Nong Gong, Hsin-Ping Chiu, Chuan-Tien Hung, Peng-Nien Huang, Sheng-Yu Huang, Pei-Yu Wang, Tsu-An Hsu, Gary Brewer, Shin-Ru Shih
Summary: This study reveals the involvement of ACSL4 in enterovirus replication organelle formation and the induction of ferroptosis by enteroviruses and coronaviruses via ACSL4. Inhibition of ACSL4 using ferroptosis inhibitors reduces viral loads of enteroviruses and coronaviruses, suggesting the potential of ACSL4-mediated ferroptosis as a promising therapeutic target for viral diseases.
Article
Microbiology
Kuan-Ting Liu, Yu-Nong Gong, Chung-Guei Huang, Peng-Nien Huang, Kar-Yee Yu, Hou-Chen Lee, Sun-Che Lee, Huan-Jung Chiang, Yu-An Kung, Yueh-Te Lin, Mei-Jen Hsiao, Po-Wei Huang, Sheng-Yu Huang, Hsin-Tai Wu, Chih-Ching Wu, Rei-Lin Kuo, Kuan-Fu Chen, Chuan-Tien Hung, Kasopefoluwa Y. Oguntuyo, Christian S. Stevens, Shreyas Kowdle, Hsin-Ping Chiu, Benhur Lee, Guang-Wu Chen, Shin-Ru Shih
Summary: In this study, an enzyme-linked immunosorbent assay (ELISA) was developed to detect SARS-CoV-2-neutralizing antibodies in serum. The method accurately quantified neutralizing antibody titers and showed potential for assessing vaccine efficacy.
Review
Microbiology
Yu-An Kung, Kuo-Ming Lee, Huan-Jung Chiang, Sheng-Yu Huang, Chung-Jung Wu, Shin-Ru Shih
Summary: This review discusses the molecular mechanisms of SARS-CoV-2 and related coronaviruses, with a focus on host factors or pathways identified through genome-wide CRISPR screening that can be targeted for the development of effective antiviral agents.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Immunology
Guan-Hong Wu, Kuo-Ming Lee, Chia-Yu Kao, Shin-Ru Shih
Summary: The role of the internal ribosome entry site (IRES) in determining the neurovirulence of enteroviruses EV-A71 and CV-A16 was characterized using an oral inoculation model. Replacing EV-A71 IRES with CV-A16 abolished neuropathological phenotypes, indicating that IRES determines the neurotropic potential of these viruses. Tissue-specific replication can be reflected in cell-based characterization.
MICROBES AND INFECTION
(2023)
Article
Cell Biology
Yi-Yin Chen, Ching-Tai Huang, Shiao-Wen Li, Yi-Jiun Pan, Tzu-Lung Lin, Ya-Yu Huang, Ting-Hsuan Li, Yu-Ching Yang, Yu-Nong Gong, Yu-Chia Hsieh
Summary: The study revealed that IAV infection alters host metabolism to benefit pneumococcal fitness during coinfection, leading to higher susceptibility to pneumococcal infection. Modulation of host metabolism during IAV infection may serve as a potential therapeutic intervention against secondary bacterial infections caused by serotype 3 pneumococci during IAV outbreaks in the future.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Microbiology
Yu-An Kung, Huan-Jung Chiang, Mei-Ling Li, Yu-Nong Gong, Hsin-Ping Chiu, Chuan-Tien Hung, Peng-Nien Huang, Sheng-Yu Huang, Pei-Yu Wang, Tsu-An Hsu, Gary Brewer, Shin-Ru Shih
Summary: ACSL4 plays a key role in enterovirus replication organelle formation, both enteroviruses and coronaviruses can induce ferroptosis via ACSL4, inhibiting ACSL4 can reduce the replication of various viruses, suggesting ACSL4-mediated ferroptosis is a promising therapeutic target for viral diseases.