Article
Biology
Po Jui Chen, Anna B. McMullin, Bryan J. Visser, Qian Mei, Susan M. Rosenberg, David Bates
Summary: In bacteria, sister replisomes are functionally interdependent and remain colocalized throughout replication. Our study reveals the crucial role of physical association between sister replisomes in ensuring an efficient and uninterrupted replication program.
Article
Oncology
Hong-My Nguyen, Shreyas Gaikwad, Mariam Oladejo, Manas Yogendra Agrawal, Sanjay K. Srivastava, Laurence M. Wood
Summary: Type 1 interferons are central in regulating the immune system's response and induce interferon-stimulated genes (ISGs). Among them, ISG15 has been extensively studied for its role in cancer as both a promoter and inhibitor of tumor growth. This review provides an update on recent observations and findings related to ISG15 in cancer, including its molecular mechanisms and therapeutic applications.
Article
Biochemistry & Molecular Biology
Clemence Claussin, Jacob Vazquez, Iestyn Whitehouse
Summary: This study developed a method called Replicon-seq, which can accurately define the movement of individual replisomes across the genome. Using this method, the study found that sister replisomes function autonomously and progress through chromatin with remarkable consistency. Replication forks pause briefly when encountering obstacles but quickly resume synthesis, and the helicase Rrm3 plays a critical role in mitigating the effect of protein barriers and facilitating efficient termination.
Article
Biochemistry & Molecular Biology
Daniel J. Goodall, Katie H. Jameson, Michelle Hawkins, Christian J. Rudolph
Summary: The termination phase of DNA replication is complex, and in Escherichia coli, it involves the formation of a replication fork trap system facilitated by Tus protein binding to ter sites. While this system is present in some bacterial species, it is not a universal feature of bacterial chromosomes. The highly conserved ter sites in E. coli genomes, with slightly more variability in Shigella genomes, suggest the physiological significance of the replication fork trap system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Xiwa Zhao, Jingjing Wang, Yaojie Wang, Mengmeng Zhang, Wei Zhao, Hui Zhang, Lianmei Zhao
Summary: This study found elevated expression of ISG15 in endometrial carcinoma (EC), which was associated with poor clinical outcomes and pathological stage. ISG15 promoted EC progression by influencing cell cycle and activating the MYC signaling pathway. Additionally, high levels of ISG15 were associated with immune escape ability. These findings suggest that ISG15 may serve as a potential marker for diagnosis, prognosis, and therapeutic immunity in EC.
Article
Oncology
Vladimir Bezrookove, John M. Patino, Mehdi Nosrati, Pierre-Yves Desprez, Sean McAllister, Liliana Soroceanu, Ari Baron, Robert Osorio, Mohammed Kashani-Sabet, Altaf A. Dar
Summary: Cholangiocarcinoma is a rare and aggressive tumor with limited treatment options, but PARP inhibitors show promise as a novel therapy for CCA. Mutations in DNA damage response genes make these tumors susceptible to PARP inhibitors, which can suppress cancer cell viability and induce apoptosis.
Review
Biochemistry & Molecular Biology
Holly M. M. Radford, Casey J. J. Toft, Alanna E. E. Sorenson, Patrick M. M. Schaeffer
Summary: Over 1.2 million deaths are caused by multi-drug-resistant bacteria each year, as a result of their fast replication and rapid evolution. The accumulation of resistance genes in pathogens has made current antibiotic treatments ineffective, leading to a decrease in reliable treatment options for MDR-associated diseases. This review provides a critical evaluation of DNA replication initiation in bacteria and highlights the potential of essential initiation proteins as emerging drug targets. It also examines the available methods to study and screen promising replication initiation proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ilir I. Cinoku, Kleio-Maria Verrou, Evangelia Piperi, Michael Voulgarelis, Haralampos M. Moutsopoulos, Clio P. Mavragani
Summary: The study found that ISG-15 gene can serve as a potential biomarker to distinguish SS patients with and without lymphoma, showing predictive value for lymphoma development. Additionally, PB and LMSG tissues share a similar transcriptional profile in the type I IFN pathway.
JOURNAL OF AUTOIMMUNITY
(2021)
Review
Biochemistry & Molecular Biology
Thomas A. Guilliam
Summary: The eukaryotic replisome encounters obstacles such as damaged and structured DNA during replication, but various mechanisms exist to overcome these hurdles, maintain progression, and prevent fork collapse. Recent advances in structural, biochemical, and single-molecule approaches have enhanced our understanding of the replisome's architecture, response to impediments, and DNA damage tolerance mechanisms.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cell Biology
Jing Zhang, Hao Lian, Kui Chen, Ying Pang, Mu Chen, Bingsong Huang, Lei Zhu, Siyi Xu, Min Liu, Chunlong Zhong
Summary: The protective role of RECQ1 in DNA replication forks in GBM cells helps to maintain genomic stability and enhance replication stress resistance, providing new insights into overcoming therapy resistance in GBM.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu-Ting Hsieh, Tsung-Lin Tsai, Shen-Yan Huang, Jian-Wen Heng, Yu-Chia Huang, Pei-Yuan Tsai, Chia-Chun Tu, Tai-Ling Chao, Ya-Min Tsai, Pei-Ching Chang, Chien-Kuo Lee, Guann-Yi Yu, Sui-Yuan Chang, Ivan L. L. Dzhagalov, Chia-Lin Hsu
Summary: A growing body of evidence highlights the role of metabolic reprogramming in immune cells to combat infections. However, the regulation of metabolite transporters that support metabolic demands remains poorly understood. This study demonstrates that equilibrative nucleoside transporter 3 (ENT3) is part of the innate immune response and is upregulated upon pathogen invasion. The expression of ENT3 is directly regulated by type I interferon-induced signaling and is required for the entry and replication of several viruses, including SARS-CoV-2.
Article
Biochemistry & Molecular Biology
Swagata Halder, Lepakshi Ranjha, Angelo Taglialatela, Alberto Ciccia, Petr Cejka
Summary: SMARCAL1, ZRANB3 and HLTF are important factors in remodeling replication forks to maintain genome stability. RAD51 and the RAD51 paralog complex have recombination-independent functions in fork reversal, possibly through physical interactions with SMARCAL1 and ZRANB3.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Di Kang, Guorui Zhang, Zhonghui Zhang, Zhancheng Tian, Shandian Gao, Guangyuan Liu, Guiquan Guan, Jianxun Luo, Hong Yin, Junzheng Du
Summary: This study found that the transcription level of ovine ISG15 (oISG15) is upregulated in a time-dependent and BTV multiplicity of infection-dependent manner. Overexpression of oISG15 enhances BTV replication, while knockdown of endogenous oISG15 inhibits BTV replication. The interaction between BTV viral proteins and oISG15 was identified, and oISG15 overexpression was found to stabilize VP4 and NS1 proteins and interfere with the degradation of NS1 via the autophagy pathway.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Yoshitami Hashimoto, Hirofumi Tanaka
Summary: The study reveals a novel role of Mre11 exonuclease activity in promoting the initial progression of mitotic entry under replication stress and suggests that it facilitates the processing of stalled replication forks, leading to irreversible mitotic progression and replisome disassembly.
LIFE SCIENCE ALLIANCE
(2022)
Article
Microbiology
Qinting Dong, Xinyue Zhu, Leping Wang, Wenchao Zhang, Lifei Lu, Jun Li, Shuhong Zhong, Chunxia Ma, Kang Ouyang, Ying Chen, Zuzhang Wei, Yifeng Qin, Hao Peng, Weijian Huang
Summary: Porcine astrovirus type 1 (PAstV1) induces the production of IFN-beta through the IRF3 signaling pathway and inhibits viral replication. Knockdown of RIG-I and MDA5 results in decreased expression of IFN-beta and increased viral replication induced by PAstV1.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Brian S. Nielsen, Raj R. Malinda, Fabian M. Schmid, Stine F. Pedersen, Soren T. Christensen, Lotte B. Pedersen
JOURNAL OF CELL SCIENCE
(2015)
Article
Cell Biology
Fabian Marc Schmid, Kenneth Bodtker Schou, Martin Juel Vilhelm, Maria Schroder Holm, Loretta Breslin, Pietro Farinelli, Lars Allan Larsen, Jens Skorstengaard Andersen, Lotte Bang Pedersen, Soren Tvorup Christensen
JOURNAL OF CELL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Anja Basters, Paul P. Geurink, Annika Roecker, Katharina F. Witting, Roya Tadayon, Sandra Hess, Marta S. Semrau, Paola Storici, Huib Ovaa, Klaus-Peter Knobeloch, Guenter Fritz
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Anja Basters, Paul P. Geurink, Annika Roecker, Katharina F. Witting, Roya Tadayon, Sandra Hess, Marta S. Semrau, Paola Storici, Huib Ovaa, Klaus-Peter Knobeloch, Guenter Fritz
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Jonas Andreas Schmid, Matteo Berti, Franziska Walser, Maria Chiara Raso, Fabian Schmid, Jana Krietsch, Henriette Stoy, Katharina Zwicky, Sebastian Ursich, Raimundo Freire, Massimo Lopes, Lorenza Penengo
Review
Biochemistry & Molecular Biology
Almut Dufner, Klaus-Peter Knobeloch
BIOCHEMICAL SOCIETY TRANSACTIONS
(2019)
Review
Chemistry, Multidisciplinary
Daniel Jimenez Fernandez, Sandra Hess, Klaus-Peter Knobeloch
FRONTIERS IN CHEMISTRY
(2020)
Article
Immunology
Victoria Iglesias-Guimarais, Tomasz Ahrends, Evert de Vries, Klaus-Peter Knobeloch, Andriy Volkov, Jannie Borst
JOURNAL OF IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jorg Schweiggert, Gregor Habeck, Sandra Hess, Felix Mikus, Roman Beloshistov, Klaus Meese, Shoji Hata, Klaus-Peter Knobeloch, Frauke Melchior
Summary: The ubiquitin E3 ligase SCFFbxw5 targets MCAK for proteasomal degradation during G(2), which promotes ciliogenesis starting in the preceding cell cycle phase. Loss of Fbxw5 results in increased MCAK levels at basal bodies and impairs ciliogenesis in the following G(1)/G(0).
Article
Immunology
Namir Shaabani, Nadine Honke, Nhan Nguyen, Zhe Huang, Kei-ichiro Arimoto, Daniel Lazar, Taylor K. Loe, Karl S. Lang, Marco Prinz, Klaus-Peter Knobeloch, Dong-Er Zhang, John R. Teijaro
SCIENCE IMMUNOLOGY
(2018)