期刊
FOOD CHEMISTRY
卷 320, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126581
关键词
Bacterial viability; Bienzyme system; Colorimetric detection; Foodborne pathogen; Drinking water
资金
- National innovation special zone project [18-163-15-LZ-001-002-03]
- Shanghai Agriculture Applied Technology Development Program [G20180101]
- NSFC [81871448]
- State Key Laboratory program [DMETKF2018014]
Foodborne bacterial infection poses a serious threat to human health. As most diseases are caused by living bacteria, real-time assessment of bacterial viability is vitally important to the public health sector. Herein, we developed a simple and novel colorimetric assay based on the Glucose oxidase (GOD)/Horseradish peroxidase (HRP) bienzyme system for real-time monitoring of bacterial viability in food and drinking water. This bienzyme system is free of any chemical synthesis and only requires 3 sample handling steps. The color response is easily observable with the naked eye or recordable with a smartphone for precise determination of bacterial viability. The proposed strategy was validated with various bacteria both Gram-positive and Gram-negative, indicating its capability for broad-spectrum bacteria viability detection. Therefore, the proposed strategy shows promise for rapid and reliable quality control in food and drinking water.
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